Molecules,
Год журнала:
2024,
Номер
29(19), С. 4746 - 4746
Опубликована: Окт. 8, 2024
The
growing
emergence
of
multi-drug
resistant
microbial
strains
has
kept
the
scientific
world
searching
for
novel
bioactive
compounds
with
specific
chemical
characteristics.
Accordingly,
researchers
have
started
exploring
understudied
metabolites
from
endophytes
as
a
new
source
compounds.
In
this
context,
current
study
was
designed
to
evaluate
properties
endophytic
fungi
Mokrzański
forest
in
Wrocław,
Poland
that
not
yet
been
fully
researched.
Forty-three
were
isolated
twelve
distinct
plants.
Following
their
cultivation,
fungal
extracts
separately
prepared
biomass
and
cell-free
filtrates,
antibacterial,
antifungal
(against
human
plant
pathogens),
antioxidant
examined.
Five
promising
after
screening
identified
possess
all
these
activities.
These
respective
hosts
Frontiers in Plant Science,
Год журнала:
2025,
Номер
16
Опубликована: Март 24, 2025
This
study
investigates
the
effects
of
irrigated
and
non-irrigated
conditions
on
bioactive
compound
content
in
fenugreek
(
Trigonella
foenum-graecum
)
across
31
diverse
genotypes
from
various
geographical
regions.
The
was
conducted
at
Atatürk
University
Research
Extension
Center,
Türkiye
(N
39°55’59.9”,
E
41°14’10.6”,
altitude
1789
m)
during
2021
2022
growing
seasons.
levels
diosgenin,
trigonelline,
4-hydroxyisoleucine
analyzed
under
were
found
to
be
significantly
influenced
by
genotype,
environment,
their
interaction
(Genotype
×
Environment),
with
a
highly
significant
effect
observed
p
<
0.001
level.
compounds
included
diosgenin
(0.50-0.93%),
trigonelline
(5.22-13.65
mg
g
-
¹),
(0.41-1.90%).
Notably,
such
as
Sivas/TR,
Amasya/TR,
Konya/TR
Samsun/TR
exhibited
higher
all
conditions,
while
Spain,
Malaysia,
France,
India
showed
irrigation.
Variability
observed,
some
showing
stability
different
environmental
conditions.
A
negative
correlation
between
fenugreek.
Furthermore,
Principal
Component
Analysis
(PCA)
cluster
analysis
effective
revealing
genetic
diversity,
morphological
differences,
genotype
adaptability.
findings
highlight
potential
for
selecting
superior
breeding
programs
focused
enhancing
yields,
especially
varying
irrigation
research
emphasizes
critical
role
factors
optimizing
production
health-benefiting
International Journal of Molecular Sciences,
Год журнала:
2025,
Номер
26(7), С. 3306 - 3306
Опубликована: Апрель 2, 2025
Untapped
bioactive
compounds
from
microbial
endophytes
offer
a
promising
solution
to
counter
antimicrobial
and
chemotherapeutic
drug
resistance
when
complexed
as
silver
nanoparticles
(AgNPs).
AgNPs
were
biosynthesized
using
cell-free
supernatants
endophytic
Streptomyces
sp.
KE4D
Bacillus
safensis
KE4K
isolated
the
Kenyan
medicinal
plant
Teclea
nobilis,
following
fermentation
in
three
different
media.
Bacterial
extracts
analyzed
gas
chromatography-mass
spectrometry.
characterized
Fourier-transform
infrared
spectroscopy
high-resolution
transmission
electron
microscopy.
Antimicrobial
activity
was
assessed
agar
well
diffusion
assays,
quorum
sensing
inhibition
(QSI)
investigated
Chromobacterium
violaceum.
Anti-cancer
potential
evaluated
against
breast
(MCF-7)
prostate
cancer
(DU-145)
cell
lines
MTT
assays.
5-55
nm
size,
with
being
spherical
exhibiting
various
shapes.
Cyclopropane
acetic
acids
fatty
identified
possible
capping
agents.
Medium-dependent
observed,
medium
Mannitol
5294
displaying
stronger
activity,
particularly
Gram-negative
indicators.
demonstrated
85.12%
violacein
at
140
µg/mL
better
QSI
whilst
antimicrobials.
The
IC50
values
<3.5
for
MCF-7
<2.5
DU-145
cells.
bioactivity
of
is
influenced
by
bacterial
isolate
medium,
suggesting
that
AgNP
synthesis
can
be
tailored
specific
bioactivity.
Microorganisms,
Год журнала:
2025,
Номер
13(5), С. 1080 - 1080
Опубликована: Май 6, 2025
Endophytic
fungi
(EF)
play
crucial
roles
in
accelerating
the
accumulation
of
medicinal
components
and
regulating
survival
reproduction
plants.
Erigeron
breviscapus
is
a
well-known
traditional
Chinese
plant
with
effective
therapeutic
effects
wide
application,
but
little
known
about
diversity,
community
structure,
ecological
EF
this
plant.
Here,
communities
associated
different
tissues
E.
at
two
seasons
were
studied
by
high-throughput
sequencing
methods.
Furthermore,
FUNGuild
was
performed
to
predict
functions
fungi.
Didymella
found
be
most
dominant
genus
across
all
four
tissues,
followed
Plectosphaerella,
Filobasidium,
Cystofilobasidium,
Cladosporium.
Notably,
unique
genera
biomarkers
different.
Interestingly,
it
that
roots
had
highest
fungal
richness
diversity
summer.
Moreover,
both
PCoA
plots
PERMANOVA
analyses
indicated
tissue
season
main
factors
contributing
differences
breviscapus.
prediction
revealed
pathotroph–saprotroph
undefined
taxa
accounted
for
high
proportion
We
also
some
valuable
endophytes
encouraged
deeper
investigation.
These
findings
provide
theoretical
reference
further
development
utilization
resources
Molecules,
Год журнала:
2024,
Номер
29(6), С. 1297 - 1297
Опубликована: Март 14, 2024
The
efficacy
of
23
bacterial
isolates
obtained
from
surface-sterilized
stems
and
leaves
three
medicinal
plants
(Aloe
barbadensis
Miller,
Artemisia
afra,
Moringa
oleifera)
was
investigated
in
an
endeavour
to
prevent
the
growth
Mycobacterium
bovis
using
cross-streak
method.
Endophytes
were
isolated
by
incubating
sterile
plant
materials
on
nutrient
agar
at
30
°C
for
5
days.
Two
showing
activity
subsequently
utilized
produce
extracts.
Whole-genome
sequencing
(WGC)
used
identify
isolates.
Secondary
metabolites
produced
after
7
days
broth
harvested
through
extraction
with
ethyl
acetate.
extracts
chemically
profiled
gas
chromatography–high
resolution
time-of-flight
mass
spectrometry
(GC–HRTOF-MS).
NCBI
BLAST
search
results
revealed
that
endophytes
belonged
Pseudomonas
Enterobacter
genera,
based
WGC.
endophytes,
Aloe
I4
I3–I5
barbadensis,
exhibited
potency
metabolite
profiling
selected
identified
34
I4,
including
ergotamine,
octadecane,
L-proline
143
other
quinoline
valeramide,
which
inhibit
microbial
quorum
sensing.
These
findings
suggest
plants,
particularly
hold
promise
as
sources
antimycobacterial
agents
human
health
applications.
Current Journal of Applied Science and Technology,
Год журнала:
2023,
Номер
42(46), С. 7 - 22
Опубликована: Дек. 1, 2023
Plant
tissue
culture
is
emerging
as
a
pivotal
biotechnological
tool
with
profound
implications
for
medical
therapy,
particularly
in
the
realm
of
herbal
medicine.
Medicinal
plants
have
long
been
cherished
their
natural
healing
properties.
However,
escalating
demand,
habitat
destruction,
and
overharvesting
threatened
availability
sustainability
these
valuable
resources.
addresses
concerns
by
enabling
mass
propagation
medicinal
plants.
In
controlled
environments,
plant
tissues
can
be
multiplied
rapidly,
providing
continuous
sustainable
source
material.
This
not
only
safeguards
wild
populations
but
also
ensures
consistent
supply
bioactive
compounds
that
form
basis
therapies.
One
most
transformative
applications
therapy
manipulation
secondary
metabolite
production.
synthesize
diverse
array
compounds,
such
alkaloids,
flavonoids,
terpenoids,
therapeutic
Through
precise
control
growth
conditions
genetic
modification,
enhance
yield
thereby
increasing
potency
efficacy
medicines.
precision
instrumental
pharmaceutical
industry,
where
isolation
production
specific
lead
to
development
novel
drugs
addition
bolstering
yields,
offers
advantage
disease-free
By
maintaining
cultures
sterile
conditions,
risk
contaminants
pathogens
mitigated,
enhancing
safety
quality
These
serve
plant-derived
substances.
Furthermore,
crucial
research
field
It
provides
platform
studying
biology,
optimizing
investigating
mechanisms
underlying
insights
contribute
improved
varieties
enhanced
properties,
addressing
evolving
needs
therapy.
While
potential
vast,
it
essential
underscore
importance
rigorous
research,
control,
assessments.
Ensuring
products
derived
from
tissue-cultured
paramount
acceptance
use
applications.
Compliance
regulatory
standards
collaboration
healthcare
professionals
are
integral
upholding
products.
