LIGHt-based rapid detection of starch in tobacco leaves by smartphone sensing

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Фев. 25, 2025

A rapid and efficient detection method to detect the total starch in tobacco leaves using smartphone-based anthrone-sulfuric acid colorimetry is established under framework of low-cost, immediate, general-purpose, high-throughput (LIGHt) colorimetry. The LIGHt smartphone resulted average relative error 5.74%, with a standard deviation (RSD) ranging from 2.58 4.31%, limit 1.53 µg/mL, an recovery rate 95.72%. results demonstrated that perform comparably regular visible spectrophotometry determining concentration, validating feasibility this novel colorimetric method, which requires no specific instruments. LIGHt-based indicated potential for application field quality plant other food medicinal-related control practices.

Язык: Английский

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Evolution of plant cell-type-specificcis-regulatory elements

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Янв. 9, 2024

Abstract Cis -regulatory elements (CREs) are critical in regulating gene expression, and yet understanding of CRE evolution remains challenging. Here, we constructed a comprehensive single-cell atlas chromatin accessibility Oryza sativa , integrating data from 103,911 nuclei representing 126 discrete cell states across nine distinct organs. We used comparative genomics to compare cell-type resolved between O. 57,552 four additional grass species ( Zea mays, Sorghum bicolor, Panicum miliaceum Urochloa fusca ). Accessible regions (ACRs) had different levels conservation depending on the degree specificity. found complex relationship ACRs with conserved noncoding sequences, specificity, conservation, tissue-specific switching. Additionally, that epidermal were less compared other types, potentially indicating more rapid regulatory has occurred L1-derived layer these species. Finally, identified characterized subset overlapped repressive histone modification H3K27me3, implicating them as silencer-like CREs maintained by evolution. Collectively, this approach highlights dynamics plant cell-type-specific

Язык: Английский

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Better estimation of evapotranspiration and transpiration using an improved modified Priestly-Taylor model based on a new parameter of leaf senescence in a rice field

The Science of The Total Environment, Год журнала: 2024, Номер 927, С. 171842 - 171842

Опубликована: Март 20, 2024

Язык: Английский

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Understanding Cytokinin: Biosynthesis, Signal Transduction, Growth Regulation, and Phytohormonal Crosstalk under Heavy Metal Stress

Environmental and Experimental Botany, Год журнала: 2024, Номер 228, С. 106025 - 106025

Опубликована: Ноя. 2, 2024

Язык: Английский

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Positive impacts of typical desert photovoltaic scenarios in China on the growth and physiology of sand-adapted plants

Frontiers in Plant Science, Год журнала: 2025, Номер 15

Опубликована: Янв. 20, 2025

Desert regions are characterized by complex terrain, frequent wind–sand activities, and extreme environmental conditions making vegetation recovery after disturbances difficult. The construction of large-scale photovoltaic (PV) power stations presents a significant challenge in balancing with protection. This study focused on large PV site the Hobq examining growth characteristics Astragalus adsurgens at different positions within fixed arrays. It also analyzed changes chlorophyll (Chl), soluble sugar (SS), protein (SP), enzyme activity, other physiological indicators to evaluate plant’s adaptive mechanisms presence panels. results showed that A. near panels exhibited advantages, plant height, leaf length, stem–leaf nutrient content greater than those control (CK) showing an trend elongation, thinning, enlargement. During growing season, located before, behind, under increased Chl changes. plants adjusted their SS, SP, internal substance levels depending location relative Notably, superoxide dismutase (SOD) peroxidase (POD) activities were higher all treated compared CK, effectively removing O 2− providing H 2 protection, thereby delaying senescence demonstrating strong adaptability. Through membership function analysis, tolerance various around ranked > before behind CK. In conclusion, demonstrated adaptability modifying its traits. These findings provide scientific evidence for ecological industrial use desert offer practical guidance restoration such stations.

Язык: Английский

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Identification and Functional Analysis of Candidate Genes Influencing Citrus Leaf Size Through Transcriptome and Coexpression Network Approaches

Genes, Год журнала: 2025, Номер 16(1), С. 97 - 97

Опубликована: Янв. 17, 2025

Background: Leaves are the main organs involved in photosynthesis. They capture light energy and promote gas exchange, their size shape affect yield. Identifying regulatory networks key genes that control citrus leaf is essential for increasing crop Methods: In this study, transcriptome sequencing was performed on three materials: ‘Cuimi’ kumquat (Nor) variety its variants, larger-leaf (VarB) smaller-leaf (VarS) varieties. Results: Correlation principal component analyses revealed a relatively close correlation between Nor VarS. A total of 7264 differentially expressed (DEGs), including 2374 transcription factors (TFs), were identified, 254 DEGs common among materials. GO KEGG enrichment significant glucose metabolism, cell wall composition, starch biosynthesis, photosynthesis pathways. WGCNA identified specific modules related to different sizes these Fifteen candidate size, factors, Fh5g30470 (MYB), Fh7g07360 (AP2/ERF), Fh5g02470 (SAP), basis connectivity functional annotations. Conclusions: These findings provide theoretical foundation deeper understanding molecular mechanisms underlying offer new genetic resources study size.

Язык: Английский

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Detection of Colombian datura virus infecting Brugmansia × candida medicinal cultivars and evaluation of sap inoculation in Solanaceae plants

Research Square (Research Square), Год журнала: 2025, Номер unknown

Опубликована: Янв. 23, 2025

Angel’s trumpets (Brugmansia spp.) are important medicinal plants belonging to the Solanaceae family that were domesticated in Andean region of South America. Indigenous communities from Colombian Andes have created cultivars with unusual leaf shapes and different uses. These exhibit symptoms on leaves associated viral infection, such as mosaic, vein chlorosis, morphological deformities. Previous studies shown most widespread virus Brugmansia is Colombian datura virus (CDV), a globally distributed potyvirus also affects agriculturally significant hosts. The present study aimed evaluate health status related infections relationship between presence viruses expression their leaves. We searched for CDV transcriptomes diverse collection species found it mainly wild solanaceous species. Sap B. × candida used source inoculum infect representative cultivated family. confirmed infection mechanically inoculated by RT-PCR sequencing. Furthermore, we ability cause deformations Physalis peruviana, Solanum lycopersicum, first time, reported melongena. In conclusion, this pioneering characterizing involved domestication plant.

Язык: Английский

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Spatial Regulation of Rice Leaf Morphology by miRNA‐Target Complexes During Viral Infection

Plant Cell & Environment, Год журнала: 2025, Номер unknown

Опубликована: Март 5, 2025

Leaf morphogenesis is essential for plant growth and development, yet the mechanisms by which viruses induce changes in leaf shape are not well understood. Rice ragged stunt virus (RRSV) infection induces distinct morphological abnormalities rice leaves, including tip curling serrated margins, through unknown pathogenic mechanisms. This study reveals that key regulatory microRNAs (miR164, miR319 miR156) their target genes (CUC, TCP SPL) exhibit entirely opposite expression patterns healthy RRSV-infected indicating a profound impact on network. Significantly, core protein OsCUC1, typically functions forming dimers, shows abnormal peripheral zone of shoot apical meristem under viral infection, leading to disruptions development. OsTCP1 was found dynamically regulate OsCUC1 dimer formation modifying its subcellular localization interacting with OsSPL14 OsSPL17, thereby influencing functions. Genetic OsSPL14/OsSPL17 enhance severity RRSV demonstrating critical involvement strategy. The research uncovers novel mechanism manipulates interactions factors, disrupting delicate balance These findings expand our understanding manipulation host development provide foundation innovative strategies crop resilience.

Язык: Английский

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Single-cell RNA sequencing reveals transcriptional regulation and metabolic pathways of terpenoid biosynthesis in developing Cinnamomum camphora leaf cells

Current Plant Biology, Год журнала: 2025, Номер unknown, С. 100467 - 100467

Опубликована: Март 1, 2025

Язык: Английский

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A space for time. Exploring temporal regulation of plant development across spatial scales

The Plant Journal, Год журнала: 2025, Номер 122(1)

Опубликована: Март 31, 2025

SUMMARY Plants continuously undergo change during their life cycle, experiencing dramatic phase transitions altering plant form, and regulating the assignment progression of cell fates. The relative timing developmental events is tightly controlled involves integration environmental, spatial, age‐related signals actors. While have been studied extensively many regulators described, less known about temporal regulation on a smaller, cell‐level scale. Here, using examples from both animal systems, we outline time‐dependent changes. Looking at systemic scale changes, discuss germination, juvenile‐to‐adult transition, flowering, senescence, together with regeneration timing. Switching to cellular level, several instances field in which control has examined this Then, switch back plants summarize where changes are temporally regulated. As time cannot easily be separated signaling derived environment tissue context, next factors that implicated controlling events, reviewing temperature, photoperiod, nutrient availability, as well context mechanical cues Afterwards, provide an overview mechanisms shown or development, considering metabolism, division control, mobile signals, epigenetic regulation, action transcription factors. Lastly, look remaining questions for future study how recent technical advancement can enable these efforts.

Язык: Английский

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