Pinpointing the timing of meiosis: a critical factor in evaluating the impact of abiotic stresses on the fertility of cereal crops DOI
Farhad Masoomi‐Aladizgeh, Brian J. Atwell, A. I. Bokshi

и другие.

New Phytologist, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 22, 2024

Summary The development of male gametes, vital to sexual reproduction in crops, requires meiosis followed by successive mitotic cell divisions haploid cells. formation viable pollen is especially vulnerable abiotic stress, with consequences both for yield and grain quality. An understanding key molecular responses when specific stages during are subjected stress (e.g. heat) possible only sampling carefully informed developmental biology. Traditionally, morphological characteristics have been commonly used cereals as ‘indicators’ reproductive stages. We argue that these attributes strongly influenced genotype genotype–environment interactions cannot be reliably define events microsporogenesis microgametogenesis. Furthermore, asynchronous along the axis a single inflorescence calls selective individual florets accurately. therefore propose guidelines standardise cells development, particularly interrogating impact on susceptible meiosis. Improved knowledge will largely negate variability imposed genotype, environment florets. Highlighting subtleties required investigation make selection stress‐tolerant cereal genotypes more reliable.

Язык: Английский

Heating up meiosis – Chromosome recombination and segregation under high temperatures DOI Creative Commons
Joke De Jaeger‐Braet, Arp Schnittger

Current Opinion in Plant Biology, Год журнала: 2024, Номер 80, С. 102548 - 102548

Опубликована: Май 14, 2024

Heat stress is one of the major constraints to plant growth and fertility. During current climate crisis, heat waves have increased dramatically, even more extreme conditions are predicted for near future, considerably affecting ecosystems seriously threatening world food security. Although very well known affect especially reproductive structures, little about how interferes with reproduction in comparison somatic cells tissues. Recently, effect on meiosis as a central process sexual has been analyzed molecular cytological depth. Notably, these studies not only important applied research by laying foundation breeding heat-resilient crops, but also fundamental research, revealing general regulatory mechanisms recombination chromosome segregation control.

Язык: Английский

Процитировано

6
The tale of tapetum: from anther walls to pollen wall DOI
Ruby Biswas, Shubho Chaudhuri

The Nucleus, Год журнала: 2024, Номер 67(3), С. 611 - 630

Опубликована: Авг. 21, 2024

Язык: Английский

Процитировано

5
Heat stress-induced meiotic restitution and tissue-specific gene responses in the sterile Lilium OT Hybrid ‘Yelloween’ DOI Creative Commons
Yun‐Jae Ahn, Ji-Yun Kang, Ki‐Byung Lim

и другие.

Scientia Horticulturae, Год журнала: 2025, Номер 342, С. 114032 - 114032

Опубликована: Фев. 1, 2025

Язык: Английский

Процитировано

0
TransGeneSelector: using a transformer approach to mine key genes from small transcriptomic datasets in plant responses to various environments DOI Creative Commons
Kerui Huang,

Jianhong Tian,

Лэй Сун

и другие.

BMC Genomics, Год журнала: 2025, Номер 26(1)

Опубликована: Март 17, 2025

Gene mining is crucial for understanding the regulatory mechanisms underlying complex biological processes, particularly in plants responding to environmental conditions. Traditional machine learning methods, while useful, often overlook important gene relationships due their reliance on manual feature selection and limited ability capture inter-gene dynamics. Deep approaches, powerful, are unsuitable small sample sizes. This study introduces TransGeneSelector, first deep framework specifically designed key genes from transcriptomic datasets. By integrating a Wasserstein Generative Adversarial Network with Gradient Penalty (WGAN-GP) generation Transformer-based network classification, TransGeneSelector efficiently addresses challenges of small-sample data, capturing both global interactions specific processes. Evaluated Arabidopsis thaliana, model achieved high classification accuracy predicting seed germination heat stress conditions, outperforming traditional methods like Random Forest Support Vector Machines (SVM). Moreover, Shapley Additive Explanations (SHAP) analysis construction revealed that effectively identified appear have upstream functions based our analyses, enriching them multiple pathways which critical response. RT-qPCR validation further confirmed model's accuracy, demonstrating consistent expression patterns across varying The findings underscore potential as robust tool mining, offering deeper insights into regulation organism adaptation under diverse work provides leverages identification

Язык: Английский

Процитировано

0
Genetic effect of the Ph1 locus on transcriptome atlas of anther development‐related genes, meiotic chromosome behavior and agronomic traits in bread wheat DOI

Renyi Zhou,

Li Guo, Tong Feng

и другие.

The Plant Journal, Год журнала: 2025, Номер 122(3)

Опубликована: Май 1, 2025

SUMMARY Proper spatiotemporal expression of meiosis‐related genes (MRGs) and other male‐microsporogenesis/microgametogenesis‐related (MMRGs) is crucial for normal anther development, yet their patterns remain largely unknown in wheat. The Ph1 locus wheat known to contain the gene that plays a dual role promoting pairing between homologous chromosomes but repressing homoeologous chromosomes, its genetic function still unclear. Here, we investigated these issues by conducting comprehensive transcriptome analysis during development Chinese Spring (CS) ph1b deletion mutant under greenhouse field conditions. Our results revealed MRGs MMRGs are predominantly expressed pre‐meiosis stages, with also being highly meiotic‐II. Gene co‐expression showed C2H2 B3 transcriptional factors (TFs) associated MRGs, MYB regulators interacted mainly microgametogenesis. Deletion within failed induce compensatory activation counterparts, while outside environmental‐specific responses, especially meiotic‐II mature pollen stages. Notably, early disjunction bivalent primary factor leading defective meiocytes metaphase I. Furthermore, exhibited substantially delayed heading date, potentially contributing environment‐stable environment‐specific alterations fertility grain‐related traits. study highlights significant impact on previously unheeded effect meiotic chromosome agronomic traits, suggesting potential manipulations improvement.

Язык: Английский

Процитировано

0
Heat-Triggered Transcriptional Reprogramming in Microspores Disrupts Progression of Pollen Development in Brassica napus L. DOI Creative Commons
Neeta Lohani, Mohan B. Singh, Prem L. Bhalla

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Ноя. 17, 2024

Abstract Rising temperatures due to climate change pose significant threats global food security, particularly by impacting crop fertility and yield. Despite the known susceptibility of male reproductive development heat stress, specific adaptive mechanisms critical regulatory genes involved in stage-specific stress response crops are still not well understood. This study examines impact acute on pollen Brassica napus (Canola), an essential oilseed crop, with a focus uninucleate microspore stage. We demonstrate that brief exposure 40°C during stage results loss cellular polarity, failed asymmetric cell division, cytoplasm, abortion, leading decline viability. Transcriptome analysis reveals complexity heat-induced transcriptional reprogramming microspores, identifying 8.1% (6,245) expressed as responsive high temperature, higher degree downregulation. Fourteen differentially transcription factors (TFs) belonging diverse TF families were identified heat-stressed overrepresented target genes. The differential regulation cycle control microspores supports our microscopic observations details underlying failure undergo first second mitotic divisions (PMI PMII), resulting aberrant B. . Heat disrupts key biological pathways such transcription, mRNA processing, translational control, protein homeostasis, autophagy, phytohormone signaling, reactive oxygen species (ROS) homeostasis. alters intricate network multiple signaling pathways, potentially disrupting development, evident crosstalk underscoring development. These findings underscore vulnerability Canola. Understanding heat-responsive networks provides valuable insights for breeding climate-resilient Canola varieties, thereby contributing security amidst changing climatic conditions. Highlights Acute induces complex Transcriptional RNA coordination homeostasis is altered Misregulation polarity division stressed microspores.

Язык: Английский

Процитировано

2
Deciphering the Vulnerability of Pollen to Heat Stress for Securing Crop Yields in a Warming Climate DOI
Neeta Lohani, Mohan B. Singh, Prem L. Bhalla

и другие.

Plant Cell & Environment, Год журнала: 2024, Номер unknown

Опубликована: Дек. 25, 2024

ABSTRACT Climate change is leading to more frequent and severe extreme temperature events, negatively impacting agricultural productivity threatening global food security. Plant reproduction, the process fundamental crop yield, highly susceptible heatwaves, which disrupt pollen development ultimately affect seed‐set yields. Recent research has increasingly focused on understanding how grains from various crops react heat stress at molecular cellular levels. This surge in interest over last decade been driven by advances genomic technologies, such as single‐cell RNA sequencing, holds significant potential for revealing underlying regulatory reprogramming triggered throughout stages of development. review focuses affects gene networks, including response, unfolded protein autophagy, discusses impact these changes It highlights selection a key strategy improving tolerance leveraging genetic variability among grains. Additionally, genome‐wide association studies population screenings have shed light underpinnings traits major that respond high temperatures during male reproductive stages. Gene‐editing tools like CRISPR/Cas systems could facilitate precise modifications boost resilience. The information covered this valuable selecting employing approaches develop heat‐tolerant genotypes.

Язык: Английский

Процитировано

2
Unlocking fertility in the female gametophyte: a DEAD-box RNA helicase is essential for embryo sac development and seed setting DOI Creative Commons
Asif Ali, Asad Riaz,

Xianjun Wu

и другие.

Journal of Experimental Botany, Год журнала: 2024, Номер 75(16), С. 4684 - 4688

Опубликована: Авг. 22, 2024

This article comments on: Huang J, Qiao Z, Yu H, Lu Chen W, Wu Bao Y, Shahid MQ, Liu X. 2024. OsRH52A, a DEAD-box protein, regulates functional megaspore specification and is required for embryo sac development in rice. Journal of Experimental Botany 75, 4802–4821. https://doi.org/10.1093/jxb/erae180

Язык: Английский

Процитировано

0
Field versus controlled environmental experiments to evaluate the heat stress response of barley (Hordeum vulgare L.) DOI Creative Commons
Ádám Horváth,

Zita Berki,

Krisztina Balla

и другие.

Environmental and Experimental Botany, Год журнала: 2024, Номер 228, С. 106038 - 106038

Опубликована: Ноя. 17, 2024

Язык: Английский

Процитировано

0
Pinpointing the timing of meiosis: a critical factor in evaluating the impact of abiotic stresses on the fertility of cereal crops DOI
Farhad Masoomi‐Aladizgeh, Brian J. Atwell, A. I. Bokshi

и другие.

New Phytologist, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 22, 2024

Summary The development of male gametes, vital to sexual reproduction in crops, requires meiosis followed by successive mitotic cell divisions haploid cells. formation viable pollen is especially vulnerable abiotic stress, with consequences both for yield and grain quality. An understanding key molecular responses when specific stages during are subjected stress (e.g. heat) possible only sampling carefully informed developmental biology. Traditionally, morphological characteristics have been commonly used cereals as ‘indicators’ reproductive stages. We argue that these attributes strongly influenced genotype genotype–environment interactions cannot be reliably define events microsporogenesis microgametogenesis. Furthermore, asynchronous along the axis a single inflorescence calls selective individual florets accurately. therefore propose guidelines standardise cells development, particularly interrogating impact on susceptible meiosis. Improved knowledge will largely negate variability imposed genotype, environment florets. Highlighting subtleties required investigation make selection stress‐tolerant cereal genotypes more reliable.

Язык: Английский

Процитировано

0