Exogenous dsRNA-Mediated RNAi: Mechanisms, Applications, Delivery Methods and Challenges in the Induction of Viral Disease Resistance in Plants
Viruses,
Год журнала:
2024,
Номер
17(1), С. 49 - 49
Опубликована: Дек. 31, 2024
The
increasing
challenges
posed
by
plant
viral
diseases
demand
innovative
and
sustainable
management
strategies
to
minimize
agricultural
losses.
Exogenous
double-stranded
RNA
(dsRNA)-mediated
interference
(RNAi)
represents
a
transformative
approach
combat
pathogens
without
the
need
for
genetic
transformation.
This
review
explores
mechanisms
underlying
dsRNA-induced
RNAi,
highlighting
its
ability
silence
specific
genes
through
small
interfering
RNAs
(siRNAs).
Key
advancements
in
dsRNA
production,
including
cost-effective
microbial
synthesis
vitro
methods,
are
examined
alongside
delivery
techniques
such
as
spray-induced
gene
silencing
(SIGS)
nanocarrier-based
systems.
Strategies
enhancing
stability,
use
of
nanomaterials
like
layered
double
hydroxide
nanosheets
carbon
dots,
discussed
address
environmental
degradation
challenges.
Practical
applications
this
technology
against
various
viruses
potential
ensure
food
security
emphasized.
also
delves
into
regulatory
considerations,
risk
assessments,
associated
with
off-target
effects
pathogen
resistance.
By
evaluating
both
opportunities
limitations,
underscores
role
exogenous
solution
achieving
disease
resistance
plants.
Язык: Английский
Rapid colorimetric detection ofcitrus tristeza viruscombining portable sample preparation and reverse transcription-loop mediated isothermal amplification
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Ноя. 11, 2024
Abstracts
A
sensing
platform
combining
semi-automated
sample
preparation
protocol
and
one-step
reverse
transcription
loop-mediated
isothermal
amplification
(RT-LAMP)
is
reported
for
rapid
colorimetric
detection
of
citrus
tristeza
virus
(CTV)
in
a
greenhouse.
An
OmniLyse
micro-homogenizer
cellulose
paper
disks
were
integrated
quick
total
nucleic
acids
(<15
min).
RT-LAMP
assays
optimized
terms
primers’
concentrations
minimization
false
positives
both
CTV
cytochrome
oxidase
(COX)
detections.
Specifically,
the
optimal
reaction
time
lab-based
was
determined
as
40
minutes
with
limits
COX
43
copies/μL
(equivalent
to
86
copies/mg
tissue)
5
10
tissue),
respectively.
Additionally,
an
in-greenhouse
assay
lyophilized
mix
endpoint
successfully
conducted
35
without
response
either
or
fluorometric
assays.
Overall,
this
showed
high
efficiency
reliability
plant
pathogen
This
strategy
holds
great
potential
be
into
portable,
autonomous
system
universally
adopted
in-field
diagnosis
different
pathogens.
Язык: Английский
Viruses Infecting Yam in Africa: Existing Knowledge and Future Perspectives
Asian Journal of Plant Pathology,
Год журнала:
2024,
Номер
18(1), С. 86 - 102
Опубликована: Дек. 2, 2024
Язык: Английский
Validation of a Novel Strategy for Fluorescence Quenching for a Self-Quenching Fluorogenic Probe and Its Application for Visual Loop-Mediated Isothermal Amplification Detection During Food Safety Analysis
Foods,
Год журнала:
2024,
Номер
13(23), С. 3816 - 3816
Опубликована: Ноя. 26, 2024
The
self-quenching
fluorogenic
probe
facilitates
precise
identification
of
LAMP
(loop-mediated
isothermal
amplification)
amplicons,
unaffected
by
non-specific
products
resulting
from
primer
dimers.
However,
low
quenching
efficiency
surrounding
nucleobases
leads
to
high
background
signal,
posing
significant
challenges
for
visual
inspection
with
the
naked
eye.
present
study
aims
identify
an
oligonucleotide
sequence
that
is
complementary
probe,
and
employ
fluorescence
super-quenching
mechanism
double-stranded
DNA
establish
a
visualization
system
assay.
results
indicated
incorporation
fully
could
significantly
reduce
system’s
(p
<
0.05).
When
melting
temperature
exceeds
room
temperature,
truncating
3′
end
does
not
compromise
probe’s
efficiency.
system,
using
10–13-base
loop
primer-based
effectively
minimize
yield
straightforward
post-reaction.
Applied
rainbow
trout
Atlantic
salmon
detection,
detected
1
pg
in
closed-tube
format.
In
conclusion,
suitable
can
probe.
Employing
this
alongside
develop
low-background
demonstrates
great
potential
successful
detection
holds
considerable
promotional
merit.
Язык: Английский