Validation of a Novel Strategy for Fluorescence Quenching for a Self-Quenching Fluorogenic Probe and Its Application for Visual Loop-Mediated Isothermal Amplification Detection During Food Safety Analysis DOI Creative Commons
Huang Sisi,

Shihui Wang,

Tianlong Wang

et al.

Foods, Journal Year: 2024, Volume and Issue: 13(23), P. 3816 - 3816

Published: Nov. 26, 2024

The self-quenching fluorogenic probe facilitates precise identification of LAMP (loop-mediated isothermal amplification) amplicons, unaffected by non-specific products resulting from primer dimers. However, low quenching efficiency surrounding nucleobases leads to high background signal, posing significant challenges for visual inspection with the naked eye. present study aims identify an oligonucleotide sequence that is complementary probe, and employ fluorescence super-quenching mechanism double-stranded DNA establish a visualization system assay. results indicated incorporation fully could significantly reduce system’s (p < 0.05). When melting temperature exceeds room temperature, truncating 3′ end does not compromise probe’s efficiency. system, using 10–13-base loop primer-based effectively minimize yield straightforward post-reaction. Applied rainbow trout Atlantic salmon detection, detected 1 pg in closed-tube format. In conclusion, suitable can probe. Employing this alongside develop low-background demonstrates great potential successful detection holds considerable promotional merit.

Language: Английский

Exogenous dsRNA-Mediated RNAi: Mechanisms, Applications, Delivery Methods and Challenges in the Induction of Viral Disease Resistance in Plants DOI Creative Commons
Emmadi Venu,

Akurathi Ramya,

Pedapudi Lokesh Babu

et al.

Viruses, Journal Year: 2024, Volume and Issue: 17(1), P. 49 - 49

Published: Dec. 31, 2024

The increasing challenges posed by plant viral diseases demand innovative and sustainable management strategies to minimize agricultural losses. Exogenous double-stranded RNA (dsRNA)-mediated interference (RNAi) represents a transformative approach combat pathogens without the need for genetic transformation. This review explores mechanisms underlying dsRNA-induced RNAi, highlighting its ability silence specific genes through small interfering RNAs (siRNAs). Key advancements in dsRNA production, including cost-effective microbial synthesis vitro methods, are examined alongside delivery techniques such as spray-induced gene silencing (SIGS) nanocarrier-based systems. Strategies enhancing stability, use of nanomaterials like layered double hydroxide nanosheets carbon dots, discussed address environmental degradation challenges. Practical applications this technology against various viruses potential ensure food security emphasized. also delves into regulatory considerations, risk assessments, associated with off-target effects pathogen resistance. By evaluating both opportunities limitations, underscores role exogenous solution achieving disease resistance plants.

Language: Английский

Citations

1
Rapid colorimetric detection ofcitrus tristeza viruscombining portable sample preparation and reverse transcription-loop mediated isothermal amplification DOI Creative Commons
Chia‐Wei Liu,

Sohrab Bodaghi,

Manjunath L. Keremane

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 11, 2024

Abstracts A sensing platform combining semi-automated sample preparation protocol and one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) is reported for rapid colorimetric detection of citrus tristeza virus (CTV) in a greenhouse. An OmniLyse micro-homogenizer cellulose paper disks were integrated quick total nucleic acids (<15 min). RT-LAMP assays optimized terms primers’ concentrations minimization false positives both CTV cytochrome oxidase (COX) detections. Specifically, the optimal reaction time lab-based was determined as 40 minutes with limits COX 43 copies/μL (equivalent to 86 copies/mg tissue) 5 10 tissue), respectively. Additionally, an in-greenhouse assay lyophilized mix endpoint successfully conducted 35 without response either or fluorometric assays. Overall, this showed high efficiency reliability plant pathogen This strategy holds great potential be into portable, autonomous system universally adopted in-field diagnosis different pathogens.

Language: Английский

Citations

0
Viruses Infecting Yam in Africa: Existing Knowledge and Future Perspectives DOI Open Access
Emmanuel Bwanampongo Kulimushi,

Paul Kusolwa,

Espoir Basengere Bisimwa

et al.

Asian Journal of Plant Pathology, Journal Year: 2024, Volume and Issue: 18(1), P. 86 - 102

Published: Dec. 2, 2024

Language: Английский

Citations

0
Validation of a Novel Strategy for Fluorescence Quenching for a Self-Quenching Fluorogenic Probe and Its Application for Visual Loop-Mediated Isothermal Amplification Detection During Food Safety Analysis DOI Creative Commons
Huang Sisi,

Shihui Wang,

Tianlong Wang

et al.

Foods, Journal Year: 2024, Volume and Issue: 13(23), P. 3816 - 3816

Published: Nov. 26, 2024

The self-quenching fluorogenic probe facilitates precise identification of LAMP (loop-mediated isothermal amplification) amplicons, unaffected by non-specific products resulting from primer dimers. However, low quenching efficiency surrounding nucleobases leads to high background signal, posing significant challenges for visual inspection with the naked eye. present study aims identify an oligonucleotide sequence that is complementary probe, and employ fluorescence super-quenching mechanism double-stranded DNA establish a visualization system assay. results indicated incorporation fully could significantly reduce system’s (p < 0.05). When melting temperature exceeds room temperature, truncating 3′ end does not compromise probe’s efficiency. system, using 10–13-base loop primer-based effectively minimize yield straightforward post-reaction. Applied rainbow trout Atlantic salmon detection, detected 1 pg in closed-tube format. In conclusion, suitable can probe. Employing this alongside develop low-background demonstrates great potential successful detection holds considerable promotional merit.

Language: Английский

Citations

0