A Comprehensive Analysis of the Alternative Splicing Co-Factor U2AF65B Gene Family Reveals Its Role in Stress Responses and Root Development
International Journal of Molecular Sciences,
Год журнала:
2025,
Номер
26(8), С. 3901 - 3901
Опубликована: Апрель 20, 2025
U2AF65,
a
65
kDa
splicing
co-factor,
promotes
spliceosome
assembly.
Although
its
role
in
alternative
(AS)
is
known,
the
function
of
U2AF65B
(the
large
subunit
U2AF65)
remains
unclear.
Therefore,
we
systematically
identified
and
analyzed
gene
family
across
36
plant
species,
revealing
103
putative
members
with
conserved
structures
functions.
Phylogenetic
analysis
divided
genes
into
two
clades
five
subgroups,
indicating
evolutionary
divergence.
Gene
structure
motif
analyses
showed
that
most
have
complex
shared
similar
motifs.
Homology
modeling
amino
acid
conservation
revealed
significant
sequences,
particularly
Groups
D
E.
Cis-acting
element
indicated
respond
to
various
stimuli,
supported
by
expression
under
different
stress
conditions.
Subcellular
localization
predictions
proteins
primarily
localize
nucleus
cytoplasm.
Alternative
profile
AS
frequency
likely
varies
between
species.
Functional
AtU2AF65B
mutant
Arabidopsis
loss
enhances
root
elongation
attenuates
ABA-dependent
germination
suppression,
negatively
regulated
seedling
growth
development.
These
findings
provide
insights
history,
molecular
mechanisms,
functional
roles
plants.
Язык: Английский
Alfalfa Photosynthesis Under Partial Root-Zone Drying: Diurnal Patterns and Its Non-Stomatal Limitations
Plants,
Год журнала:
2025,
Номер
14(11), С. 1573 - 1573
Опубликована: Май 22, 2025
The
effects
of
stomatal
factors
plant
leaves
under
partial
root-zone
drying
(PRD)
have
been
widely
studied.
However,
the
non-stomatal
and
relationship
between
photosynthesis
with
soil
moisture
not
explored.
In
this
study,
four
treatments
over-irrigation,
full
irrigation,
moderate
water
deficit,
severe
deficit
were
investigated,
aiming
to
evaluate
on
diurnal
variation
alfalfa
leaf
PRD
its
limitations,
as
well
moisture.
results
showed
that
any
levels
led
a
decrease
in
photosynthetic
rate
(Pn)
leaves.
Leaves
displayed
pronounced
midday
“photosynthetic
lunch
break,”
while
those
over-
irrigation
did
display
phenomenon.
Before
11:30
a.m.,
reduction
Pn
was
primarily
due
evidenced
by
reduced
conductance
(Gs)
decreased
intercellular
CO2
concentration
(Ci).
After
limitations
became
dominant
factor,
both
Gs
transpiration
(Tr)
continuing
decrease,
Ci
increased,
indicating
shift
limiting
factors.
Under
experienced
within
single
day,
leading
hay
yield
18.6%.
Additionally,
over-irrigation
helped
maintain
higher
Tr,
increasing
thus
improving
productivity
33.1%.
correlation
coefficients
content
at
10
cm
depths
Pn,
measurement
day
0.9864,
0.8571,
0.8462,
respectively.
At
20
cm,
0.8820,
0.6943,
0.6951,
study
concluded
mechanisms
contributed
PRD.
Furthermore,
shallow
also
played
crucial
role
influencing
performance.
Язык: Английский