A Comprehensive Analysis of the Alternative Splicing Co-Factor U2AF65B Gene Family Reveals Its Role in Stress Responses and Root Development DOI Open Access
Xiangfeng Meng, Yongzhou Wang, Bei Tang

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(8), P. 3901 - 3901

Published: April 20, 2025

U2AF65, a 65 kDa splicing co-factor, promotes spliceosome assembly. Although its role in alternative (AS) is known, the function of U2AF65B (the large subunit U2AF65) remains unclear. Therefore, we systematically identified and analyzed gene family across 36 plant species, revealing 103 putative members with conserved structures functions. Phylogenetic analysis divided genes into two clades five subgroups, indicating evolutionary divergence. Gene structure motif analyses showed that most have complex shared similar motifs. Homology modeling amino acid conservation revealed significant sequences, particularly Groups D E. Cis-acting element indicated respond to various stimuli, supported by expression under different stress conditions. Subcellular localization predictions proteins primarily localize nucleus cytoplasm. Alternative profile AS frequency likely varies between species. Functional AtU2AF65B mutant Arabidopsis loss enhances root elongation attenuates ABA-dependent germination suppression, negatively regulated seedling growth development. These findings provide insights history, molecular mechanisms, functional roles plants.

Language: Английский

A Comprehensive Analysis of the Alternative Splicing Co-Factor U2AF65B Gene Family Reveals Its Role in Stress Responses and Root Development DOI Open Access
Xiangfeng Meng, Yongzhou Wang, Bei Tang

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(8), P. 3901 - 3901

Published: April 20, 2025

U2AF65, a 65 kDa splicing co-factor, promotes spliceosome assembly. Although its role in alternative (AS) is known, the function of U2AF65B (the large subunit U2AF65) remains unclear. Therefore, we systematically identified and analyzed gene family across 36 plant species, revealing 103 putative members with conserved structures functions. Phylogenetic analysis divided genes into two clades five subgroups, indicating evolutionary divergence. Gene structure motif analyses showed that most have complex shared similar motifs. Homology modeling amino acid conservation revealed significant sequences, particularly Groups D E. Cis-acting element indicated respond to various stimuli, supported by expression under different stress conditions. Subcellular localization predictions proteins primarily localize nucleus cytoplasm. Alternative profile AS frequency likely varies between species. Functional AtU2AF65B mutant Arabidopsis loss enhances root elongation attenuates ABA-dependent germination suppression, negatively regulated seedling growth development. These findings provide insights history, molecular mechanisms, functional roles plants.

Language: Английский

Citations

0