Detection of Rocahepevirus ratti in Bivalve Mollusks from São Luís Island, Maranhão, Brazil: A Potential Transmission Route of an Emerging Zoonotic Pathogen?

Food and Environmental Virology, Год журнала: 2025, Номер 17(1)

Опубликована: Янв. 4, 2025

Язык: Английский

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Multifunctional ORF3 protein of hepatitis E virus

Journal of Medical Virology, Год журнала: 2024, Номер 96(6)

Опубликована: Май 24, 2024

Hepatitis E virus (HEV) is an emerging zoonotic pathogen that transmitted primarily through the fecal-oral route and can cause acute hepatitis in humans. Since HEV was identified as a pathogen, different species of strains have been globally from various hosts, leading to expanding range hosts. The genome consists 5' noncoding region, three open reading frames (ORFs), 3' region. ORF3 protein smallest but has many functions release pathogenesis. In this review, we systematically summarize recent progress understanding virion release, biogenesis quasi-enveloped viruses, antigenicity, host environmental regulation. This review will help us understand replication pathogenesis mechanisms better.

Язык: Английский

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Virome landscape of wild rodents and shrews in Central China

Microbiome, Год журнала: 2025, Номер 13(1)

Опубликована: Март 3, 2025

Wild rodents and shrews serve as vital sentinel species for monitoring zoonotic viruses due to their close interaction with human environments role natural reservoirs diverse viral pathogens. Although several studies have explored diversity assessed pathogenic risks in wild shrews, the full extent of this remains insufficiently understood. We conducted high-throughput sequencing on 1113 small mammals collected from 97 townships across seven cities Hubei Province during 2021, supplemented by publicly available data 2014 2016–2017. This analysis revealed a array novel spanning families, including Arenaviridae, Hepeviridae, Chuviridae, Paramyxoviridae, Arteriviridae, Nodaviridae, Rhabdoviridae, Dicistroviridae, Astroviridae, Picornaviridae. Phylogenetic genome structure characterization highlighted discovery these viruses, enhancing our understanding evolution. Key host such Chodsigoa smithii, Anourosorex squamipes, Niviventer niviventer, Apodemus agrarius were identified significant contributors circulation, making them crucial targets future surveillance. Additionally, central Plain was recognized critical geographic hub transmission, underscoring its importance controlling spread. Machine learning models employed assess potential revealing that families Coronaviridae, Hantaviridae, Lispiviridae, Nairoviridae, Picornaviridae, Picobirnaviridae possess high likelihood infecting humans. Notably, rodent-derived Rotavirus A, HTNV, SEOV displayed almost complete amino acid identity human-derived counterparts, indicating risk outbreaks. study provides comprehensive virome landscape Central China, highlighting roles specific regions transmission. By identifying key hotspots spread assessing discovered research enhances virus ecology factors driving disease emergence. The findings emphasize need targeted surveillance proactive strategies mitigate spillovers, contributing global public health preparedness.

Язык: Английский

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Cost‐Effectiveness Analysis of Hepatitis E Vaccination Strategies for Swine Workers

Transboundary and Emerging Diseases, Год журнала: 2025, Номер 2025(1)

Опубликована: Янв. 1, 2025

Hepatitis E virus (HEV) is endemic in China, with swine as the most common reservoir. It poses a zoonotic public health risk to workers. This study evaluated cost‐effectiveness of hepatitis vaccination for this high‐risk group China. A decision tree‐Markov model was utilized evaluate two strategies, without or following screening, workers aged 16–60 China from societal perspectives, compared no vaccination. We calculated HEV‐related cases and deaths averted, quality‐adjusted life years (QALYs) gained, incremental ratios (ICERs) willingness‐to‐pay (WTP) threshold GDP per capita. sensitivity analysis conducted. Additionally, we stimulated scenarios fully receiving 3‐dose schedule, partially 2‐dose schedule. Both strategies significantly reduced ICERs were estimated be USD 11,428.16 9830.71/QALY averted respectively, both lower than capita (USD 12,325.24, 2023). Furthermore, one‐way identified discount rate, utility asymptomatic cases, probability symptomatic infection crucial factors affecting ICER. Probabilistic (PSA) showed 47.5% 52.5% Notably, screening cost‐ineffective after age 40 at price 138.0/dose. schedules cost‐effective, regardless while schedule strategy. would optimal Vaccination starting an earlier vaccine prices can improve cost‐effectiveness. may recommended during outbreak achieve

Язык: Английский

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Seroprevalence survey of Hepatitis E Virus in Domestic Pigs in Guangdong, China

Animals, Год журнала: 2024, Номер 14(13), С. 1861 - 1861

Опубликована: Июнь 24, 2024

The Hepatitis E virus (HEV) causes acute and chronic is a global public health concern. HEV genotypes 3 (HEV-3) 4 (HEV-4) are common to humans animals, domestic pigs wild boars have been identified as the main reservoirs. However, limited information available on status of infection in pigs, particularly Guangdong Province, China. This study aimed investigate seroprevalence pig farms within Province. A total 1568 serum samples were collected from 25 tested for anti-HEV IgG antibodies. Enzyme-linked immunosorbent assay (ELISA) results revealed that 57.53% (902/1568) 24 (24/25, 96%) positive Year, season, region, age all linked risk factors Guangdong, with season region showing more significant impacts. high confirmed its circulation among presence this antibody indicates was or present farms, posing zoonotic transmission exposed workers pork organs consumption.

Язык: Английский

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Epidemiological assessment of hepatitis E virus infection among 1565 pregnant women in Siem Reap, Cambodia using an in‐house double antigen sandwich ELISA

Hepatology Research, Год журнала: 2024, Номер unknown

Опубликована: Апрель 4, 2024

Abstract Aim This study investigated hepatitis E virus (HEV) prevalence among pregnant women in Siem Reap, Cambodia, by developing a cost‐effective, user‐friendly in‐house enzyme‐linked immunosorbent assay (ELISA) for detecting total anti‐HEV immunoglobulins (Ig). Methods The ELISA was designed large‐scale screening resource‐limited settings. Its performance benchmarked against two commercial tests: the Anti‐HEV IgG EIA (Institute of Immunology, Co. Ltd) and RecomLine LIA (Mikrogen). demonstrated sensitivity 76% 71.4%, specificity 94.1% 98.6%, tests, respectively, with overall agreement rates 92.4% 94.3%. Results Among 1565 tested women, 11.6% were positive. Prevalence increased age, particularly aged 35–40 years over 40 years. No significant associations found education, number children, family size, or history blood transfusion surgery, except occupation head as public officer. Of positive 22.7% had IgM, indicating recent ongoing infection. Conclusion concluded that is viable option HEV regions limited resources due to its high accuracy cost‐effectiveness. It suitable studies health interventions areas where endemic poses risk women.

Язык: Английский

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Hepatitis E virus and SARS-CoV-2 co-infection in an immunocompromised patient: a case report

Diagnostic Microbiology and Infectious Disease, Год журнала: 2024, Номер 110(2), С. 116471 - 116471

Опубликована: Июль 27, 2024

Язык: Английский

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Clinical characteristics and risk factors of Hepatitis E virus infection in Zhejiang Province: a multicenter case–control study

Frontiers in Public Health, Год журнала: 2024, Номер 12

Опубликована: Июль 5, 2024

Introduction Hepatitis E (HE), caused by the virus (HEV), is a significant cause of acute viral hepatitis globally and major public health concern, particularly in specific high-prevalence areas China, which have diverse transmission routes regional differences. Identifying primary risk factors for HE essential to develop targeted interventions vulnerable populations. Methods This study employed 1:1 matched case–control methodology, using standardized questionnaire complemented medical records data validation. Results Among 442 cases 428 healthy controls, case group had higher prevalence fatigue (46.21%) loss appetite (43.84%) compared control group. Furthermore, liver function indicators were significantly group, with an average alanine aminotransferase (ALT) level 621.94 U/L aspartate (AST) 411.53 U/L. Severe patients predominantly male, increased ALT AST levels reaching 1443.81 862.31 respectively, along incidence (90%) (75%). Multifactorial analysis indicated that frequent dining out (OR = 2.553, 95%CI:1.686–3.868), poor hygiene conditions 3.889, 95%CI:1.399–10.807), comorbid chronic illnesses 2.275, 95%CI:1.616–3.202) infection; conversely, good practices protective against infection 0.698, 95%CI:0.521–0.934). Conclusion In conclusion, Zhejiang Province closely associated dietary habits environmental hygiene, individuals diseases or co-infections are at risk. highlights need education reduce among these

Язык: Английский

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Prognosis of Acute HEV Infection in Patients With Liver Cirrhosis: A Retrospective Study of 628 Chinese Patients

Journal of Viral Hepatitis, Год журнала: 2024, Номер unknown

Опубликована: Окт. 8, 2024

ABSTRACT Acute hepatitis E virus infection is a serious global health problem, which significant cause of morbidity and mortality. The aim the present study was to characterise clinical features therapeutic response patients with acute HEV identify risk factors for poor prognosis. In retrospective from 01 January 2014 Januray 2022, we collected baseline data all eligible (HEV) followed up via interviews medical records. We explored feature Chinese infection. follow‐up were used total, 628 (AHE) fulfilled inclusion criteria did not meet exclusion criteria. Among them, 452 males 176 females (M:F = 2.57:1). median age at diagnosis 57.0 years (interquartile range: 46–64 years). serum levels alanine aminotransferase (ALT), aspartate (AST), total bilirubin (TBIL) elevated in this cohort (642.3 U/L, 216.2 104.1 μmol/L, respectively). hospitalisation duration 16 days. Compared without other liver diseases, cirrhosis show lower ALT AST level, poorer coagulation indices higher MELD scores. According multivariate analysis, cirrhosis, high score, low albumin concentration found be independent predictors prognosis AHE. Our lager sample size validate that some demographic serological quite different between with/without CLDs. Liver predictor hepatitis.

Язык: Английский

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Contamination of Hepatitis E Virus in Pig Livers of Different Market Types Collected from Seven Provinces of China

Foodborne Pathogens and Disease, Год журнала: 2024, Номер unknown

Опубликована: Окт. 22, 2024

Foodborne transmission of the Hepatitis E virus (HEV) is becoming an important public health problem in China, but food associated with HEV route remains unclear. Pig liver among suspected products involved transmission. Our research aimed to survey contamination rate and genotype identification pig livers from different types markets selected provinces China. reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was used screen for livers, nest RT-PCR partial amplification opren reading frame (ORF) 2, followed by sequencing, phylogenetic analysis determine positive samples. A total 787 samples 7 were collected. The average 8.13% (64/787), Inner Mongolia (14.29%, 1/7) Hebei province 23/161) showed highest rate. There a significant difference (

Язык: Английский

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