Evaluating the Effect of Dietary Protein–Energy Ratios on Yak Intestinal Microbiota Using High-Throughput 16S rRNA Gene Sequencing

Veterinary Sciences, Год журнала: 2025, Номер 12(3), С. 208 - 208

Опубликована: Март 1, 2025

This study investigated the impact of varying dietary protein–energy ratios on intestinal microbiota composition in postpartum weaned female yak. For this study, forty yaks were divided into four groups and provided with different treatments (group FA: high-energy high-protein, FB: low-protein, FC: low-energy FD: control group, 48% alfalfa hay, oat grass, 4% premix) to investigate variations microflora profiles metabolic responses. Rectal fecal samples (n = 24 × 2) collected at day 15 30, from all groups, total DNA was extracted estimate microbial heterogeneity community structures by 16S rRNA sequencing focusing V3–V4 regions, using Illumina Nova Seq 6000 platform. The results revealed a 5,669,645 raw data sequences (3,189,115 2,480,530 respectively). Results showed that FA FB had enhanced protein metabolism diversity, which marked significant increase (p < 0.05) abundance Ruminococcus. Conversely, FD group low level diversity predominance Clostridium Proteobacteria, indicating dysbiosis stress. It concluded imbalanced diets (groups FC FD) upregulated stress-related pathways no favorable shifts, whereas, significantly supported involved amino acids carbohydrate beneficially shifted gut microbiota. These findings emphasize importance supplementation appropriate proportions energy feed promote optimal health functioning, particularly for inhabiting high-altitude is challenging environment.

Язык: Английский

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High Altitude Microbiome: Insight into Yak Gut Microbiota and its Nutritional and Functional Involvement for Food Systems

Trends in Food Science & Technology, Год журнала: 2025, Номер unknown, С. 104897 - 104897

Опубликована: Фев. 1, 2025

Язык: Английский

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Microbiome analysis reveals dynamic changes of gut microbiota in Guizhou horse and Dutch Warmblood horses

Frontiers in Microbiology, Год журнала: 2025, Номер 16

Опубликована: Март 12, 2025

In recent years, the importance of gut microbiota in digestive absorption, metabolism, and immunity has garnered increasing attention. China possess abundant horse breed resources, particularly Guizhou horses, which play vital roles local agriculture, tourism, transportation. Despite this, there is a lack comparative studies on native horses (GZH) imported Dutch Warmblood (WH). To address this gap, fecal samples were collected from both GZH WH, 16S rRNA high-throughput sequencing was utilized to analyze differences their microbiota. The results indicated that compared with GZH, abundance bacterial community WH significantly higher, whereas fungal lower. Furthermore, PCoA-based scatter plot analysis demonstrated distinct structure bacteria fungi between two breeds. While types share similar major phyla, significant observed numerous genera. Moreover, functional predictions communities suggested exhibit more robust system enhanced glycan biosynthesis metabolism capabilities. This first report WH. emphasize among various breeds offer valuable insights into composition different

Язык: Английский

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Analysis of fungal diversity in the gut feces of wild takin (Budorcas taxicolor)

Frontiers in Microbiology, Год журнала: 2024, Номер 15

Опубликована: Апрель 18, 2024

The composition of the intestinal microbiome correlates significantly with an animal's health status. Hence, this indicator is highly important and sensitive for protecting endangered animals. However, data regarding fungal diversity wild

Язык: Английский

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Comparative analysis of gut microbiota in free range and house fed yaks from Linzhou County

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Апрель 24, 2025

Язык: Английский

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Epidemiological Exploration of Cryptosporidium spp., Giardia intestinalis, Enterocytozoon bieneusi, and Blastocystis spp. in Yaks: Investigating Ecological and Zoonotic Dynamics in Lhasa, Xizang

Veterinary Sciences, Год журнала: 2025, Номер 12(5), С. 504 - 504

Опубликована: Май 20, 2025

The yak (Bos grunniens), prevalent at an altitude between 3000 and 5000 m above sea level, provides the local inhabitants with meat, milk, leather, fuel (dung), transport. However, intestinal zoonotic parasites seriously endanger its holistic well-being. prime concern of this study is to investigate prevalence four globally ubiquitous enteric protozoans, namely Cryptosporidium spp., G. intestinalis, Blastocystis E. bieneusi in yaks from different areas Lhasa, Xizang. In given study, 377 fecal samples various regions Lhasa were obtained, including 161 Linzhou County, 66 Dangxiong 150 Nimu County cattle farms. Molecular identification these protozoans was done after amplification using PCR sequencing PCR-positive samples, further phylogenetic analysis performed. results indicated that bieneusi, spp. farms 48.5, 22.9, 47.8, 90.7%; 65.2, 13.6, 72.7, 87.9% County; 56.0, 29.3, 58.0, 80.0%, respectively, County. provide a basic reference for preventing controlling

Язык: Английский

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Intestinal fungi biogeography, succession and its association with diarrhea in pigs

Journal of Animal Science and Biotechnology/Journal of animal science and biotechnology, Год журнала: 2025, Номер 16(1)

Опубликована: Июнь 3, 2025

The composition and relative abundances of intestinal microbiota are closely related to animal growth, development health. This study provides a comprehensive analysis the spatial distribution temporal dynamics fungi in pigs, with focus on fungal alterations associated diarrhea. Intestinal digesta from duodenum, jejunum, ileum, cecum, colon feces 8 finishing pigs (180 days old) were collected. Fecal samples also collected 18 across different growth stages, including lactation (3 d), nursery (26 d, 35 49 growing (120 d) d). Additionally, 32 diarrheal healthy piglets at 28 old. Fungal community profiling these was performed using internal transcribed spacer (ITS) sequencing. A total 9,224 amplicon sequence variants (ASVs) detected all 220 samples. diversity exhibited clear biogeographic patterns, significantly lower richness Shannon index ileum (P < 0.05) higher large intestine 0.05). structure varied segments, Kazachstania dominating Geotrichum duodenum jejunum. Across fecal increased after weaning. PCoA results revealed that significant changes (R = 0.7313, P 0.001), core taxa dominated by Diutina catenulata, Aspergillus restrictus Tahromyces munnarensis. In addition, comparing without diarrhea, than those Kazachstania, enriched Geotrichum, Piromyces piglets. shows distinct variation, greater intestine. shifts dynamically age, particularly around weaning transition. highlights specific diarrhea caused stress, offering new insights into interplay between gut pig

Язык: Английский

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Comparative analysis of intestinal microbiota composition between free-ranged captive yak populations in Nimu County

Frontiers in Cellular and Infection Microbiology, Год журнала: 2024, Номер 14

Опубликована: Июнь 25, 2024

The intestinal microbiota assumes a pivotal role in modulating host metabolism, immune responses, overall health, and additional physiological dimensions. structural functional characteristics of the may cause alterations within host’s body to certain extent. composition gut is associated with environmental factors, dietary habits, other pertinent conditions. investigation into yaks remained relatively underexplored. An examination yak holds promise elucidating complex relationship between microbial communities adaptive responses its environment. In this study, were selected from two distinct conditions: those raised sheds (NS, n=6) grazed Nimu County (NF, n=6). Fecal samples collected subsequently processed for analysis through 16S rDNA ITS sequencing methodologies. results revealed that different feeding styles result significant differences Alpha diversity fungi yaks, while captive was conserved. addition, appeared abundance microorganisms taxa, phylum Verrucomicrobiota significantly enriched group NF Firmicutes higher NS. At genus level, Akkermansia , Paenibacillus Roseburia Dorea UCG_012 Anaerovorax Marvinbryantia Desemzia Olsenella Kocuria Ornithinimicrobium Parvibacter NS (P&lt;0.05 or P&lt;0.01). There difference function groups. observed variations are likely influenced by methods conditions both inside outside pen. findings offer prospective insights enhancing breeding expansion industry.

Язык: Английский

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Effect of Isopropyl Ester of Hydroxy Analogue of Methionine on Rumen Microbiome, Active Enzymes, and Protein Metabolism Pathways of Yak

Fermentation, Год журнала: 2024, Номер 10(2), С. 94 - 94

Опубликована: Фев. 4, 2024

This study aims to investigate the effect of methionine analogue 2-hydroxy-4-methylthiobutanoic acid isopropyl ester (HMBi) on rumen microbial community, carbohydrate-active enzymes (CAZy), and protein metabolism pathways in yak. Twenty-four male Maiwa yaks (252.79 ± 15.95 kg) were selected randomly divided into groups that received basal diet alone, or a supplemented with different amounts HMBi (5 g, 10 g 15 g). At phylum level, group receiving 5 showed considerably higher relative abundance Lentisphaerae than other treatment (p < 0.05). The Actinobacteria decreased linearly increase supplemental levels Prevotella increased increasing level supplementation Butyrivibrio 0.05), Alistipes tended decrease = 0.084). addition had linear quadratic effects CAZy functional proteins yak Conclusively, these results indicated feeding is an excellent strategy enhance carbohydrate breakdown, improve structure function.

Язык: Английский

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Effect of Different Feeds on the Fungi Microbiome of Suffolk Crossed with Tibetan Sheep

Life, Год журнала: 2023, Номер 13(11), С. 2210 - 2210

Опубликована: Ноя. 14, 2023

The gut microbiome plays an important role in the metabolism, nutrient absorption and immunocompetency of animals. dynamics microbiota can be influenced by modulatory factors that involve nutrition, environment, health, diseases, etc. Few reports have been documented regarding effects different feeds on fungi Suffolk crossed with Tibetan sheep. A total 30 sheep (ST sheep) were selected for study randomly divided into five equal groups (n = 6): AZ, BZ, CZ, DZ EZ. Group AZ was fed alfalfa oat grass, whereas group BZ mixture concentrated feed, grass. Groups EZ feed #1, #2 #3, respectively. All experimental animals twice a day four months, rectum samples collected analysis. Results revealed 2,781,461 raw reads 2,333,239 clean achieved ST When compared (164), shared amplicon sequence variants (ASVs) between CZ (109), (113) (118) as well along fewer. Conspicuous phyla (8) genera (56) examined free-range AZ. Genera including Xeromyces, Kazachstania, Cordyceps, Rhodotorula, Pichia, Spor, found higher groups. results this provide new insights farmed plateau. We concluded differences altered their microbiota.

Язык: Английский

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