Elucidation of the mechanism of berberine against gastric mucosa injury in a rat model with chronic atrophic gastritis based on a combined strategy of multi-omics and molecular biology
Frontiers in Pharmacology,
Год журнала:
2025,
Номер
15
Опубликована: Янв. 6, 2025
Berberine
(BBR)
is
widely
used
to
treat
gastrointestinal
diseases.
However,
the
pharmacological
mechanism
of
action
BBR
in
anti-chronic
atrophic
gastritis
(CAG)
remains
unclear.
This
study
aimed
investigate
CAG
by
integration
molecular
biology
and
multi-omics
studies
strategy.
The
model
was
established
alternating
drinking
water
0.1%
ammonia
20
mmol/L
sodium
deoxycholate,
accompanied
an
irregular
diet.
Serum
biochemical
indices
including
PGI,
PGII,
GAS-17,
IL-6,
IL-1β,
TNF-α
were
analyzed.
HE
AB-PAS
staining
employed
assess
pathological
damage
gastric
tissue.
underlying
treatment
explored
via
network
pharmacology,
transcriptomics,
targeted
metabolomics
intestinal
flora
analysis.
Finally,
relevant
key
targets
pathway
verified.
results
showed
that
exerted
therapeutic
effects
improving
alleviating
inflammation
response,
maintaining
mucosal
barrier's
integrity
repairing
tissues.
Network
pharmacology
mainly
involved
inflammatory
apoptosis,
angiogenesis
metabolic
processes.
Furthermore,
234
different
expression
genes
identified
tissue
transcriptome,
which
biological
processes
such
as
cell
adhesion,
angiogenesis,
migration
lipids
metabolism
regulating
MAPK
signaling
pathway.
Metabolomics
125
differential
metabolites
also
identified,
while
pathways
D-glutamine
D-glutamate
metabolism,
tyrosine
etc.
Integrating
transcriptomics
analyses
indicated
directly
regulated
Carnitine
C3:0,
LPC
(0:0/20:3),
L-Glutamic
Acid
FFA
(15:0)
acting
on
SLC25A20,
PNLIPRP1,
PLA2G4C,
GSR,
GFPT2,
GCLM,
CTPS1,
ACSL1,
ACOT4
ACOT2.
16S
rRNA
sequencing
revealed
could
restore
balance
gut
microbiota
dysbiosis
significantly
relative
abundance
unclassified_Muribaculaceae
Lactobacillus_johnsonii.
demonstrated
alleviates
through
regulation
pathway,
disorders
dysbiosis,
thereby
revealing
complex
relation
from
multiple
levels
perspectives.
Язык: Английский
Effects of Yupingfeng polysaccharide in diet on slaughtering performance and meat flavor of Qingyuan partridge chicken
Food Chemistry,
Год журнала:
2025,
Номер
471, С. 142814 - 142814
Опубликована: Янв. 7, 2025
Язык: Английский
Dietary areca nut extract supplementation modulates the growth performance and immunity of Jiaji ducks (Cairina moschata)
Poultry Science,
Год журнала:
2025,
Номер
104(5), С. 104971 - 104971
Опубликована: Март 5, 2025
Areca
nut
extract
(ANE)
has
a
variety
of
pharmacological
effects
on
animals.
Here,
we
investigated
the
influence
ANE
slaughter
performance
and
immune
function
Jiaji
ducks.
One
hundred
fifty
42-day-old
healthy
ducks
were
randomly
divided
into
2
groups
(5
replicates
15
each),
named
DCK
group
(control)
DNT
(treatment),
respectively.
Ducks
in
fed
basal
diet
food
supplemented
with
0.08
g
per
kg
diet.
Additionally,
using
proteomics,
untargeted
metabolomics,
metagenomics,
analyzed
impact
protein
profile
spleen,
composition
plasma
metabolites,
structure
cecal
microbiota.
The
results
showed
that
dietary
inclusion
significantly
increased
rate
Proteomic
analysis
revealed
78
differentially
expressed
proteins
spleens
ANE-treated
birds,
including
54
up-regulated
24
down-regulated
group,
mainly
enriched
cell
adhesion
molecules
glutathione
metabolic
pathways.
Untargeted
metabolomic
117
serum
metabolites
regulated
between
groups;
meanwhile,
KEGG
pathway
indicated
these
involved
arachidonic
acid
metabolism,
phospholipase
D
signaling
eicosanoids.
Furthermore,
metagenomic
genus
Methanobrevibacter
was
downregulated
supplementation
group.
Combined,
analyses
relative
abundance
Prevotella
lower
than
negatively
correlated
levels
anti-inflammatory
compound
hydrocinnamic
lipid
metabolism
regulator
ganoderic
A.
This
study
provides
reference
for
application
as
supplement
Язык: Английский
Integrated metabolomics and intestinal microbiota analysis to reveal anti-post-weaning diarrhea mechanisms of Modified Yupingfeng Granule in Rex rabbits
Frontiers in Microbiology,
Год журнала:
2025,
Номер
16
Опубликована: Апрель 10, 2025
Introduction
Post-weaning
Diarrhea
(PWD)
is
a
kind
of
physiological
stress
diarrhea
in
Rex
rabbits
after
weaning,
which
can
lead
to
death
severe
cases.
Traditional
Chinese
medicine
(TCM)
has
been
widely
used
animal
due
its
advantages
natural
origin,
diverse
functions,
safety,
reliability,
economy
and
environmental
protection.
Modified
Yupingfeng
Granule
(MYPFG)
an
improved
prescription
based
on
the
famous
traditional
(YPF),
combined
with
other
TCM
obvious
synergistic
additive
activity
order
obtain
excellent
for
PWD.
Methods
In
this
study,
120
weaned
were
randomly
allocated
4
treatment
groups,
including
control
(CON),
low
dose
(LD),
medium
(MD),
high
(HD).
Rabbits
fed
diet
or
different
MYPFG
proportions
30
days.
The
study
16S
rRNA
analysis
intestinal
microbiota
cecal
contents
metabolomics
explore
effect
rabbits.
Results
increased
average
daily
gain,
villus
length
crypt
depth
ratio
decreased
feed
meat
ratio,
frequency,
mortality
rate,
(
p
<
0.05).
test
found
that
could
change
abundances
Patescibacteria,
Sphingobium
,
Ruminococcus,
Oxalobacter
.
Metabolomics
may
be
related
regulation
Glycine,
serine
threonine
metabolism,
Arginine
proline
metabolism.
Nicotinate
nicotinamide
Discussion
regulate
metabolic
pathway
some
amino
acids
alleviate
PWD
Язык: Английский
Qiju Dihuang Pill protects the lens epithelial cells via alleviating cuproptosis in diabetic cataract
Journal of Ethnopharmacology,
Год журнала:
2024,
Номер
333, С. 118444 - 118444
Опубликована: Июнь 6, 2024
Язык: Английский
Supplementation of Yupingfeng polysaccharides in low fishmeal diets enhances intestinal health through influencing the intestinal barrier, immunity, and microflora in Macrobrachium rosenbergii
Frontiers in Immunology,
Год журнала:
2024,
Номер
15
Опубликована: Ноя. 26, 2024
This
study
aimed
to
investigate
the
effects
of
a
low-fishmeal
diet
(LF,
substituting
soybean
meal
for
40%
fish
meal)
and
supplementation
500
mg/kg
1000
Yu
Ping
Feng
(YPF)
polysaccharides
on
growth
performance,
antioxidant
enzyme
activities,
intestinal
ultrastructure,
non-specific
immunity,
microbiota
Язык: Английский
Strategically using Yupingfeng polysaccharides to improve the growth performance, antioxidant capacity, liver health, and lipid metabolism of largemouth bass (Micropterus salmoides) under a high-lipid diet
Zhuozhi Ou,
Yuhua Yue,
Xianjun Feng
и другие.
Aquaculture Reports,
Год журнала:
2024,
Номер
39, С. 102473 - 102473
Опубликована: Ноя. 11, 2024
Язык: Английский
Effects of dietary Brassica rapa L. polysaccharide on growth performance, immune and antioxidant functions and intestinal flora of yellow-feathered quail
Scientific Reports,
Год журнала:
2024,
Номер
14(1)
Опубликована: Ноя. 16, 2024
This
study
aimed
to
explore
the
impact
of
Brassica
rapa
L.
polysaccharides
(BRP)
on
growth,
immune
response,
antioxidant
capacity,
and
cecal
microbiota
in
yellow-feathered
quails.
A
total
250
one-day-old
quails,
evenly
divided
by
sex,
were
randomly
assigned
five
groups,
with
each
group
comprising
ten
replicates
quails
each.
The
control
(CON)
received
a
basic
diet,
while
antibiotic
(CTC)
was
fed
diet
supplemented
chlortetracycline
(0.05
g/kg).
BRP
administered
at
concentrations
0.25
g/kg
(Low
dose
BRP,
LBRP),
0.5
(Medium
MBRP),
1
(High
HBRP).
duration
experiment
42
days.
results
indicated
that,
compared
CON
group,
final
body
weight
MBRP
significantly
increased
(P
<
0.05),
there
significant
difference
between
LBRP
CTC
0.05).
At
21
days
age,
average
weights
thymus
bursa
Fabricius
greater
than
those
no
observed
when
>
0.05);
that
levels
IgA
IgG
elevated
noted
Additionally,
showed
increases
CAT,
T-SOD,
GSH-Px
0.05)
group;
IL-1β
TNF-α
reduced
level
IL-10
group.
Furthermore,
16
S
rRNA
sequencing
revealed
supplementation
populations
beneficial
bacteria
such
as
Lactococcus,
Weissella,
Parabacteroides,
norank_f_Ruminococcaceae,
decreased
population
harmful
bacterium
Campylobacter,
indicating
modulates
microbial
community
structure
cecum
In
summary,
enhanced
growth
performance,
serum
immunoglobulin
levels,
functions,
improved
intestinal
Язык: Английский