Advancing environmental sustainability through microbial reprogramming in growth improvement, stress alleviation, and phytoremediation

Plant Stress, Год журнала: 2023, Номер 10, С. 100283 - 100283

Опубликована: Ноя. 7, 2023

The substantial influence of microbes on crop growth, stress resilience, and ecological restoration has generated considerable interest due to the intricate interplay between these microorganisms plants. This study comprehensively examines diverse mechanisms through which contribute plant well-being, mitigate stress, facilitate phytoremediation processes. Microorganisms encompassing bacteria, fungi, archaea, viruses, have demonstrated their knack for stirring up growth-enabling hormones, activating pathways tuned ameliorating availability nutrients by means fixation solubilization Furthermore, such also display immense potential in field strategies aiding plants extraction, alteration, detoxification contaminants found both soil water. Complementing this, enable phytoextraction, rhizofiltration, phytostabilization, rhizodegradation, owing harmonious interaction with purification tainted environments. However, it is critical address legal issues, moral dilemmas, unintended consequences as are increasingly incorporated into sustainable agriculture methods. Optimizing microbial therapies ensuring appropriate use offers promising insights when leveraging cutting-edge technologies like omics genetic engineering. Coordination among academics, practitioners, policymakers still crucial quest a more robust peaceful coexistence microbes, plants, ecosystems. In nutshell, this work highlights pivotal role that poised assume, guiding trajectory agriculture, alleviating fostering environmental sustainability far-reaching implications.

Язык: Английский

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Chalcone synthase (CHS) family genes regulate the growth and response of cucumber (Cucumis sativus L.) to Botrytis cinerea and abiotic stresses

Plant Stress, Год журнала: 2023, Номер 8, С. 100159 - 100159

Опубликована: Апрель 24, 2023

The chalcone synthase (CHS) gene family is instrumental not only regulating plant growth and development but fine-tuning response to environmental hazards. Despite the growing body of literature, role CHS genes in cucumber (Cucumis sativus L.) remains elusive. Herein, we identified 4 CsCHS from genome database. evolutionary relationship, structure, conserved motifs, proteins interaction chemical properties highlight key aspects cucumbers. Evolutionary tree analysis reveals that divided into four clades. Moreover, Gene Ontology (GO) showed regulate various stimuli. Microarray expression data were expressed almost all tested tissues. Additionally, differential patterns found under salinity stress hormonal treatments. qRT-PCR aphid infestation revealed they are components defense against insect pests. Under waterlogging stress, CsCHS3 displayed high naphthalene acetic acid (NAA) ethylene (ETH). dual luciferase assay indicated CsCHS2 effectively binds promoters markers CsERF1 CsERF3. Our results have provided detailed knowledge regarding gene's influential also providing resistive plants. We believe our study will facilitate breeding stress-resilient cultivars, particularly pests stress.

Язык: Английский

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The Homeodomain-Leucine Zipper Genes Family Regulates the Jinggangmycin Mediated Immune Response of Oryza sativa to Nilaparvata lugens, and Laodelphax striatellus

Bioengineering, Год журнала: 2022, Номер 9(8), С. 398 - 398

Опубликована: Авг. 17, 2022

The homeodomain-leucine zipper (HDZIP) is an important transcription factor family, instrumental not only in growth but finetuning plant responses to environmental adversaries. Despite the plethora of literature available, role HDZIP genes under chewing and sucking insects remains elusive. Herein, we identified 40 OsHDZIP from rice genome database. evolutionary relationship, gene structure, conserved motifs, chemical properties highlight key aspects rice. family divided into a further four subfamilies (i.e., I, II, III, IV). Moreover, protein-protein interaction Gene Ontology (GO) analysis showed that regulate response various stimuli. Various microRNA (miRNA) families targeted III subfamily genes. microarray data was expressed almost all tested tissues. Additionally, differential expression patterns were found salinity stress hormonal treatments, whereas brown planthopper (BPH), striped stem borer (SSB), leaf folder (RLF), OsHDZIP3, OsHDZIP4, OsHDZIP40, OsHDZIP10, OsHDZIP20 displayed expression. qRT-PCR validated OsHDZIP20, OsHDZIP10 BPH, small (SBPH) infestations, jinggangmycin (JGM) spraying applications. Our results provide detailed knowledge resistance plants will facilitate development stress-resilient cultivars, particularly against insect pests.

Язык: Английский

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Genome-wide identification, characterization, and expression analysis of the Chalcone Synthase gene family in Oryza sativa under Abiotic Stresses

Plant Stress, Год журнала: 2023, Номер 9, С. 100201 - 100201

Опубликована: Авг. 9, 2023

Chalcone synthase (CHS) is the pivotal enzyme that catalyzes first committed step of phenylpropanoid pathway leading to flavonoids. Herein, we identified twenty-seven OsCHS genes from rice genome database. The bioinformatics analysis, including conserved motif, domain, interactive proteins, gene ontology (GO), target micro RNAs (miRNA) prediction, and physiochemical properties, highlight key aspects in Oryza sativa L. proteins' phylogeny was further clustered into 4 sub-groups (Group I- Group IV) based on structural similarities functional diversities. GO analysis proteins showed they are highly stress-responsive (biotic/abiotic) involved various growth developmental processes. microarray expression transcription twelve tissues, differential pattern revealed their possible involvement against salinity drought. higher OsCHS15 OsWRKY65-OE line indicates its potential hormonal-mediated stress response. predicted miRNA includes miR2260, miR167, miR11337, miR2232families engagement posttranslational regulation stress-responsive. Owing above-mentioned outcomes, speculate O. play an essential role not only development but also fine-tuning immune responses environmental stresses provide major indications for studies.

Язык: Английский

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Jasmonic Acid (JA) Signaling Pathway in Rice Defense Against Chilo suppressalis Infestation

Rice, Год журнала: 2025, Номер 18(1)

Опубликована: Фев. 18, 2025

Jasmonic acid (JA) signaling plays a crucial role in rice defense against the striped stem borer, Chilo suppressalis, notorious pest causing significant yield losses. This review explores current understanding of JA-mediated mechanisms rice, focusing on molecular basis, regulatory elements, and practical implications for management. JA biosynthesis pathways are induced upon C. suppressalis infestation, leading to activation various responses. These include upregulation JA-responsive genes involved production proteinase inhibitors, volatile organic compounds, other defensive compounds. The also discusses crosstalk between hormonal pathways, such as salicylic ethylene, fine-tuning Structural modifications plants, cell wall reinforcement accumulation secondary metabolites, have been highlighted key components suppressalis. Furthermore, applications this knowledge breeding insect-resistant varieties developing sustainable management strategies were explored. Future research directions proposed further elucidate complexities rice-insect interactions harness enhance crop protection.

Язык: Английский

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Identification and Molecular Characterization of RWP-RK Transcription Factors in Soybean

Genes, Год журнала: 2023, Номер 14(2), С. 369 - 369

Опубликована: Янв. 31, 2023

The RWP-RK is a small family of plant-specific transcription factors that are mainly involved in nitrate starvation responses, gametogenesis, and root nodulation. To date, the molecular mechanisms underpinning nitrate-regulated gene expression many plant species have been extensively studied. However, regulation nodulation-specific NIN proteins during nodulation rhizobial infection under nitrogen soybean still remain unclear. Here, we investigated genome-wide identification their essential role nitrate-inducible stress-responsive soybean. In total, 28 genes were identified from genome, which unevenly distributed on 20 chromosomes 5 distinct groups phylogeny classification. conserved topology protein motifs, cis-acting elements, functional annotation has led to potential as key regulators growth, development, diverse stress responses. RNA-seq data revealed up-regulation GmRWP-RK nodules indicated these might play crucial roles Furthermore, qRT-PCR analysis most Phytophthora sojae environmental conditions (such heat, nitrogen, salt) significantly induced, thus opening new window possibilities into regulatory adaptation allow tolerate biotic abiotic stress. addition, dual luciferase assay GmRWP-RK1 GmRWP-RK2 efficiently bind promoters GmYUC2, GmSPL9, GmNIN, highlighting possible involvement nodule formation. Together, our findings provide novel insights defense responses

Язык: Английский

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Identification and expression profiles of Tubby‑like proteins coding genes in walnut (Juglans regia L.) in response to stress and hormone treatments

Plant Stress, Год журнала: 2024, Номер 12, С. 100472 - 100472

Опубликована: Апрель 30, 2024

Tubby-like proteins (TLPs) constitute a diverse multigene family found across broad range of eukaryotic organisms, from single-celled to multicellular eukaryotes. Despite their importance, the existence TLPs in walnuts (Juglans regia L.) has not been reported until now. In this study, we identified 12 members JrTLP gene walnut genome, revealing pattern replication events. Notably, all JrTLPs exhibited split structure, containing two eight introns, and these genes could be divided into five distinct categories based on evolutionary relationships. The majority TLP possess signature domains: F-box Tubby domains. Subcellular localization prediction experiments confirmed that JrTLP2 was located nucleus membrane. Expression profiling revealed multiple respond stimuli, including pathogen infection, hormonal treatment (such as auxin, ABA, MeJA, GA), abiotic stress conditions such cold, heat, salt. This study represents first comprehensive identification family, providing valuable insights function regulation laying foundation for future functional dissection, particularly context tolerance.

Язык: Английский

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Genome-wide analysis reveals the TCP-miR159-miR319 module is crucial for rice (Oryza sativa L.) growth and response to drought and salinity.

Plant Stress, Год журнала: 2023, Номер 10, С. 100215 - 100215

Опубликована: Сен. 6, 2023

The TCP are key plant-specific proteins responsible for regulating various developmental activities. Herein, we retrieved 21 OsTCP genes from the genome database of rice. bioinformatics analysis, including conserved motif, domain, interactive proteins, gene ontology (GO) target micro-RNAs (miRNA) prediction, and physiochemical properties, highlight aspects in Oryza sativa L. phylogeny analysis disclosed that was further clustered into five clades (Clade I - Clade V) based on structural similarities functional diversities. GO showed they primarily exercising rice processes, reproductive stages particular. microarray expression transcription twelve tissues, differential pattern revealed their possible involvement auxin-mediated growth response against salinity drought. predicted miRNA includes miR159 miR319 families engagement post-translational regulation growth. Owing to above-mentioned outcomes, speculate O. play an essential role development fine-tuning immune responses environmental stresses, thus providing major indications studies.

Язык: Английский

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Surviving a Double-Edged Sword: Response of Horticultural Crops to Multiple Abiotic Stressors

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(10), С. 5199 - 5199

Опубликована: Май 10, 2024

Climate change-induced weather events, such as extreme temperatures, prolonged drought spells, or flooding, pose an enormous risk to crop productivity. Studies on the implications of multiple stresses may vary from those a single stress. Usually, these coincide, amplifying extent collateral damage and contributing significant financial losses. The breadth investigations focusing response horticultural crops abiotic stress is immense. However, tolerance mechanisms remain poorly understood. In this review, we described most prevalent types that occur simultaneously discussed them in in-depth detail regarding physiological molecular responses crops. particular, transcriptional, posttranscriptional, metabolic stresses. Strategies breed multi-stress-resilient lines have been presented. Our manuscript presents interesting amount proposed knowledge could be valuable generating resilient genotypes for stressors.

Язык: Английский

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Genome-wide identification, characterization, and expression analysis of the Ovate family protein in Oryza sativa under biotic and abiotic stresses

Plant Stress, Год журнала: 2023, Номер 10, С. 100228 - 100228

Опубликована: Сен. 20, 2023

The plant-specific Ovate Family Proteins (OFPs) are transcription factors with a conserved OVATE domain. OFPs regulate organ shape in Arabidopsis and many crops, including rice, tomato, melon. However, little is known regarding the OFPs' potential role regulating plant immune response to biotic/abiotic stresses. Herein, we reported identification, bioinformatic, Insilco analysis of plant-specific, novel factor rice (Oryza sativa L). Thirty-one OsOFP genes were identified from genome database further divided into six subgroups based on their sequence similarities. Gene expression developmental stages indicated that some members gene family displayed tissue-specific patterns. Enrichment hormonal-related cis-acting elements varied under phytohormones validated participation hormone-mediated growth. microarray showed possible involvement against salinity, drought, brown planthopper (BPH)_ striped stemborer (SSB) (OsOFP25), leaf folder (RLF) (OsOFP26) infestations. higher OsOFP25 OsWRKY65-OE line implies key reproductive biology. These results provide systematic lay foundation for functional characterization this family.

Язык: Английский

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