Environmental Sciences Europe,
Год журнала:
2024,
Номер
36(1)
Опубликована: Окт. 22, 2024
Abstract
Background
Oilseed
plants
of
the
Brassicaceae
plant
family
are
cultivated
for
food,
feed
and
industrial
purposes
on
large-scale
in
Europe.
This
review
gives
an
overview
current
market-oriented
applications
new
genomic
techniques
(NGTs)
relevant
oilseed
crops
based
a
literature
survey.
In
this
respect,
changes
oil
quality,
yield,
growth
resistance
to
biotic
abiotic
stress
under
development
rape
(Brassica
napus),
camelina
(
Camelina
sativa
),
pennycress
Thlaspi
arvense
)
.
Main
findings
Environmental
risk
scenarios
starting
with
hazard
identification
developed
specific
NGT
either
changed
composition
or
fitness-related
traits.
case
composition,
increase
decrease
polyunsaturated
fatty
acids
(PUFA)
may
lead
risks
health
survival
pollinators.
Regarding
traits,
other
were
identified,
i.e.
increased
spread
persistence
plants.
Furthermore,
there
indications
potential
disturbance
interactions
environment,
involving
signalling
pathways
reaction
conditions.
Conclusion
It
is
shown
that
environmental
technological
specificities
NGTs,
plants’
biology
scale
releases
have
be
considered
combination.
Therefore,
release
into
environment
agricultural
will,
also
future,
require
assessment
monitoring
individual
traits
as
well
combinatorial
long-term
cumulative
effects.
addition,
management
should
develop
concepts
measures
control
potentially
limit
releases.
especially
Europe,
which
centre
diversity
family.
Plants,
Год журнала:
2024,
Номер
13(5), С. 578 - 578
Опубликована: Фев. 21, 2024
Rice
is
an
important
diet
source
for
the
majority
of
world’s
population,
and
meeting
growing
need
rice
requires
significant
improvements
at
production
level.
Hybrid
has
been
a
breakthrough
in
this
regard,
floral
traits
play
major
role
development
hybrid
rice.
In
grass
species,
structural
units
called
florets
spikelets
contains
different
floret
organs
such
as
lemma,
palea,
style
length,
anther,
stigma
exsertion.
These
are
crucial
enhancing
uplifting
cultivation
broader
Recent
advances
breeding
techniques
also
provide
knowledge
about
how
they
can
be
improved
by
using
biotechnological
better
The
flower
holds
immense
significance
primary
focal
point
researchers
working
on
molecular
biology.
Furthermore,
unique
genetics
maintaining
its
structure.
However,
to
improve
varieties
further,
we
identify
genomic
regions
through
mapping
QTLs
(quantitative
trait
loci)
or
GWAS
(genome-wide
association
studies)
their
validation
should
performed
developing
user-friendly
markers,
Kompetitive
allele-specific
PCR
(KASP).
This
review
outlines
benefits
modern
approaches
production.
It
focuses
interrelated
possible
contribution
satisfy
future
demand.
We
discuss
traits,
techniques,
historical
perspective
current
status
outline
challenges
opportunities
field.
Plant Biotechnology Journal,
Год журнала:
2024,
Номер
22(10), С. 2773 - 2784
Опубликована: Июнь 10, 2024
Camelina
(Camelina
sativa
L.),
a
hexaploid
member
of
the
Brassicaceae
family,
is
an
emerging
oilseed
crop
being
developed
to
meet
increasing
demand
for
plant
oils
as
biofuel
feedstocks.
In
other
Brassicas,
high
oil
content
can
be
associated
with
yellow
seed
phenotype,
which
unknown
camelina.
We
sought
create
camelina
using
CRISPR/Cas9
technology
disrupt
its
Transparent
Testa
8
(TT8)
transcription
factor
genes
and
evaluate
resulting
phenotype.
identified
three
TT8
genes,
one
in
each
subgenomes,
obtained
independent
CsTT8
lines
containing
frameshift
edits.
Disruption
caused
coat
colour
change
from
brown
reflecting
their
reduced
flavonoid
accumulation
up
44%,
loss
well-organized
mucilage
layer.
Transcriptomic
analysis
CsTT8-edited
seeds
revealed
significantly
increased
expression
lipid-related
factors
LEC1,
LEC2,
FUS3,
WRI1
downstream
fatty
acid
synthesis-related
targets.
These
changes
metabolic
remodelling
synthesis
rates
corresponding
increases
total
(TFA)
32.4%
38.0%
weight,
TAG
yield
by
more
than
21%
without
significant
starch
or
protein
levels
compared
parental
line.
data
highlight
effectiveness
CRISPR
creating
novel
enhanced-oil
germplasm
The
may
directly
contribute
future
net-zero
carbon
energy
production
combined
traits
produce
desired
lipid-derived
bioproducts
at
yields.
PLANT PHYSIOLOGY,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 15, 2025
Abstract
The
emerging
crop
Camelina
sativa
(L.)
Crantz
(camelina)
is
a
Brassicaceae
oilseed
with
rapidly
growing
reputation
for
the
deployment
of
advanced
lipid
biotechnology
and
metabolic
engineering.
recognised
by
agronomists
its
traits
including
yield,
oil/protein
content,
drought
tolerance,
limited
input
requirements,
plasticity
resilience.
Its
utility
as
platform
engineering
was
then
quickly
recognised,
biotechnologists
have
benefited
from
short
life
cycle
facile
genetic
transformation,
producing
numerous
transgenic
interventions
to
modify
seed
content
generate
novel
products.
desire
work
plant
that
both
model
has
driven
expansion
research
resources
camelina,
increased
availability
genome
other
“-omics”
data
sets.
Collectively
these
established
camelina
an
ideal
study
regulation
metabolism
improvement.
Furthermore,
unique
characteristics
enables
design-build-test-learn
be
transitioned
controlled
environment
field.
Complex
synthesize
accumulate
high
levels
fatty
acids
modified
oils
in
seeds,
can
deployed,
tested
undergo
rounds
iteration
agronomically
relevant
environments.
Engineered
are
now
increasingly
being
developed
used
sustainably
supply,
improved
nutrition,
feed,
biofuels
fossil
fuel
replacements
high-value
chemical
In
this
review,
we
provide
summary
acid
synthesis
oil
assembly
highlighting
how
discovery
supports
advance
towards
predictive
manipulation
produce
desirable
bio-based
Further
examples
innovation
improvement
provided,
describing
technologies
(e.g.,
modification
(GM),
gene
editing
(GE),
RNAi,
alongside
GM
GE
stacking)
applied
new
products
denude
undesirable
traits.
Focusing
on
production
long
chain
polyunsaturated
omega-3
describe
transition
commercial
prototype.
prospects
structured
triacylglycerol
specified
stereospecific
positions
also
discussed,
future
outlook
agronomic
uptake
biotechnology.
Plant Biotechnology Journal,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 27, 2025
Summary
Mutants
with
simultaneous
germline
mutations
were
obtained
in
all
three
F5H
genes
and
FAD2
(one
to
eleven
mutated
alleles)
order
improve
the
feed
value
of
seed
meal
fatty
acid
composition
oil.
In
mutants
multiple
alleles,
sinapine
was
reduced
by
up
100%,
accompanied
a
sharp
reduction
S‐monolignol
content
lignin
without
causing
lodging
or
stem
break.
A
lower
S‐lignin
monomer
stems
can
contribute
improved
degradability
allowing
new
uses
stems.
six
alleles
showed
an
expected
increase
MUFA
from
8.7%
74%
PUFA
53%
13%
acids
Remarkably,
some
full
normal
growth
production
not
dwarfing
phenotype
reported
previous
studies.
The
relation
between
mutation
allele
dosage
influenced
still
ongoing
activity
CRISPR/Cas9
system,
leading
somatic
leaves
flowering
plants.
correlations
total
frequency
(germline
plus
mutations)
for
content,
higher
than
count
phenotypes.
This
shows
importance
quantifying
both
when
studying
effects
situations
where
system
is
yet
segregated
out.
Oilseed
plants
of
the
Brassicaceae
plant
family
are
cultivated
for
food,
feed
and
industrial
purposes
on
large-scale
in
Europe.
This
review
gives
an
overview
current
market-oriented
applications
new
genomic
techniques
(NGTs)
relevant
oilseed
crops
based
a
literature
survey.
In
this
respect,
changes
oil
quality,
yield,
growth
resistance
to
biotic
abiotic
stress
goals
rape
(B.
napus)
camelina
(C.
sativa).
Environmental
risk
assessment
scenarios
developed
specific
NGT
with
either
changed
composition
or
fitness
related
traits.
case
increase
decrease
polyunsaturated
fatty
acids
(PUFA)
demonstrates
risks
health
survival
pollinators.
traits
other
were
identified,
i.
e.
increased
spread
persistence
plants.
Furthermore,
there
indications
potential
disturbance
interactions
environment,
involving
signalling
pathways
reaction
conditions.
It
is
shown
that
comprehensive
technological
NGTs,
plants’
biology
scale
releases
have
be
considered
combination.
Therefore,
release
into
environment
agricultural
will
require
monitoring
single
as
well
combinatorial
long-term
cumulative
effects.
addition,
management
should
develop
concepts
measures
control
potentially
restrict
releases.
especially
Europe
which
centre
diversity
family.
Frontiers in Genome Editing,
Год журнала:
2024,
Номер
6
Опубликована: Дек. 5, 2024
Genome
editing
and
plant
transformation
are
crucial
techniques
in
biotechnology,
allowing
for
the
precise
modification
of
genomes
to
enhance
agronomically
essential
traits.
The
advancement
CRISPR-based
genome
tools
plants
is
limited,
among
others,
by
developing
novel
Plants,
Год журнала:
2023,
Номер
12(23), С. 4020 - 4020
Опубликована: Ноя. 29, 2023
Flowering
is
a
crucial
process
in
the
life
cycle
of
most
plants
as
it
essential
for
reproductive
success
and
genetic
diversity
species.
There
are
situations
which
breeders
want
to
expedite,
delay,
or
prevent
flowering,
example,
shorten
prolong
vegetative
growth,
unwanted
pollination,
reduce
risk
diseases
pests,
modify
plant's
phenotypes.
This
review
aims
provide
an
overview
current
state
knowledge
use
CRISPR/Cas9,
powerful
genome-editing
technology
specific
DNA
sequences
related
flowering
induction.
We
discuss
underlying
molecular
mechanisms
governing
regulation
photoperiod,
autonomous,
vernalization,
hormonal,
sugar,
aging,
temperature
signal
pathways
regulating
time.
In
addition,
we
investigating
effective
strategies
nominating
target
genes.
Furthermore,
have
collected
dataset
showing
successful
applications
CRISPR
accelerate
several
plant
species
from
2015
up
date.
Finally,
explore
opportunities
challenges
using
potential
time
engineering.
Oilseed
plants
of
the
Brassicaceae
plant
family
are
cultivated
for
food,
feed
and
industrial
purposes
on
large-scale
in
Europe.
This
review
gives
an
overview
current
market-oriented
applications
new
genomic
techniques
(NGTs)
relevant
oilseed
crops
based
a
literature
survey.
In
this
respect,
changes
oil
quality,
yield,
growth
resistance
to
biotic
abiotic
stress
goals
rape
(B.
napus)
camelina
(C.
sativa).
Environmental
risk
assessment
scenarios
developed
specific
NGT
with
either
changed
composition
or
fitness
related
traits.
case
increase
decrease
polyunsaturated
fatty
acids
(PUFA)
demonstrates
risks
health
survival
pollinators.
traits
other
were
identified,
i.
e.
increased
spread
persistence
plants.
Furthermore,
there
indications
potential
disturbance
interactions
environment,
involving
signalling
pathways
reaction
conditions.
It
is
shown
that
comprehensive
technological
NGTs,
plants’
biology
scale
releases
have
be
considered
combination.
Therefore,
release
into
environment
agricultural
will
require
monitoring
single
as
well
combinatorial
long-term
cumulative
effects.
addition,
management
should
develop
concepts
measures
control
potentially
restrict
releases.
especially
Europe
which
centre
diversity
family.
