Antagonism in Orthotospoviruses Is Reflected in Plant Small RNA Profile DOI Creative Commons
Md Tariqul Islam, Kaixi Zhao, Nathan Johnson

и другие.

Viruses, Год журнала: 2025, Номер 17(6), С. 789 - 789

Опубликована: Май 30, 2025

Mixed infections of plant viruses are commonly found in natural patho-systems and present a valuable opportunity to understand how multiple can co-infect the same host. Tomato spotted wilt orthotospovirus (TSWV) impatiens necrotic spot (INSV) geographic areas closely related. More mixed TSWV INSV have been reported recent years, host range has be increasing. In previous study, we isolated characterized one strain that they an antagonistic relationship their vectors. However, were unable determine whether this antagonism extends or uncover underlying mechanisms host’s contribution. Here, show exhibit interactions plant, as evidenced by lower viral titer compared single infections. Using small RNA sequencing, identified contributes through differential processing, which appears regulate replication success infection. This research advances our understanding virus–virus virus-host presents opportunities for leveraging these dynamics integrated pest management strategies.

Язык: Английский

Elucidating insect-virus associations in black pepper: Mealybug and tending ant influences on viral incidence in East Malaysia, Borneo DOI

Marthiuz Ghany Buang,

Ameyra Aman‐Zuki,

Nur Azura Adam

и другие.

Journal of Asia-Pacific Entomology, Год журнала: 2025, Номер unknown, С. 102426 - 102426

Опубликована: Май 1, 2025

Язык: Английский

Процитировано

0

Virus-Induced Genome Editing (VIGE): One Step Away from an Agricultural Revolution DOI Open Access
E. V. Mikhaylova

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(10), С. 4599 - 4599

Опубликована: Май 11, 2025

There is currently a worldwide trend towards deregulating the use of genome-edited plants. Virus-induced genome editing (VIGE) novel technique that utilizes viral vectors to transiently deliver clustered regularly interspaced short palindromic repeat (CRISPR) components into plant cells. It potentially allows us obtain transgene-free events in any species single generation without vitro tissue culture. This technology has great potential for agriculture and already being applied more than 14 using 20 viruses. The main limitations VIGE include insufficient vector capacity, unstable expression CRISPR-associated (Cas) protein, immune reaction, host specificity, reduced activity meristem. Various solutions these problems have been proposed, such as fusion mobile elements, RNAi suppressors, miniature Cas proteins, seed-borne viruses, but final goal not yet achieved. In this review, mechanism underlying ability different classes viruses edit genomes explained. only focuses on latest achievements virus-induced crops also provides suggestions improving technology. review may serve source new ideas those planning develop approaches VIGE.

Язык: Английский

Процитировано

0

Viral tropism in plants, reproductive tissues, and seeds DOI Creative Commons
Leandro Alberto Núñez-Muñoz, Berenice Calderón-Pérez, Roberto Ruíz‐Medrano

и другие.

Archives of Microbiology, Год журнала: 2025, Номер 207(7)

Опубликована: Май 23, 2025

Abstract Plant viral tropism refers to virus ability for infecting and replicating within specific cell types, tissues or hosts. is shaped by the absence of membrane-associated receptors supracellular nature transport through plasmodesmata vascular tissues. This review focuses on molecular cellular determinants plant tropism, including modifications in plasmodesmal permeability, host-mediated RNA silencing, tissue-specific protein localization. We discuss how certain viruses target reproductive organs, meristems, seeds, overcoming antiviral barriers establish persistent infections. Additionally, we explore role host factors shaping distribution. Advances super-resolution microscopy, single-cell transcriptomics, proteomics have significantly expanded our dissect virus-host interactions at nanoscale, uncovering new mechanisms accumulation. Understanding these processes essential not only improving crop resistance designing integrated disease management strategies, but also repurposing as tools targeted delivery biotechnological applications.

Язык: Английский

Процитировано

0

Antagonism in Orthotospoviruses Is Reflected in Plant Small RNA Profile DOI Creative Commons
Md Tariqul Islam, Kaixi Zhao, Nathan Johnson

и другие.

Viruses, Год журнала: 2025, Номер 17(6), С. 789 - 789

Опубликована: Май 30, 2025

Mixed infections of plant viruses are commonly found in natural patho-systems and present a valuable opportunity to understand how multiple can co-infect the same host. Tomato spotted wilt orthotospovirus (TSWV) impatiens necrotic spot (INSV) geographic areas closely related. More mixed TSWV INSV have been reported recent years, host range has be increasing. In previous study, we isolated characterized one strain that they an antagonistic relationship their vectors. However, were unable determine whether this antagonism extends or uncover underlying mechanisms host’s contribution. Here, show exhibit interactions plant, as evidenced by lower viral titer compared single infections. Using small RNA sequencing, identified contributes through differential processing, which appears regulate replication success infection. This research advances our understanding virus–virus virus-host presents opportunities for leveraging these dynamics integrated pest management strategies.

Язык: Английский

Процитировано

0