International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(10), P. 4599 - 4599
Published: May 11, 2025
There is currently a worldwide trend towards deregulating the use of genome-edited plants. Virus-induced genome editing (VIGE) novel technique that utilizes viral vectors to transiently deliver clustered regularly interspaced short palindromic repeat (CRISPR) components into plant cells. It potentially allows us obtain transgene-free events in any species single generation without vitro tissue culture. This technology has great potential for agriculture and already being applied more than 14 using 20 viruses. The main limitations VIGE include insufficient vector capacity, unstable expression CRISPR-associated (Cas) protein, immune reaction, host specificity, reduced activity meristem. Various solutions these problems have been proposed, such as fusion mobile elements, RNAi suppressors, miniature Cas proteins, seed-borne viruses, but final goal not yet achieved. In this review, mechanism underlying ability different classes viruses edit genomes explained. only focuses on latest achievements virus-induced crops also provides suggestions improving technology. review may serve source new ideas those planning develop approaches VIGE.
Language: Английский