Evolution of dirofilariasis diagnostic techniques from traditional morphological analysis to molecular-based techniques: a comprehensive review DOI Creative Commons
A. M. M. T. B. Aththanayaka,

B. S. W. M. T. B. Dayananda,

Koshila Ranasinghe

et al.

Frontiers in Parasitology, Journal Year: 2024, Volume and Issue: 3

Published: Aug. 27, 2024

Dirofilariasis, caused by the nematode Dirofilaria spp., poses significant challenges in diagnosis due to its diverse clinical manifestations and complex life cycle. This comprehensive literature review focuses on evolution of diagnostic methodologies, spanning from traditional morphological analyses modern emerging techniques context dirofilariasis diagnosis. The traces historical progression modalities, encompassing approaches such as microscopic examination, serological tests (including ELISA IFA), radiographic imaging, ultrasonography, necropsy, which laid foundation for subsequent advancements. integration molecular diagnostics marks a turning point with adoption polymerase chain reaction (PCR) assays real-time PCR (qPCR) facilitating enhanced sensitivity specificity. Furthermore, recent strides next-generation sequencing (NGS) technologies, including whole–genome (WGS), targeted (TS), metagenomic (MS), RNA (transcriptome sequencing), have revolutionized landscape diagnostics. Emerging loop-mediated isothermal amplification (LAMP), digital (dPCR), microfluidics are also explored their potential augment accuracy. addresses associated standardizing protocols, tackling false positives/negatives, discusses advantages limitations each technique. By providing overview strategies, cutting-edge methods, this aims enhance understanding disease’s landscape. insights gained implications improved disease management guide future research endeavors toward refining protocols advancing therapeutic interventions.

Language: Английский

Isolation, Identification, and Antimicrobial Susceptibilities of Bacteria from the Conjunctival Sacs of Dogs with Bacterial Conjunctivitis in Different Regions of Wuhan, China DOI Creative Commons

Yuxin Li,

Yinan Wang, Xin Gao

et al.

Veterinary Sciences, Journal Year: 2025, Volume and Issue: 12(1), P. 21 - 21

Published: Jan. 6, 2025

In order to investigate the bacterial species present in conjunctival sacs of dogs with conjunctivitis Wuhan (Hongshan District, Wuchang Jiangxia and Huangpi District) their resistance aminoglycoside antibiotics, samples sac secretions were collected from 56 various regions Wuhan. Drug susceptibility testing for antibiotics was performed on most commonly isolated gram-positive gram-negative bacteria. The expression two modifying enzyme genes, aacA-aphD aac (6')-Ib, three 16S rRNA methyltransferase rmtB, rmtE npmA, analyzed by PCR. results showed that a total 123 strains cultured secretion samples, Staphylococcus being species, followed Escherichia. Among them, 14 pseudointermedius not resistant tobramycin, amikacin, gentamicin or neomycin, but rates streptomycin kanamycin 35.71% 42.86%, respectively. Escherichia coli tobramycin gentamicin, they high neomycin (both at 50%). detection rate gene 100%. rmtB 85.71% 28.57%, respectively, while aac(6')-Ib npmA detected.

Language: Английский

Citations

0
Pioneering Advances in Veterinary Medicine: From Diagnosis to Treatment DOI Creative Commons
Felisbina L. Queiroga

Animals, Journal Year: 2025, Volume and Issue: 15(4), P. 516 - 516

Published: Feb. 12, 2025

Continuous advancements in veterinary medicine have significantly improved the health and longevity of companion animals while also contributing to wildlife conservation efforts [...]

Language: Английский

Citations

0
Correction: Domrazek, K.; Jurka, P. Application of Next-Generation Sequencing (NGS) Techniques for Selected Companion Animals. Animals 2024, 14, 1578 DOI Creative Commons
Kinga Domrazek, P. Jurka

Animals, Journal Year: 2025, Volume and Issue: 15(4), P. 606 - 606

Published: Feb. 19, 2025

The authors would like to make the following corrections in original publication [...].

Language: Английский

Citations

0
Detection of Mycobacteria in Arabian camels and antimycobacterial potential of Moringa oleifera DOI Creative Commons
Sahar A. Allam,

Eman Mahrous,

Sahar Tolba

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: April 4, 2025

Language: Английский

Citations

0
Evolution of dirofilariasis diagnostic techniques from traditional morphological analysis to molecular-based techniques: a comprehensive review DOI Creative Commons
A. M. M. T. B. Aththanayaka,

B. S. W. M. T. B. Dayananda,

Koshila Ranasinghe

et al.

Frontiers in Parasitology, Journal Year: 2024, Volume and Issue: 3

Published: Aug. 27, 2024

Dirofilariasis, caused by the nematode Dirofilaria spp., poses significant challenges in diagnosis due to its diverse clinical manifestations and complex life cycle. This comprehensive literature review focuses on evolution of diagnostic methodologies, spanning from traditional morphological analyses modern emerging techniques context dirofilariasis diagnosis. The traces historical progression modalities, encompassing approaches such as microscopic examination, serological tests (including ELISA IFA), radiographic imaging, ultrasonography, necropsy, which laid foundation for subsequent advancements. integration molecular diagnostics marks a turning point with adoption polymerase chain reaction (PCR) assays real-time PCR (qPCR) facilitating enhanced sensitivity specificity. Furthermore, recent strides next-generation sequencing (NGS) technologies, including whole–genome (WGS), targeted (TS), metagenomic (MS), RNA (transcriptome sequencing), have revolutionized landscape diagnostics. Emerging loop-mediated isothermal amplification (LAMP), digital (dPCR), microfluidics are also explored their potential augment accuracy. addresses associated standardizing protocols, tackling false positives/negatives, discusses advantages limitations each technique. By providing overview strategies, cutting-edge methods, this aims enhance understanding disease’s landscape. insights gained implications improved disease management guide future research endeavors toward refining protocols advancing therapeutic interventions.

Language: Английский

Citations

0