Biomarkers of oxidative stress and inflammation in subjects with COVID-19: Characterization and prognosis of the disease

Microbial Pathogenesis, Год журнала: 2023, Номер 184, С. 106339 - 106339

Опубликована: Сен. 9, 2023

Язык: Английский

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A review: Mechanism and prospect of gastrodin in prevention and treatment of T2DM and COVID-19

Heliyon, Год журнала: 2023, Номер 9(11), С. e21218 - e21218

Опубликована: Окт. 27, 2023

Gastrodin is an extract from the dried tuber of Chinese herb Gastrodia elata (Tian ma), with anti-inflammatory, antioxidant, and antiviral properties. Recent studies have shown that, compared to commonly used diabetes drugs, gastrodin has antidiabetic effects in multiple ways, characteristics low cost, high safety, less side effects, protection β-cell function, relieving insulin resistance alleviating complications. In addition, it confirmed that can protect function lung other organs, enhance activity via upregulating type I interferon (IFN–I), inhibit angiotensin II (AngII), a key factor "cytokine storm" caused by COVID-19. Therefore, we reviewed effect mechanism on 2 mellitus (T2DM), speculated potential mechanisms signal pathway, inflammation, mitochondrial endoplasmic reticulum its prevention treatment We hope provide new direction strategy for basic research clinical work: considered as drug

Язык: Английский

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Innate Immune Activation and Mitochondrial ROS Invoke Persistent Cardiac Conduction System Dysfunction after COVID-19

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Янв. 8, 2024

Background: Cardiac risk rises during acute SARS-CoV-2 infection and in long COVID syndrome humans, but the mechanisms behind COVID-19-linked arrhythmias are unknown. This study explores term effects of on cardiac conduction system (CCS) a hamster model COVID-19. Methods: Radiotelemetry conscious animals was used to non-invasively record electrocardiograms subpleural pressures after intranasal infection. cytokines, interferon-stimulated gene expression, macrophage infiltration CCS, were assessed at 4 days weeks post-infection. A double-stranded RNA mimetic, polyinosinic:polycytidylic acid (PIC), vivo vitro activate viral pattern recognition receptors absence Results: COVID-19 induced pronounced tachypnea severe dysfunction, spanning from bradycardia persistent atrioventricular block, although no protein expression detected heart. Arrhythmias developed rapidly, partially reversed, then redeveloped pulmonary resolved, indicating CCS injury. Increased remodeling accompanied electrophysiological abnormalities. Interestingly, arrhythmia phenotype reproduced by injection PIC virus, that innate immune activation sufficient drive response. also strongly cytokine secretion robust interferon signaling hearts, human iPSC-derived cardiomyocytes (hiPSC-CMs), engineered heart tissues, alterations electrical Ca2+ handling properties. Importantly, blunted inhibition JAK/STAT or mitochondrially-targeted antioxidant. Conclusions: The findings indicate dysfunction cell is COVID-19, arising indirectly oxidative stress excessive responses infection, with implications for Syndrome.

Язык: Английский

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Effect of Vaccination on Platelet Mitochondrial Bioenergy Function of Patients with Post-Acute COVID-19

Viruses, Год журнала: 2023, Номер 15(5), С. 1085 - 1085

Опубликована: Апрель 28, 2023

Background: Mitochondrial dysfunction and redox cellular imbalance indicate crucial function in COVID-19 pathogenesis. Since 11 March 2020, a global pandemic, health crisis economic disruption has been caused by SARS-CoV-2 virus. Vaccination is considered one of the most effective strategies for preventing viral infection. We tested hypothesis that preventive vaccination affects reduced bioenergetics platelet mitochondria biosynthesis endogenous coenzyme Q10 (CoQ10) patients with post-acute COVID-19. Material Methods: 10 vaccinated (V + PAC19) unvaccinated (PAC19) were included study. The control group (C) consisted 16 healthy volunteers. Platelet mitochondrial bioenergy was determined HRR method. CoQ10, γ-tocopherol, α-tocopherol β-carotene HPLC, TBARS (thiobarbituric acid reactive substances) spectrophotometrically. Results: protected but not CoQ10 levels, Conclusions: against virus infection prevented reduction respiration energy production. mechanism suppression levels fully known. Methods determination can be used monitoring targeted therapy

Язык: Английский

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Postbiotic Preparation of Lacticaseibacillus rhamnosus GG against Diarrhea and Oxidative Stress Induced by Spike Protein of SARS-CoV-2 in Human Enterocytes

Antioxidants, Год журнала: 2023, Номер 12(10), С. 1878 - 1878

Опубликована: Окт. 19, 2023

The Spike protein of SARS-CoV-2 acts as an enterotoxin able to induce chloride secretion and production reactive oxygen species (ROS), involved in diarrhea pathogenesis. L. rhamnosus GG (LGG) is recommended pediatric acute gastroenteritis guidelines a therapy independent infectious etiology. We tested postbiotic preparation LGG (mLGG) vitro model COVID-associated diarrhea. Caco-2 cell monolayers mounted Ussing chambers were exposed protein, electrical parameters secretory effect (Isc TEER) recorded the system. Oxidative stress was analyzed by measuring ROS (DCFH-DA), GSH levels (DNTB), lipid peroxidation (TBARS). Experiments repeated after mLGG pretreatment cells. Isc increase induced consistent with pattern, which dependent on oxidative defined 2-fold variation glutathione levels. significantly reduced (p = 0.002) stress, namely < 0.001), level changes 0.001). counteracts Spike-induced inhibiting enterotoxic stress. efficacy form depends metabolites secreted medium antioxidant properties similar NAC. Because enteric pathogen, etiology treatment confirmed our data.

Язык: Английский

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Association between Glutathione S-Transferases Gene Variants and COVID-19 Severity in Previously Vaccinated and Unvaccinated Polish Patients with Confirmed SARS-CoV-2 Infection

International Journal of Environmental Research and Public Health, Год журнала: 2023, Номер 20(4), С. 3752 - 3752

Опубликована: Фев. 20, 2023

As the outcome of COVID-19 is associated with oxidative stress, it highly probable that polymorphisms genes related to stress were susceptibility and severity COVID-19. The aim study was assess association glutathione S-transferases (GSTs) gene in previously vaccinated unvaccinated Polish patients confirmed SARS-CoV-2 infection. A total 92 not 84 hospitalized due included. WHO Clinical Progression Scale used severity. GSTs genetic assessed by appropriate PCR methods. Univariable multivariable analyses performed, including logistic regression analysis. GSTP1 Ile/Val genotype found be a higher risk developing severe form disease population (OR: 2.75; p = 0.0398). No significant observed for any GST genotypes In this group patients, BMI > 25 serum glucose level 99 mg% statistically significantly increased odds towards more Our results may contribute further understanding factors selecting need strategies focusing on stress.

Язык: Английский

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Selenium Status and Oxidative Stress in SARS-CoV-2 Patients

Medicina, Год журнала: 2023, Номер 59(3), С. 527 - 527

Опубликована: Март 8, 2023

Background and Objectives: Insufficient intake of essential micronutrient selenium (Se) increases the susceptibility to diseases associated with oxidative stress. The study aim was assess Se status stress in COVID-19 patients depending on severity disease. Materials Methods: Blood plasma 80 post-COVID-19 disease 40 acutely ill were investigated. Concentration detected by a fluorometric method di-amino-naphthalene using acidic hydrolysis. Selenoprotein P (Sepp1), malondialdehyde (MDA), 4-hydroxynonenal (4-HNE) their metabolite adducts evaluated spectrophotometric methods commercial assay kits. Results: Obtained results demonstrated that Sepp1 concentration acute significantly (p < 0.05 for p 0.001 Sepp1) decreased compared patients. However, patients, values close low limit norm European population. 4-HNE as marker lipid peroxidation increased group recovery 0.001). Conclusions: pathology is characterized induction suppression antioxidant defenses during phase. Lower levels higher reactive oxygen species reflect this imbalance, highlighting role disease's pathogenesis.

Язык: Английский

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Evaluation of oxidative stress level: reactive oxygen species, reduced glutathione, and D-dimer in patients hospitalized due to COVID-19

Redox Report, Год журнала: 2023, Номер 28(1), С. 1 - 6

Опубликована: Дек. 2, 2023

Elevated D-dimer levels at hospital admission may also indicate a higher likelihood of progressing to severe or critical state. This study aimed assess reactive oxygen species (ROS), non-enzymatic antioxidant reduced glutathione (GSH), and in COVID-19 patients upon admission, examining their association with mortality outcomes. Data was collected from the medical records 170 hospitalized referral unit between March 2020 December 2021. Patients were divided into two groups: ward bed group (n = 87), comprising 51% moderate clinical conditions, intensive care (ICU) 83), 49% conditions. The mean age 59.4 years, male predominance 52.4%. overall death rate 43%, 30.6% 69.4% group. average time symptom onset hospitalization 6.42 days. Results showed that non-survivors had high ROS counts, longer ICU stays, worse saturation admission. In conclusion, elevated contribute outcomes critically ill patients, potentially serving as specific sensitive predictors poor

Язык: Английский

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An Interplay between Oxidative Stress (Lactate Dehydrogenase) and Inflammation (Anisocytosis) Mediates COVID-19 Severity Defined by Routine Clinical Markers

Antioxidants, Год журнала: 2023, Номер 12(2), С. 234 - 234

Опубликована: Янв. 20, 2023

Viral infections activate the innate immune response and secretion of inflammatory cytokines. They also alter oxidative stress markers, which potentially can have an involvement in pathogenesis disease. The aim this research was to study role process assessed through lactate dehydrogenase (LDH) on severity COVID-19 measured by oxygen saturation (SaO2) putative interaction with inflammation. investigation enrolled 1808 patients (mean age 68 60% male) from HM Hospitals database. To explore interactions, a regression model mediation analyses were performed. lower SaO2 presented lymphopenia higher values neutrophils-to-lymphocytes ratio anisocytosis coefficient. showed between LDH anisocytosis, suggesting that high levels (>544 U/L) coefficient than 10% impact patients. Moreover, analysis revealed mediated 41% (p value = 0.001) effect cohort. This marker important infection should be considered for management treatment phenomena concerning within precision medicine strategy.

Язык: Английский

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Serum proteomics for the identification of biomarkers to flag predilection of COVID19 patients to various organ morbidities

Clinical Proteomics, Год журнала: 2024, Номер 21(1)

Опубликована: Ноя. 1, 2024

COVID19 is a pandemic that has affected millions around the world since March 2020. While many patients recovered completely with mild illness, succumbed to various organ morbidities. This heterogeneity in clinical presentation of infection posed challenge clinicians world. It therefore crucial identify specific organ-related morbidity for effective treatment and better patient outcomes. We have carried out serum-based proteomic experiments protein biomarkers can flag dysfunctions patients.

Язык: Английский

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