Mitochondrial respiratory analysis of cryopreserved PBMCs isolated from human blood

Cryobiology, Год журнала: 2025, Номер 118, С. 105212 - 105212

Опубликована: Фев. 13, 2025

Mitochondrial bioenergetics of PBMCs have been linked with several to the better understanding human diseases. Due complex logistics clinical studies, samples are often cryopreserved for later analysis. Current data is conflicting on whether cryopreservation negatively affects mitochondrial function PBMCs. We isolated and peripheral blood mononuclear cells (PBMCs) from whole tested using a substrate-uncoupler-inhibitor-titration protocol Oroboros instrument. After three months storage in medium (at -80°C), aspects were measured. demonstrate that did not adversely affect parameters (routine, leak, complex-I OXPHOS, cytochrome-c response, ETS capacity, contributions N S-pathways ETS, ROX, complex-IV activity coupling). Therefore, after at -80°C, PBMC-mitochondria fully coupled functional. Clinical studies may cryopreserve analyses.

Язык: Английский

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Deciphering the mechanisms, biochemistry, physiology, and social habits in the process of aging

Archives of Gerontology and Geriatrics Plus, Год журнала: 2023, Номер 1(1), С. 100003 - 100003

Опубликована: Дек. 25, 2023

Aging is a complex journey shaped by interactions among mechanisms, biochemistry, physiology, and social habits. This review explores aging's core facets uncovering the mechanisms driving cellular senescence, oxidative stress, telomere shortening, mitochondrial dysfunction. The also delves into biochemistry physiology of aging, shedding light on hormonal shifts, genetic influences, immune system modifications that contribute to aging process. It navigates terrain habits linked considering profound impact dietary choices, physical activity, engagement, stress management. These elements intricately intertwine, emphasizing holistic nature aging. interplay between underscores need for comprehensive understanding transcends single disciplines. Deciphering these factors significance personalized approaches healthy Tailoring interventions individual genetics, lifestyles, health needs emerges as critical strategy optimizing well-being in later life. In conclusion, this illuminates multifaceted urging embrace complexity interdependence. By fostering holistic, approach, individuals can navigate with grace, vitality, resilience.

Язык: Английский

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Sex differences in frailty among older adults

Experimental Gerontology, Год журнала: 2023, Номер 184, С. 112333 - 112333

Опубликована: Дек. 1, 2023

By definition, aging is a natural, gradual and continuous process. On the other hand, frailty reflects increase in vulnerability to stressors shortens time without disease (health span) while longevity refers length of life (lifespan). The average expectancy has significantly increased during last few decades. A longer lifespan been accompanied by an decreased independence older adults, with major differences existing between men women. For example, women tend live than but also experience higher rates disability. Sex prevent optimization lifestyle interventions therapies effectively frailty. are rooted complex interplay uncontrollable (genetic, epigenetic, physiological), controllable factors (psychosocial factors). Thus, understanding underlying causes sex essential for developing personalized promote healthy improve quality In this review, we have discussed key contributors knowledge gaps related

Язык: Английский

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Associations of the MIND Diet with Cardiometabolic Diseases and Their Risk Factors: A Systematic Review

Diabetes Metabolic Syndrome and Obesity, Год журнала: 2023, Номер Volume 16, С. 3353 - 3371

Опубликована: Окт. 1, 2023

Purpose: Recent studies have expanded the scope of research on Mediterranean-DASH Intervention for Neurodegenerative Delay (MIND) diet beyond its impact cognitive performance. These investigations specifically explored potential to provide protection against cardiometabolic diseases and associated risk factors, including obesity dyslipidemia. Methods: We systematically summarized evaluated all existing observational trial evidence MIND in relation their factors adults. PubMed, Embase, CINAHL Cochrane Library databases were searched extract original humans published until September 2023, without date restrictions. A total 491 initially retrieved, out which 23 met eligibility criteria included final review. Duplicated irrelevant screened by five independent reviewers using Rayyan platform. Quality assessment was ascertained Newcastle-Ottawa scale risk-of-bias tool (RoB 2) randomized trials. Results: Across different study designs, generally with an improvement anthropometric measures other outcomes, such as blood pressure, glycemic control, lipid profile, inflammation stroke. The effects eating pattern some cardiovascular are less conclusive. Conclusion: findings this systematic review support recommendation a strategy reduce Further well-designed long-term warranted. Keywords: diet, dietary patterns, diseases,

Язык: Английский

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Mitochondria: fundamental characteristics, challenges, and impact on aging

Biogerontology, Год журнала: 2024, Номер 25(6), С. 923 - 941

Опубликована: Авг. 28, 2024

Язык: Английский

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Molecular mechanism for combined toxicity of micro(nano)plastics and carbon nanofibers to freshwater microalgae Chlorella pyrenoidosa

Environmental Pollution, Год журнала: 2024, Номер 344, С. 123403 - 123403

Опубликована: Янв. 21, 2024

Язык: Английский

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Organ-Specific Mitochondrial Alterations Following Ischemia–Reperfusion Injury in Post-Cardiac Arrest Syndrome: A Comprehensive Review

Life, Год журнала: 2024, Номер 14(4), С. 477 - 477

Опубликована: Апрель 5, 2024

Background: Mitochondrial dysfunction, which is triggered by systemic ischemia–reperfusion (IR) injury and affects various organs, a key factor in the development of post-cardiac arrest syndrome (PCAS). Current research on PCAS primarily addresses generalized mitochondrial responses, resulting knowledge gap regarding organ-specific dynamics. This review focuses responses to IR injury, particularly examining brain, heart, kidneys, highlight potential therapeutic strategies targeting dysfunction enhance outcomes post-IR injury. Methods Results: We conducted narrative recent advancements related exhibit considerable variation across different organ systems, influenced unique structures, bioenergetics, antioxidative capacities. Each demonstrates distinct behaviors that have evolved fulfill specific metabolic functional needs. For example, cerebral mitochondria display dynamic can be both protective detrimental neuronal activity function during ischemic events. Cardiac show vulnerability IR-induced oxidative stress, while renal pattern fission fusion, closely linked their susceptibility acute kidney heterogeneity requires tailored interventions. Progress medicine, especially realms genomics metabolomics, paving way for innovative combat dysfunction. Emerging techniques such as transplantation hold revolutionize management resuscitation science. Conclusions: The investigation into pivotal realm research, within context PCAS. nuanced understanding holds promise revolutionizing management, addressing dysfunctions observed critical organs affected

Язык: Английский

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Targeting mitochondria for ovarian aging: new insights into mechanisms and therapeutic potential

Frontiers in Endocrinology, Год журнала: 2024, Номер 15

Опубликована: Июнь 17, 2024

Ovarian aging is a complex process characterized by decline in oocyte quantity and quality, directly impacting fertility overall well-being. Recent researches have identified mitochondria as pivotal players the of ovaries, influencing various hallmarks pathways governing this intricate process. In review, we discuss multifaceted role determining ovarian fate, outline mechanisms through which contribute to aging. Specifically, emphasize potential targeting mitochondrial dysfunction innovative therapeutic approaches, including antioxidants, metabolic improvement, biogenesis promotion, mitophagy enhancement, transfer, traditional Chinese medicine. These strategies hold promise effective means mitigate age-related preserve health. Drawing insights from advanced field, review provides deeper understanding interplay between function aging, offering valuable perspectives for development novel interventions aimed at preserving enhancing reproductive

Язык: Английский

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Mitochondrial Dysfunction and Sarcopenic Obesity: The Role of Exercise

Journal of Clinical Medicine, Год журнала: 2023, Номер 12(17), С. 5628 - 5628

Опубликована: Авг. 29, 2023

Sarcopenic obesity (SO) constitutes the coexistence of skeletal muscle mass loss (sarcopenia) and excess adiposity (obesity). It is mainly considered as a condition in elderly with health-threatening impacts ranging from frailty to mortality. Mitochondrial dysfunction consists one basic pathophysiological mechanisms leading development SO its consequences. Indirect indicators mitochondrial function, such VO2max exercise capacity, have been demonstrated be negatively affected individuals SO, while positive effect on function has widely proved; thus, this review, we aimed at investigating effects endurance, resistance, concurrent training indexes patients. The results clinical trials evaluated reveal chronic physical well biogenesis activity. concluded that utilizing systematic program includes both aerobic strength exercises can an effective strategy for managing promoting overall health these

Язык: Английский

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Elevated parkinsonism pathological markers in dopaminergic neurons with developmental exposure to atrazine

The Science of The Total Environment, Год журнала: 2023, Номер 908, С. 168307 - 168307

Опубликована: Ноя. 8, 2023

Язык: Английский

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