Dynamics of Free Radical Oxidative Processes During the Latent Period of Experimental Metastasizing to the Liver

Ural Medical Journal, Год журнала: 2024, Номер 23(5), С. 89 - 103

Опубликована: Окт. 18, 2024

Purpose — to investigate the dynamics of content antioxidant enzymes superoxide dismutase 1 (SOD1), glutathione peroxidase (GPO1), reductase (GR) and lipid peroxidation products diene conjugates (DC), malondialdehyde (MDA) in spleen liver during latent period growth metastasis experimental tumor. Materials methods . Using 28 white male rats, a model hematogenous was created by transplanting sarcoma 45 cells (S45) into spleen, previously lead out under skin 3 weeks before. Previously, determined that tumor visualized at 5 weeks, metastases 7 after transplantation S45. Levels SOD1, GPO1, GR MDA were using ELISA DC biochemical method homogenates (1–2 post-transplantation). Results Significant changes (1.5–5.2 times, р < 0.050–0.001) studied factors levels observed compared intact rats with out. Activation system noted (tumor-carrying organ) metastasis. At same time, (the target organ metastasis) also increased but simultaneously pronounced decreased (5 p 0.001) without affecting SOD1 levels. Conclusion Liver tissue exhibited inferiority protection formation pro-oxidant condition growth, which may prepare soil for

Язык: Английский

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Bioinspired Iron Porphyrin Covalent Organic Frameworks-Based Nanozymes Sensor Array: Machine Learning-Assisted Identification and Detection of Thiols

ACS Applied Materials & Interfaces, Год журнала: 2024, Номер 16(51), С. 71048 - 71059

Опубликована: Дек. 12, 2024

Given the crucial role of thiols in maintaining normal physiological functions, it is essential to establish a high-throughput and sensitive analytical method identify quantify various accurately. Inspired by iron porphyrin active center natural horseradish peroxidase (HRP), we designed synthesized two covalent organic frameworks (Fe-COF-H Fe-COF-OH) with notable peroxidase-like (POD) activity, capable catalyzing 3,3′,5,5′-tetramethylbenzidine (TMB) into oxidized TMB three distinct absorption peaks. Based on these, six-channel nanozyme colorimetric sensor array was constructed, which could map specific fingerprints thiols. Subsequently, machine learning techniques, including supervised linear discriminant analysis (LDA), decision trees (DT) artificial neural networks (ANN), unsupervised hierarchical cluster (HCA), ensemble random forests (RF), were used for precise identification complex systems, detection limit as low 50 nM. Significantly, demonstrated strong potential practical applications, analyzing homocysteine (Hcy) human serum, mercaptoacetic acid (TGA) depilatory creams, glutathione (GSH) cell lysates, thereby showing promise use disease diagnosis.

Язык: Английский

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African swine fever virus enhances viral replication by increasing intracellular reduced glutathione levels, which suppresses stress granule formation

Veterinary Research, Год журнала: 2024, Номер 55(1)

Опубликована: Дек. 20, 2024

Abstract African swine fever virus (ASFV) is a DNA that has significantly impacted the global industry. Currently, there are no effective therapies or vaccines against ASFV. Stress granules (SGs), known for their antiviral properties, not induced during ASFV infection, even though reactive oxygen species (ROS) generated. The mechanism by which regulates SGs formation remains unclear. This study demonstrates antagonises and increases intracellular levels of reduced glutathione (GSH) levels. use GSH inhibitor BSO activator NAC confirmed ASFV-induced increase in helps to suppress influences viral replication. Additionally, this revealed enhances upregulating antioxidant transcription factor NRF2, as well factors involved synthesis regeneration, such GCLC, those related ferroptosis pathway, SLC7A11. Furthermore, uncovered manipulates activating mitochondrial protein AIFM1. regulatory inhibit SGs, thereby creating an optimal environment These findings provide new insights into molecular strategies employed

Язык: Английский

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The Effects of Different Respiratory Viruses on the Oxidative Stress Marker Levels in an In Vitro Model: A Pilot Study

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(22), С. 12088 - 12088

Опубликована: Ноя. 11, 2024

Respiratory viruses are among the most common causes of human infections. Examining pathological processes linked to respiratory viral infections is essential for diagnosis, treatment strategies, and developing novel therapeutics. Alterations in oxidative stress levels homeostasis significant associated with The study aimed compare selected markers: total status (TOS), antioxidant capacity (TAC), index (OSI) glutathione peroxidase (GPx) reductase (GR) activities normal (MRC5 cell line) tumor (A549 lung cells infected coronaviruses (HCoV) OC43 229E, adenovirus type 5 (HAdV5), or rhinovirus A (HRV A). We observed that a infection more significantly affected non-enzymatic markers adenocarcinoma model cells), while fibroblasts (MRC-5 presented changes enzymatic markers. suggest further detailed research required analyze this phenomenon.

Язык: Английский

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Boldine reduces left ventricle oxidative stress in isoproterenol-induced adrenergic overload experimental model

Archives of Physiology and Biochemistry, Год журнала: 2024, Номер unknown, С. 1 - 10

Опубликована: Дек. 22, 2024

Sustained adrenergic overload in the heart causes maladaptive cardiac remodelling, which involves oxidative stress. Boldine (BOL) has antioxidant activity and represents a novel therapeutic approach. This study explored cardioprotective role of BOL adverse left ventricular remodelling induced by isoproterenol. The rats were divided into four groups: control; (25 mg/kg daily); isoproterenol (ISO) (5 daily), ISO + BOL. Morphometric, echocardiographic, stress parameters evaluated. attenuated both hypertrophy increased diastolic volume caused overstimulation (P < 0.05). treatment reduced lipid peroxidation (ISO vs. BOL; P 0.05), this effect was associated with superoxide dismutase (SOD) 0.05) glutathione-S-transferase levels (GST) data suggest that may improve attenuate some remodelling.

Язык: Английский

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Network-based multi-omics-disease-drug associations reveal drug repurposing candidates for COVID-19 disease phases

Опубликована: Апрель 16, 2024

Background: The development and roll-out of vaccines, the use various drugs have contributed to controlling COVID-19 pandemic. Nevertheless, challenges such as inequitable distribution influence emerging viral lineages immune evasive variants on vaccine efficacy, inadequate defense in subgroups population continue motivate new combat disease. Aim: In this study, we sought identify, prioritize, characterize drug repurposing candidates appropriate for treating mild, moderate, or severe using a network-based integrative approach that systematically integrates drug-related data multi-omics datasets. Methods : We leveraged data, used random walk restart algorithm explore an integrated knowledge graph comprised three sub-graphs: (i) graph, (ii) (iii) disease-state specific omics graph. Results: prioritized twenty FDA-approved agents potential candidate disease phases. Specifically, could stimulate cell recruitment activation including histamine, curcumin, paclitaxel utility mild states mitigate progression. Drugs like omacetaxine, crizotinib, vorinostat exhibit antiviral properties inhibit replication can be considered moderate states. Also, given association between antioxidant deficiency high inflammatory factors trigger cytokine storms, antioxidants glutathione potent anti-inflammatory effects (sarilumab tocilizumab), corticosteroids (dexamethasone hydrocortisone), immunosuppressives (sirolimus cyclosporine) are hyperinflammatory cascade COVID-19. Conclusion: Our study demonstrates data-driven analysis within enables prioritizing phases, offering comprehensive basis therapeutic strategies brought market quickly their established safety profiles. Importantly, implemented here prioritize other diseases.

Язык: Английский

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Effects of N-Acetylcysteine Supplementation on Semen Analysis, Hormonal Profile and Spontaneous Pregnancy Rate in Idiopathic Infertile Men: Before and After Clinical Trial

Deleted Journal, Год журнала: 2024, Номер 76(3), С. 125 - 134

Опубликована: Март 1, 2024

Objective: To compare sperm quality and quantity, hormonal profiles spontaneous pregnancy rates before after administering a 3 months course of N-Acetylcysteine (NAC) Material Methods: This prospective clinical trial was conducted at the Infertility Unit Obstetrics Gynecology Department Bhumibol Adulyadej Hospital, Thailand. The study period from June 1, 2023 to September 30, 2023. Subjects were idiopathic infertile males aged between 20 50 years old. All subjects received 600 mg NAC orally per day. Semen analysis (SA) male (MHP; testosterone, LH, FSH prolactin) performed three administration. Demographic, characters laboratory change recorded. Results: 92 participants recruited. mean age couples 34.5 average duration infertility 3.6 years. Increase semen volume (2.1 vs 2.4 ml, p < 0.001), concentration (30.5 43.1 x 106/mL, total motility (59.57 72.38 %, progressive (59 69.8 normozoospermia (60 83 testosterone (452.8 479.0 ng/dL, = 0.038), LH (4.6 5.3 mIU/mL, 0.004) (4.4 4.6 0.009) observed No changes in morphology prolactin level. One-third (27/92) each participant’s spouse conceived spontaneously. Conclusion: potentially enhances profiles, quantity with an impressive rate.

Язык: Английский

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Viral Infections and the Glutathione Peroxidase Family: Mechanisms of Disease Development

Antioxidants and Redox Signaling, Год журнала: 2024, Номер unknown

Опубликована: Окт. 24, 2024

The glutathione peroxidase (GPx) family is recognized for its essential function in maintaining cellular redox balance and countering the overproduction of reactive oxygen species (ROS), a process intricately linked to progression various diseases including those spurred by viral infections. modulation GPx activity viruses presents critical juncture disease pathogenesis, influencing responses trajectory infection-induced diseases.

Язык: Английский

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Andrographolide attenuates SARS-CoV-2 infection via an up-regulation of glutamate-cysteine ligase catalytic subunit (GCLC)

Phytomedicine, Год журнала: 2024, Номер 136, С. 156279 - 156279

Опубликована: Ноя. 30, 2024

Язык: Английский

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