Co-delivery of antioxidants and siRNA-VEGF: promising treatment for age-related macular degeneration
Drug Delivery and Translational Research,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 3, 2025
Язык: Английский
Association between four anthropometric indices with age-related Macular Degeneration from NHANES 2005–2008
Lipids in Health and Disease,
Год журнала:
2025,
Номер
24(1)
Опубликована: Янв. 11, 2025
Age-related
macular
degeneration
(AMD)
decrease
vision
and
presents
considerable
challenges
for
both
public
health
clinical
management
strategies.
Obesity
is
usually
implicated
with
increased
AMD,
body
mass
index
(BMI)
does
not
reflect
fat
distribution.
An
array
of
studies
has
indicated
a
robust
relationship
between
distribution
obesity.
This
research
to
evaluate
the
anthropometric
measurements
AMD
in
United
States
citizens
cohort
patients.
Our
study
included
3,127
participants,
all
whom
were
selected
from
National
Health
Nutrition
Examination
Survey
(NHANES)
conducted
2005
2008.
Various
indices,
including
weight
(WT),
waist
circumference
(WC),
Body
Mass
Index
(BMI),
waist-to-height
ratio
(WtHR),
circularity
(CI),
weight-adjusted
(WWI),
roundness
(BRI),
size
(ABSI),
visceral
adiposity
(VAI),
have
been
studied
extensively
within
nutrition
assess
Odds
ratios
(OR)
each
index,
relation
its
different
stages,
computed,
adjusting
confounding
variables.
Smoothed
curve
fitting,
coupled
weighted
multivariable
logistic
regression
analysis,
was
used
examine
impact
these
measures
on
prevalence
AMD.
Subgroup
analyses
according
gender,
age,
BMI,
drinking,
smoking,
CVD,
diabetes,
hypertension,
cataract
operation,
glaucoma.
After
variables,
significant
positive
correlations
observed
WtHR
(OR
=
1.237
(1.065-1.438)),
BRI
1.221
(1.058-1.410)),
CI
1.189
(1.039-1.362)),
WWI
1.250
(1.095-1.425))
particularly
early
However,
no
effects
indicators
late
exhibited
linear
Two-segment
modeling
revealed
nonlinear
associations
WtHR,
BRI,
The
association
more
pronounced
excessive
alcohol
consumption
CI,
(P
interaction
0.0033,
0.0021,
0.0194,
0.0022,
respectively).
Additionally,
stronger
females
0.0146
0.0117,
Furthermore,
associated
non-smokers
0.0402).
confirmed
risk
four
measures,
WWI,
especially
findings
suggest
that
indices
should
be
broadly
utilized
improve
prevention
treatment
we
found
measurement
individuals
high
consumption.
Язык: Английский
Melatonin protects retinal pigment epithelium cells against ferroptosis in AMD via the PI3K/AKT/MDM2/P53 pathway
Frontiers in Pharmacology,
Год журнала:
2025,
Номер
16
Опубликована: Фев. 27, 2025
Oxidative
stress-prompted
degeneration
of
the
retinal
pigment
epithelium
(RPE)
notably
contributes
to
onset
age-related
macular
(AMD).
However,
pathways
leading
RPE
deterioration
and
possible
preventative
strategies
are
not
yet
completely
comprehended.
Ferroptosis
was
assayed
through
evaluation
lipid
peroxidation
(C11-BODIPY
MDA),
reactive
oxygen
species
(ROS),
transmission
electron
microscopy
(TEM),
iron
content
measurement,
q-PCR,
western
blotting,
immunofluorescence.
To
assess
structure
function
in
mice,
ERG
(electroretinography),
OCT
(optical
coherence
tomography),
H&E
(hematoxylin
eosin)
staining
were
employed.
Network
pharmacology
methods
utilized
elucidate
potential
mechanisms
underlying
melatonin's
protective
effects
against
ferroptosis
cells
AMD.
Genetic
engineering
techniques
applied
investigate
regulatory
relationships
among
phosphatidylinositol
3-kinase
(PI3K),
protein
kinase-B
(AKT),
murine
double
minute-2
(MDM2),
53
(P53),
solute
carrier
family
7
member
11
(SLC7A11).
In
vitro
knockdown
experiments
MDM2
conducted
explore
its
role
within
cells.
Aβ1-40
can
trigger
Melatonin
inhibit
oxidative
stress
induced
by
exhibits
a
effect
on
Aβ1-40-induced
AMD,
significantly
improving
mouse
retina
layer,
facilitating
restoration
visual
function.
revealed
that
targets
melatonin
AMD
closely
related
ferroptosis,
indicated
predominant
associated
with
PI3K/AKT/MDM2/P53
signaling
pathway.
Knocking
down
specific
expression
weaken
inhibitory
ferroptosis.
suppress
cell
death
via
pathway,
thereby
preventing
decelerating
progression
Язык: Английский
Phlorizin Protects Against Oxidative Stress and Inflammation in Age-Related Macular Degeneration Model
Biomolecules,
Год журнала:
2025,
Номер
15(4), С. 523 - 523
Опубликована: Апрель 3, 2025
Background:Sweet
Tea
(Lithocarpus
polystachyus
Rehd.),
a
traditional
ethnobotanical
medicine,
contains
phlorizin,
dihydrochalcone
compound
with
antioxidative
and
anti-inflammatory
properties.
Given
the
critical
role
of
oxidative
stress
inflammation
in
age-related
macular
degeneration
(AMD),
this
study
tested
hypothesis
that
phlorizin
mitigates
damage
AMD
models,
thereby
offering
therapeutic
potential.
Materials
Methods:
Adult
retinal
pigmented
epithelial
cells
(ARPE-19)
were
pre-treated
(0.01–0.1
μM)
subjected
to
induced
by
ultraviolet
A
(UVA)
radiation
or
sodium
iodate
(NaIO3).
Cell
viability,
reactive
oxygen
species
(ROS)
production,
MAPK/NF-κB
signaling,
level
pro-inflammatory
cytokines
(IL-1β,
IL-6,
TNF-α)
pro-angiogenic
factors
(VEGF,
MMP2,
MMP9)
expression
assessed
using
MTT
assays,
fluorescence
imaging,
Western
blotting,
RT-qPCR.
In
vivo,
laser-induced
choroidal
neovascularization
(CNV)
mouse
model
was
used
evaluate
phlorizin’s
effects
on
CNV
formation
vascular
leakage
via
fundus
photography
angiography.
Result:
Phlorizin
significantly
enhanced
cell
reduced
ROS
inhibited
activation,
downregulated
inflammatory
angiogenic
mediators.
vivo
studies
confirmed
following
treatment.
Conclusions:
demonstrated
significant
protective
against
inflammation,
highlighting
its
potential
for
treating
AMD.
Язык: Английский
The role of peripheral blood microRNAs in the pathogenesis and treatment response of age-related macular degeneration
Future Science OA,
Год журнала:
2025,
Номер
11(1)
Опубликована: Апрель 4, 2025
Age-related
macular
degeneration
is
a
leading
cause
of
vision
loss
in
aging
populations,
driven
by
complex
interactions
between
genetic,
environmental,
and
molecular
factors.
MicroRNAs
have
emerged
as
crucial
regulators
cellular
processes
such
oxidative
stress,
inflammation,
angiogenesis,
all
which
contribute
to
AMD
pathogenesis.
This
narrative
review
aims
summarize
the
involvement
peripheral
blood
microRNAs
pathogenesis
AMD,
focusing
on
key
pathways
angiogenesis.
Additionally,
it
explores
their
potential
biomarkers
for
predicting
treatment
response,
particularly
anti-VEGF
therapies.
The
miRNAs
noninvasive
early
diagnosis
personalized
strategies
also
explored,
highlighting
future
directions
research.
Язык: Английский
Ocular immune‐related diseases: molecular mechanisms and therapy
MedComm,
Год журнала:
2024,
Номер
5(12)
Опубликована: Ноя. 28, 2024
Abstract
Ocular
immune‐related
diseases,
represent
a
spectrum
of
conditions
driven
by
immune
system
dysregulation,
include
but
not
limit
to
uveitis,
diabetic
retinopathy,
age‐related
macular
degeneration,
Graves’
ophthalmopathy,
etc.
The
molecular
and
cellular
mechanisms
underlying
these
diseases
are
typically
dysfunctioned
responses
targeting
ocular
tissues,
resulting
in
inflammation
tissue
damage.
Recent
advances
have
further
elucidated
the
pivotal
role
different
development,
progression,
as
well
management
various
diseases.
However,
there
is
currently
relative
lack
connection
between
treatments
several
In
this
review,
we
discuss
recent
findings
related
immunopathogenesis
above‐mentioned
particular,
summarize
types
cells,
inflammatory
mediators,
associated
signaling
pathways
that
involved
pathophysiology
ophthalmopathies.
Furthermore,
also
future
directions
utilizing
anti‐inflammatory
regime
This
will
facilitate
better
understanding
pathogenesis
provide
new
insights
for
treatment
approaches.
Язык: Английский
Potential Use of Plasma Rich in Growth Factors in Age-Related Macular Degeneration: Evidence from a Mouse Model
Medicina,
Год журнала:
2024,
Номер
60(12), С. 2036 - 2036
Опубликована: Дек. 10, 2024
Background
and
Objectives:
Age-related
macular
degeneration
(AMD)
is
the
leading
cause
of
low
vision
legal
blindness
in
adults
developed
countries.
Wet
AMD
can
be
successfully
treated
using
vascular
endothelial
growth
factor
(VEGF)
inhibitors;
however,
dry
currently
has
no
effective
treatment.
The
purpose
this
study
to
analyze
efficacy
intraocular
injection
plasma
rich
factors
(PRGF)
an
mouse
model
induced
by
intraperitoneal
administration
sodium
iodate.
Materials
Methods:
Intravitreal
application
PRGF
(experimental
group)
saline
(control
was
performed
immediately
after
Retinographies
were
at
2
7
days
treatment
administration.
eyes
retrieved
for
histological
immunohistological
analysis.
Statistical
analysis
compare
outcomes
between
groups.
Results:
In
comparison
solution,
significantly
decreased
depigmentation
RPE,
showing
a
more
reddened
retina.
intravitreal
reduced
glial
fibrillary
acidic
protein
(GFAP)
stained
processes,
suggesting
significant
reduction
risk
scar
formation.
Moreover,
myofibroblast
invasion
into
RPE
cell
layer
PRGF-treated
group
mice.
There
tendency
better
preservation
photoreceptors
group.
Conclusions:
Within
limitations
study,
provided
protection
against
systemic
NaIO3.
Язык: Английский