Co-delivery of antioxidants and siRNA-VEGF: promising treatment for age-related macular degeneration

Drug Delivery and Translational Research, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 3, 2025

Language: Английский

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Association between four anthropometric indices with age-related Macular Degeneration from NHANES 2005–2008

Lipids in Health and Disease, Journal Year: 2025, Volume and Issue: 24(1)

Published: Jan. 11, 2025

Age-related macular degeneration (AMD) decrease vision and presents considerable challenges for both public health clinical management strategies. Obesity is usually implicated with increased AMD, body mass index (BMI) does not reflect fat distribution. An array of studies has indicated a robust relationship between distribution obesity. This research to evaluate the anthropometric measurements AMD in United States citizens cohort patients. Our study included 3,127 participants, all whom were selected from National Health Nutrition Examination Survey (NHANES) conducted 2005 2008. Various indices, including weight (WT), waist circumference (WC), Body Mass Index (BMI), waist-to-height ratio (WtHR), circularity (CI), weight-adjusted (WWI), roundness (BRI), size (ABSI), visceral adiposity (VAI), have been studied extensively within nutrition assess Odds ratios (OR) each index, relation its different stages, computed, adjusting confounding variables. Smoothed curve fitting, coupled weighted multivariable logistic regression analysis, was used examine impact these measures on prevalence AMD. Subgroup analyses according gender, age, BMI, drinking, smoking, CVD, diabetes, hypertension, cataract operation, glaucoma. After variables, significant positive correlations observed WtHR (OR = 1.237 (1.065-1.438)), BRI 1.221 (1.058-1.410)), CI 1.189 (1.039-1.362)), WWI 1.250 (1.095-1.425)) particularly early However, no effects indicators late exhibited linear Two-segment modeling revealed nonlinear associations WtHR, BRI, The association more pronounced excessive alcohol consumption CI, (P interaction 0.0033, 0.0021, 0.0194, 0.0022, respectively). Additionally, stronger females 0.0146 0.0117, Furthermore, associated non-smokers 0.0402). confirmed risk four measures, WWI, especially findings suggest that indices should be broadly utilized improve prevention treatment we found measurement individuals high consumption.

Language: Английский

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Melatonin protects retinal pigment epithelium cells against ferroptosis in AMD via the PI3K/AKT/MDM2/P53 pathway

Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 27, 2025

Oxidative stress-prompted degeneration of the retinal pigment epithelium (RPE) notably contributes to onset age-related macular (AMD). However, pathways leading RPE deterioration and possible preventative strategies are not yet completely comprehended. Ferroptosis was assayed through evaluation lipid peroxidation (C11-BODIPY MDA), reactive oxygen species (ROS), transmission electron microscopy (TEM), iron content measurement, q-PCR, western blotting, immunofluorescence. To assess structure function in mice, ERG (electroretinography), OCT (optical coherence tomography), H&E (hematoxylin eosin) staining were employed. Network pharmacology methods utilized elucidate potential mechanisms underlying melatonin's protective effects against ferroptosis cells AMD. Genetic engineering techniques applied investigate regulatory relationships among phosphatidylinositol 3-kinase (PI3K), protein kinase-B (AKT), murine double minute-2 (MDM2), 53 (P53), solute carrier family 7 member 11 (SLC7A11). In vitro knockdown experiments MDM2 conducted explore its role within cells. Aβ1-40 can trigger Melatonin inhibit oxidative stress induced by exhibits a effect on Aβ1-40-induced AMD, significantly improving mouse retina layer, facilitating restoration visual function. revealed that targets melatonin AMD closely related ferroptosis, indicated predominant associated with PI3K/AKT/MDM2/P53 signaling pathway. Knocking down specific expression weaken inhibitory ferroptosis. suppress cell death via pathway, thereby preventing decelerating progression

Language: Английский

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Phlorizin Protects Against Oxidative Stress and Inflammation in Age-Related Macular Degeneration Model

Biomolecules, Journal Year: 2025, Volume and Issue: 15(4), P. 523 - 523

Published: April 3, 2025

Sweet Tea (Lithocarpus polystachyus Rehd.), a traditional ethnobotanical medicine, contains phlorizin, dihydrochalcone compound with antioxidative and anti-inflammatory properties. Given the critical role of oxidative stress inflammation in age-related macular degeneration (AMD), this study tested hypothesis that phlorizin mitigates damage AMD models, thereby offering therapeutic potential. Adult retinal pigmented epithelial cells (ARPE-19) were pre-treated (0.01-0.1 μM) subjected to induced by ultraviolet A (UVA) radiation or sodium iodate (NaIO3). Cell viability, reactive oxygen species (ROS) production, MAPK/NF-κB signaling, level pro-inflammatory cytokines (IL-1β, IL-6, TNF-α) pro-angiogenic factors (VEGF, MMP2, MMP9) expression assessed using MTT assays, fluorescence imaging, Western blotting, RT-qPCR. In vivo, laser-induced choroidal neovascularization (CNV) mouse model was used evaluate phlorizin's effects on CNV formation vascular leakage via fundus photography angiography. Phlorizin significantly enhanced cell reduced ROS inhibited activation, downregulated inflammatory angiogenic mediators. vivo studies confirmed following treatment. demonstrated significant protective against inflammation, highlighting its potential for treating AMD.

Language: Английский

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The role of peripheral blood microRNAs in the pathogenesis and treatment response of age-related macular degeneration

Future Science OA, Journal Year: 2025, Volume and Issue: 11(1)

Published: April 4, 2025

Age-related macular degeneration is a leading cause of vision loss in aging populations, driven by complex interactions between genetic, environmental, and molecular factors. MicroRNAs have emerged as crucial regulators cellular processes such oxidative stress, inflammation, angiogenesis, all which contribute to AMD pathogenesis. This narrative review aims summarize the involvement peripheral blood microRNAs pathogenesis AMD, focusing on key pathways angiogenesis. Additionally, it explores their potential biomarkers for predicting treatment response, particularly anti-VEGF therapies. The miRNAs noninvasive early diagnosis personalized strategies also explored, highlighting future directions research.

Language: Английский

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L-shaped association between oxidative balance score and vision-related functional burden in adults in the United States, NHANES 2005–2008

Frontiers in Nutrition, Journal Year: 2025, Volume and Issue: 12

Published: May 2, 2025

Objective This study aims to investigate the relationship between oxidative balance score (OBS) and vision-related functional burden (VRFB) in US adults. Methods The utilized data from two consecutive cycles of National Health Nutrition Examination Survey (NHANES) 2005 2008. A multivariate weighted logistic regression model was employed explore OBS VRFB general population subgroups, while interaction effects were tested with a likelihood test. Restricted cubic spline assess nonlinear association VRFB. Sensitivity analyses performed test robustness results. Results total 6,682 participants aged 20 years older included NHANES database. negative observed fully adjusted model, an odds ratio (OR) 0.968 [95% confidence interval (CI): 0.949–0.987]. Compared lowest quartile OBS, second (OR: 0.669, 95%CI: 0.486–0.922), third 0.589, 0.403–0.859), highest 0.554, 0.359–0.855) quartiles associated reduced risk An L-shaped displayed ( P for = 0.016). Notably, when analysis stratified by employment status, significant < 0.05). protective effect more pronounced among unemployed. results found be robust sensitivity analyses. Conclusion U.S. adults older. novel finding suggests that maintaining favorable through modifiable dietary lifestyle (such as increased physical activity, smoking cessation, alcohol consumption) may help protect vision. We also stronger provides new insights into prevention vision loss, highlighting importance not only factors but considering different such Further longitudinal studies are required further validate these observations elucidate underlying mechanisms, particularly elucidating causal relationships variables, controlling confounding factors, examining development visual function.

Language: Английский

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Ocular immune‐related diseases: molecular mechanisms and therapy

MedComm, Journal Year: 2024, Volume and Issue: 5(12)

Published: Nov. 28, 2024

Abstract Ocular immune‐related diseases, represent a spectrum of conditions driven by immune system dysregulation, include but not limit to uveitis, diabetic retinopathy, age‐related macular degeneration, Graves’ ophthalmopathy, etc. The molecular and cellular mechanisms underlying these diseases are typically dysfunctioned responses targeting ocular tissues, resulting in inflammation tissue damage. Recent advances have further elucidated the pivotal role different development, progression, as well management various diseases. However, there is currently relative lack connection between treatments several In this review, we discuss recent findings related immunopathogenesis above‐mentioned particular, summarize types cells, inflammatory mediators, associated signaling pathways that involved pathophysiology ophthalmopathies. Furthermore, also future directions utilizing anti‐inflammatory regime This will facilitate better understanding pathogenesis provide new insights for treatment approaches.

Language: Английский

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Potential Use of Plasma Rich in Growth Factors in Age-Related Macular Degeneration: Evidence from a Mouse Model

Medicina, Journal Year: 2024, Volume and Issue: 60(12), P. 2036 - 2036

Published: Dec. 10, 2024

Background and Objectives: Age-related macular degeneration (AMD) is the leading cause of low vision legal blindness in adults developed countries. Wet AMD can be successfully treated using vascular endothelial growth factor (VEGF) inhibitors; however, dry currently has no effective treatment. The purpose this study to analyze efficacy intraocular injection plasma rich factors (PRGF) an mouse model induced by intraperitoneal administration sodium iodate. Materials Methods: Intravitreal application PRGF (experimental group) saline (control was performed immediately after Retinographies were at 2 7 days treatment administration. eyes retrieved for histological immunohistological analysis. Statistical analysis compare outcomes between groups. Results: In comparison solution, significantly decreased depigmentation RPE, showing a more reddened retina. intravitreal reduced glial fibrillary acidic protein (GFAP) stained processes, suggesting significant reduction risk scar formation. Moreover, myofibroblast invasion into RPE cell layer PRGF-treated group mice. There tendency better preservation photoreceptors group. Conclusions: Within limitations study, provided protection against systemic NaIO3.

Language: Английский

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