Total Oxidant and Antioxidant Capacity of Gingival Crevicular Fluid and Saliva in Patients with Periodontitis: Review and Clinical Study

Antioxidants, Год журнала: 2020, Номер 9(5), С. 450 - 450

Опубликована: Май 23, 2020

Periodontitis is inextricably linked to oxidative-reductive (redox) imbalance. However, little still known about the resultant ability scavenge oxygen free radicals in saliva and gingival crevicular fluid patients with periodontitis. The multitude of enzymatic non-enzymatic antioxidants their synergistic effects cause an interest evaluation total antioxidative capacity. Thus, our study aimed evaluate oxidative activity periodontitis, as well relate these biomarkers clinical indices periodontopathy. Additionally, by calculating stress index (OSI), intensity redox disturbances was also evaluated. Fifty-eight periodontitis were included divided into two subgroups depending on severity disease. In non-stimulated/stimulated a group, we found significantly higher OSI oxidant status (TOS) lower antioxidant capacity (TAC). reduce iron ions (FRAP) only stimulated non-stimulated examined parameters correlated periodontium’s condition, which indicates exacerbation inflammatory process. TAC, TOS, OSI, FRAP did not differentiate individual stages

Язык: Английский

---

Salivary Outer Membrane Vesicles and DNA Methylation of Small Extracellular Vesicles as Biomarkers for Periodontal Status: A Pilot Study

International Journal of Molecular Sciences, Год журнала: 2021, Номер 22(5), С. 2423 - 2423

Опубликована: Фев. 28, 2021

Periodontitis is an inflammatory disease, associated with a microbial dysbiosis. Early detection using salivary small extracellular vesicles (sEVs) biomarkers may facilitate timely prevention. sEVs derived from different species (i.e., humans, bacteria) are expected to circulate in saliva. This pilot study recruited 22 participants (seven periodontal healthy, seven gingivitis and eight periodontitis) were isolated the size-exclusion chromatography (SEC) method. The periodontitis groups compared terms of CD9+ sEV subpopulation, Gram-negative bacteria-enriched lipopolysaccharide (LPS+) outer membrane (OMVs) global DNA methylation pattern 5-methylcytosine (5mC), 5-hydroxymethylcytosine (5hmC) N6-Methyladenosine (m6dA). It was found that LPS+ OMVs, 5mC four pathogens (T. denticola, E. corrodens, P. gingivalis F. nucleatum) secreted OMVs significantly increased those healthy groups. These differences more pronounced than whole saliva superior distinguishing gingivitis, comparison patients. Of note, hypermethylation can distinguish patients both controls high sensitivity specificity (AUC = 1). research findings suggest assessing be useful biomarker for periodontitis.

Язык: Английский

---

Pathways Linking Oral Bacteria, Nitric Oxide Metabolism, and Health

Journal of Dental Research, Год журнала: 2022, Номер 101(6), С. 623 - 631

Опубликована: Янв. 26, 2022

Nitrate-reducing oral bacteria have gained a lot of interest due to their involvement in nitric oxide (NO) synthesis and its important cardiometabolic outcomes. Consortia nitrate-metabolizing associated with health cognitive function been recently identified. Longitudinal studies clinical trials shown that chronic mouthwash use is increased blood pressure risk for prediabetes/diabetes hypertension. Concurrently, recent are beginning shed some light on the complexity nitrate reduction pathways bacteria, such as dissimilatory ammonium (DNRA), which converts nitrite into ammonium, denitrification, NO, nitrous oxide, dinitrogen. These can affect composition metabolism microbiome; consequently, salivary proposed targets probiotics health. could also systemic NO levels because generated through denitrification be oxidized back saliva, thus facilitating flux along 3 – -NO 2 pathway, while DNRA leading reduced NO. It is, therefore, understand pathway predominates under different environmental conditions, since consequences Recent show hygiene measures tongue cleaning dietary likely favor denitrifying Neisseria, linked better A vast body literature demonstrates redox potential, carbon-to-nitrate ratio, nitrate-to-nitrite ratio key drivers competing various natural artificial ecosystems. Based this information, novel behavioral microbial model proposed, links lifestyle factors metabolism.

Язык: Английский

---

Salivary Biomarkers of Oxidative Stress and Inflammation in Stroke Patients: From Basic Research to Clinical Practice

Oxidative Medicine and Cellular Longevity, Год журнала: 2021, Номер 2021(1)

Опубликована: Янв. 1, 2021

Cerebral stroke is a serious worldwide health problem, as can be seen by the global epidemic of disease. In this disorder, when blood flow compromised ruptures or blocked arteries, sudden death neurons observed result lack oxygen and nutrients. Numerous severe problems frequent complications also exist in patients; therefore, there an urgent need to develop new therapeutic, diagnostic, prognostic methods for At present, diagnosis based on neurological examination, medical history, neuroimaging, due fact that rapid noninvasive diagnostic tests are unavailable. Nevertheless, oxidative stress inflammation considered key factors pathogenesis. Oxygen free radicals responsible oxidation lipids, proteins, DNA/RNA, which turn contributes damage brain. Toxic products reactions act cytostatically cell damaging membranes leading neuronal apoptosis necrosis. Thus, it seems redox/inflammatory biomarkers might used Nowadays, saliva increasing interest clinical laboratory medicine. Redox could obtained easily, noninvasively, cheaply, stress‐free from saliva. This minireview aimed at presenting current knowledge concerning use salivary prognosis stroke.

Язык: Английский

---

Comprehensive Evaluation of the Oral Health Status, Salivary Gland Function, and Oxidative Stress in the Saliva of Patients with Subacute Phase of Stroke: A Case-Control Study

Journal of Clinical Medicine, Год журнала: 2020, Номер 9(7), С. 2252 - 2252

Опубликована: Июль 15, 2020

This is the first study to assess, comprehensively, oral health status; salivary glands’ function and enzymatic non-enzymatic antioxidant defense; oxidative damage proteins lipids in non-stimulated (NWS) stimulated (SWS) whole saliva of stroke patients. The included 30 patients subacute phase an age gender-matched control group. We showed that activity enzymes (catalase peroxidase) was significantly higher both NWS SWS patients, similarly uric acid concentration. However, group, reduced glutathione (GSH) concentration decreased. contents protein glycooxidation products (advanced glycation end (AGE) oxidation (AOPP)) lipid hydroperoxides were In group there also a decrease secretion total content. Interestingly, correlate negatively with flow. ROC analysis GSH 100% specificity sensitivity differentiates analyzed groups (AUC = 1.0). To sum up, are redox imbalances saliva. Stroke suffer from gland dysfunction.

Язык: Английский

---

The Effect of Selected Dental Materials Used in Conservative Dentistry, Endodontics, Surgery, and Orthodontics as Well as during the Periodontal Treatment on the Redox Balance in the Oral Cavity

International Journal of Molecular Sciences, Год журнала: 2020, Номер 21(24), С. 9684 - 9684

Опубликована: Дек. 18, 2020

Oxidative stress (OS) is a redox homeostasis disorder that results in oxidation of cell components and thus disturbs metabolism. OS induced by numerous internal as well external factors. According to recent studies, dental treatment may also be one them. The aim our work was assess the effect on balance oral cavity. We reviewed literature available PubMed, Medline, Scopus databases, including from 2010 2020. Publications were searched according keywords: oxidative monomers; amalgam; periodontitis, braces, titanium; implants, endodontics treatment, treatment; composite. It found with use composites, amalgams, glass-ionomers, materials for root canal filling/rinsing, orthodontic braces (made various metal alloys), titanium or whitening agents can disturb affecting antioxidant barrier increasing damage salivary proteins, lipids, DNA. Abnormal saliva secretion/composition observed patients course OS. suggested addition antioxidants therapy applied during could protect patient against harmful effects

Язык: Английский

---

Meta-Analysis of Assessment of Total Oxidative Stress and Total Antioxidant Capacity in Patients with Periodontitis

Disease Markers, Год журнала: 2023, Номер 2023, С. 1 - 17

Опубликована: Окт. 30, 2023

Background. Periodontitis is intricately linked to oxidative stress-antioxidant (redox) imbalance. The antioxidant system scavenges the oxygen free radicals in biological fluids patients with periodontitis. However, little still known about mediated stress and reductive ability of system. Thus, present meta-analysis aims quantitatively review literature that assessed marker total (TOS) capacity (TAC) various Methodology. Electronic databases were searched for studies TOS TAC levels samples Results. From 1,812 articles identified, 1,754 excluded based on title abstract screening due irrelevance topic interest. A full-text assessment remaining 58 led selection 42 satisfied inclusion criteria. Of these, only 24 had consistent data quantitative analysis. periodontitis group displayed significantly elevated ( p<0.05 ) serum, gingival crevicular fluid (GCF), saliva evaluated. In contrast, exhibited attenuated p<0.01 compared healthy controls plasma, GCF evaluated, which was insignificant salivary p=0.433 ). At same time, insignificantly after periodontal therapy p=0.130 Conclusions. showed higher lower periodontitis, reflecting level than control group. Clinical Relevance. Scientific rationale study: imbalance between oxidants antioxidants (oxidative (OS)) plays a critical role onset progression periodontitis; relationship OS-related biomarkers regional systemic helps us evaluate disease progression. OS biomarker can be used assess therapeutic efficacy.

Язык: Английский

---

Microbiological and Metabolomic Analysis of Biomarkers for Grades A and B in Stage II Periodontitis

Inflammation, Год журнала: 2025, Номер unknown

Опубликована: Фев. 27, 2025

Periodontitis is a chronic inflammatory disease characterized by inflammation of the periodontal soft tissues and loss alveolar bone. In oral environment, subgingival microorganisms salivary metabolites reflect host's health status. This study aimed to understand periodontitis severity progression rate analyzing microflora identify potential biomarkers. Fifty-three volunteers with stage II were graded using bone (%)/age index into two grades: 33 in grade A (< 0.25) 20 B (0.25–1.00). Using case–control study, simultaneously analyzed biomarkers associated periodontitis. The red complex, orange Campylobacter spp., uncultured Candidatus Saccharibacteria such as 5-Aminovaleric acid, N1-Acetylspermine showed significant positive correlation clinical parameters. Furthermore, we identified four differential (DL-Leucineamide, Dodecanedioic L-Tyrosine methyl ester Phenylpyruvic acid) that may serve for predicting progression. These results complex significantly correlated influenced changes metabolites. Additionally, indicating predominantly amino acid derivatives, confirming interactions between exacerbate development.

Язык: Английский

---

Antioxidant Mechanisms of the Protective Action of Selenase in Experimental Chronic Generalized Periodontitis

Current Issues in Molecular Biology, Год журнала: 2025, Номер 47(3), С. 186 - 186

Опубликована: Март 12, 2025

Inflammatory periodontal diseases, despite all the efforts of modern dentistry, remain an important predictor tooth loss worldwide. Oxidative stress plays a crucial role in pathogenesis periodontitis, making use antioxidants attractive option for its treatment. Our attention was drawn to selenium compound Selenase as antioxidant therapeutic agent. In this study, we modeled calcium-deficient prooxidant chronic generalized periodontitis (CGP) model white non-linear rats. Then, after 14 days, (50 μg/kg) and Mexidol (250 mg/kg) were administered intragastrically. Blood samples from animals analyzed using ELISA biochemical methods determine Cu-Zn SOD, nitrotyrosine, GPX-4, iNOS, NOx, GSH, GSSG levels. The CGP led typical clinical signs including hyperemia, edema, gingival pocket formation, bleeding, mobility, well increase molecular–biochemical markers nitrosative reduction endogenous blood. resulted decrease manifestations CGP, reduced NOx levels, SOD GPX-4 compared control group (p < 0.05). had less pronounced effect on these

Язык: Английский

---

The salivary metabolomics analyses reveal the variable metabolites in distinct staging of periodontitis

BMC Oral Health, Год журнала: 2025, Номер 25(1)

Опубликована: Апрель 3, 2025

Saliva, which is a critical component of the oral ecosystem, undergoes dynamic changes, particularly during onset and progression periodontitis. This study used Gas Chromatography-Mass Spectrometry (GC-MS), reliable high-throughput tool for metabolomic analysis, to detect salivary metabolic shifts across various stages periodontitis (T1-T4). We compared differential changes in metabolites between HC T1 groups, T2 T3 T4 groups. By analysing saliva samples from 116 individuals-10 healthy controls (HC) 106 patients with (22 individuals) (28 individuals), we identified including Glucose, 3-Aminobutanoic Acid, N-(1-Cyclopropylethyl) Aniline, Methylmalonic Acid. Compared HC, exhibited progressive concentration correlating severity disease. Furthermore, KEGG pathway analysis was elucidate pathways involved development Our findings demonstrate potential as biomarkers monitoring offer valuable insights into its pathogenesis therapeutic targets.

Язык: Английский

---