Hydrogel-Based Therapies for Ischemic and Hemorrhagic Stroke: A Comprehensive Review
Gels,
Год журнала:
2024,
Номер
10(7), С. 476 - 476
Опубликована: Июль 18, 2024
Stroke
remains
the
second
leading
cause
of
death
and
a
major
disability
worldwide,
significantly
impacting
individuals,
families,
healthcare
systems.
This
neurological
emergency
can
be
triggered
by
ischemic
events,
including
small
vessel
arteriolosclerosis,
cardioembolism,
large
artery
atherothromboembolism,
as
well
hemorrhagic
incidents
resulting
from
macrovascular
lesions,
venous
sinus
thrombosis,
or
vascular
malformations,
to
significant
neuronal
damage.
The
resultant
motor
impairment,
cognitive
dysfunction,
emotional
disturbances
underscore
urgent
need
for
effective
therapeutic
interventions.
Recent
advancements
in
biomaterials,
particularly
hydrogels,
offer
promising
new
avenues
stroke
management.
Hydrogels,
composed
three-dimensional
networks
hydrophilic
polymers,
are
notable
their
ability
absorb
retain
substantial
amounts
water.
Commonly
used
polymers
hydrogel
formulations
include
natural
like
alginate,
chitosan,
collagen,
synthetic
such
polyethylene
glycol
(PEG),
polyvinyl
alcohol
(PVA),
polyacrylamide.
Their
customizable
characteristics—such
porosity,
swelling
behavior,
mechanical
strength,
degradation
rates—make
hydrogels
ideal
biomedical
applications,
drug
delivery,
cell
tissue
engineering,
controlled
release
agents.
review
comprehensively
explores
hydrogel-based
approaches
both
therapy,
elucidating
mechanisms
which
provide
neuroprotection.
It
covers
application
delivery
systems,
role
reducing
inflammation
secondary
injury,
potential
support
neurogenesis
angiogenesis.
also
discusses
current
technology
challenges
translating
these
innovations
research
into
clinical
practice.
Additionally,
it
emphasizes
limited
number
trials
utilizing
therapies
addresses
associated
limitations
constraints,
underscoring
further
this
field.
Язык: Английский
Administration of Human-Derived Mesenchymal Stem Cells Activates Locally Stimulated Endogenous Neural Progenitors and Reduces Neurological Dysfunction in Mice after Ischemic Stroke
Cells,
Год журнала:
2024,
Номер
13(11), С. 939 - 939
Опубликована: Май 29, 2024
Increasing
evidence
shows
that
the
administration
of
mesenchymal
stem
cells
(MSCs)
is
a
promising
option
for
various
brain
diseases,
including
ischemic
stroke.
Studies
have
demonstrated
MSC
transplantation
after
stroke
provides
beneficial
effects,
such
as
neural
regeneration,
partially
by
activating
endogenous
stem/progenitor
(NSPCs)
in
conventional
neurogenic
zones,
subventricular
and
subgranular
zones.
However,
whether
regulates
fate
injury-induced
NSPCs
(iNSPCs)
regionally
activated
at
injured
regions
remains
unclear.
Therefore,
mice
were
subjected
to
stroke,
mCherry-labeled
human
MSCs
(h-MSCs)
transplanted
around
sites
nestin-GFP
transgenic
mice.
Immunohistochemistry
sections
revealed
many
GFP
Язык: Английский
Stem cell-derived exosomes for ischemic stroke: a conventional and network meta-analysis based on animal models
Frontiers in Pharmacology,
Год журнала:
2024,
Номер
15
Опубликована: Окт. 23, 2024
Objective
We
aimed
to
evaluate
the
efficacy
of
stem
cell-derived
exosomes
for
treating
ischemic
stroke
and
screen
optimal
administration
strategy.
Methods
searched
PubMed,
Web
Science,
Embase,
Cochrane
Library,
Scopus
databases
relevant
studies
published
from
their
inception
31
December
2023.
Conventional
network
meta-analyses
routes
administration,
types,
immune
compatibility
were
performed
using
cerebral
infarct
volume
(%)
modified
neurological
severity
score
(mNSS)
as
outcome
indicators.
Results
A
total
38
randomized
controlled
animal
experiments
included.
meta-analysis
showed
that
compared
with
negative
control
group:
intravenous
significantly
reduced
mNSS;
intranasal
(%);
intracerebral
mNSS.
Adipose-derived
mesenchymal
(ADSC-Exos),
bone
marrow
(BMSC-Exos),
dental
pulp
(DPSC-Exos)
neural
(NSC-Exos)
Endothelial
progenitor
(EPC-Exos),
embryonic
(ESC-Exos),
induced
pluripotent
(iPSC-Exos)
(NPC-Exos)
Umbilical
cord
(UCMSC-Exos)
there
was
no
significant
difference
between
urogenital
(USC-Exos)
controls.
Engineered
had
better
than
unmodified
exosomes.
Both
allogeneic
xenogeneic
The
best
route
reducing
Among
10
types
administered
intravenously,
BMSC-Exos
type
Allogeneic
in
(%),
whereas
Conclusion
This
meta-analysis,
by
integrating
available
evidence,
revealed
is
are
exosome
type,
have
mNSS,
which
can
provide
options
preclinical
studies.
In
future,
more
high-quality
experiments,
especially
direct
comparative
needed
determine
strategy
stroke.
Systematic
Review
Registration:
https://www.crd.york.ac.uk/PROSPERO/display_record.php?ID=CRD42024497333
,
PROSPERO,
CRD42024497333
Язык: Английский
Pharmacological and stem cell therapy of stroke in animal models: Do they accurately reflect the response of humans?
Experimental Neurology,
Год журнала:
2024,
Номер
376, С. 114753 - 114753
Опубликована: Март 14, 2024
Cerebrovascular
diseases
are
the
second
leading
cause
of
death
worldwide.
Despite
significant
research
investment,
only
available
therapeutic
options
mechanical
thrombectomy
and
tissue
plasminogen
activator
thrombolysis.
None
more
than
a
thousand
drugs
tested
on
animal
models
have
proven
successful
in
human
clinical
trials.
Several
factors
contribute
to
this
poor
translation
data
from
stroke-related
stroke
patients.
Firstly,
our
understanding
molecular
cellular
processes
involved
recovering
an
ischemic
is
severely
limited.
Secondly,
although
risk
particularly
high
among
older
patients
with
comorbidities,
most
young,
healthy
animals
controlled
laboratory
conditions.
Furthermore,
models,
tracking
post-stroke
recovery
typically
spans
3
28
days,
occasional
extensions
60
whereas
extended
complex
process.
Thirdly,
young
often
exhibit
considerably
higher
rate
spontaneous
compared
humans
following
stroke.
Fourth,
very
limited
number
utilized
for
each
condition,
including
control
groups.
Another
contributing
factor
much
smaller
beneficial
effects
that
positive
outcomes
numerous
studies
readily
accepted
results
reported
trials
do
not
show
clear
benefit
patient.
Useful
recommendations
conducting
experiments
increased
chances
translatability
humans,
been
issued
by
both
STEPS
investigative
team
STAIR
committee.
However,
largely,
due
economic
factors,
these
largely
ignored.
one
might
attribute
overall
failures
predicting
subsequently
developing
effective
acute
therapies
beyond
thrombolysis
potential
design
deficiencies
Язык: Английский
Comparative study of the efficacy of intra-arterial and intravenous transplantation of human induced pluripotent stem cells-derived neural progenitor cells in experimental stroke
PeerJ,
Год журнала:
2023,
Номер
11, С. e16358 - e16358
Опубликована: Ноя. 9, 2023
Cell
therapy
using
neural
progenitor
cells
(NPCs)
is
a
promising
approach
for
ischemic
stroke
treatment
according
to
the
results
of
multiple
preclinical
studies
in
animal
models.
In
vast
majority
conducted
studies,
therapeutic
efficacy
NPCs
was
estimated
after
intracerebral
transplantation,
while
information
effectiveness
systemic
administration
limited.
Nowadays,
several
clinical
trials
aimed
estimate
safety
and
transplantation
patients
were
also
conducted.
these
transplanted
intracerebrally
subacute/chronic
phase
stroke.
The
confirmed
approach,
however,
degree
functional
improvement
(the
primary
endpoint)
not
sufficient
studies.
Therefore,
more
are
needed
order
investigate
optimal
parameters,
especially
timing
cell
onset.
This
study
evaluate
effects
intra-arterial
(IA)
intravenous
(IV)
derived
from
induced
pluripotent
stem
(iNPCs)
acute
experimental
rats.
Induced
chosen
as
source
this
technology
perspective,
has
no
ethical
concerns
provides
access
personalized
medicine.Human
iNPCs
IA
or
IV
into
male
Wistar
rats
24
h
middle
cerebral
artery
occlusion
modeling.
Therapeutic
monitored
14
days
evaluated
comparison
with
transplantation-free
control
group.
Additionally,
distribution
brain
assessed.The
obtained
show
that
both
routes
(IV
IA)
significantly
reduced
mortality
improved
neurological
deficit
animals
compared
At
same
time,
MRI
data,
only
led
faster
prominent
reduction
volume.
After
administration,
transiently
trapped
detected
on
day
7
transplantation.
case
injection,
visualized
brain.
data
demonstrated
human
can
be
strategy.
Язык: Английский
Estimation of the Ischemic Lesion in the Experimental Stroke Studies Using Magnetic Resonance Imaging (Review)
Bulletin of Experimental Biology and Medicine,
Год журнала:
2024,
Номер
176(5), С. 649 - 657
Опубликована: Март 1, 2024
Язык: Английский
Comparative Study of the Protective and Neurotrophic Effects of Neuronal and Glial Progenitor Cells-Derived Conditioned Media in a Model of Glutamate Toxicity In Vitro
Biomolecules,
Год журнала:
2023,
Номер
13(12), С. 1784 - 1784
Опубликована: Дек. 13, 2023
Cell
therapy
represents
a
promising
approach
to
the
treatment
of
neurological
diseases,
offering
potential
benefits
not
only
by
cell
replacement
but
also
through
paracrine
secretory
activities.
However,
this
includes
number
limiting
factors,
primarily
related
safety.
The
use
conditioned
stem
media
can
serve
as
an
equivalent
while
avoiding
its
disadvantages.
present
study
was
comparative
investigation
antioxidant,
neuroprotective
and
neurotrophic
effects
obtained
from
neuronal
glial
progenitor
cells
(NPC-CM
GPC-CM)
on
PC12
line
in
vitro.
Neuronal
were
iPSCs
directed
differentiation
using
small
molecules.
GPC-CM
reduced
apoptosis,
ROS
levels
increased
viability,
expressions
antioxidant
response
genes
HMOX1
NFE2L2
model
glutamate-induced
oxidative
stress.
effect
evidenced
change
morphology
pheochromocytoma
neuron-like
phenotype.
Moreover,
neurite
outgrowth,
expression
GAP43,
TUBB3,
MAP2,
SYN1
corresponding
MAP2
TUBB3
proteins.
Treatment
with
NPC-CM
showed
moderate
antiapoptotic
improved
viability.
This
demonstrated
application
CM
field
regenerative
medicine.
Язык: Английский
Experience of using intracranial introduction of endothelin-1 for inducing ischemic brain damage in rats to evaluate cell therapy efficacy
Regional blood circulation and microcirculation,
Год журнала:
2024,
Номер
23(3), С. 64 - 69
Опубликована: Окт. 10, 2024
Introduction
.
Ischemic
stroke
is
one
of
the
leading
cause
disability
and
death.
One
promising
therapeutic
methods
cell
therapy.
The
choice
an
appropriate
experimental
model
great
importance
for
studying
restorative
effect
therapy
in
laboratory.
An
using
endothelin-1
allows
to
induce
damage
cortical
subcortical
brain
structures,
while
being
relatively
easy
perform
less
traumatic.
Aim
To
assess
effectiveness
ischemic
reproduced
analyze
results
general
cellular
on
recovery
processes.
Materials
Methods
Male
Wistar-Kyoto
rats
were
divided
into
groups:
sham
operation
(n=4),
ischemia
control
(n=9),
(n=8).
Damage
was
assessed
by
morphometry
immunohistochemistry
with
antibodies
against
NeuN,
GFAP,
nestin,
vimentin.
Results
It
has
been
established
that
this
causes
area
close
injection
site
characteristic
reactions.
Assessing
size
showed
a
large
within-group
variability,
which
did
not
allow
us
confirm
expected
Conclusion
endothelin
cerebral
suitable
reactions
develop
after
stroke,
but
significant
variability
volume
does
it
be
recommended
quantitative
assessment
effects
Язык: Английский
Stem Cell Therapy for Stroke: Mechanisms and Functional Recovery
IntechOpen eBooks,
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 4, 2024
Stem
cell
therapy
has
emerged
as
a
promising
approach
for
enhancing
recovery
following
stroke,
leading
cause
of
disability
worldwide.
This
chapter
provides
comprehensive
overview
the
mechanisms
through
which
stem
exerts
its
effects
on
brain
repair
and
functional
recovery.
We
explore
several
types
cells,
including
mesenchymal
neural
induced
pluripotent
their
potential
to
promote
neurogenesis,
angiogenesis,
synaptic
plasticity.
Additionally,
we
discuss
paracrine
highlighting
role
in
modulating
inflammation
reducing
apoptosis.
Preclinical
studies
clinical
trials
are
reviewed
evaluate
efficacy
safety
different
therapies.
Furthermore,
address
challenges
limitations
currently
hindering
translation
into
routine
practice,
such
optimal
delivery
methods,
immune
rejection,
ethical
considerations.
By
elucidating
underlying
summarizing
latest
research
advancements,
this
aims
provide
valuable
insights
viable
treatment
strategy
stroke
patients,
contributing
improved
quality
life.
Язык: Английский