On-chip recapitulation of the tumor microenvironment: A decade of progress
Biomaterials,
Год журнала:
2024,
Номер
306, С. 122482 - 122482
Опубликована: Янв. 22, 2024
One
of
the
hurdles
to
development
new
anticancer
therapies
is
lack
in
vitro
models
which
faithfully
reproduce
vivo
tumor
microenvironment
(TME).
Understanding
dynamic
relationships
between
components
TME
a
controllable,
scalable,
and
reliable
setting
would
indeed
support
discovery
biological
targets
impacting
cancer
diagnosis
therapy.
Cancer
research
increasingly
shifting
from
traditional
two-dimensional
(2D)
cell
culture
toward
three-dimensional
(3D)
models,
have
been
demonstrated
increase
significance
predictive
value
data.
In
this
scenario,
microphysiological
systems
(also
known
as
Organs-on-Chip)
emerged
relevant
technological
platform
enabling
more
investigation
cell-cell
cell-ECM
interplay
cancer,
attracting
significant
effort
last
years.
This
review
illustrates
one
decade
progress
field
tumor-microenvironment-on-chip
(TMOC)
approaches,
exploiting
either
cell-laden
microfluidic
chambers
or
confined
spheroids
model
TME.
TMOCs
designed
recapitulate
several
aspects
TME,
including
cells,
tumor-associated
stroma,
immune
system,
vascular
component.
Significantly,
aspect
has
for
its
pivotal
role
orchestrating
cellular
interactions
modulating
drug
pharmacokinetics
on-chip.
A
further
advancement
represented
by
integration
into
multi-organ
systems,
with
final
aim
follow
metastatic
cascade
target
organs
study
effects
chemotherapies
at
systemic
level.
We
highlight
that
increased
degree
complexity
achieved
most
advanced
TMOC
enabled
scientists
shed
light
on
microenvironmental
factors
progression,
cascade,
response
drugs.
Язык: Английский
An Insight on Microfluidic Organ-on-a-Chip Models for PM2.5-Induced Pulmonary Complications
ACS Omega,
Год журнала:
2024,
Номер
9(12), С. 13534 - 13555
Опубликована: Март 7, 2024
Pulmonary
diseases
like
asthma,
chronic
obstructive
pulmonary
disorder,
lung
fibrosis,
and
cancer
pose
a
significant
burden
to
global
human
health.
Many
of
these
complications
arise
as
result
exposure
particulate
matter
(PM),
which
has
been
examined
in
several
preclinical
clinical
trials
for
its
effect
on
respiratory
diseases.
Particulate
size
less
than
2.5
μm
(PM2.5)
known
inflict
unforeseen
repercussions,
although
data
from
epidemiological
studies
back
this
are
pending.
Conventionally
utilized
two-dimensional
(2D)
cell
culture
animal
models
have
provided
insufficient
benefits
emulating
the
vivo
physiological
pathological
conditions.
Three-dimensional
(3D)
structural
models,
including
organ-on-a-chip
experienced
developmental
upsurge
recent
times.
Lung-on-a-chip
potential
simulate
specific
features
lungs.
With
advancement
technology,
an
emerging
advanced
technique
termed
microfluidic
developed
with
aim
identifying
complexity
cellular
microenvironment
body.
In
present
Review,
role
lung-on-a-chip
modeling
reproducing
explored,
emphasis
PM2.5-induced
complications.
Язык: Английский
Organ-on-a-chip: future of female reproductive pathophysiological models
Journal of Nanobiotechnology,
Год журнала:
2024,
Номер
22(1)
Опубликована: Июль 31, 2024
Abstract
The
female
reproductive
system
comprises
the
internal
and
external
genitalia,
which
communicate
through
intricate
endocrine
pathways.
Besides
secreting
hormones
that
maintain
secondary
sexual
characteristics,
it
also
produces
follicles
offspring.
However,
in
vitro
systems
have
been
very
limited
recapitulating
specific
anatomy
pathophysiology
of
women.
Organ-on-a-chip
technology,
based
on
microfluidics,
can
better
simulate
cellular
microenvironment
vivo,
opening
a
new
field
for
basic
clinical
research
diseases.
This
technology
not
only
reconstruct
organ
structure
but
emulate
function
as
much
possible.
precisely
controlled
fluidic
provided
by
microfluidics
vividly
mimics
complex
hormone
crosstalk
among
various
organs
system,
making
powerful
preclinical
tool
future
pathophysiological
models
system.
Here,
we
review
application
organ-on-a-chip
platforms
systems,
focusing
latest
progress
developing
reproduce
physiological
functions
or
disease
features
tissues,
highlighting
challenges
directions
this
field.
Язык: Английский
Construction of in vitro liver-on-a-chip models and application progress
BioMedical Engineering OnLine,
Год журнала:
2024,
Номер
23(1)
Опубликована: Март 15, 2024
Abstract
The
liver
is
the
largest
internal
organ
of
human
body.
It
has
a
complex
structure
and
function
plays
vital
role
in
drug
metabolism.
In
recent
decades,
extensive
research
aimed
to
develop
vitro
models
that
can
simulate
demonstrate
changes
physiological
pathological
environment
liver.
Animal
cell
are
common,
but
data
obtained
from
animal
lack
relevance
when
applied
humans,
while
have
limited
predictive
ability
for
metabolism
toxicity
humans.
Recent
advancements
tissue
engineering,
biomaterials,
chip
technology,
3D
bioprinting
provided
opportunities
further
models.
Among
them,
liver-on-a-Chip
(LOC)
technology
made
significant
achievements
reproducing
vivo
behavior,
microenvironment,
cells
organs.
this
review,
we
discuss
development
LOC
its
progress
diseases,
hepatotoxicity
tests,
screening,
as
well
combinations.
First,
review
Then,
introduce
including
general
concepts,
preparation
materials,
methods.
Finally,
application
disease
modeling,
combinations,
future
challenges
directions
LOC.
Язык: Английский
Advancing diagnostics and disease modeling: current concepts in biofabrication of soft microfluidic systems
In vitro models,
Год журнала:
2024,
Номер
3(2-3), С. 139 - 150
Опубликована: Июнь 4, 2024
Abstract
Soft
microfluidic
systems
play
a
pivotal
role
in
personalized
medicine,
particularly
vitro
diagnostics
tools
and
disease
modeling.
These
offer
unprecedented
precision
versatility,
enabling
the
creation
of
intricate
three-dimensional
(3D)
tissue
models
that
can
closely
emulate
both
physiological
pathophysiological
conditions.
By
leveraging
innovative
biomaterials
bioinks,
soft
circumvent
current
limitations
involving
use
polydimethylsiloxane
(PDMS),
thus
facilitating
development
customizable
capable
sustaining
functions
encapsulated
cells
mimicking
complex
biological
microenvironments.
The
integration
lab-on-a-chip
technologies
with
nanodevices
further
enhances
models,
paving
way
for
tailored
therapeutic
strategies.
research
concepts
underscore
transformative
potential
systems,
exemplified
by
recent
breakthroughs
lithography
3D
(bio)printing.
Novel
applications,
such
as
multi-layered
tissues-on-chips
skin-on-a-chip
devices,
demonstrate
significant
advancements
modeling
medicine.
However,
exploration
is
warranted
to
address
challenges
replicating
structures
while
ensuring
scalability
reproducibility.
This
promises
drive
innovation
biomedical
healthcare,
offering
new
insights
solutions
medical
unmet
needs.
Язык: Английский
Tissue chips as headway model and incitement technology
Synthetic and Systems Biotechnology,
Год журнала:
2024,
Номер
10(1), С. 86 - 101
Опубликована: Авг. 30, 2024
Tissue
on
a
chip
or
organ-on-chip
(OOC)
is
technology
that's
dignified
to
form
transformation
in
drug
discovery
through
the
use
of
advanced
platforms.
These
are
3D
Язык: Английский
Design and simulation study of organ-on-a-chip
Elsevier eBooks,
Год журнала:
2024,
Номер
unknown, С. 149 - 174
Опубликована: Янв. 1, 2024
Язык: Английский
Engineering microfluidic devices to mimic signaling cascades in continuous-flow cell culture as multiorgan microphysiological systems
Biochemical Engineering Journal,
Год журнала:
2024,
Номер
211, С. 109475 - 109475
Опубликована: Авг. 30, 2024
Язык: Английский
Cancer-on-chip: a breakthrough organ-on-a-chip technology in cancer cell modeling
Medical & Biological Engineering & Computing,
Год журнала:
2024,
Номер
unknown
Опубликована: Окт. 14, 2024
Abstract
Cancer
remains
one
of
the
leading
causes
death
worldwide.
The
unclear
molecular
mechanisms
and
complex
in
vivo
microenvironment
tumors
make
it
difficult
to
clarify
nature
cancer
develop
effective
treatments.
Therefore,
development
new
methods
effectively
treat
is
urgently
needed
great
importance.
Organ-on-a-chip
(OoC)
systems
could
be
breakthrough
technology
sought
by
pharmaceutical
industry
address
ever-increasing
research
costs.
past
decade
has
seen
significant
advances
spatial
modeling
therapeutics
related
OoC
technology,
improving
physiological
exposition
criteria.
This
article
aims
summarize
latest
achievements
results
cell
treatment
simulated
a
3D
using
technology.
To
this
end,
we
will
first
discuss
system
detail
then
demonstrate
findings
study
Ooc
how
technique
can
potentially
optimize
better
tumor.
prospects
cells
their
advantages
limitations
are
also
among
other
points
discussed
study.
Graphical
Язык: Английский
Fluoropolymer Functionalization of Organ-on-Chip Platform Increases Detection Sensitivity for Cannabinoids
Biosensors,
Год журнала:
2023,
Номер
13(8), С. 779 - 779
Опубликована: Авг. 1, 2023
Microfluidic
technology
is
applied
across
various
research
areas
including
organ-on-chip
(OOC)
systems.
The
main
material
used
for
microfluidics
polydimethylsiloxane
(PDMS),
a
silicone
elastomer
that
biocompatible,
transparent,
and
easy
to
use
OOC
systems
with
well-defined
microstructures.
However,
PDMS-based
can
absorb
hydrophobic
small
molecules,
making
it
difficult
erroneous
make
quantitative
analytical
assessments
such
compounds.
In
this
paper,
we
explore
the
of
synthetic
fluoropolymer,
poly(4,5-difluoro-2,2-bis(trifluoromethyl)-1,3-dioxole-co-tetrafluoroethylene)
(Teflon™
AF
2400),
excellent
"non-stick"
properties
functionalize
Cannabinoids,
cannabidiol
(CBD),
are
classes
compounds
great
potential
treatment
anxiety,
depression,
pain,
cancer.
By
using
CBD
as
testing
compound,
examined
systematically
quantified
absorption
into
PDMS
by
means
an
LC-MS/MS
analysis.
comparison
unmodified
microchannels,
increase
approximately
30×
in
signal
was
detected
fluoropolymer
surface
modification
after
3
h
static
incubation.
Under
perfusion
conditions,
observed
nearly
15×
signals
from
surface-modified
microchannels
than
microchannels.
Furthermore,
also
demonstrated
fluoropolymer-modified
compatible
culturing
hCMEC/D3
endothelial
cells
experiments.
Язык: Английский