Cardiology in the Young,
Год журнала:
2024,
Номер
35(2), С. 324 - 331
Опубликована: Ноя. 29, 2024
The
microRNA-200
family
plays
a
key
role
in
inflammatory
and
vascular
processes,
making
it
relevant
target
for
Kawasaki
disease,
vasculitis
with
coronary
complications.
This
study
aimed
to
evaluate
the
diagnostic
potential
of
urinary
exosomal
members
disease
patients.
Urine
samples
from
15
patients
healthy
controls
underwent
total
exosome
isolation
high-throughput
sequencing.
Differential
expression
was
validated
using
quantitative
real-time
polymerase
chain
reaction.
Diagnostic
assessed
via
receiver
operating
characteristic
analysis,
correlations
clinical
parameters
were
evaluated
Spearman
correlation.
High-throughput
sequencing
identified
upregulation
microRNA-429,
microRNA-200b-3p/5p,
microRNA-141-3p,
microRNA-200a-3p/5p,
microRNA-200c-3p
We
confirmed
significant
analysis
showing
high
these
microRNAs
(area
under
curves
0.844,
0.791,
0.942,
0.842,
0.898,
respectively)
combined
yielding
perfect
area
curve
1.000.
MicroRNA-141
microRNA-200c-3p/5p,
however,
showed
no
value.
MicroRNA-200a-3p
microRNA-200a-5p
positively
correlated
white
blood
cells,
platelet
counts,
C-reactive
protein,
while
microRNA-200b-3p
microRNA-429
erythrocyte
sedimentation
rate,
protein.
microRNA-200b-5p
moderate
counts
rate.
Urinary
members,
especially
demonstrate
strong
correlating
markers.
microRNA-200c
did
not
utility.
Abstract
The
RNA
found
in
the
circular
system
is
known
as
extracellular
(exRNA).
This
kind
of
has
been
to
play
a
biological
role
similar
that
messenger.
They
can
be
used
indicators
disease
status
or
physiological
health
an
organism.
A
large
number
RNA‐based
biomaterials
have
developed
by
simulating
function
and
structure
natural
molecules.
structural
programmability
provides
spur
for
scientists
pioneer
new
approaches
detection
prevention.
Nevertheless,
link
between
exRNA
design
not
fully
understood.
Understanding
will
contribute
clinical
translation
this
novel
biotechnology.
present
review
discusses
research
progress
associated
with
their
derivatives
bridge
gap
biomaterials.
Viruses,
Год журнала:
2024,
Номер
16(11), С. 1785 - 1785
Опубликована: Ноя. 17, 2024
Extracellular
vesicles
(EVs)
are
bilayer
released
by
cells
in
the
microenvironment
of
liver
including
parenchymal
and
non-parenchymal
cells.
They
third
important
mechanism
communications
between
cells,
besides
secretion
cytokines
chemokines
direct
cell-to-cell
contact.
The
aim
this
review
is
to
discuss
role
EVs
viral
disease,
as
there
increasing
evidence
that
transportation
proteins,
all
types
RNA,
particles
complete
virions
implicated
pathogenesis
both
cirrhosis
viral-related
hepatocellular
carcinoma.
biogenesis
discussed
their
diseases
presented.
Their
use
diagnostic
prognostic
biomarkers
also
analyzed.
Most
importantly,
significance
possible
novel
treatment
strategies
for
fibrosis
carcinoma
presented,
although
available
data
based
on
experimental
clinical
trials
have
not
been
reported.
Cardiology in the Young,
Год журнала:
2024,
Номер
35(2), С. 324 - 331
Опубликована: Ноя. 29, 2024
The
microRNA-200
family
plays
a
key
role
in
inflammatory
and
vascular
processes,
making
it
relevant
target
for
Kawasaki
disease,
vasculitis
with
coronary
complications.
This
study
aimed
to
evaluate
the
diagnostic
potential
of
urinary
exosomal
members
disease
patients.
Urine
samples
from
15
patients
healthy
controls
underwent
total
exosome
isolation
high-throughput
sequencing.
Differential
expression
was
validated
using
quantitative
real-time
polymerase
chain
reaction.
Diagnostic
assessed
via
receiver
operating
characteristic
analysis,
correlations
clinical
parameters
were
evaluated
Spearman
correlation.
High-throughput
sequencing
identified
upregulation
microRNA-429,
microRNA-200b-3p/5p,
microRNA-141-3p,
microRNA-200a-3p/5p,
microRNA-200c-3p
We
confirmed
significant
analysis
showing
high
these
microRNAs
(area
under
curves
0.844,
0.791,
0.942,
0.842,
0.898,
respectively)
combined
yielding
perfect
area
curve
1.000.
MicroRNA-141
microRNA-200c-3p/5p,
however,
showed
no
value.
MicroRNA-200a-3p
microRNA-200a-5p
positively
correlated
white
blood
cells,
platelet
counts,
C-reactive
protein,
while
microRNA-200b-3p
microRNA-429
erythrocyte
sedimentation
rate,
protein.
microRNA-200b-5p
moderate
counts
rate.
Urinary
members,
especially
demonstrate
strong
correlating
markers.
microRNA-200c
did
not
utility.
