Journal of Bioscience and Bioengineering, Год журнала: 2024, Номер unknown
Опубликована: Ноя. 1, 2024
Язык: Английский
Biomedicines, Год журнала: 2025, Номер 13(3), С. 534 - 534
Опубликована: Фев. 20, 2025
Background/Objective: Anti-tumor effects of plasma-activated medium (PAM) were demonstrated using various malignant tumors. However, the anti-tumor effect PAM on synovial sarcoma remains unclear. Therefore, we aimed to investigate and its underlying mechanisms, focusing quantitative analyses both intracellular reactive oxygen species (ROS) cell apoptosis. Methods: The human line HS-SY-II was used viability after treatment. We investigated side local injection in a xenograft murine model. Moreover, observed PAM-induced ROS accumulation apoptosis assessed involvement an scavenger. Results: significantly decreased cells compared with untreated Dulbecco’s modified Eagle medium. Local into model suppressed tumor growth, including volume (p < 0.001) weight = 0.031), without effects. Regarding induced significant 0.001). scavenger inhibited Conclusions: confirmed vitro vivo, as well absence mechanism suggested involve by accumulation. Considering clinical issues associated existing treatments sarcoma, is promising new option.
Язык: Английский
Процитировано
0
ACS Omega, Год журнала: 2025, Номер 10(12), С. 12109 - 12121
Опубликована: Март 6, 2025
Transitional cold atmospheric plasma (TCAP) represents a novel technique for generating remotely from primary source. It consists of partially nonthermal ionized gas mixture containing charged and neutral particles, photons, free radicals. In recent years, TCAP has attracted considerable attention in biomedical applications. order to evaluate colon cancer stem cells' (CCSCs) proliferation, apoptotic induction, inflammatory response, survival, was utilized both directly indirectly this study. Using argon helium gases, continuously delivered two stages during the experiment. For direct state, irradiated onto CCSCs 3 5 min. indirect technique, Matrigel treated with min before introduction cells. vitro assays demonstrated that exposure significantly reduced viability CCSCs; application had greater impacts than argon. Numerous investigations confirmed induction apoptosis, showing groups more cells altered cellular structures controls (****p < 0.0001). A substantial increase Bax/Bcl-2 ratio found by analyzing expression Bax Bcl-2 genes, indicating increased susceptibility apoptosis (*p = 0.0177 ***p 0.0004). The higher efficacy mode further highlighted marker analysis, which showed significant reduction interleukin-6 interleukin-8 TCAP-helium compared TCAP-argon (**p 0.0015 0.0007). Lastly, proliferation test, relies on Ki-67 expression, noteworthy decline all TCAP-treated groups, group exhibiting most robust impact 0.0014). Overall, findings highlight potential TCAP, particularly helium, as promising approach selectively targeting providing insights into its therapeutic mechanisms treatment. therefore, emerges unique strategy applications cell-targeted therapies.
Язык: Английский
Процитировано
0
Redox Biology, Год журнала: 2024, Номер 75, С. 103284 - 103284
Опубликована: Июль 23, 2024
Malignant melanoma is the most lethal form of skin cancer. As a promising anti-cancer agent, plasma-activated water (PAW) rich in reactive oxygen and nitrogen species (RONS) has shown significant potential for treatment. However, rapid decay RONS inefficient delivery PAW conventional injection methods limit its practical applications. To address this issue, here we report new approach production cryo-microneedles (PA-CMNs) patches using custom-designed plasma devices processes. Our innovation to incorporate into PA-CMNs that are fabricated fast cryogenic micro-molding method. It demonstrated can be easily inserted release slow thereby prolonging their bioactivity effectiveness. The insights effective treatment suggest mixture within prepared by custom-developed hybrid plasma-assisted configuration induces both ferroptosis apoptosis selectively kill tumor cells. A inhibition subcutaneous A375 growth was observed PA-CMNs-treated tumor-bearing nude mice without any signs systemic toxicity. based on may potentially open avenues broader range disease treatments.
Язык: Английский
Процитировано
2
Scientific Reports, Год журнала: 2024, Номер 14(1)
Опубликована: Март 18, 2024
Ewing's sarcoma (ES) is the second most common bone tumor in children and adolescents highly malignant. Although new chemotherapy has significantly improved survival rate for ES from about 10 to 75%, metastatic tumors remains around 30%. This treatment often associated with various side effects that contribute suffering of patients. Cold physical plasma (CPP), whether used alone or combination current chemotherapy, considered a promising adjunctive tool cancer treatment. study aims investigate synergistic CPP cytostatic chemotherapeutic agents are not part therapy. Two different cell lines, RD-ES A673, were treated determined IC
Язык: Английский
Процитировано
1
Biomedicines, Год журнала: 2024, Номер 12(5), С. 1029 - 1029
Опубликована: Май 7, 2024
Plasma applications in biomedicine represent a groundbreaking intersection between physics and life sciences, unveiling novel approaches to disease treatment tissue regeneration [...]
Язык: Английский
Процитировано
1
In Vivo, Год журнала: 2024, Номер 38(4), С. 1571 - 1578
Опубликована: Янв. 1, 2024
Background/Aim: Cold physical plasma (CPP) has emerged as an effective therapy in oncology by inducing cytotoxic effects various cancer cells, including chondrosarcoma (CS), Ewing's sarcoma (ES), and osteosarcoma (OS). The current study investigated the impact of CPP on cell motility CS (CAL-78), ES (A673), OS (U2-OS) lines, focusing actin cytoskeleton. Materials Methods: CASY Cell Counter Analyzer was used to proliferation determine optimal concentrations fetal calf serum maintain viability without stimulation proliferation. CellTiter-BlueCell assay bone cells. Radius migration. Staining for Deoxyribonuclease I, G-actin, F-actin Results: Reductions were observed across all lines following treatment. induced changes cytoskeleton, leading decreased motility. Conclusion: effectively reduces cells altering These findings underscore CPP's potential a therapeutic tool sarcomas highlight need further research this area.
Язык: Английский
Процитировано
1
International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(13), С. 6955 - 6955
Опубликована: Июнь 25, 2024
Chondrosarcoma (CS) is a rare malignant bone sarcoma that primarily affects cartilage cells in the femur and pelvis. While most subtypes exhibit slow growth with very good prognosis, some aggressive have poorer overall survival. CS known for its resistance to chemotherapy radiotherapy, leaving surgery as sole effective therapeutic option. Cold physical plasma (CPP) has been explored vitro potential therapy, demonstrating positive anti-tumor effects on cells. This study investigated synergistic of combining CPP cytostatics The chemotherapeutic agents cisplatin, doxorubicin, vincristine were applied two cell lines (CAL-78 SW1353). After determining their IC20 IC50, they combined both assess impact proliferation, viability, metabolism, apoptosis. approach significantly reduced proliferation viability while increasing apoptosis signals compared cytostatic therapy alone. combination drugs shows promise targeting chemoresistant cells, potentially improving prognosis patients clinical settings.
Язык: Английский
Процитировано
0
Journal of Bioscience and Bioengineering, Год журнала: 2024, Номер unknown
Опубликована: Ноя. 1, 2024
Язык: Английский
Процитировано
0