Biomedicines,
Год журнала:
2023,
Номер
11(10), С. 2743 - 2743
Опубликована: Окт. 10, 2023
One
of
the
major
goals
in
advancement
basic
cancer
research
focuses
on
development
new
anticancer
therapies.
To
understand
molecular
mechanisms
progression,
acquired
drug
resistance,
and
metastatic
process,
use
preclinical
vitro
models
that
faithfully
summarize
properties
tumor
patients
is
still
a
necessity.
The
represented
by
diverse
group
cell
clones,
recent
years,
to
reproduce
models,
monolayer
cultures
have
been
supplanted
patient-derived
xenograft
(PDX)
cultured
organoids
derived
from
patient
(PDO).
These
proved
indispensable
for
study
microenvironment
(TME)
its
interaction
with
cells.
Prostate
(PCa)
most
common
neoplasia
men
world.
It
characterized
genomic
instability
resistance
conventional
Despite
advances
diagnosis
treatment,
PCa
remains
leading
cause
death.
Here,
we
review
studies
last
10
years
as
number
papers
growing
very
fast
field.
We
also
discuss
discovered
limitations
challenges
using
organoid
culture
system
PDXs
studying
prostate
phenotype,
performing
testing,
developing
Nanomaterials,
Год журнала:
2024,
Номер
14(6), С. 531 - 531
Опубликована: Март 16, 2024
Biomimetic
scaffolds
imitate
native
tissue
and
can
take
a
multidimensional
form.
They
are
biocompatible
influence
cellular
metabolism,
making
them
attractive
bioengineering
platforms.
The
use
of
biomimetic
adds
complexity
to
traditional
cell
cultivation
methods.
most
commonly
used
technique
involves
cultivating
cells
on
flat
surface
in
two-dimensional
format
due
its
simplicity.
A
three-dimensional
(3D)
provide
microenvironment
for
surrounding
cells.
There
two
main
techniques
obtaining
3D
structures
based
the
presence
scaffolding.
Scaffold-free
consist
spheroid
technologies.
Meanwhile,
scaffold
contain
organoids
all
constructs
that
various
types
scaffolds,
ranging
from
decellularized
extracellular
matrix
(dECM)
through
hydrogels
one
extensively
studied
forms
potential
culture
up
4D
bioprinted
biomaterials.
bioprinting
is
important
create
scaffolds.
versatility
this
allows
many
different
inks,
mainly
hydrogels,
as
well
inorganic
substances.
Increasing
amounts
data
evidence
vast
usage
engineering
personalized
medicine,
with
area
application
being
regeneration
skin
musculoskeletal
systems.
Recent
papers
also
indicate
increasing
vivo
tests
products
which
further
strengthen
importance
branch
emphasize
need
extensive
research
safe
humansbiomimetic
tissues
organs.
In
review
article,
we
recent
advancements
field
preceded
by
an
overview
technologies
led
development
complex
type
culture.
ACS Applied Bio Materials,
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 28, 2025
Patients
and
healthcare
systems
face
significant
social
financial
challenges
due
to
the
increasing
number
of
individuals
with
chronic
external
internal
wounds
that
fail
heal.
The
complexity
healing
process
remains
a
serious
health
concern,
despite
effectiveness
conventional
wound
dressings
in
promoting
healing.
Recent
advancements
materials
science
fabrication
techniques
have
led
development
innovative
enhance
To
further
expedite
process,
novel
approaches
such
as
nanoparticles,
3D-printed
dressings,
biomolecule-infused
emerged,
along
cell-based
methods.
Additionally,
gene
therapy
technologies
are
being
harnessed
generate
stem
cell
derivatives
more
functional,
selective,
responsive
than
their
natural
counterparts.
This
review
highlights
potential
biomaterials,
3D
bioprinting,
gene-
therapies
However,
it
also
underscores
necessity
for
research
address
existing
integrate
these
strategies
into
standard
clinical
practice.
Senescence
is
a
complicated,
multi-factorial,
irreversible
cell
cycle
halt
that
has
tumor-suppressing
effect
in
addition
to
being
significant
factor
aging
and
neurological
diseases.
Damaged
DNA,
neuroinflammation,
oxidative
stress
disrupted
proteostasis
are
few
of
the
factors
cause
senescence.
triggered
by
DNA
damage
which
initiates
response.
The
response,
includes
formation
foci
containing
activated
H2AX,
key
cellular
senescence,
provoked
double
strand
break.
Oxidative
impairs
cognition,
inhibits
neurogenesis,
an
accelerated
effect.
Senescent
cells
generate
pro-inflammatory
mediators
known
as
senescence-associated
secretory
phenotype
(SASP).
These
cytokines
chemokines
have
impact
on
neuronal
death,
proliferation.
While
it
tempting
think
neurodegenerative
diseases
manifestations
this
review
will
present
information
brain
ageing
neurodegeneration
result
senescence
Cancers,
Год журнала:
2025,
Номер
17(1), С. 108 - 108
Опубликована: Янв. 1, 2025
Background:
Tumor
organoid
and
tumor-on-chip
(ToC)
platforms
replicate
aspects
of
the
anatomical
physiological
states
tumors.
They,
therefore,
serve
as
models
for
investigating
tumor
microenvironments,
metastasis,
immune
interactions,
especially
precision
drug
testing.
To
map
changing
research
diversity
focus
in
this
field,
we
performed
a
quality-controlled
text
analysis
categorized
academic
publications
clinical
studies.
Methods:
Previously,
collected
metadata
on
organoids
or
organ-on-chip
from
PubMed,
Web
Science,
Scopus,
EMBASE,
bioRxiv,
published
between
January
2011
June
2023.
Here,
selected
documents
corpus
that
were
computationally
determined
relevant
to
analyzed
them
using
an
in-house
algorithm.
Additionally,
ClinicalTrials.gov
studies
related
ToC
March
Results
Discussion:
From
3551
139
trials,
identified
55
24
classes
modeled
models,
respectively.
The
was
particularly
active
neural
hepatic/pancreatic
organoids,
well
gastrointestinal,
neural,
reproductive
models.
Comparative
with
cancer
statistics
showed
lung,
lymphatic,
cervical
tumors
under-represented
research.
Our
findings
also
illustrate
varied
topics,
including
physiology,
therapeutic
approaches,
cell
involvement,
analytical
techniques.
Mapping
geographically
highlighted
colorectal
Netherlands,
though
overall
specific
countries
did
not
reflect
regional
prevalence.
These
insights
only
current
landscape
but
indicate
potential
new
directions
model
Frontiers in Cell and Developmental Biology,
Год журнала:
2025,
Номер
12
Опубликована: Янв. 9, 2025
Liver
cancer
is
a
leading
cause
of
cancer-related
deaths
worldwide,
highlighting
the
need
for
innovative
approaches
to
understand
its
complex
biology
and
develop
effective
treatments.
While
traditional
in
vivo
animal
models
have
played
vital
role
liver
research,
ethical
concerns
demand
more
human-relevant
systems
driven
development
advanced
vitro
models.
Spheroids
organoids
emerged
as
powerful
tools
due
their
ability
replicate
tumor
microenvironment
facilitate
preclinical
drug
development.
are
simpler
3D
culture
that
partially
recreate
structure
cell
interactions.
They
can
be
used
penetration
studies
high-throughput
screening.
Organoids
derived
from
stem
cells
or
patient
tissues
accurately
emulate
complexity
functionality
tissue.
generated
pluripotent
adult
cells,
well
specimens,
providing
personalized
studying
behavior
responses.
retain
genetic
variability
original
offer
robust
platform
screening
treatment
strategies.
However,
both
spheroids
limitations,
such
absence
functional
vasculature
immune
components,
which
essential
growth
therapeutic
The
field
modeling
evolving,
with
ongoing
efforts
predictive
reflect
complexities
human
cancer.
By
integrating
these
tools,
researchers
gain
deeper
insights
into
accelerate
novel
Advances in Clinical Toxicology,
Год журнала:
2024,
Номер
9(1), С. 1 - 14
Опубликована: Янв. 1, 2024
Background:
Traditional
toxicity
testing
emphasizes
animal
models
with
growing
concerns
regarding
predictive
capacity,
throughput
and
ethics.
Rapid
innovation
surrounding
human
cell
platforms,
bioengineered
tissues,
omics
techniques
computational
tools
offers
more
modern
alternatives
aligned
expanding
knowledge
of
chemical
biological
pathways.
These
disruptive
approaches
promise
immense
potential
to
transform
next-generation
safety
assessment
drug
development
pipelines.
Purpose:
This
review
provides
clinical
researchers
an
updated,
comprehensive
perspective
across
evolving
areas
focus
in
new
methods
analysis
latest
advances
translational
context.
Main
Body:
We
survey
progress
two-
three-dimensional
cultures
recapitulating
tissue/organ
complexity
impossible
conventional
assays.
Complementing
this,
modeling
integrates
structure-activity
relationships,
physicochemical
properties
physiological
interactions
predict
pharmacokinetics
silico.
Expanding
model
organisms
add
further
dimensionality
demographic
relevance.
High-throughput
imaging
technologies
unravel
mechanisms
illuminate
biomarkers
undetectable
by
standard
measures.
Specialized
show
high
addressing
toxicodynamic
intricacies
within
disease
contexts
like
diabetes
NAFLD.
Evaluating
traditional
medicines
phytochemicals
likewise
represents
area
growth
well-suited
for
contemporary
platforms.
Future
outlook
weighs
remarkable
advantages
reducing
demands,
enabling
precision
toxicology
links
medicine
overhauling
core
risk
frameworks.
Conclusion:
intends
catalyze
discourse
on
strategic
optimization
priorities
roadmaps
towards
fully
unlocking
the
yet
still
emerging
public
health
these
poising
transformation
sciences
centered
human-focused
models.
Scientific Reports,
Год журнала:
2023,
Номер
13(1)
Опубликована: Окт. 23, 2023
While
chemotherapy
alone
or
in
combination
with
radiotherapy
and
surgery
are
important
modalities
the
treatment
of
colorectal
cancer,
their
widespread
use
is
not
paired
an
abundance
diagnostic
tools
to
match
individual
patients
most
effective
standard-of-care
chemo-
regimens.
Patient-derived
organoids
tumour-derived
structures
that
have
been
shown
retain
certain
aspects
tissue
origin.
We
present
here
a
systematic
review
studies
tested
performance
patient
derived
predict
effect
anti-cancer
therapies
for
chemotherapies,
targeted
drugs,
radiation
therapy,
we
found
overall
positive
predictive
value
68%
negative
78%
organoid
informed
treatment,
which
outperforms
response
rates
observed
empirically
guided
selection.
Animal Reproduction,
Год журнала:
2024,
Номер
21(1)
Опубликована: Янв. 1, 2024
In
vitro
cell
culture
is
a
well-established
technique
present
in
numerous
laboratories
diverse
areas.
reproduction,
gametes,
embryos,
and
reproductive
tissues,
such
as
the
ovary
endometrium,
can
be
cultured.
These
cultures
are
essential
for
embryo
development
studies,
understanding
signaling
pathways,
developing
drugs
diseases,
production
(IVP).
Although
many
systems
successful,
they
still
have
limitations
to
overcome.
Three-dimensional
(3D)
close
physiological
conditions,
allowing
greater
interaction
between
cells
with
surrounding
environment,
maintenance
of
cells'
natural
morphology,
expression
genes
proteins
vivo.
Additionally,
three-dimensional
stimulated
extracellular
matrix
generating
responses
due
mechanical
force
produced.
Different
techniques
used
perform
3D
systems,
hydrogel
matrix,
hanging
drop,
low
attachment
surface,
scaffold,
levitation,
liquid
marble,
printing.
demonstrate
satisfactory
results
follicle
culture,
from
pre-antral
antral
phase,
maintaining
follicular
increasing
rates
embryos.
Here,
we
review
some
different
their
applications
follicles
bringing
new
possibilities
future
assisted
reproduction.
