Development of a Novel Anti-CD44 Variant 6 Monoclonal Antibody C44Mab-9 for Multiple Applications against Colorectal Carcinomas

International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(4), С. 4007 - 4007

Опубликована: Фев. 16, 2023

CD44 is a cell surface glycoprotein, and its isoforms are produced by the alternative splicing with standard variant exons. The exon-containing (CD44v) overexpressed in carcinomas. CD44v6 one of CD44v, overexpression predicts poor prognosis colorectal cancer (CRC) patients. plays critical roles CRC adhesion, proliferation, stemness, invasiveness, chemoresistance. Therefore, promising target for diagnosis therapy CRC. In this study, we established anti-CD44 monoclonal antibodies (mAbs) immunizing mice CD44v3-10-overexpressed Chinese hamster ovary (CHO)-K1 cells. We then characterized them using enzyme-linked immunosorbent assay, flow cytometry, western blotting, immunohistochemistry. One clones (C44Mab-9; IgG1, kappa) reacted peptide 6-encoded region, indicating that C44Mab-9 recognizes CD44v6. Furthermore, CHO/CD44v3-10 cells or lines (COLO201 COLO205) cytometry. apparent dissociation constant (KD) CHO/CD44v3-10, COLO201, COLO205 was 8.1 × 10-9 M, 1.7 10-8 2.3 respectively. detected CD44v3-10 partially stained formalin-fixed paraffin-embedded tissues Collectively, useful detecting various applications.

Язык: Английский

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Clinical Impact of Exosomes in Colorectal Cancer Metastasis

ACS Applied Bio Materials, Год журнала: 2023, Номер 6(7), С. 2576 - 2590

Опубликована: Июнь 14, 2023

Cancer is a complex deadly disease that has caused global health crisis in recent epochs. Colorectal cancer (CRC) the third most common malignant gastrointestinal disease. It led to high mortality due early diagnostic failure. Extracellular vesicles (EVs) come with promising solutions for CRC. Exosomes (a subpopulation of EVs) play vital role as signaling molecules CRC tumor microenvironment. secreted from all active cells. Exosome-based molecular transport (DNA, RNA, proteins, lipids, etc.) transforms recipient cell's nature. In CRC, cell-derived exosomes (TEXs) regulate multiple events development and progression such immunogenic suppression, angiogenesis, epithelial–mesenchymal transitions (EMT), physical changes extracellular matrix (ECM), metastasis. Biofluid-circulated tumor-derived are potential tool liquid biopsy. colorectal detection creates great impact biomarker research. The exosome-associated theranostics approach state-of-the-art method. this review, we address associated progression, on screening (diagnostic prognostic biomarkers), also highlight several clinical trials, well future directions exosome-based Hopefully, it will encourage researchers develop theranostic fight

Язык: Английский

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The current landscape of spatial biomarkers for prediction of response to immune checkpoint inhibition

npj Precision Oncology, Год журнала: 2024, Номер 8(1)

Опубликована: Авг. 13, 2024

Enabling the examination of cell-cell relationships in tissue, spatially resolved omics technologies have revolutionised our perspectives on cancer biology. Clinically, development immune checkpoint inhibitors (ICI) has advanced therapeutics. However, a major challenge effective implementation is identification predictive biomarkers response. In this review we examine potential added value spatial response to ICI beyond current clinical benchmarks.

Язык: Английский

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Colorectal Cancer Liver Metastases: Genomics and Biomarkers with Focus on Local Therapies

Cancers, Год журнала: 2023, Номер 15(6), С. 1679 - 1679

Опубликована: Март 9, 2023

Molecular cancer biomarkers help personalize treatment, predict oncologic outcomes, and identify patients who can benefit from specific targeted therapies. Colorectal (CRC) is the third-most common cancer, with liver being most frequent visceral metastatic site. KRAS, NRAS, BRAF V600E Mutations, DNA Mismatch Repair Deficiency/Microsatellite Instability Status, HER2 Amplification, NTRK Fusions are NCCN approved actionable molecular for colorectal cancer. Additional also described be helpful in different image-guided hepatic directed therapies specifically CRLM. For example, tumors maintaining Ki-67 proliferation marker after thermal ablation have been particularly resilient to ablation. Ablation margin was shown an important factor predicting local recurrence, a ≥10 mm minimal required attain tumor control, especially mutant KRAS

Язык: Английский

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Development of a Novel Anti-CD44 Variant 5 Monoclonal Antibody C₄₄Mab-3 for Multiple Applications against Pancreatic Carcinomas

Опубликована: Янв. 31, 2023

Pancreatic cancer exhibits a poor prognosis due to the lack of early diagnostic biomarkers and resistance conventional chemotherapy. CD44 has been known as stem cell marker, plays tumor promotion drug in various cancers. Especially, splicing variants are overexpressed many carcinomas, play essential roles stemness, invasiveness or metastasis, treatments. Therefore, understanding each variant (CD44v) function distribution carcinomas is for establishment CD44-targeting therapy. In this study, we immunized mice with CD44v3–10-overexpressed Chinese hamster ovary-K1 (CHO) cells, established anti-CD44 monoclonal antibodies (mAbs). One clones (C44Mab-3; IgG1, kappa) recognized peptides 5-encoded region, indicating that C44Mab-3 specific mAb CD44v5. Moreover, reacted CHO/CD44v3–10 cells pancreatic lines (PK-1 PK-8) by flow cytometry. The apparent KD PK-1 was 7.1 × 10−10 M 1.9 10−9 M, respectively. could detect exogenous CD44v3–10 endogenous CD44v5 western blotting, stained formalin-fixed paraffin-embedded but not normal epithelial immunohistochemistry. These results indicate useful detecting applications, expected application diagnosis

Язык: Английский

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The Role of Immune Checkpoint Inhibitors in Metastatic Pancreatic Cancer: Current State and Outlook

Pharmaceuticals, Год журнала: 2023, Номер 16(10), С. 1411 - 1411

Опубликована: Окт. 4, 2023

Pancreatic ductal adenocarcinoma (PDAC) is one of the deadliest tumors, characterized by its aggressive tumor biology and poor prognosis. While immune checkpoint inhibitors (ICIs) play a major part in treatment algorithm various solid there still no evidence clinical benefit from ICI patients with metastatic PDAC (mPDAC). This might be due to several reasons, such as inherent low immunogenicity pancreatic cancer, dense stroma-rich microenvironment that precludes an efficient migration antitumoral effector T cells cancer cells, increased proportion immunosuppressive regulatory (Tregs), cancer-associated fibroblasts (CAFs), myeloid-derived suppressor (MDSCs), facilitating growth invasion. In this review, we provide overview current state ICIs mPDAC, report on biological rationale implement into strategy discuss preclinical studies trials field. Additionally, shed light challenges implementing potential future directions.

Язык: Английский

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Application of nanomaterials in early diagnosis of cancer

Nanotechnology Reviews, Год журнала: 2023, Номер 12(1)

Опубликована: Янв. 1, 2023

Abstract Early diagnosis of cancer is beneficial to improve the outcomes clinical treatment and reduce mortality. Nanomaterials (NMs) have exhibited significant advantages in early cancer. Due integrated structure features NMs, they can easily penetrate tissue for vivo identify cells after specific conjugation with unique markers expressed on or within based biological imaging such as Raman imaging, fluorescence near-infrared whereas NMs vitro detect signals electrochemical reactions, immune colorimetric analyzing small molecules, proteins (or enzymes), genetic materials blood, urine, saliva. novel physical properties facilitate detection low concentrations enable sensitive capture biomarkers, achieving detection. However, a comprehensive summary has not been reported. This article provides detailed review types diagnostic mechanisms diagnosis, emphasizing new perspectives use proposing development prospects NMs. will provide ideas scientific references application diagnosis.

Язык: Английский

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Development of a Novel Anti-CD44 variant 6 Monoclonal Antibody for Multiple Applications against Colorectal Carcinomas

Опубликована: Янв. 9, 2023

CD44 is a cell surface glycoprotein, and its isoforms are produced by the alternative splicing with standard variant exons. The exon containing (CD44v) overexpressed in carcinomas. CD44v6 one of CD44v, overexpression predicts poor prognosis colorectal cancer (CRC) patients. plays critical roles CRC adhesion, proliferation, stemness, invasiveness, chemoresistance. Therefore, promising target for diagnosis therapy CRC. In this study, we established anti-CD44 monoclonal antibodies (mAbs) immunizing mice CD44v3-10-overexpressed Chinese hamster ovary-K1 (CHO) cells. We then characterized them using enzyme-linked immunosorbent assay, flow cytometry, western blotting, immunohistochemistry. One clones (C44Mab-9; IgG1, kappa) reacted peptide 6-encoded region, indicating that C44Mab-9 recognizes CD44v6. Furthermore, CHO/CD44v3-10 cells or lines (COLO201 COLO205) cytometry. apparent KD CHO/CD44v3-10, COLO201, COLO205 was 8.1 × 10−9 M, 1.7 10−8 2.3 respectively. detected CD44v3-10 partially stained formalin-fixed paraffin-embedded tissues Collectively, useful detecting various applications.

Язык: Английский

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Diagnostic accuracy of tumor M2-pyruvate kinase (tM2-PK) as a non-invasive biomarker in colorectal cancer: A systematic review and meta-analysis

Clinical Biochemistry, Год журнала: 2023, Номер 120, С. 110652 - 110652

Опубликована: Сен. 25, 2023

Язык: Английский

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Survival Contradiction in Stage II, IIIA, And IIIB Colon Cancer: A Surveillance, Epidemiology, and End Result-Based Analysis

Evidence-based Complementary and Alternative Medicine, Год журнала: 2022, Номер 2022, С. 1 - 8

Опубликована: Ноя. 16, 2022

There exists an inconsistency between stage and survival in the current American Joint Committee on Cancer (AJCC) staging system for colon cancer. In this study, we compared clinicopathological characteristics prognosis of cancer patients with II, IIIA, IIIB disease based surveillance, epidemiology, end results (SEER) database. Kaplan-Meier analysis was used to generate overall (OS) cancer-specific (CSS) curves. The Cox regression employed identify risk factors. competing model completed by cumulative incidence function Gray’s test. final population 31,361 patients, curve showed that IIIA had highest OS CSS, followed IIA IIIB, IIB IIC worst CSS. model, proven be independent prognostic factor. lowest 5-year death rate stages IIIB. conclusion, worse than while lower

Язык: Английский

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