Plasma-Activated Medium Inhibited the Proliferation and Migration of Non-Small Cell Lung Cancer A549 Cells in 3D Culture DOI Open Access

Zhidan Sun,

Chenglong Ding,

Yuhan Wang

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(24), С. 13262 - 13262

Опубликована: Дек. 10, 2024

Lung cancer is the most common type of malignant tumor worldwide. Plasma-activated medium (PAM) an innovative treatment method that has received considerable scientific attention. The objective this study to evaluate effects PAM on anti-tumor characteristics non-small cell lung (NSCLC) cells in two-dimensional (2D) and three-dimensional (3D) cultures. proliferative migratory capabilities A549 2D 3D cultures were assessed using MTT, migration, invasion assays, cycle, respectively. also investigated impact changes content intracellular extracellular reactive species analyzed protein expression Western Blot method. inhibited viability, abilities both cultures, suppressed epithelial–mesenchymal transition (EMT) process, downregulated RAS/ERK signaling pathway, which effectively spheroid formation. Additionally, effect was mediated through ROS-induced oxidative reactions, exhibited greater cytotoxicity culture compared culture. As 2D, model provides a viable vitro for studying mechanisms cancer. represents effective new NSCLC.

Язык: Английский

Extracellular Matrix Components and Mechanosensing Pathways in Health and Disease DOI Creative Commons
Aikaterini Berdiaki, Monica Neagu, Petros Tzanakakis

и другие.

Biomolecules, Год журнала: 2024, Номер 14(9), С. 1186 - 1186

Опубликована: Сен. 20, 2024

Glycosaminoglycans (GAGs) and proteoglycans (PGs) are essential components of the extracellular matrix (ECM) with pivotal roles in cellular mechanosensing pathways. GAGs, such as heparan sulfate (HS) chondroitin (CS), interact various cell surface receptors, including integrins receptor tyrosine kinases, to modulate responses mechanical stimuli. PGs, comprising a core protein covalently attached GAG chains, serve dynamic regulators tissue mechanics behavior, thereby playing crucial role maintaining homeostasis. Dysregulation GAG/PG-mediated pathways is implicated numerous pathological conditions, cancer inflammation. Understanding intricate mechanisms by which GAGs PGs forces holds promise for developing novel therapeutic strategies targeting mechanotransduction disease. This comprehensive overview underscores importance key mediators homeostasis their potential targets mitigating mechano-driven pathologies, focusing on

Язык: Английский

Процитировано

12

Tumor Microenvironment: Nurturing Cancer Cells for Immunoevasion and Druggable Vulnerabilities for Cancer Immunotherapy DOI
Hongyang Yu, Jinyang Li, Shiyin Peng

и другие.

Cancer Letters, Год журнала: 2024, Номер 611, С. 217385 - 217385

Опубликована: Дек. 6, 2024

Язык: Английский

Процитировано

5

Deciphering the Interplay of the PD‐L1/MALT1/miR‐200a Axis During Lung Cancer Development DOI Open Access
Sherien M. El‐Daly,

Sahar S. Abdelrahman,

Mona A. El‐Bana

и другие.

Biotechnology and Applied Biochemistry, Год журнала: 2025, Номер unknown

Опубликована: Фев. 5, 2025

Abstract Lung cancer remains a leading cause of cancer‐related mortality worldwide. Our study investigates the involvement PD‐L1/MALAT1/miR‐200a‐3p axis in lung tumor progression using murine model carcinogenesis. tumors were induced rats, which divided into groups and sacrificed at different stages development. A histopathological examination was performed to assess progression. Immunohistochemistry applied evaluate expression Ki‐67 programmed death‐ligand 1 (PD‐L1). The level carcinoembryonic antigen (CEA) analysis metastasis‐associated adenocarcinoma transcript (MALAT1), miR‐200a‐3p, zinc finger E‐box binding homeobox (ZEB1) evaluated for each stage induction. Immunohistochemical demonstrated progressive upregulation proliferative marker immune checkpoint protein PD‐L1 during induction process, indicative enhanced proliferation evasion. Additionally, CEA levels revealed increase across stages, with significant advanced highlighting its clinical relevance as biomarker Expression dynamic MALAT1 downregulation miR‐200a induction, correlated elevated expression, suggesting that negative correlation between is involved development tumors. ZEB1 exhibited notable consistent association aggressive cancer. findings underscore interplay molecular pathways potential diagnostic therapeutic implications axis.

Язык: Английский

Процитировано

0

Advances in non-small cell lung cancer mechanomedicine: deciphering the signaling networks that govern tumor-TME interactions DOI Creative Commons
Antonios N. Gargalionis, Kostas A. Papavassiliou, Efthimia K. Basdra

и другие.

Journal of Experimental & Clinical Cancer Research, Год журнала: 2024, Номер 43(1)

Опубликована: Ноя. 30, 2024

Abstract Cells from the tumor microenvironment (TME) interact with cells in non-small cell lung cancer (NSCLC) to form a reciprocal crosstalk which influences growth, proliferation, metastasis and multidrug response. This is modulated by TME mechanical inputs, elicit processes of mechanosensing mechanotransduction. Recent advances unveiling these signaling networks establish interdisciplinary field mechanomedicine exploit emerging diagnostic, predictive therapeutic tools for more effective NSCLC treatments.

Язык: Английский

Процитировано

2

Mechanotransduction Circuits in Human Pathobiology DOI Open Access
Antonios N. Gargalionis, Kostas A. Papavassiliou, Athanasios G. Papavassiliou

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(7), С. 3816 - 3816

Опубликована: Март 29, 2024

It is widely acknowledged that mechanical forces exerted throughout the human body are critical for cellular and tissue homeostasis [...].

Язык: Английский

Процитировано

1

Harnessing Tumor Mechanobiology in NSCLC Treatment DOI
Kostas A. Papavassiliou, Vassiliki A. Gogou, Athanasios G. Papavassiliou

и другие.

Archivos de Bronconeumología, Год журнала: 2024, Номер 60(8), С. 523 - 525

Опубликована: Апрель 19, 2024

Язык: Английский

Процитировано

1

DDX18 Facilitates the Tumorigenesis of Lung Adenocarcinoma by Promoting Cell Cycle Progression through the Upregulation of CDK4 DOI Open Access

Bingbing Feng,

Xinying Wang, Ding Qiu

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(9), С. 4953 - 4953

Опубликована: Май 1, 2024

Lung adenocarcinoma (LUAD) is the most prevalent and aggressive subtype of lung cancer, exhibiting a dismal prognosis with five-year survival rate below 5%. DEAD-box RNA helicase 18 (

Язык: Английский

Процитировано

1

Exploiting tumor mechanomedicine for lung cancer treatment DOI
Antonios N. Gargalionis, Kostas A. Papavassiliou, Efthimia K. Basdra

и другие.

Cancer Letters, Год журнала: 2024, Номер 604, С. 217229 - 217229

Опубликована: Сен. 12, 2024

Язык: Английский

Процитировано

0

Targeting Nanoparticles to Lung Cancer-derived A549 Cells Based on Changes on Interstitial Stiffness in Biomimetic Models DOI Creative Commons
Afia Ibnat Kohon, Kun Man,

Ala Hessami

и другие.

iScience, Год журнала: 2024, Номер 27(10), С. 111015 - 111015

Опубликована: Сен. 23, 2024

Язык: Английский

Процитировано

0

Plasma-Activated Medium Inhibited the Proliferation and Migration of Non-Small Cell Lung Cancer A549 Cells in 3D Culture DOI Open Access

Zhidan Sun,

Chenglong Ding,

Yuhan Wang

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(24), С. 13262 - 13262

Опубликована: Дек. 10, 2024

Lung cancer is the most common type of malignant tumor worldwide. Plasma-activated medium (PAM) an innovative treatment method that has received considerable scientific attention. The objective this study to evaluate effects PAM on anti-tumor characteristics non-small cell lung (NSCLC) cells in two-dimensional (2D) and three-dimensional (3D) cultures. proliferative migratory capabilities A549 2D 3D cultures were assessed using MTT, migration, invasion assays, cycle, respectively. also investigated impact changes content intracellular extracellular reactive species analyzed protein expression Western Blot method. inhibited viability, abilities both cultures, suppressed epithelial–mesenchymal transition (EMT) process, downregulated RAS/ERK signaling pathway, which effectively spheroid formation. Additionally, effect was mediated through ROS-induced oxidative reactions, exhibited greater cytotoxicity culture compared culture. As 2D, model provides a viable vitro for studying mechanisms cancer. represents effective new NSCLC.

Язык: Английский

Процитировано

0