Deciphering the tumour microenvironment of clear cell renal cell carcinoma: Prognostic insights from programmed death genes using machine learning

Journal of Cellular and Molecular Medicine, Год журнала: 2024, Номер 28(13)

Опубликована: Июль 1, 2024

Abstract Clear cell renal carcinoma (ccRCC), a prevalent kidney cancer form characterised by its invasiveness and heterogeneity, presents challenges in late‐stage prognosis treatment outcomes. Programmed death mechanisms, crucial eliminating cells, offer substantial insights into malignant tumour diagnosis, prognosis. This study aims to provide model based on 15 types of Cell Death‐Related Genes (PCDRGs) for evaluating immune microenvironment ccRCC patients. patients from the TCGA arrayexpress cohorts were grouped PCDRGs. A combination using Lasso SuperPC was constructed identify prognostic gene features. The cohort validated model, confirming robustness. Immune analysis, facilitated PCDRGs, employed various methods, including CIBERSORT. Drug sensitivity analysis guided clinical decisions. Single‐cell data enabled scoring, subsequent pseudo‐temporal cell–cell communication analyses. PCDRGs signature established TCGA‐KIRC data. External validation underscored model's superiority over traditional Furthermore, our single‐cell unveiled roles PCDRG‐based subgroups ccRCC, both progression intercellular communication. Finally, we performed CCK‐8 assay other experiments investigate csf2 . In conclusion, these findings reveal that inhibit growth, infiltration movement cells associated with clear carcinoma. introduces benefiting while shedding light pivotal role programmed genes shaping

Язык: Английский

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The Multifaceted Roles of MicroRNA-181 in Stem Cell Differentiation and Cancer Stem Cell Plasticity

Cells, Год журнала: 2025, Номер 14(2), С. 132 - 132

Опубликована: Янв. 17, 2025

Stem cells are undifferentiated or partially differentiated with an extraordinary ability to self-renew and differentiate into various cell types during growth development. The epithelial–mesenchymal transition (EMT), a critical developmental process, enhances stem cell-like properties in cells, is associated both normal function the formation of cancer cells. Cell stemness EMT often coexist interconnected contexts. Cancer tumor population that drives tumorigenesis, progression, drug resistance, metastasis. differentiation generation regulated by numerous molecules, including microRNAs (miRNAs). These miRNAs, particularly through modulation EMT-associated factors, play major roles controlling This review presents up-to-date summary regulatory miR-181 human stemness. We outline studies from current literature summarize miR-181-controlled signaling pathways responsible for driving emergence Given its role regulating stemness, promising target influencing fate. Modulation expression has been found be altered cells’ biological behaviors significantly improve treatment outcomes. Additionally, we discuss challenges miRNA-based therapies targeted delivery nanotechnology-based systems.

Язык: Английский

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Emerging Role of Hypoxia-Inducible Factors (HIFs) in Modulating Autophagy: Perspectives on Cancer Therapy

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(4), С. 1752 - 1752

Опубликована: Фев. 19, 2025

Hypoxia-inducible factors (HIFs) are master regulators of cellular responses to low oxygen levels and modulate autophagy, a conserved process essential for maintaining homeostasis. Under hypoxic conditions, HIFs regulate the expression autophagy-related genes influence autophagic flux stress responses. Dysregulated hypoxia-induced autophagy promotes cancer cell survival, metabolism, metastasis, thereby contributing treatment resistance. Targeting HIF-mediated pathways or modulating processes offers potential improve traditional therapies overcome drug Pharmacological inhibitors either alone in combination with other treatments, may disrupt pro-survival mechanisms within tumor microenvironment. Further research is needed elucidate intricate interplay between HIF signaling machinery cells. Understanding these could pave way novel therapeutic strategies enhance outcomes combat This review highlights complex relationship development therapy, offering insights into how targeting patient outcomes.

Язык: Английский

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SATB2 promotes radiation resistance of esophageal squamous cell carcinoma by regulating epithelial-to-mesenchymal transition via the Wnt/β-catenin pathway

Frontiers in Oncology, Год журнала: 2025, Номер 15

Опубликована: Фев. 26, 2025

Radioresistance remains a predominant factor contributing to local recurrence in esophageal squamous cell carcinoma (ESCC). SATB2, as transcriptional co-gene, may affect the radioresistance of cancer cells. Consequently, this study aims elucidate mechanism by which SATB2 modulates radiotherapy resistance cancer. We identified highly expressed genes associated with ESCC using MSigDB database and conducted survival correlation analysis. A radioresistant line (KYSE150R) was established gradient dose method, RT-qPCR used detect expression KYSE150 KYSE150R CCK-8, Transwell, colony formation assay, scratching were performed determine evaluate proliferation, migration, invasion. Furthermore, levels mRNA protein correlated WB RT-qPCR. Mitochondrial membrane potential apoptosis detection kits level apoptosis. Finally, mouse subcutaneous xenograft tumor model employed role on vivo. Bioinformatics analysis indicated that is linked increased drug The results demonstrated suppression decelerates proliferation accelerates apoptosis, inhibits GSK-3β (Ser9) phosphorylation, reduces β-catenin target gene C-myc. addition Wnt/β-catenin signaling pathway agonist (CHIR-99021) reversed these effects. Xenograft studies mice revealed knockdown reduced radioresistance. concluded dysregulate pathway, thereby facilitating EMT progression conferring

Язык: Английский

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Effects of Melatonin on the Expression of Invasion‐Related Markers (MMP2 and MMP9) in Breast Cancer Cells

Journal of Cellular Biochemistry, Год журнала: 2025, Номер 126(3)

Опубликована: Март 1, 2025

ABSTRACT Breast cancer is one of the most common types in women, and metastasis a leading cause mortality patients with this disease. This study investigated effects melatonin, natural hormone, on migration cells two cell lines, MCF‐7 MDA‐MB‐231. MDA‐MB‐231 were cultured their respective media. The effective dose melatonin each line was determined using MTT assay. IC50 assessed wound‐healing expression invasion‐related genes (MMP2 MMP9), as well receptors MT1 MT2, analyzed Real‐Time RT‐PCR. assay results indicated that 48 h treatment at doses 2.5 3.5 M significantly reduced cells. In addition, decreased markers both lines. Melatonin also increased MT2 MMP2 MMP9 by ( p < 0.05). Our indicate naturally occurring compound, possesses potential to inhibit movement spread breast elevating levels receptors, resulting reduction matrix metalloproteinases 2 9 expression.

Язык: Английский

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Recent progress in exosomal non-coding RNAs research related to idiopathic pulmonary fibrosis

Frontiers in Genetics, Год журнала: 2025, Номер 16

Опубликована: Март 27, 2025

Idiopathic Pulmonary Fibrosis (IPF) is a progressive interstitial lung disease characterized by unknown etiology and limited therapeutic options. Recent studies implicate exosomal non-coding RNAs (ncRNAs) as crucial regulators in IPF. These ncRNAs, including long (lncRNAs), microRNAs (miRNAs), circular (circRNAs), are involved cellular processes through various mechanisms of selective packaging, intercellular communication, signaling pathway integration. LncRNAs such LINC00470 PVT1 exhibit pro-fibrotic effects, while others like lnc-DC THRIL show inhibitory roles; some, UCA1 MALAT1, demonstrate bidirectional regulation. In miRNAs, agents (e.g., miR-486, miR-223) contrast with miRNAs miR-34a, miR-126), miR-21 miR-155 display dual functions. Similarly, circRNAs circ_0000479 circ_0026344 promote fibrosis, whereas circ_0000072 circ_0000410 act inhibitors, certain circ_002178 circ_0001246) exhibiting complex regulatory effects. Exosomal ncRNAs modulate key pathways, TGF-β Wnt/β-catenin, influencing IPF progression. Despite their potential, challenges remain exosome isolation, functional characterization clinical translation. Addressing these barriers innovative research strategies essential to leverage the management treatment This review comprehensively examines roles IPF, elucidates interactions, discusses future perspectives enhance understanding for this disease.

Язык: Английский

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The influence of exogenous melatonin on the morphological features of transplanted melanoma B16.

Morphology, Год журнала: 2025, Номер unknown

Опубликована: Март 31, 2025

Suppression of the function pineal gland by constant lighting leads to depletion melatonin-producing this gland, and an increase in incidence age-related pathologies, including neoplasms. Melatonin has important antitumor effect, among which antiproliferative effect stands out. Fge composition melanoma patients shifted towards proportion older age groups. There is evidence that melatonin deficiency one factors causing development Study on morphological features transplantable B16 mice. The study was conducted male BDF1 hybrid mice (n=60) aged 8 weeks, weighing 21–22 g, divided into two equal Animals both groups underwent transplantation B16/F10 melanoma. From day 1 study, animals experimental group received drug (Sigma, USA) at a dosage 5 mg/kg body weight. On 15th after tumor, were removed from experiment. lungs liver group. A pathomorphological examination tumor carried out, presence metastases determined. It been established use reduces mortality animals, frequency metastasis, decrease its size; animal tumors there are signs regression form foci dystrophic alterative changes. showed exogenous pronounced against results obtained require more thorough multidisciplinary understand mechanisms for achieving but suggest prospects therapy malignant diseases.

Язык: Английский

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Epithelial–Mesenchymal Transitions Leading to Conceptus Adhesion in Ruminants: Early Pregnancy Events in Cattle

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(8), С. 3772 - 3772

Опубликована: Апрель 16, 2025

Trophoblast–endometrium interactions play a critical role in the processes of conceptus elongation, attachment, and adhesion, followed by placental development during early pregnancy ruminants. The attachment between uterine epithelium trophoblast cells, which is epithelial nature, requires to mesenchymal transition (EMT), where fetal trophoblasts come into contact with maternal cells without fully invading tissues. Understanding developmental period driving EMT utero ruminants fundamental improving fertility through prevention failure enhancing overall reproductive efficiency livestock. This review highlights key events necessary for progress properly towards firm adhesion endometrium, focusing on trophoblast–endometrium interactions. field holds potential elucidate molecular cellular mechanisms associated endometrium leading reduced embryonic losses enhanced economic sustainability developing effective management strategies.

Язык: Английский

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LINC00513 promotes colorectal cancer malignant progression by binding with IGF2BP1 to enhance the stability of connective tissue growth factor mRNA

Epigenomics, Год журнала: 2024, Номер 16(14), С. 985 - 998

Опубликована: Июль 17, 2024

Aim: This study aimed to investigate the role of LINC00513 in colorectal cancer (CRC) progression. Materials & methods: Cell proliferation was evaluated using Counting Kit-8. migration detected with transwell assay. RNA pull-down applied for verifying interactions between LINC00513, IGF2BP1 and connective tissue growth factor (CTGF). Results: CTGF levels were upregulated CRC. Knockdown significantly inhibited malignant behavior CRC cells. increased mRNA stability by binding IGF2BP1. Furthermore, overexpression or reversed inhibitory effect shRNA on Conclusion: promoted cell behaviors through IGF2BP1/CTGF.

Язык: Английский

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Investigating the role of HSP90 in cancer cell phenotypic plasticity

Journal of Breast Cancer Research, Год журнала: 2024, Номер 4(1), С. 5 - 10

Опубликована: Апрель 29, 2024

The emerging importance of epigenetic gene regulation in cancer progression necessitates not only our understanding which genes are potential targets but also what mechanisms employed targeting those genes. Understanding the that promote evolution normal genome and epigenome is central to how cells adapt chemotherapy. Our previous investigations have shown heat shock protein 90 (HSP90) has a critical role through histone acetylation phenotypic plasticity. We recently extended these results an A549 lung model test HSP90 plasticity regarding multi-drug resistance epithelial-to-mesenchymal transition phenotypes. over-expressed multiple cancers with poor prognosis. propose inhibition lower making them more susceptible chemotherapeutic intervention. Here we review context broader field

Язык: Английский

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