Comprehensive Analysis of the Proteome of S. cerevisiae Wild-Type and pdr5Δ Cells in Response to Bisphenol A (BPA) Exposure

Microorganisms, Год журнала: 2025, Номер 13(1), С. 114 - 114

Опубликована: Янв. 8, 2025

Bisphenol A, an endocrine-disrupting compound, is widely used in the industrial production of plastic products. Despite increasing concerns about its harmful effects on human health, animals, and environment, use BPA has been banned only infant products, cellular processes are not fully understood. To investigate impact eukaryotic cells, we analyzed proteome changes wild-type PDR5-deleted S. cerevisiae strains exposed to different doses using sample multiplexing-based proteomics. We found that ABC multidrug transporter Pdr5 plays important role protecting yeast cells from toxicity, with absence significantly sensitizing BPA. inhibited growth a dose-dependent manner, more pronounced effect cells. Proteomic analysis revealed induces widespread protein abundance, including upregulation metabolic pathways such as arginine biosynthesis downregulation mitochondrial proteins. Additionally, observed markers stress induced by identifying multiple stress-induced proteins were upregulated this compound. As affected have shown be evolutionarily conserved, these insights can advance our understanding BPA's broader health.

Язык: Английский

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Tracing the Footprint of Microplastics: Transport Mechanism, Degradation, and Remediation in Marine Environment

Water Air & Soil Pollution, Год журнала: 2025, Номер 236(2)

Опубликована: Янв. 24, 2025

Язык: Английский

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Microplastic Pollution in Soil and Water and the Potential Effects on Human Health: A Review

Processes, Год журнала: 2025, Номер 13(2), С. 502 - 502

Опубликована: Фев. 11, 2025

The presence of microplastics in the environment has increased due to anthropogenic activities; it is estimated that 15 million kilograms plastic waste accumulate ocean annually. Pollution permeates every inch from food chain water bottles floating on surface. This monolith pollution made up all kinds marine debris and contains 1.8 trillion pieces plastic, covering an area twice size Texas. objective this review show advances study emerging problems, specifically soil their potential effects health. In addition, have synergy with residual contaminants exist such as textile waste, organic matter, pathogens, etc. causes damage aquatic organisms makes nutrient transfer more complex many these species. There a report estimates liabilities related will cost industry 100,000 dollars lawsuits for damages losses, which 20,000 occur United States. can generate indicators current effect public policies try control growth pollutant environment. It important discuss routes generation microplastics, distribution, cosmetics involved fast fashion glitter evaluate physical, chemical, biological, toxicological environment, proposing path future be followed regarding research topic.

Язык: Английский

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Abundance, characteristics, and potential human intake of microplastic contamination in tap water: A study of water supply treatment plants in Rayong Province, Thailand

Journal of Water Process Engineering, Год журнала: 2025, Номер 72, С. 107460 - 107460

Опубликована: Март 17, 2025

Язык: Английский

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Interaction of Polystyrene Nanoplastics with Biomolecules and Environmental Pollutants: Effects on Human Hepatocytes

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(7), С. 2899 - 2899

Опубликована: Март 22, 2025

The inevitable exposure of humans to micro/nanoplastics has become a pressing global environmental issue, with growing concerns regarding their impact on health. While the direct effects human health remain largely unknown, increasing attention is being given potential role as carriers pollutants and organic substances. This study investigates toxicity 500 nm polystyrene nanoplastics (NPs) hepatocytes (HepG2) in vitro, both alone combination cadmium (Cd), hazardous heavy metal prevalent pollutant. One-hour 100 µg/mL NPs causes significant increase ROS production (+25% compared control) but cell viability remains unaffected even at concentrations much higher than levels. Interestingly, significantly reduce Cd cytotoxicity LC50 (cell control: 55.4% for 50 µM Cd, 66.9% + 10 NPs, 68.4% NPs). Additionally, do not alter cellular lipid content after short-term (24 h). However, when fatty acids are added medium, appear sequester acids, reducing availability impairing uptake by cells dose-dependent manner. We confirmed Dynamic Light Scattering Scanning Electron Microscopy interaction between free acids. Although exhibited minimal our experimental model, collectively findings suggest that predicting extremely challenging, due specific components biological matrix.

Язык: Английский

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Microplastics as benzo-a-pyrene carriers: genotoxicity assessment simulating human gastric digestion

Archives of Toxicology, Год журнала: 2025, Номер unknown

Опубликована: Апрель 19, 2025

Abstract Microplastic particles (MPs) are ubiquitous environmental pollutants that can remain in ecosystems for prolonged periods. Plastic materials undergo various degradation processes driven by chemical, physical, and biological factors alter their size, shape, composition, bioavailability. The gastrointestinal tract is the primary pathway through which MPs absorbed, raising concerns as they transport harmful microorganisms into body. Despite widespread presence, effects of exposure to vehicle toxins still not well understood. In this study, we rigorously simulated photoaging polystyrene two distinct sizes (1 µm 5 µm) confirmed capacity adsorb benzo[a]pyrene, a known carcinogen. Moreover, explored capabilities these analyzed genotoxic on liver cells under gastric digestion conditions. Our findings reveal enriched with BaP release toxic compound when ingested exposed juices, markedly increasing toxicity compared individual components. This research underscores alarming potential exacerbate risks associated human health.

Язык: Английский

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Microplastics in livestock manure and compost: environmental distribution, degradation behavior, and their impact on antibiotic resistance gene dissemination

Chemical Engineering Journal, Год журнала: 2025, Номер unknown, С. 162881 - 162881

Опубликована: Апрель 1, 2025

Язык: Английский

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