bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Окт. 24, 2023
Glycans
play
critical
roles
in
cellular
signaling
and
function.
Unlike
proteins,
glycan
structures
are
not
templated
from
genes
but
the
concerted
activity
of
many
genes,
making
them
historically
challenging
to
study.
Here,
we
present
a
strategy
that
utilizes
pooled
CRISPR
screens
lectin
microarrays
uncover
characterize
regulators
cell
surface
glycosylation.
We
applied
this
approach
study
regulation
high
mannose
glycans
-
starting
structure
all
asparagine(N)-linked-glycans.
used
expanded
network
controlling
levels,
followed
by
fully
measure
complex
effect
select
on
glycosylation
globally.
Through
this,
elucidated
how
two
novel
TM9SF3
CCC
control
N-glycosylation
via
regulating
Golgi
morphology
Notably,
method
allowed
us
interrogate
function
in-depth
reveal
similar
disruption
can
lead
drastically
different
outcomes.
Collectively,
work
demonstrates
generalizable
for
systematically
dissecting
regulatory
underlying
Frontiers in Cell and Developmental Biology,
Год журнала:
2025,
Номер
12
Опубликована: Янв. 7, 2025
The
GC
(Golgi
complex)
plays
a
pivotal
role
in
the
trafficking
and
sorting
of
proteins
lipids
until
they
reach
their
final
destination.
Additionally,
acts
as
signalling
hub
to
regulate
multitude
cellular
processes,
including
cell
polarity,
motility,
apoptosis,
DNA
repair
division.
In
light
these
crucial
roles,
has
garnered
increasing
attention,
particularly
given
evidence
that
dysregulation
GC-regulated
pathways
may
contribute
onset
various
pathological
conditions.
This
review
examines
functions
GC-localised
regulating
cycle
progression,
both
mitosis
meiosis.
It
reviews
involvement
GC-resident
formation
orientation
spindle
during
roles
played
by
controlling
division,
this
also
addresses
cancer
development.
Furthermore,
TCGA
(The
Cancer
Genome
Atlas)
database
been
queried
order
retrieve
information
on
genetic
alterations
correlation
between
expression
survival
patients.
data
presented
highlight
relevance
differentiation
tumourigenesis.
ACS Nano,
Год журнала:
2023,
Номер
17(24), С. 24972 - 24987
Опубликована: Дек. 13, 2023
Tumor
metastasis
is
an
intricate
multistep
process
regulated
via
various
proteins
and
enzymes
modified
secreted
by
swollen
Golgi
apparatus
in
tumor
cells.
Thus,
complex
considered
as
important
target
for
the
remedy
of
metastasis.
Currently,
targeting
technologies
are
mostly
employed
Golgi-specific
fluorescent
probes
diagnosis,
but
their
applications
therapy
rarely
reported.
Herein,
we
proposed
a
prodrug
(INR)
that
can
destroy
apparatus,
which
consisted
indomethacin
(IMC)
moiety
retinoic
acid
(RA),
disrupting
agent.
The
linker
between
IMC
RA
was
designed
hypoxia-responsive
nitroaromatic
structure,
ensured
release
prototype
drugs
hypoxic
microenvironment.
Furthermore,
INR
could
be
assembled
with
pirarubicin
(THP),
anthracycline,
to
form
carrier-free
nanoparticle
(NP)
emulsion-solvent
evaporation
method.
A
small
amount
mPEG2000-DSPE
added
shield
positive
charges
improve
stability
obtain
PEG-modified
(PNP).
It
proved
released
cells
hypoxia
promoted
release.
destructive
effect
amplified
owing
ability
IMC,
also
inhibited
protumor
COX-2/PGE2
signaling.
Finally,
PNP
exhibited
excellent
curative
efficacy
on
4T1
primary
its
pulmonary
hepatic
molecular
therapeutic
adapted
multifarious
drug
delivery
systems
disease
models,
expanded
application
tactics
treatment.
ACS Applied Bio Materials,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 21, 2025
Golgi
apparatus
(GA)
and
endoplasmic
reticulum
(ER)
are
two
of
the
interesting
subcellular
organelles
that
critical
for
protein
synthesis,
folding,
processing,
post-translational
modifications,
secretion.
Consequently,
dysregulation
in
GA
ER
cross-talk
between
them
implicated
numerous
diseases
including
cancer.
As
a
result,
simultaneous
visualization
cancer
cells
is
extremely
crucial
developing
therapeutics.
To
address
this,
herein,
we
have
designed
synthesized
1,8-napthalimide-based
small
molecule
(AIE-GA-ER)
consisting
phenylsulfonamide
as
Golgi-ER
homing
triphenylamine-napthalimide
aggregation-induced
emission
(AIE)
triggering
moieties.
AIE-GA-ER
exhibited
remarkable
"on-off-on"
AIE
properties
THF/water
binary
solvent
system
due
to
aggregated
"on-state"
pure
THF
80%
water
THF.
Molecular
dynamic
simulations
density
functional
theory
(DFT)
calculations
underlying
mechanism
emissive
property
be
interplay
intramolecular
charge
transfer
(ICT)
stabilization
aggregation
THF,
DMSO,
water.
efficiently
homed
into
HCT-116
colon
within
15-30
min
well
noncancerous
human
retinal
epithelial
pigment
(RPE-1)
3
h
with
minimum
toxicity.
This
AIEgen
has
potential
illuminate
simultaneously
understand
chemical
biology
their
next-generation
Pharmaceutics,
Год журнала:
2025,
Номер
17(2), С. 198 - 198
Опубликована: Фев. 5, 2025
Tumor
metastasis,
the
spread
of
cancer
cells
from
primary
site
to
distant
organs,
remains
a
formidable
challenge
in
oncology.
Central
this
process
is
involvement
subcellular
organelles,
which
undergo
significant
functional
and
structural
changes
during
metastasis.
Targeting
these
specific
organelles
offers
promising
avenue
for
enhanced
drug
delivery
metastasis
therapeutic
efficacy.
This
precision
increases
potency
reduces
potential
off-target
effects.
Moreover,
by
understanding
role
each
organelle
treatments
can
be
designed
disrupt
metastatic
at
multiple
stages,
cell
migration
establishment
secondary
tumors.
review
delves
deeply
into
tumor
processes
their
connection
with
organelles.
In
order
target
biomembranes,
cell-penetrating
peptides,
localization
signal
aptamers,
small
molecules,
various
other
strategies
have
been
developed.
review,
we
will
elucidate
targeting
look
forward
prospects
domain.
International Journal of Veterinary Science and Medicine,
Год журнала:
2025,
Номер
13(1), С. 1 - 12
Опубликована: Апрель 24, 2025
Canine
hepatoid
gland
neoplasms
(HGNs)
are
significant
clinical
concerns
due
to
their
high
prevalence
and
diverse
biological
behaviour.
Human
epidermal
growth
factor
receptor
2
(HER2),
a
tyrosine
kinase
implicated
in
various
aspects
of
tumorigenesis,
has
been
extensively
studied
human
animal
but
remains
unexplored
HGNs.
This
study
aimed
assess
HER2
immunoexpression
canine
HGNs
its
association
with
clinicopathological
morphological
features.
A
total
61
formalin-fixed
paraffin-embedded
samples,
including
normal
glands
(n
=
10),
adenomas
(HGAs,
n
20),
epitheliomas
(HGEs,
16),
carcinomas
(HGCs,
15),
were
analysed
using
immunohistochemistry.
expression
was
scored
based
on
percentage
positivity
staining
intensity.
HER2-positive
detected
50%
HGEs
(score
+
)
73.3%
HGCs,
36.4%
cases
scoring
3
.
In
contrast,
all
HGAs
tissues
HER2-immunonegative.
Statistical
analysis
revealed
differences
among
neoplastic
(p
<
0.001).
Only
significantly
associated
tissue
invasion
0.007),
mitotic
count
0.033),
nuclear
pleomorphism
0.007).
These
findings
suggest
that
may
play
role
the
progression
malignant
HGNs,
particularly
HGCs.
preliminary
highlights
potential
as
diagnostic
marker
emphasizes
need
for
further
investigation
into
prognostic
value
HER2-targeted
therapy
Journal of Proteome Research,
Год журнала:
2025,
Номер
unknown
Опубликована: Май 14, 2025
Cancer
is
a
leading
cause
of
death
worldwide,
resulting
in
substantial
economic
costs.
Because
cancer
complex,
heterogeneous
group
diseases
affecting
variety
cells,
its
detection
may
sometimes
be
difficult.
Herein
we
review
large
the
gastrointestinal
cancers
(oral,
esophageal,
stomach,
pancreatic,
liver,
and
bowel
cancers)
possibility
using
glycans
conjugated
to
protein
backbones
for
less-invasive
diagnoses
than
commonly
used
endoscopic
approaches.
The
reality
bacterial
N-glycosylation
effect
epithelial
mucosa
on
gut
microbiota
are
discussed.
Current
advantages,
barriers,
advantages
prospective
use
selected
glycomic
approaches
clinical
practice
also
detailed.
