Robusta Coffee Husk Extract Increases the Number of Fibroblast and Collagen Density in Gingival Rat Periodontitis

Scientifica, Год журнала: 2025, Номер 2025(1)

Опубликована: Янв. 1, 2025

Introduction: Periodontitis is an infectious disease of periodontal tissue caused by the bacteria Porphyromonas gingivalis which can trigger formation free radicals. be treated using metronidazole but long‐term use cause negative effects. Natural ingredients such as robusta coffee husk used alternative treatment for periodontitis because its anti‐inflammatory, antioxidant, and antibacterial benefits. Aim: To determine effect extract on number fibroblasts collagen density in gingival Wistar rats induced P. . Methods: Robusta was extracted maceration process with 96% ethanol. Thirty male divided into 6 groups: healthy rat, control ( + Aquades sterile), positive metronidazole), 3 groups concentration 125, 250, 500 mg/kgBW). injection carried out buccal area left mandibular first molar mice every days. were given sondase once a day 21 The specimens then processed histologically. Hematoxylin Eosin (H&E) staining performed to observe fibroblast, observed Thricome Mallory. SPSS application examine data calculation included Shapiro–Wilk, Levene, One‐way ANOVA, post hoc LSD tests. Result: at doses mg/kgBW showed increased gingivalis. Conclusion: most effective dose 250 mg/kgBW, average value fibroblast field view 37 cells, 163 pixels.

Язык: Английский

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In Vitro Preventive Effect and Mechanism of Action of Weissella cibaria CMU against Streptococcus mutans Biofilm Formation and Periodontal Pathogens

Microorganisms, Год журнала: 2023, Номер 11(4), С. 962 - 962

Опубликована: Апрель 7, 2023

In this study, we evaluated the in vitro anti-biofilm, antibacterial, and anti-inflammatory activity of Weissella cibaria CMU (CMU), an oral probiotic, against periodontopathogens. Compared to other probiotics, showed a superior inhibitory effect on biofilm formation growth Streptococcus mutans orthodontic wires artificial teeth (p < 0.05). exerted potent antibacterial effects S. Porphyromonas gingivalis according line test. human gingival fibroblasts (HGFs) stimulated by P. gingivalis, Fusobacterium nucleatum, or Prevotella intermedia, suppressed gene expression pro-inflammatory cytokines [interleukin (IL)-6, IL-1β, IL-8, tumor necrosis factor-α] dose-dependent manner restored production tissue inhibitor metalloproteinase-1 following its inhibition it matrix metalloproteinase (MMP)-1 -3 induced periodontopathogens Moreover, needed direct contact with HGFs exert their function, indicating that they act directly cells modulate local inflammation. Our preclinical study provides evidence for potential benefits topical treatments preventing development caries periodontitis caused dysbiosis dental plaque microbiome.

Язык: Английский

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Mouse gingival single-cell transcriptomic atlas identified a novel fibroblast subpopulation activated to guide oral barrier immunity in periodontitis

eLife, Год журнала: 2023, Номер 12

Опубликована: Ноя. 28, 2023

Periodontitis, one of the most common non-communicable diseases, is characterized by chronic oral inflammation and uncontrolled tooth supporting alveolar bone resorption. Its underlying mechanism to initiate aberrant barrier immunity has yet be delineated. Here, we report a unique fibroblast subpopulation ctivated g uide (AG fibroblasts) identified in single-cell RNA sequencing gingival cell atlas constructed from mouse periodontitis models. AG fibroblasts localized beneath epithelium cervical periodontal ligament responded ligature placement discrete topical application Toll-like receptor stimulants maxillary tissue. The upregulated chemokines ligands linked putative receptors neutrophils early stages periodontitis. In established inflammation, neutrophils, together with fibroblasts, appeared induce type 3 innate lymphoid cells (ILC3s) that were primary source interleukin-17 cytokines. comparative analysis Rag2 -/- Il2rg-/- mice suggested ILC3 contributed resorption interfacing inflammation. We propose fibroblast–neutrophil–ILC3 axis as previously unrecognized which could involved complex interplay between immune contributing pathological

Язык: Английский

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Changes in cuproptosis-related gene expression in periodontitis: An integrated bioinformatic analysis

Life Sciences, Год журнала: 2024, Номер 338, С. 122388 - 122388

Опубликована: Янв. 3, 2024

Periodontitis causes inflammatory destruction of tooth-supporting tissues; however, the complex mechanism underlying its etiology remains unclear. Cuproptosis is a type cell death caused by an imbalance in intracellular copper homeostasis that leads to excess copper. However, changes expression and biological function cuproptosis-related genes (CRGs) periodontitis are not yet fully understood. This study investigated comprehensive effects differentially expressed CRGs (DE-CRGs) on via bioinformatic analysis. Nine DE-CRGs were discovered using normal gingival samples, single-cell RNA sequencing data analyzed identify them diverse clusters. We then detected correlation between immune infiltration, factors, mitochondrial dysfunction, diagnostic efficacy, predicted drugs. Moreover, DE-CRG whole tissue human fibroblast line (HGF-1) confirmed content HGF-1 cells investigated. Most trends reversed periodontal tissues clusters, which may be related proportion clusters periodontitis. Furthermore, most inconsistent with cuproptosis. In treated Porphyromonas gingivalis lipopolysaccharide (Pg-LPS), increased more than threefold, indicating although some had copper, amount have been sufficient trigger Additionally, closely associated multiple functions, antibiotic drugs, natural herbal medicines. Our findings provide overview pathogenesis treatment

Язык: Английский

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Enhancement of Fluoride’s Antibacterial and Antibiofilm Effects against Oral Staphylococcus aureus by the Urea Derivative BPU

Antibiotics, Год журнала: 2024, Номер 13(10), С. 930 - 930

Опубликована: Сен. 30, 2024

Background: The oral cavity is an important but often overlooked reservoir for Staphylococcus aureus. effective control and prevention of S. aureus colonization infection in the maxillofacial regions are crucial public health. Fluoride widely used dental care its remineralization antibacterial properties. However, effectiveness against has not been thoroughly investigated. Objectives: This study aimed to evaluate potential combining sodium fluoride (NaF) with compounds enhance antibiofilm effects Method: We found that a urea derivative significantly enhances efficacy by promoting retention ions within cells. synergistic BPU NaF were confirmed through various assays, including checkerboard time-kill growth curve analysis. These findings further supported additional methods, transmission electron microscopy (TEM), silico simulations, gene overexpression studies. Results: suggest targeting ion membrane exporters could efficacy. When combined fluoride, 1,3-Bis [3,5-bis(trifluoromethyl)phenyl]urea (BPU) showed increased inhibiting reducing established biofilms. Conclusions: novel combination represents promising therapeutic strategy treating biofilm-associated infections, offering new healthcare. To fully clinical this therapy, vivo studies essential.

Язык: Английский

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Avenanthramide-C Shows Potential to Alleviate Gingival Inflammation and Alveolar Bone Loss in Experimental Periodontitis

Molecules and Cells, Год журнала: 2023, Номер 46(10), С. 627 - 636

Опубликована: Авг. 29, 2023

Periodontal disease is a chronic inflammatory that leads to the gradual destruction of supporting structures teeth including gums, periodontal ligaments, alveolar bone, and root cementum.Recently, interests in alleviating symptoms periodontitis (PD) using natural compounds increasing.Avenanthramide-C (Avn-C) polyphenol found only oats.It known exhibit various biological properties.To date, effect Avn-C on PD pathogenesis has not been confirmed.Therefore, this study aimed verify protective effects inflammation subsequent bone erosion vitro vivo.Upregulated expression catabolic factors, such as matrix metalloproteinase 1 (MMP1), MMP3, interleukin (IL)-6, IL-8, COX2 induced by lipopolysaccharide proinflammatory cytokines, IL-1β, tumor necrosis factor α (TNF-α), was dramatically decreased treatment human gingival fibroblasts ligament cells.Moreover, ligature-induced mouse model ameliorated intra-gingival injection Avn-C.Molecular mechanism studies revealed inhibitory upregulation factors were mediated via ERK (extracellular signal-regulated kinase) NF-κB pathway activated IL-1β or p38 MAPK JNK signaling TNF-α, respectively.Based study, we recommend may be new compound can applied treatment.

Язык: Английский

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Epstein-Barr Virus Promotes Inflammatory Cytokine Production in Human Gingival Fibroblasts

International Dental Journal, Год журнала: 2024, Номер 74(3), С. 607 - 615

Опубликована: Янв. 15, 2024

Periodontitis is one of the most common chronic oral inflammatory diseases. Over past decade, herpes viruses, particularly Epstein-Barr virus (EBV), have been considered promising pathogenic candidates for periodontitis. However, specific mechanism by which EBV contributes to development periodontitis still unknown. This study aimed explore underlying response in human gingival fibroblasts (HGFs).

Язык: Английский

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Pyroptosis: A major trigger of excessive immune response in the gingiva

Oral Diseases, Год журнала: 2024, Номер 30(7), С. 4152 - 4160

Опубликована: Июнь 9, 2024

The gingival mucosal barrier, an important oral cavity plays a significant role in preventing pathogenic microorganism invasion and maintaining periodontal tissue health. Pathogenic of the mucosa produces large number cytokines. Among them, pyroptosis is player exacerbating immune-inflammatory responses, leading to destruction. However, mechanism immune response it triggers have not been fully elucidated. We provide overview recent advances understanding physical barrier inflammation-induced hyperimmunity.

Язык: Английский

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Mouse gingival single-cell transcriptomic atlas identified a novel fibroblast subpopulation activated to guide oral barrier immunity in periodontitis

eLife, Год журнала: 2023, Номер 12

Опубликована: Июнь 2, 2023

Periodontitis, one of the most common non-communicable diseases, is characterized by chronic oral inflammation and uncontrolled tooth supporting alveolar bone resorption. Its underlying mechanism to initiate aberrant barrier immunity has yet be delineated. Here, we report a unique fibroblast subpopulation ctivated g uide (AG fibroblasts) identified in single-cell RNA sequencing gingival cell atlas constructed from mouse periodontitis models. AG fibroblasts localized beneath epithelium cervical periodontal ligament responded ligature placement discrete topical application Toll-like receptor stimulants maxillary tissue. The upregulated chemokines ligands linked putative receptors neutrophils early stages periodontitis. In established inflammation, neutrophils, together with fibroblasts, appeared induce type 3 innate lymphoid cells (ILC3s) that were primary source interleukin-17 cytokines. comparative analysis Rag2 -/- Il2rg-/- mice suggested ILC3 contributed resorption interfacing inflammation. We propose fibroblast–neutrophil–ILC3 axis as previously unrecognized which could involved complex interplay between immune contributing pathological

Язык: Английский

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Increasing fibroblasts and gingival collagen density in periodontitis rats by using cassava leaf extract

Journal of Taibah University Medical Sciences, Год журнала: 2023, Номер 18(6), С. 1321 - 1328

Опубликована: Май 19, 2023

Porphyromonas gingivalis, as the main etiology of periodontitis, causes inflammation in periodontal tissue, which triggers immune response, fibroblast decline, and collagen destruction, generating attachment loss. Fibroblasts perform a fundamental role repair process tissue. This study examined potential cassava leaf extract increasing quantity fibroblasts density gingiva rats with periodontitis. A posttest-only control group was used this study. The experiment involved 24 male Wistar divided into four different groups: group, induced by P. gingivalis given aquadest, metronidazole, extract. Gingival tissue taken after euthanasia, histological preparations were made, observed. One-way analysis variance revealed that showed notable difference between each (p < 0.05), interestingly, there no significant metronidazole least test results > 0.05). Cassava has to increase periodontitis rat models.

Язык: Английский

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