Effect of resistance training plus enriched probiotic supplement on sestrin2, oxidative stress, and mitophagy markers in elderly male Wistar rats

Scientific Reports, Год журнала: 2024, Номер 14(1)

Опубликована: Апрель 2, 2024

Abstract This study aimed to determine the effects of resistance training combined with a probiotic supplement enriched vitamin D and leucine on sestrin2, oxidative stress, antioxidant defense, mitophagy markers in aged Wistar rats. Thirty-five male rats were randomly assigned two age groups (old 18–24 months young 8–12 weeks age) then divided into five groups, including (1) old control (OC: n = 5 + 2 for reserve all groups), (2) (YC: 5), (3) (OR: (4) plus (ORS: group (OS: 5). Training performed ladder climbing 3 times per week 8 weeks. intensity was inserted progressively, values equal 65, 75, 85, determining rats' maximal carrying load capacity. Each animal made climbs each session, time climb between 12 15 s, although not subject evaluation, pattern different animals. Old received 1 ml by oral gavage addition standard feeding, h post sessions. Forty-eight hours after end program, blood samples taken, sacrificed achieve muscle samples. After training, total capacity superoxide dismutase activity levels increased both interventions. A synergistic effect observed capacity, dismutase, PTEN-induced kinase 1. Sestrin decreased intervention groups. These results suggest that can boost defense while potentially decreasing strength loss.

Язык: Английский

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A Computational Approach for the Discovery of Novel DNA Methyltransferase Inhibitors

Current Issues in Molecular Biology, Год журнала: 2024, Номер 46(4), С. 3394 - 3407

Опубликована: Апрель 16, 2024

Nowadays, the explosion of knowledge in field epigenetics has revealed new pathways toward treatment multifactorial diseases, rendering key players epigenetic machinery focus today’s pharmaceutical landscape. Among enzymes, DNA methyltransferases (DNMTs) are first studied as inhibition targets for cancer treatment. The increasing clinical interest DNMTs led to advanced experimental and computational strategies search novel DNMT inhibitors. Considering importance a promising trend, present study attempted discover inhibitors natural origin against using combination structure ligand-based approaches. Particularly, pharmacophore-based virtual screening was performed, followed by molecular docking dynamics simulations order establish an accurate robust selection methodology. Our protocol prioritized five natural-derived compounds, derivatives coumarins, flavones, chalcones, benzoic acids, phenazine, bearing completely diverse chemical scaffolds from FDA-approved “Epi-drugs”. Their total inhibitory activity evaluated, revealing results derived hits with ranging within 30–45% at 100 µM tested compounds.

Язык: Английский

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3D-bioprinted anti-senescence organoid scaffolds repair cartilage defect and prevent joint degeneration via miR-23b/ELOVL5-mediated metabolic rewiring

Chemical Engineering Journal, Год журнала: 2024, Номер 491, С. 152049 - 152049

Опубликована: Май 8, 2024

Язык: Английский

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Underlying Mechanisms of the Protective Effects of Lifestyle Factors in the Prevention of Age-Related Diseases

Archives of Medical Research, Год журнала: 2024, Номер 55(5), С. 103014 - 103014

Опубликована: Июнь 12, 2024

Язык: Английский

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Personalized epigenetics of aging

Elsevier eBooks, Год журнала: 2024, Номер unknown, С. 657 - 691

Опубликована: Янв. 1, 2024

Язык: Английский

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