Glioma Stem Cells: GPRC5A as a Novel Predictive Biomarker and Therapeutic Target Associated with Mesenchymal and Stemness Features DOI Creative Commons
Sara Sadat Aghamiri, Rada Amin

Applied Sciences, Год журнала: 2024, Номер 14(18), С. 8482 - 8482

Опубликована: Сен. 20, 2024

Glioblastoma multiforme (GBM) represents the deadliest form of brain cancer, characterized by complex interactions within its microenvironment. Despite understanding GBM biology, remains highly resistant to any therapy. Therefore, defining innovative biomarkers in can provide insights into tumor biology and potential therapeutic targets. In this study, we explored GPRC5A serve as a pertinent biomarker for GBM. We utilized GBM-TCGA dataset presented reproducible bioinformatics analysis our results. identified that expression was significantly upregulated compared normal tissues, with higher levels correlating poor overall survival (OS) progression-free interval (PFI). Moreover, it associated key genetic mutations, particularly NF1 PTEN strongly correlated mesenchymal stem-like phenotype. also predominantly aggressive features, including hypoxia, high extracellular matrix (ECM) environments, extensive stromal immune infiltrations. Its strong correlation markers hypoxic regions underscores target These findings valuable role pathology impact stratifications treatment strategies.

Язык: Английский

Exosomes as drug delivery systems in glioma immunotherapy DOI Creative Commons
Xinqing Hao, Shiming Wang, Liang Wang

и другие.

Journal of Nanobiotechnology, Год журнала: 2024, Номер 22(1)

Опубликована: Июнь 18, 2024

Abstract Recently, the significant benefits of cancer immunotherapy for most cancers have been demonstrated in clinical and preclinical studies. However, efficacy these immunotherapies gliomas is limited, owing to restricted drug delivery insufficient immune activation. As carriers, exosomes offer advantages low toxicity, good biocompatibility, intrinsic cell targeting, which could enhance glioma efficacy. a review exosome-based systems has not presented. This introduces current problems role addressing issues. Meanwhile, preparation application strategies are discussed, especially enhancing immunogenicity reversing immunosuppressive tumor microenvironment. Finally, we briefly describe challenges translation. We anticipate that this will guide use as carriers immunotherapy. Graphical

Язык: Английский

Процитировано

5

Photodynamic and Sonodynamic Therapy Synergy: Mechanistic Insights and Cellular Responses Against Glioblastoma Multiforme DOI
Swati Sharma,

Geetanjali B. Gone,

Parikshit Roychowdhury

и другие.

Journal of drug targeting, Год журнала: 2024, Номер unknown, С. 1 - 23

Опубликована: Ноя. 18, 2024

Glioblastoma multiforme (GBM), the most aggressive form of brain cancer, poses substantial challenges to effective treatment due its complex and infiltrative nature, making it difficult manage. Photodynamic therapy (PDT) sonodynamic (SDT), have emerged as promising individual options against GBM their least-invasive approach. However, both PDT SDT drawbacks that require careful consideration. A combination using light sound waves has gained attention, offering new avenues overcome from therapies. Sono-photodynamic (SPDT) been used various tumors. Researchers are considering SPDT a favorable alternative conventional therapies for GBM. offers complementary mechanisms action, including production ROS, disruption cellular structures, induction apoptosis, leading enhanced tumor cell death. This review gives an insight about PDT/SDT limitations in need therapy. We try unveil process explore mechanism behind improved SPDT-meditated death cells by focusing on ROS-mediated response occurring result discussing current modifications existing sensitizers optimal use

Язык: Английский

Процитировано

4

MSC-derived extracellular vesicles: Precision miRNA delivery for overcoming cancer therapy resistance DOI
Ahsas Goyal, Muhammad Afzal,

Kavita Goyal

и другие.

Regenerative Therapy, Год журнала: 2025, Номер 29, С. 303 - 318

Опубликована: Апрель 5, 2025

Язык: Английский

Процитировано

0

Investigating Expression Dynamics of miR-21 and miR-10b in Glioblastoma Cells In Vitro: Insights into Responses to Hypoxia and Secretion Mechanisms DOI Open Access

Hanna Charbit,

Iris Lavon

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(14), С. 7984 - 7984

Опубликована: Июль 22, 2024

Glioblastoma poses significant challenges in oncology, with bevacizumab showing promise as an antiangiogenic treatment but limited efficacy. microRNAs (miRNAs) 10b and 21 have emerged potential biomarkers for response glioblastoma patients. This study delves into the expression dynamics of miR-21 miR-10b to hypoxia explores their circulation mechanisms. In vitro experiments exposed glioma cells (A172, U87MG, U251) human umbilical vein endothelial (HUVEC) hypoxic conditions (1% oxygen) 24 h, revealing heightened levels cells. Manipulating demonstrating a decrease VEGF alpha (VEGFA) following overexpression under conditions. Size exclusion chromatography illustrated notable shift towards exosomal packaging during hypoxia. A proposed model suggests that effective reduces VEGFA levels, heightening subsequently upregulating expression. These miRNAs, released via exosomes, might impact various cellular processes, notably contributing level reduction. However, post-treatment increases could potentially restore normoxic through downregulation VEGF. highlights intricate feedback loop involving miR-10b, miR-21, treatment, underscoring necessity personalized therapeutic strategies. Further research should explore clinical implications treatments.

Язык: Английский

Процитировано

1

Dormancy and awakening of cancer cells: the extracellular vesicle-mediated cross-talk between Dr. Jekill and Mr. Hyde DOI Creative Commons

Concetta D'Antonio,

Giovanna L. Liguori

Frontiers in Immunology, Год журнала: 2024, Номер 15

Опубликована: Сен. 5, 2024

Cancer cell dormancy is a reversible process whereby cancer cells enter quiescent state characterized by cycle arrest, inhibition of migration and invasion, increased chemoresistance. Because its reversibility resistance to treatment, key study, monitor, interfere with, in order prevent tumor recurrence metastasis improve the prognosis patients. However, achieve this goal, further studies are needed elucidate mechanisms underlying complex dynamic dual process. Here, we review contribution extracellular vesicles (EVs) regulation dormancy/awakening, focusing on cross-talk between non-tumor both primary (pre-)metastatic niche. Although EVs recognized as players progression metastasis, well diagnostics therapeutics, their role specifically induction/escape still largely elusive. We report most recent promising results topic, EV-associated nucleic acids involved. highlight how EV could greatly contribute identification signaling pathways dormancy/early awakening signature for development successful diagnostic/prognostic therapeutic approaches.

Язык: Английский

Процитировано

1

Implications of glioblastoma-derived exosomes in modifying the immune system: state-of-the-art and challenges DOI

Yashmin Afshar,

Negin Sharifi,

Amirhossein Kamroo

и другие.

Reviews in the Neurosciences, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 12, 2024

Glioblastoma is a brain cancer with poor prognosis. Failure of classical chemotherapy and surgical treatments indicates that new therapeutic approaches are needed. Among cell-free options, exosomes versatile extracellular vesicles (EVs) carry important cargo across barriers such as the blood-brain barrier (BBB) to their target cells. This makes an interesting option for treatment glioblastoma. Moreover, can comprise many cargos, including lipids, proteins, nucleic acids, sampled from special intercellular compartments origin cell. Cells exposed various immunomodulatory stimuli generate enriched in specific molecules. Notably, secretion could modify immune response innate adaptive systems. For instance, glioblastoma-associated (GBex) uptake by macrophages influence macrophage dynamics (e.g., shifting CD markers expression). Expression critical immunoregulatory proteins cytotoxic T-lymphocyte antigen-1 (CTLA1) programmed death-1 (PD-1) on GBex direct crosstalk these nano-size system. The present study reviews role system cells, B T natural killer (NK) dendritic cells (DCs), well novel technologies field.

Язык: Английский

Процитировано

0

Glioma Stem Cells: GPRC5A as a Novel Predictive Biomarker and Therapeutic Target Associated with Mesenchymal and Stemness Features DOI Creative Commons
Sara Sadat Aghamiri, Rada Amin

Applied Sciences, Год журнала: 2024, Номер 14(18), С. 8482 - 8482

Опубликована: Сен. 20, 2024

Glioblastoma multiforme (GBM) represents the deadliest form of brain cancer, characterized by complex interactions within its microenvironment. Despite understanding GBM biology, remains highly resistant to any therapy. Therefore, defining innovative biomarkers in can provide insights into tumor biology and potential therapeutic targets. In this study, we explored GPRC5A serve as a pertinent biomarker for GBM. We utilized GBM-TCGA dataset presented reproducible bioinformatics analysis our results. identified that expression was significantly upregulated compared normal tissues, with higher levels correlating poor overall survival (OS) progression-free interval (PFI). Moreover, it associated key genetic mutations, particularly NF1 PTEN strongly correlated mesenchymal stem-like phenotype. also predominantly aggressive features, including hypoxia, high extracellular matrix (ECM) environments, extensive stromal immune infiltrations. Its strong correlation markers hypoxic regions underscores target These findings valuable role pathology impact stratifications treatment strategies.

Язык: Английский

Процитировано

0