Cloning and characterisation of NMDA receptors in the Pacific oyster, Crassostrea gigas (Thunberg, 1793) in relation to metamorphosis and catecholamine synthesis DOI Creative Commons
Susanne Vogeler, Stefano Carboni, Xiaoxu Li

и другие.

Developmental Biology, Год журнала: 2020, Номер 469, С. 144 - 159

Опубликована: Окт. 22, 2020

Bivalve metamorphosis is a developmental transition from free-living larva to benthic juvenile (spat), regulated by complex interaction of neurotransmitters and neurohormones such as L-DOPA epinephrine (catecholamine). We recently suggested an N-Methyl-D-aspartate (NMDA) receptor pathway additional previously unknown regulator bivalve metamorphosis. To explore this theory further, we successfully induced in the Pacific oyster, Crassostrea gigas, exposing competent larvae L-DOPA, epinephrine, MK-801 ifenprodil. Subsequently, cloned three NMDA subunits CgNR1, CgNR2A CgNR2B, with sequence analysis suggesting successful assembly functional complexes binding natural occurring agonists channel blocker MK-801. are expressed larvae, during spat, but expression neither self-regulated nor catecholamines. In-situ hybridisation CgNR1 identified presence apical organ/cerebral ganglia area potential sensory function, nervous network foot indicating putative muscle regulatory function. Furthermore, phylogenetic analyses molluscan-specific gene expansions key enzymes involved catecholamine biosynthesis. However, exposure did not alter selected enzymes, that receptors do regulate biosynthesis catecholamines via expression.

Язык: Английский

Sex-dependent effects of the uncompetitive N-methyl-D-aspartate receptor antagonist REL-1017 in G93A-SOD1 amyotrophic lateral sclerosis mice DOI Creative Commons
Martina Colognesi, Atea Shkodra, Daniela Gabbia

и другие.

Frontiers in Neurology, Год журнала: 2024, Номер 15

Опубликована: Май 3, 2024

Introduction The pathogenesis of amyotrophic lateral sclerosis (ALS), a fatal neurodegenerative disease caused by the demise motor neurons has been linked to excitotoxicity excessive calcium influx via N-methyl-D-aspartate receptors (NMDARs), suggesting that uncompetitive NMDAR antagonism could be strategy attenuate neuron degeneration. REL-1017, dextro-isomer racemic methadone, is low-affinity antagonist. Importantly, in humans REL-1017 shown excellent tolerability clinical trials for major depression. Methods Here, we tested if improves phenotypes G93A SOD1 mouse, well-established model familial ALS, examining survival and functions, as well expression genes proteins involved neuroplasticity. Results We found sex-dependent effect mice. A delay ALS symptom onset, assessed 10%-decrease body weight ( p < 0.01 vs. control untreated mice) an extension lifespan 0.001 was observed male Female mice treated with showed improvement muscle strength mice). Both males females decrease hind limb clasping. Sex-dependent effects were also detected molecular markers neuronal plasticity (PSD95 SYN1) spinal cord GluN1 subunit quadricep muscles. Conclusion In conclusion, this study provides preclinical vivo evidence supporting evaluation ALS.

Язык: Английский

Процитировано

1
An α7 nicotinic and GABAB receptor‐mediated pathway controls acetylcholine release in the tripartite neuromuscular junction DOI Creative Commons
K. A. PETROV, O. A. Lenina,

Jacqueline Leroy

и другие.

The Journal of Physiology, Год журнала: 2024, Номер 603(2), С. 507 - 527

Опубликована: Дек. 30, 2024

Abstract Terminal Schwann cells (TSCs) are capable of regulating acetylcholine (ACh) release at the neuromuscular junction (NMJ). We have identified GABA as a gliotransmitter mouse NMJs. When ACh activates α7 nicotinic receptor (nAChRs) on TSCs, is released and B receptors nerve terminal that subsequently reduce release. Indeed, specific deletion nAChR in TSCs or inhibition metabotropic prevents reduction quantal content end‐plate potential induced by cholinesterase inhibitors. The α7/GABA receptor‐mediated pathway activated when escapes from collagen Q (ColQ) anchored AChE synaptic cleft PRiMA‐anchored butyrylcholinesterase TSC nAChRs TSC. Consequently, prolonged tetanic stimulation isolated muscle pathway, which reduces post‐tetanic levels low neonatal mice, decreases ex vivo fatigue. For ColQ‐deficient mice where not clustered, decrease AСh following activation this contributes to fatigue . image Key points Acetylcholine (NMJ) can activate (nAChR) cells, releasing gamma‐aminobutyric acid (GABA) then further nerve. At mature NMJ, before reaching normally hydrolyzed clustered BChE α7/GABAB depress subsequent NMJ low, either during development congenital myasthenic syndrome. In latter case,

Язык: Английский

Процитировано

1
Bivalves are NO different: nitric oxide as negative regulator of metamorphosis in the Pacific oyster, Crassostrea gigas DOI Creative Commons
Susanne Vogeler, Stefano Carboni, Xiaoxu Li

и другие.

BMC Developmental Biology, Год журнала: 2020, Номер 20(1)

Опубликована: Ноя. 23, 2020

Abstract Background Nitric oxide (NO) is presumed to be a regulator of metamorphosis in many invertebrate species, and although NO pathways have been comparatively well-investigated gastropods, annelids crustaceans, there has very limited research on the effects bivalve shellfish. Results In this paper, we investigate pathway inhibitors donors induction larvae Pacific oyster, Crassostrea gigas. The nitric oxides synthase (NOS) s-methylisothiourea hemisulfate salt (SMIS), aminoguanidine (AGH) 7-nitroindazole (7-NI) induced at 75, 76 83% respectively, operating concentration-dependent manner. Additional up 54% resulted from exposures 1H-[1,2,4]Oxadiazole[4,3-a]quinoxalin-1-one (ODQ), an inhibitor soluble guanylyl cyclase, with which interacts catalyse synthesis cyclic guanosine monophosphate (cGMP). Conversely, high concentrations donor sodium nitroprusside dihydrate combination inducers epinephrine, MK-801 or SMIS, significantly decreased metamorphosis, potential harmful effect excessive unrelated cannot excluded. Expression CgNOS also after regardless used, but intensified again post-metamorphosis spat. Fluorescent detection competent DAF-FM diacetate localisation oyster expression by in-situ hybridisation showed that occurs primarily two key larval structures, velum foot. cGMP was detected foot using immunofluorescent assays, potentially involved foot’s smooth muscle relaxation. Conclusion Together, these results suggest acts as negative larvae, reduction induces inhibiting swimming crawling behaviour, conjunction cascade additional neuroendocrine downstream responses.

Язык: Английский

Процитировано

9
Blockage of neuromuscular glutamate receptors impairs reinnervation following nerve crush in adult mice DOI Creative Commons
Kirkwood E. Personius,

Danielle Siebert,

Dennis W. Koch

и другие.

Frontiers in Cellular Neuroscience, Год журнала: 2022, Номер 16

Опубликована: Сен. 22, 2022

Motor axons in peripheral nerves are capable of regeneration following injury. However, complete recovery motor function is rare, particularly when reinnervation delayed. We have previously found that glutamate receptors play a crucial role the successful innervation muscle during mouse development. In particular, blocking N-methyl-D-aspartate (NMDA) receptor activity delays normal elimination excess each neuromuscular junction. Here, we use behavioral, immunohistochemical, electrophysiological, and calcium imaging methods to test whether similar transition from polyneuronal mono-innervation nerve injury mature muscle.

Язык: Английский

Процитировано

5
Cloning and characterisation of NMDA receptors in the Pacific oyster, Crassostrea gigas (Thunberg, 1793) in relation to metamorphosis and catecholamine synthesis DOI Creative Commons
Susanne Vogeler, Stefano Carboni, Xiaoxu Li

и другие.

Developmental Biology, Год журнала: 2020, Номер 469, С. 144 - 159

Опубликована: Окт. 22, 2020

Bivalve metamorphosis is a developmental transition from free-living larva to benthic juvenile (spat), regulated by complex interaction of neurotransmitters and neurohormones such as L-DOPA epinephrine (catecholamine). We recently suggested an N-Methyl-D-aspartate (NMDA) receptor pathway additional previously unknown regulator bivalve metamorphosis. To explore this theory further, we successfully induced in the Pacific oyster, Crassostrea gigas, exposing competent larvae L-DOPA, epinephrine, MK-801 ifenprodil. Subsequently, cloned three NMDA subunits CgNR1, CgNR2A CgNR2B, with sequence analysis suggesting successful assembly functional complexes binding natural occurring agonists channel blocker MK-801. are expressed larvae, during spat, but expression neither self-regulated nor catecholamines. In-situ hybridisation CgNR1 identified presence apical organ/cerebral ganglia area potential sensory function, nervous network foot indicating putative muscle regulatory function. Furthermore, phylogenetic analyses molluscan-specific gene expansions key enzymes involved catecholamine biosynthesis. However, exposure did not alter selected enzymes, that receptors do regulate biosynthesis catecholamines via expression.

Язык: Английский

Процитировано

6