Molecular Biomarkers and Signaling Pathways of Cancer Stem Cells in Colorectal Cancer

Technology in Cancer Research & Treatment, Год журнала: 2024, Номер 23

Опубликована: Янв. 1, 2024

Colorectal cancer (CRC) is the third most frequently found in world, and it discovered when already far along its development. About 20% of cases CRC are metastatic incurable. There more evidence that colorectal stem cells (CCSCs), which charge tumor growth, recurrence, resistance to treatment, what make so different. Because we know about cell biology, quickly learned molecular processes possible cross-talk between signaling pathways affect balance gut cancer. Wnt, Notch, TGF-β, Hedgehog examples pathway members whose genes may change produce CCSCs. These control self-renewal pluripotency SCs then decide function phenotype However, terms their ability create tumors susceptibility chemotherapeutic drugs, CSCs differ from normal bulk cells. This be reason for higher rate recurrence patients who underwent both surgery chemotherapy treatment. Scientists have a group uncontrolled miRNAs related CCSCs stemness properties. CCSC functions like changing expression cycle genes, metastasis, drug mechanisms. CCSC-related mostly signal known important biology. The biomarkers (CD markers miRNA) diagnostic roles main topics this review study.

Язык: Английский

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Non-Coding RNAs in Cancer: Structure, Function, and Clinical Application

Cancers, Год журнала: 2025, Номер 17(4), С. 579 - 579

Опубликована: Фев. 8, 2025

We are on the brink of a paradigm shift in both theoretical and clinical oncology. Genomic transcriptomic profiling, alongside personalized approaches that account for individual patient variability, increasingly shaping discourse. Discussions future cancer medicine mainly dominated by potential non-coding RNAs (ncRNAs), which play prominent role progression metastasis formation regulating expression oncogenic or tumor suppressor proteins at transcriptional post-transcriptional levels; furthermore, their cell-free counterparts might be involved intercellular communication. Non-coding considered to promising biomarker candidates early diagnosis as well therapeutic agents. This review aims provide clarity amidst vast body literature focusing diverse species ncRNAs, exploring structure, origin, function, applications miRNAs, siRNAs, lncRNAs, circRNAs, snRNAs, snoRNAs, eRNAs, paRNAs, YRNAs, vtRNAs, piRNAs. discuss molecular methods used detection functional studies vitro vivo. also address challenges must overcome enter new era therapy will reshape

Язык: Английский

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Whole-transcriptome sequencing in neural and non-neural tissues of a mouse model identifies miR-34a as a key regulator in SMA pathogenesis

Molecular Therapy — Nucleic Acids, Год журнала: 2025, Номер 36(2), С. 102490 - 102490

Опубликована: Фев. 20, 2025

Spinal muscular atrophy (SMA) is a severe neurodegenerative disorder caused by deficiency of survival motor neuron (SMN). While significant progress has been made in SMA therapy rescuing SMN expression, limited knowledge about downstream genes hindered the development alternative therapies. Here, we conducted whole-transcriptome sequencing spinal cord, heart, and liver tissues mouse model at early postnatal ages to explore critical coding non-coding RNAs (ncRNAs). A large number differentially expressed (DE-RNAs) were obtained, including 2,771 mRNAs, 382 microRNAs (miRNAs), 1,633 long ncRNAs, 1,519 circular RNAs. Through in-depth data mining, unveiled deregulation miR-34a all tissues. Analysis competitive endogenous RNA networks DE-RNAs identified multiple novel targets Spag5 mRNA, lncRNA00138536, circRNA007386. Further vitro studies using myoblast human cardiomyocyte cell lines showed that knockdown upregulated miR-34a-5p overexpression alone disrupted cell-cycle progression through regulating its targets, recapitulating gene expression patterns observed cardiac tissue mice. Our results miRNA involved pathology, which sheds insights into molecular basis widespread abnormalities forms SMA.

Язык: Английский

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Non-Coding RNAs Modulating Estrogen Signaling and Response to Endocrine Therapy in Breast Cancer

Cancers, Год журнала: 2023, Номер 15(6), С. 1632 - 1632

Опубликована: Март 7, 2023

The largest part of human DNA is transcribed into RNA that does not code for proteins. These non-coding RNAs (ncRNAs) are key regulators protein-coding gene expression and have been shown to play important roles in health, disease therapy response. Today, endocrine ERα-positive breast cancer (BC) a successful treatment approach, but resistance this major clinical problem. Therefore, deeper understanding mechanisms overcome resistance. An increasing amount evidence demonstrate ncRNAs affect the response therapy. Thus, considered versatile biomarkers predict or monitor In review article, we intend give summary update on effects microRNAs (miRNAs), long (lncRNAs) circular (circRNAs) estrogen signaling BC cells, pathway being target therapy, their role For purpose, reviewed articles these topics listed PubMed database. Finally, provide an assessment regarding use ncRNA types, particularly circulating forms, as predictive potential targets

Язык: Английский

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Bioinformatics analysis of long non-coding RNA-associated competing endogenous RNA network in schizophrenia

Scientific Reports, Год журнала: 2021, Номер 11(1)

Опубликована: Дек. 24, 2021

Abstract Schizophrenia (SCZ) is a serious psychiatric condition with 1% lifetime risk. SCZ one of the top ten global causes disabilities. Despite numerous attempts to understand function genetic factors in development, components pathophysiology remain unknown. The competing endogenous RNA (ceRNA) network has been demonstrated be involved development many kinds diseases. ceRNA hypothesis states that cross-talks between coding and non-coding RNAs, including long RNAs (lncRNAs), via miRNA complementary sequences known as response elements, creates large regulatory across transcriptome. In present study, we developed lncRNA-related elucidate molecular mechanisms SCZ. Microarray datasets associated brain regions (GSE53987) lymphoblasts (LBs) derived from peripheral blood (sample set B GSE73129) patients control subjects containing information about both mRNAs lncRNAs were downloaded Gene Expression Omnibus database. GSE53987 comprised 48 samples taken (15 HPC: hippocampus, 15 BA46: Brodmann area 46, 18 STR: striatum) 55 (18 HPC, 19 BA46, STR). sample GSE73129 30 LB controls). Differentially expressed (DEmRNAs) (DElncRNAs) identified using limma package R software. Using DIANA-LncBase, Human MicroRNA Disease Database (HMDD), miRTarBase, lncRNA- was generated. Pathway enrichment DEmRNAs performed Enrichr tool. We protein–protein interaction five hub genes by use STRING Cytoscape, respectively. Eventually, genes, DElncRNAs, predictive miRNAs chosen reconstruct subceRNA networks. Our bioinformatics analysis showed twelve key DEmRNAs, BDNF , VEGFA FGF2 FOS CD44 SOX2 NRAS SPARC ZFP36 FGG ELAVL1 STARD13 participate also DLX6-AS1 NEAT1 MINCR LINC01094 DLGAP1-AS1 BABAM2-AS1 PAX8-AS1 ZFHX4-AS1 XIST MALAT1 DElncRNAs regulating mentioned above. Furthermore, expression (e.g., ADM HLA-DRB1 ) DElncRNA ( changed LB, suggesting they could regarded candidates for future biomarker studies. study indicated ceRNAs research investigating pathways.

Язык: Английский

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Autophagy-Related Genes Predict the Progression of Periodontitis Through the ceRNA Network

Journal of Inflammation Research, Год журнала: 2022, Номер Volume 15, С. 1811 - 1824

Опубликована: Март 1, 2022

Purpose: The goal of this study was to identify the crucial autophagy-related genes (ARGs) in periodontitis and construct mRNA-miRNA-lncRNA networks further understand pathogenesis periodontitis. Methods: We used Gene Expression Omnibus (GEO) database Human Autophagy Database (HADb) differentially expressed mRNAs, miRNAs, ARGs. These ARGs were subjected Ontology (GO), KEGG (Kyoto Encyclopedia Genes Genomes) pathway, PPI (protein–protein interaction) network analysis. Two databases (miRDB StarBase v2.0) reverse-predict miRNAs while miRNA-lncRNA interaction predicted using v2.0 LncBase Predicted v.2 databases. After excluding lncRNAs only present nucleus, a competing endogenous RNA (ceRNA) built. Finally, we quantitative real-time PCR (qRT-PCR) confirm levels mRNA expression ceRNA network. Results: differential analysis revealed 10 upregulated downregulated intersecting reverse-predicted with consisting 4 mRNAs (LAMP2, NFE2L2, NCKAP1, EGFR), 3 (hsa-miR-140-3p, hsa-miR-142-5p, hsa-miR-671-5p), 30 constructed. In addition, qRT-PCR results that EGFR diseased gingival tissue patients. Conclusion: Four genes, especially EGFR, may play key role progression. novel aid elucidating mechanism autophagy periodontitis, which could be important developing new therapeutic options. Keywords: autophagy, ceRNA, bioinformatics analysis,

Язык: Английский

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Home and Away: The Role of Non-Coding RNA in Intracellular and Intercellular DNA Damage Response

Genes, Год журнала: 2021, Номер 12(10), С. 1475 - 1475

Опубликована: Сен. 23, 2021

Non-coding RNA (ncRNA) has recently emerged as a vital component of the DNA damage response (DDR), which was previously believed to be solely regulated by proteins. Many species ncRNA can directly or indirectly influence DDR and enhance repair, particularly in double-strand breaks, may hold therapeutic potential context cancer. These include long non-coding (lncRNA), microRNA, damage-induced lncRNA, small RNA, DNA:RNA hybrid structures, categorised cis trans based on location their synthesis relative sites. Mechanisms RNA-dependent recruitment scaffolding repair factors at break sites, regulation factor expression, stabilisation intermediates. also communicated intercellularly via exosomes, leading bystander responses healthy neighbour cells generate population-wide damage. microRNA have been implicated propagation damage, autophagy, radioresistance, prove significant for enhancing cancer treatment radiotherapy. Here, we review recent developments centred around contributions intracellular intercellular mechanisms.

Язык: Английский

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ceRNA Network Analysis Shows That lncRNA CRNDE Promotes Progression of Glioblastoma Through Sponge mir-9-5p

Frontiers in Genetics, Год журнала: 2021, Номер 12

Опубликована: Март 9, 2021

Glioblastoma accounts for 45.2% of central nervous system tumors. Despite the availability multiple treatments (e.g., surgery, radiotherapy, chemotherapy, biological therapy, immunotherapy, and electric field therapy), glioblastoma has a poor prognosis, with 5-year survival rate approximately 5%. The pathogenesis prognostic markers this cancer are currently unclear. To end, study aimed to explore identify potential markers. We used data from GEO TCGA databases identified five genes ( ITGA5 , MMP9 PTPRN PTX3 STX1A ) that could affect patients were differentially expressed between non-tumors groups. Based on variety bioinformatics tools reverse prediction target associated prognosis GBM, ceRNA network messenger RNA (STX1A, PTX3, MMP9)-microRNA (miR-9-5p)-long non-coding (CRNDE) was constructed. Finally, we therapeutic drugs (bacitracin, hecogenin, clemizole, chrysin, gibberellic acid) may be effective glioblastoma.

Язык: Английский

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Circular RNAs Modulate Cancer Hallmark and Molecular Pathways to Support Cancer Progression and Metastasis

Cancers, Год журнала: 2022, Номер 14(4), С. 862 - 862

Опубликована: Фев. 9, 2022

Circular RNAs (circRNAs) are noncoding products of backsplicing pre-mRNAs which have been established to possess potent biological functions. Dysregulated circRNA expression has linked diseases including different types cancer. Cancer progression is known result from the dysregulation several molecular mechanisms responsible for maintenance cellular and tissue homeostasis. The these processes defined as cancer hallmarks, pathways implicated in them regarded targets therapeutic interference. In this review, we summarize literature on investigation circRNAs hallmark signaling. First, present general information properties circRNAs, such their biogenesis degradation mechanisms, well basic Subsequently, roles framework each finally discuss potential targets.

Язык: Английский

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The function of LncRNA-ATB in cancer

Clinical & Translational Oncology, Год журнала: 2022, Номер 25(1), С. 1 - 9

Опубликована: Май 21, 2022

Язык: Английский

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