Structure and hepatoprotective activity of Usp10/NF-κB/Nrf2 pathway-related Morchella esculenta polysaccharide

Carbohydrate Polymers, Год журнала: 2022, Номер 303, С. 120453 - 120453

Опубликована: Дек. 13, 2022

Язык: Английский

---

Investigating the Link between Ketogenic Diet, NAFLD, Mitochondria, and Oxidative Stress: A Narrative Review

Antioxidants, Год журнала: 2023, Номер 12(5), С. 1065 - 1065

Опубликована: Май 8, 2023

Together with the global rise in obesity and metabolic syndrome, prevalence of individuals who suffer from nonalcoholic fatty liver disease (NAFLD) has risen dramatically. NAFLD is currently most common chronic includes a continuum disorders initial fat accumulation to steatohepatitis (NASH), considered more severe forms, which can evolve in, cirrhosis, hepatocellular carcinoma. Common features altered lipid metabolism mainly linked mitochondrial dysfunction, which, as vicious cycle, aggravates oxidative stress promotes inflammation and, consequence, progressive death hepatocytes form NAFLD. A ketogenic diet (KD), i.e., very low carbohydrates (<30 g/die) that induces “physiological ketosis”, been demonstrated alleviate restore function. Based on this, aim present review analyze body evidence regarding potential therapeutic role KD NAFLD, focusing interplay between mitochondria liver, effects ketosis pathways, impact

Язык: Английский

---

Exploring the impact of the PNPLA3 I148M variant on primary human hepatic stellate cells using 3D extracellular matrix models

Journal of Hepatology, Год журнала: 2024, Номер 80(6), С. 941 - 956

Опубликована: Фев. 15, 2024

Background and aims The PNPLA3 rs738409 C>G (encoding for I148M) variant is a risk locus the fibrogenic progression of chronic liver diseases, process driven by hepatic stellate cells (HSCs). We investigated how I148M affects HSCs biology using transcriptomic data validated findings in 3D-culture models. Methods RNA sequencing was performed on 2D-cultured primary human biopsies obese individuals, genotyped variant. Data were wild type (WT) or carrying I148M-PNPLA3 cultured 3D extracellular matrix (ECM) scaffolds from healthy cirrhotic with/without TGFB1 Cytosporone-B (Csn-B) treatment. Results Comparison between analysis highlighted shared I148M-driven dysregulated pathways related to mitochondrial function, antioxidant response, ECM remodelling signalling. Analogous WT/I148M-PNPLA3 scaffolds. Mitochondrial dysfunction linked respiratory chain complex IV insufficiency. Antioxidant capacity lower HSCs, while ROS secretion increased higher bioengineered versus signalling pathway followed same trend. In cells, TGFB1-endogenous inhibitor NR4A1 expression activation decreased: treatment with Csn-B agonist total but not regulation TGFB1/Csn-B Akt WT-PNPLA3 Erk HSCs. Conclusion have impaired dampening antifibrotic activity. These features are exacerbated ECM, highlighting dual impact fibrotic microenvironment progressive diseases. Impact implications Hepatic (HSCs) play key role associated disease. genetic mutation has been fibrogenesis, its needs further investigation. Here, comparative novel vitro model, we demonstrate cell behaviour, show that it cell's function as well gene NR4A1. Our publicly available data, platform our could facilitate discovery targets develop more effective treatments

Язык: Английский

---

Dual-Emissive Detection of ATP and Hypochlorite Ions for Monitoring Inflammation-Driven Liver Injury In Vitro and In Vivo

Analytical Chemistry, Год журнала: 2024, Номер 96(23), С. 9408 - 9415

Опубликована: Май 28, 2024

Reactive oxygen species play a pivotal role in liver disease, contributing to severe damage and chronic inflammation. In injury driven by inflammation, adenosine-5′-triphosphate (ATP) hypochlorite ion (ClO–) emerge as novel biomarkers, reflecting mitochondrial dysfunction amplified oxidative stress, respectively. However, the dynamic fluctuations of ATP ClO– hepatocytes mouse livers remain unclear, multidetection techniques for these biomarkers are yet be developed. This study presents RATP-NClO, dual-channel fluorescent bioprobe capable synchronously detecting ions. RATP-NClO exhibits excellent selectivity sensitivity ions, demonstrating fluorescence response murine hepatocyte cell line. Upon intravenous administration, reveals synchronized depletion amplification mice with experimental metabolic dysfunction-associated steatohepatitis (MASH). Through comprehensive analysis principal mechanism developed verification its reliable detection ability both vitro vivo settings, we propose it unique tool monitoring changes intracellular level. These findings underscore potential practical image-based functional phenotyping MASH pathogenesis.

Язык: Английский

---

Deciphering the molecular pathways of saroglitazar: A dual PPAR α/γ agonist for managing metabolic NAFLD

Metabolism, Год журнала: 2024, Номер 155, С. 155912 - 155912

Опубликована: Апрель 11, 2024

Язык: Английский

---

Improved Detection of Drug-Induced Liver Injury by Integrating Predicted In Vivo and In Vitro Data

Chemical Research in Toxicology, Год журнала: 2024, Номер 37(8), С. 1290 - 1305

Опубликована: Июль 9, 2024

Drug-induced liver injury (DILI) has been a significant challenge in drug discovery, often leading to clinical trial failures and necessitating withdrawals. Over the last decade, existing suite of

Язык: Английский

---

Si-Wu-Tang attenuates hepatocyte PANoptosis and M1 polarization of macrophages in non-alcoholic fatty liver disease by influencing the intercellular transfer of mtDNA

Journal of Ethnopharmacology, Год журнала: 2024, Номер 328, С. 118057 - 118057

Опубликована: Март 20, 2024

Язык: Английский

---

Pathogenesis of Hepatocellular Carcinoma: The Interplay of Apoptosis and Autophagy

Biomedicines, Год журнала: 2023, Номер 11(4), С. 1166 - 1166

Опубликована: Апрель 13, 2023

The pathogenesis of hepatocellular carcinoma (HCC) is a multifactorial process that has not yet been fully investigated. Autophagy and apoptosis are two important cellular pathways critical for cell survival or death. balance between autophagy regulates liver turnover maintains intracellular homeostasis. However, the often dysregulated in many cancers, including HCC. may be either independent parallel one influence other. inhibit promote apoptosis, thus regulating fate cancer cells. In this review, concise overview HCC presented, with emphasis on new developments, role endoplasmic reticulum stress, implication microRNAs gut microbiota. characteristics associated specific disease also described brief description provided. initiation, progress metastatic potential reviewed experimental evidence indicating an interplay extensively analyzed. ferroptosis, recently pathway regulated death, presented. Finally, therapeutic implications drug resistance examined.

Язык: Английский

---

The Roles of NFR2-Regulated Oxidative Stress and Mitochondrial Quality Control in Chronic Liver Diseases

Antioxidants, Год журнала: 2023, Номер 12(11), С. 1928 - 1928

Опубликована: Окт. 29, 2023

Chronic liver disease (CLD) affects a significant portion of the global population, leading to substantial number deaths each year. Distinct forms like non-alcoholic fatty (NAFLD) and alcoholic (ALD), though they have different etiologies, highlight shared pathologies rooted in oxidative stress. Central metabolism, mitochondria are essential for ATP production, gluconeogenesis, acid oxidation, heme synthesis. However, diseases NAFLD, ALD, fibrosis, mitochondrial function is compromised by inflammatory cytokines, hepatotoxins, metabolic irregularities. This dysfunction, especially electron leakage, exacerbates production reactive oxygen species (ROS), augmenting damage. Amidst this, nuclear factor erythroid 2-related 2 (NRF2) emerges as cellular protector. It not only counters stress regulating antioxidant genes but also maintains health overseeing autophagy biogenesis. The synergy between NRF2 modulation introduces new therapeutic potentials CLD, focusing on preserving integrity against threats. review delves into intricate role shedding light innovative strategies its prevention treatment, through pathways.

Язык: Английский

---

Multifaceted roles of aerobic glycolysis and oxidative phosphorylation in hepatocellular carcinoma

PeerJ, Год журнала: 2023, Номер 11, С. e14797 - e14797

Опубликована: Фев. 1, 2023

Liver cancer is a common malignancy with high morbidity and mortality rates. Changes in liver metabolism are key factors the development of primary hepatic carcinoma, mitochondrial dysfunction closely related to occurrence tumours. Accordingly, study metabolic mechanism mitochondria carcinomas has gained increasing attention. A growing body research suggests that defects respiration not generally responsible for aerobic glycolysis, nor they typically selected during tumour evolution. Conversely, oxidative phosphorylation (OXPHOS) may promote proliferation, metastasis, invasion carcinoma. This review presents current paradigm roles glycolysis OXPHOS hepatocellular carcinoma (HCC). Mitochondrial cytoplasmic cooperate maintain energy balance HCC cells. Our provides evidence targeting as potential therapy HCC.

Язык: Английский

---