Long COVID or Post-COVID-19 Condition: Past, Present and Future Research Directions

Microorganisms, Год журнала: 2023, Номер 11(12), С. 2959 - 2959

Опубликована: Дек. 11, 2023

The presence of symptoms after an acute SARS-CoV-2 infection (long-COVID) has become a worldwide healthcare emergency but remains underestimated and undertreated due to lack recognition the condition knowledge underlying mechanisms. In fact, prevalence post-COVID ranges from 50% during first months up 20% two-years after. This perspective review aimed map existing literature on identify gaps in guide global effort toward improved understanding long-COVID suggest future research directions. There is plethora symptomatology that can be COVID-19; however, today, there no clear classification definition this condition, termed or post-COVID-19 condition. heterogeneity led groups/clusters patients, which could exhibit different risk factors Viral persistence, long-lasting inflammation, immune dysregulation, autoimmune reactions, reactivation latent infections, endothelial dysfunction alteration gut microbiota have been proposed as potential mechanisms explaining complexity long-COVID. such equation, viral biology (e.g., re-infections, variants), host genetics, epigenetics) external vaccination) should also considered. These various will discussed current directions suggested.

Язык: Английский

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Oxidative Biomarkers Associated with the Pulmonary Manifestation of Post-COVID-19 Complications

Journal of Clinical Medicine, Год журнала: 2023, Номер 12(13), С. 4253 - 4253

Опубликована: Июнь 25, 2023

The role of mitochondria in post coronavirus disease 2019 (post-COVID-19) complications is unclear, especially the long-term pulmonary complications. This study aims to investigate association between post-COVID-19 and mitochondrial regulatory proteins context oxidative stress.Patients who had recovered from COVID-19 were enrolled. According evidence persistent interstitial lung lesions on computed tomography (CT), patients divided into a group (P(+)) control without (P(-)). We randomly selected 80 for investigation (40 subjects each group). Biomarkers levels determined by enzyme-linked immunosorbent assay (ELISA).The serum concentrations significantly higher P(+) group, including PTEN-induced kinase 1 (PINK1): 1.62 [1.02-2.29] ng/mL vs. 1.34 [0.94-1.74] (p = 0.046); Dynamin-1-like protein (DNM1L): 1.6 [0.9-2.4] IQR 0.9 [0.5-1.6] 0.004); Mitofusin-2 (MFN2): 0.3 [0.2-0.5] 0.2 [0.1-0.3] 0.001). Patients also chemokine ligand 18 (PARC, CCL18), IL-6, tumour necrosis factor-alpha (TNF-α) cytokines than P(-) group. concentration interferon alpha (IFN-α) was decreased Furthermore, we observed statistically significant correlations advanced glycation end product (sRAGE) TNF-α (Pearson's factor R 0.637; p < 0.001) DNM1L IFN-α 0.501; 0.002) patients.Elevated biomarkers with indicate their possible pathobiology sequelae. Oxidative stress associated immune response inflammation after COVID-19. could be promising biomarker predicting may potential target therapeutic intervention

Язык: Английский

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Expression of microRNAs in the Detection and Therapeutic Roles of Viral Infections: Mechanisms and Applications

Journal of Virus Eradication, Год журнала: 2025, Номер unknown, С. 100586 - 100586

Опубликована: Март 1, 2025

Язык: Английский

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A narrative review focusing on randomized clinical trials of vitamin D supplementation for COVID-19 disease

Frontiers in Nutrition, Год журнала: 2025, Номер 11

Опубликована: Янв. 7, 2025

Current evidence is inconsistent on whether vitamin D supplementation can prevent COVID-19 infection or improve its clinical outcomes. To better understand and look into the issue, we went through background knowledge of D, searched in Pubmed [by using key words title containing "randomized trial", "COVID-19", "vitamin (25-hydroxyvitamin cholecalciferol, calcidiol, calcifediol) supplementation"] for publications studies D/supplementation patients, especially those about randomized trials (RCTs). After reviewing these papers, did a short review pathophysiology COVID-19, summarized features 25 RCTs text tabulated table some features, commented, compared discussed differences between (for example, change serum 25-hydroxyvitamin concentration from nmol/L to ng/mL, making comparison easier). The take-home question that an important indicator effect correction but may not be reliable predicting outcomes COVID-19.

Язык: Английский

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Modulation of RAAS receptors and miRNAs in COVID-19: implications for disease severity, immune response, and potential therapeutic targets

BMC Infectious Diseases, Год журнала: 2025, Номер 25(1)

Опубликована: Март 24, 2025

The SARS-CoV-2 spike protein interacts with ACE2, a key receptor within the renin-angiotensin-aldosterone system (RAAS), which plays critical role in maintaining vascular homeostasis, regulating blood pressure, and modulating inflammation. An observational study analyzed gene expression profiles of RAAS receptors associated miRNAs 88 hospitalized COVID-19 patients 20 healthy controls, comparing acute post-acute phases to assess their impact on disease severity recovery. Our findings revealed an association between reduced MAS1 both advanced age (P = 0.03) need for oxygen supplementation 0.04). Additionally, ACE was worse mortality outcomes 0.01). Notably, ACE2 TMPRSS2 significantly decreased < 0.0001) individuals requiring those diabetes mellitus during post-COVID-19 phases, further highlighting these conditions RAAS. miRNA analysis significant downregulation miR-200c 0.005), miR-let-7 0.01), miR-122 acute-phase patients. This dysregulation contributes inflammatory response highlights interaction viral entry immune regulation. These results underscore significance ACE2/Ang-(1–7)/MAS1 axis inflammation regulation suggest that targeting this pathway may have therapeutic potential. provides valuable insights into molecular mechanisms pathogenesis identifies modulation as promising biomarkers potential interventions. Not applicable

Язык: Английский

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Dynamics of Gene Expression Profiling and Identification of High-Risk Patients for Severe COVID-19

Biomedicines, Год журнала: 2023, Номер 11(5), С. 1348 - 1348

Опубликована: Май 3, 2023

The clinical manifestations of SARS-CoV-2 infection vary widely, from asymptomatic to the development acute respiratory distress syndrome (ARDS) and death. host response elicited by plays a key role in determining outcome. We hypothesized that dynamic whole blood transcriptomic profile hospitalized adult COVID-19 patients characterizing subgroup develops severe disease ARDS would broaden our understanding heterogeneity outcomes. recruited 60 with RT-PCR-confirmed infection, among whom 19 developed ARDS. Peripheral was collected using PAXGene RNA tubes within 24 h admission on day 7. There were 2572 differently expressed genes at baseline 1149 found dysregulated inflammatory patients, an increased expression related pro-inflammatory molecules neutrophil macrophage activation admission, addition immune regulation loss. This led, turn, higher reactive oxygen species, protein polyubiquitination, metalloproteinases latter stages. Some most significant differences gene between without corresponded long non-coding involved epigenetic control.

Язык: Английский

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Altered expression of serum lncRNA CASC2 and miRNA-21-5p in COVID-19 patients

Human Genomics, Год журнала: 2024, Номер 18(1)

Опубликована: Фев. 12, 2024

Abstract Infection by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) that causes disease 2019 (COVID-19) has a high incidence of spread. On January 30, 2020, the World Health Organization proclaimed public health emergency worldwide concern. More than 6.9 million deaths and more 768 confirmed cases had been reported as June 18, 2023. This study included 51 patients 50 age- sex-matched healthy subjects. The present aimed to identify expression levels lncRNA CASC2 miRNA-21-5p (also known miRNA-21) in COVID-19 their relation clinicopathological characteristics disease. noncoding RNAs were measured RT-PCR technique. Results detected was significantly downregulated while upregulated compared A significant negative correlation found between miRNA-21-5p. ROC curve analysis used distinguish from controls. MiRNA-21-p serum level positive association with temperature PO2 ( p = 0.04 for each). These findings indicate miRNA-21-p might be potential diagnostic therapeutic biomarkers COVID-19.

Язык: Английский

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The impact of COVID-19 on microRNA and CD marker expression in AML patients

Scientific Reports, Год журнала: 2024, Номер 14(1)

Опубликована: Июнь 20, 2024

Abstract Acute myeloid leukaemia (AML) is an aggressive characterised by uncontrolled blast cell proliferation. miRNAs and Clusters of Differentiation (CD) molecules play essential roles in AML progression. This study aims to investigate the effect COVID-19 on expression circulating miRNA CD AML. cross-sectional recruited 32 patients 20 controls. Blood samples were collected analysed using molecular cytogenetic, miRNA/mRNA expression, flow cytometry techniques. The varied significantly between with control individuals. co-expression these was higher (P < 0.05), indicating that presence one led increased other miRNAs. A differential correlation observed markers. Additionally, 16, 21, 221 showed significant downregulation 0.05 P 0.01, respectively) infection compared those without a disease. Interestingly, this identified level 0.01) 137 as novel biomarker for patients. Moreover, high 0.05) most markers examined FISH features data. Furthermore, strong among positive negative infection. These data demonstrated contributed microRNAs MicroRNA microRNA exhibited differences healthy individuals, highlighting its role pathogenesis.

Язык: Английский

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The potential of circulating microRNAs as novel diagnostic biomarkers of COVID-19: a systematic review and meta-analysis

BMC Infectious Diseases, Год журнала: 2024, Номер 24(1)

Опубликована: Сен. 19, 2024

Язык: Английский

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Decoding the intricacies: a comprehensive analysis of microRNAs in the pathogenesis, diagnosis, prognosis and therapeutic strategies for COVID-19

Frontiers in Medicine, Год журнала: 2024, Номер 11

Опубликована: Окт. 7, 2024

The pandemic of coronavirus disease-19 (COVID-19), provoked by the appearance a novel named severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), required worldwide healthcare emergency. This has elicited an immediate need for accelerated research into its mechanisms disease, criteria diagnosis, methods forecasting outcomes, and treatment approaches. microRNAs (miRNAs), are diminutive RNA molecules, that non-coding participate in gene expression regulation post-transcriptionally, having important participation regulating immune processes. miRNAs have granted substantial interest their impact on viral replication, cell proliferation, modulation how host’s system responds. narrative review delves host miRNAs’ multifaceted roles within COVID-19 context, highlighting involvement disease progression, diagnostics, prognostics aspects, given stability biological fluids varied profiles when responding to infection. Additionally, we discuss complicated interactions between SARS-CoV-2 cellular machinery facilitated revealing dysregulation miRNA advances evasion, inflammatory responses. Furthermore, it investigates potential as therapeutic agents, whether synthetic or naturally occurring, which could be harnessed either mitigate harmful inflammation enhance antiviral However, searching more deeply is needed clarify involved pathogenesis COVID-19, diagnosis processes, prognostic assessments, approaches patients.

Язык: Английский

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