Simple Point-of-Care Nucleic Acid Amplification Test for Rapid SARS-CoV-2 Infection Diagnosis DOI Creative Commons
Hyunseul Jee,

Minkyeong Choi,

Insu Park

и другие.

Diagnostics, Год журнала: 2023, Номер 13(18), С. 3001 - 3001

Опубликована: Сен. 20, 2023

After three years of the SARS-CoV-2 pandemic, demand for developing field-deployable point-of-care (PoC) molecular diagnostic tests has increased. Although RT-qPCR is gold standard and accurate, it not readily applied to testing (POCT). Meanwhile, rapid kits have disadvantage low sensitivity. Recently, isothermal nucleic acid amplification technology emerged as an alternative diagnosis. Here, we developed a reverse transcription loop-mediated (RT-LAMP)-lateral flow assay (LFA) kit. This kit includes Chelex-100/boiling extraction device one-step detection apparatus capable performing entire process, from RNA detection, diagnosing infection within 40 min without contamination. The limits RT-LAMP-LFA were 100 plaque-forming units (PFUs) mL−1 10−1 PFU samples extracted using commercial AdvansureTM E3 system, respectively. sensitivity specificity 97.8% 100%, Our exhibited high requiring laboratory instruments, suggesting that could be used POC test SARS-CoV-2.

Язык: Английский

Paper-based point of care diagnostics for cancer biomarkers DOI Creative Commons
Prateek Bhardwaj, B Arora, Survanshu Saxena

и другие.

Sensors & Diagnostics, Год журнала: 2024, Номер 3(4), С. 504 - 535

Опубликована: Янв. 1, 2024

Advancements in assay design, detection techniques, signal transduction and enhancement strategies using smart nanomaterials.

Язык: Английский

Процитировано

10
CRISPR/Cas systems for genomic Editing, biochemical Sensing, Bioanalysis, and diagnostics DOI
Mirza Muhammad Faran Ashraf Baig, Sek Ying Chair, Wai Tong Chien

и другие.

Microchemical Journal, Год журнала: 2025, Номер unknown, С. 112638 - 112638

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

1
An overview of point-of-care testing for infections in critically ill patients DOI

Alena Moerman,

Jan J. De Waele, Jerina Boelens

и другие.

Expert Review of Molecular Diagnostics, Год журнала: 2024, Номер 24(3), С. 193 - 200

Опубликована: Фев. 27, 2024

Introduction Molecular diagnostic systems for point-of-care (POC) testing are nowadays routinely used and part of many labs. Although often intended bedside use outside the microbiology lab, there is still room expansion.

Язык: Английский

Процитировано

4
Current advances in Hepatitis C diagnostics DOI Creative Commons
Anna S. Baber, Baviththira Suganthan, Ramaraja P. Ramasamy

и другие.

Journal of Biological Engineering, Год журнала: 2024, Номер 18(1)

Опубликована: Сен. 9, 2024

Язык: Английский

Процитировано

4
Diagnosis of Infectious Diseases by CRISPR/Cas System DOI Open Access
Mahintaj Dara,

Negin Shafieipour,

Mahsa Saffar

и другие.

OBM Genetics, Год журнала: 2025, Номер 09(02), С. 1 - 39

Опубликована: Апрель 1, 2025

Since the initial discovery of CRISPR system in bacteria as an adaptive immune system, a deeper understanding structure and function has made it possible to perform gene editing, therapy, revolutionize diagnostic field. One exciting applications CRISPR-Cas is used tool for rapid diagnosis infectious diseases their treatment prevention infection spread among people. The CRISPR-based could be next-generation standard because its programmability capability searching target sequence quickly, making diagnose infections at lower cost but with same accuracy conventional methods. In this review, data were collected from valid papers published PubMed/Medline, Google Scholar, GISAID, Wiley Online Library, Web Science, ResearchGate databases. We have discussed novel various that routinely soon.

Язык: Английский

Процитировано

0
Detection of Parasites in the Field: The Ever-Innovating CRISPR/Cas12a DOI Creative Commons
Xin Li,

Zhisheng Dang,

Wenqiang Tang

и другие.

Biosensors, Год журнала: 2024, Номер 14(3), С. 145 - 145

Опубликована: Март 14, 2024

The rapid and accurate identification of parasites is crucial for prompt therapeutic intervention in parasitosis effective epidemiological surveillance. For clinical diagnosis, it imperative to develop a nucleic-acid-based diagnostic tool that combines the sensitivity specificity nucleic acid amplification tests (NAATs) with speed, cost-effectiveness, convenience isothermal methods. A new detection method, utilizing clustered regularly interspaced short palindromic repeats (CRISPR)-associated (Cas) nuclease, holds promise point-of-care testing (POCT). CRISPR/Cas12a presently employed

Язык: Английский

Процитировано

3
Establishment of LAMP-CRISPR/Cas12a for rapid detection of Escherichia coli O157:H7 and One-Pot detection DOI

Zuwei Wang,

Huan Chen,

Antuo Hu

и другие.

Food Microbiology, Год журнала: 2024, Номер 124, С. 104622 - 104622

Опубликована: Авг. 24, 2024

Язык: Английский

Процитировано

3
Dual Detection of Hepatitis B and C Viruses Using CRISPR‐Cas Systems and Lateral Flow Assay DOI Creative Commons
Syeda Najidah Shahni, Sarah Albogami, Iqbal Azmi

и другие.

Journal of Nucleic Acids, Год журнала: 2024, Номер 2024(1)

Опубликована: Янв. 1, 2024

The development of sensitive and specific diagnostic tools for hepatitis B virus (HBV) C (HCV) remains crucial effective disease management control. In this study, we utilized CRISPR‐Cas12 CRISPR‐Cas13 systems the detection HBV (DNA virus) HCV (RNA virus), respectively. We designed tested multiple guide RNAs (gRNAs) targeting both viruses, confirming successful cleavage target sequences through gel electrophoresis a fluorescent reporter assay. Using optimized gRNAs, developed lateral flow assay (LFA) HCV, demonstrating concentration‐dependent signal increase. Importantly, no cross‐reactivity was observed with other viral targets. To further enhance sensitivity, employed dual‐enzyme approach, combining Cas12 Cas13 in single reaction, which significantly improved limits viruses. Finally, dual antigen LFA strip capable simultaneously detecting sample. This approach holds promise point‐of‐care (POC) diagnostics where infection is unknown. study addresses current limitations CRISPR‐Cas based diagnostics, namely, need ultrasensitive methods ability to detect antigens using test strip. Our findings demonstrate feasibility highly paving way potential POC application.

Язык: Английский

Процитировано

3
Simple Point-of-Care Nucleic Acid Amplification Test for Rapid SARS-CoV-2 Infection Diagnosis DOI Creative Commons
Hyunseul Jee,

Minkyeong Choi,

Insu Park

и другие.

Diagnostics, Год журнала: 2023, Номер 13(18), С. 3001 - 3001

Опубликована: Сен. 20, 2023

After three years of the SARS-CoV-2 pandemic, demand for developing field-deployable point-of-care (PoC) molecular diagnostic tests has increased. Although RT-qPCR is gold standard and accurate, it not readily applied to testing (POCT). Meanwhile, rapid kits have disadvantage low sensitivity. Recently, isothermal nucleic acid amplification technology emerged as an alternative diagnosis. Here, we developed a reverse transcription loop-mediated (RT-LAMP)-lateral flow assay (LFA) kit. This kit includes Chelex-100/boiling extraction device one-step detection apparatus capable performing entire process, from RNA detection, diagnosing infection within 40 min without contamination. The limits RT-LAMP-LFA were 100 plaque-forming units (PFUs) mL−1 10−1 PFU samples extracted using commercial AdvansureTM E3 system, respectively. sensitivity specificity 97.8% 100%, Our exhibited high requiring laboratory instruments, suggesting that could be used POC test SARS-CoV-2.

Язык: Английский

Процитировано

3