Simple Point-of-Care Nucleic Acid Amplification Test for Rapid SARS-CoV-2 Infection Diagnosis DOI Creative Commons
Hyunseul Jee,

Minkyeong Choi,

Insu Park

et al.

Diagnostics, Journal Year: 2023, Volume and Issue: 13(18), P. 3001 - 3001

Published: Sept. 20, 2023

After three years of the SARS-CoV-2 pandemic, demand for developing field-deployable point-of-care (PoC) molecular diagnostic tests has increased. Although RT-qPCR is gold standard and accurate, it not readily applied to testing (POCT). Meanwhile, rapid kits have disadvantage low sensitivity. Recently, isothermal nucleic acid amplification technology emerged as an alternative diagnosis. Here, we developed a reverse transcription loop-mediated (RT-LAMP)-lateral flow assay (LFA) kit. This kit includes Chelex-100/boiling extraction device one-step detection apparatus capable performing entire process, from RNA detection, diagnosing infection within 40 min without contamination. The limits RT-LAMP-LFA were 100 plaque-forming units (PFUs) mL−1 10−1 PFU samples extracted using commercial AdvansureTM E3 system, respectively. sensitivity specificity 97.8% 100%, Our exhibited high requiring laboratory instruments, suggesting that could be used POC test SARS-CoV-2.

Language: Английский

Paper-based point of care diagnostics for cancer biomarkers DOI Creative Commons
Prateek Bhardwaj, B Arora, Survanshu Saxena

et al.

Sensors & Diagnostics, Journal Year: 2024, Volume and Issue: 3(4), P. 504 - 535

Published: Jan. 1, 2024

Advancements in assay design, detection techniques, signal transduction and enhancement strategies using smart nanomaterials.

Language: Английский

Citations

10
CRISPR/Cas systems for genomic Editing, biochemical Sensing, Bioanalysis, and diagnostics DOI
Mirza Muhammad Faran Ashraf Baig, Sek Ying Chair, Wai Tong Chien

et al.

Microchemical Journal, Journal Year: 2025, Volume and Issue: unknown, P. 112638 - 112638

Published: Jan. 1, 2025

Language: Английский

Citations

1
An overview of point-of-care testing for infections in critically ill patients DOI

Alena Moerman,

Jan J. De Waele, Jerina Boelens

et al.

Expert Review of Molecular Diagnostics, Journal Year: 2024, Volume and Issue: 24(3), P. 193 - 200

Published: Feb. 27, 2024

Introduction Molecular diagnostic systems for point-of-care (POC) testing are nowadays routinely used and part of many labs. Although often intended bedside use outside the microbiology lab, there is still room expansion.

Language: Английский

Citations

4
Current advances in Hepatitis C diagnostics DOI Creative Commons
Anna S. Baber, Baviththira Suganthan, Ramaraja P. Ramasamy

et al.

Journal of Biological Engineering, Journal Year: 2024, Volume and Issue: 18(1)

Published: Sept. 9, 2024

Language: Английский

Citations

4
Diagnosis of Infectious Diseases by CRISPR/Cas System DOI Open Access
Mahintaj Dara,

Negin Shafieipour,

Mahsa Saffar

et al.

OBM Genetics, Journal Year: 2025, Volume and Issue: 09(02), P. 1 - 39

Published: April 1, 2025

Since the initial discovery of CRISPR system in bacteria as an adaptive immune system, a deeper understanding structure and function has made it possible to perform gene editing, therapy, revolutionize diagnostic field. One exciting applications CRISPR-Cas is used tool for rapid diagnosis infectious diseases their treatment prevention infection spread among people. The CRISPR-based could be next-generation standard because its programmability capability searching target sequence quickly, making diagnose infections at lower cost but with same accuracy conventional methods. In this review, data were collected from valid papers published PubMed/Medline, Google Scholar, GISAID, Wiley Online Library, Web Science, ResearchGate databases. We have discussed novel various that routinely soon.

Language: Английский

Citations

0
Detection of Parasites in the Field: The Ever-Innovating CRISPR/Cas12a DOI Creative Commons
Xin Li,

Zhisheng Dang,

Wenqiang Tang

et al.

Biosensors, Journal Year: 2024, Volume and Issue: 14(3), P. 145 - 145

Published: March 14, 2024

The rapid and accurate identification of parasites is crucial for prompt therapeutic intervention in parasitosis effective epidemiological surveillance. For clinical diagnosis, it imperative to develop a nucleic-acid-based diagnostic tool that combines the sensitivity specificity nucleic acid amplification tests (NAATs) with speed, cost-effectiveness, convenience isothermal methods. A new detection method, utilizing clustered regularly interspaced short palindromic repeats (CRISPR)-associated (Cas) nuclease, holds promise point-of-care testing (POCT). CRISPR/Cas12a presently employed

Language: Английский

Citations

3
Establishment of LAMP-CRISPR/Cas12a for rapid detection of Escherichia coli O157:H7 and One-Pot detection DOI

Zuwei Wang,

Huan Chen,

Antuo Hu

et al.

Food Microbiology, Journal Year: 2024, Volume and Issue: 124, P. 104622 - 104622

Published: Aug. 24, 2024

Language: Английский

Citations

3
Dual Detection of Hepatitis B and C Viruses Using CRISPR‐Cas Systems and Lateral Flow Assay DOI Creative Commons
Syeda Najidah Shahni, Sarah Albogami, Iqbal Azmi

et al.

Journal of Nucleic Acids, Journal Year: 2024, Volume and Issue: 2024(1)

Published: Jan. 1, 2024

The development of sensitive and specific diagnostic tools for hepatitis B virus (HBV) C (HCV) remains crucial effective disease management control. In this study, we utilized CRISPR‐Cas12 CRISPR‐Cas13 systems the detection HBV (DNA virus) HCV (RNA virus), respectively. We designed tested multiple guide RNAs (gRNAs) targeting both viruses, confirming successful cleavage target sequences through gel electrophoresis a fluorescent reporter assay. Using optimized gRNAs, developed lateral flow assay (LFA) HCV, demonstrating concentration‐dependent signal increase. Importantly, no cross‐reactivity was observed with other viral targets. To further enhance sensitivity, employed dual‐enzyme approach, combining Cas12 Cas13 in single reaction, which significantly improved limits viruses. Finally, dual antigen LFA strip capable simultaneously detecting sample. This approach holds promise point‐of‐care (POC) diagnostics where infection is unknown. study addresses current limitations CRISPR‐Cas based diagnostics, namely, need ultrasensitive methods ability to detect antigens using test strip. Our findings demonstrate feasibility highly paving way potential POC application.

Language: Английский

Citations

3
Simple Point-of-Care Nucleic Acid Amplification Test for Rapid SARS-CoV-2 Infection Diagnosis DOI Creative Commons
Hyunseul Jee,

Minkyeong Choi,

Insu Park

et al.

Diagnostics, Journal Year: 2023, Volume and Issue: 13(18), P. 3001 - 3001

Published: Sept. 20, 2023

After three years of the SARS-CoV-2 pandemic, demand for developing field-deployable point-of-care (PoC) molecular diagnostic tests has increased. Although RT-qPCR is gold standard and accurate, it not readily applied to testing (POCT). Meanwhile, rapid kits have disadvantage low sensitivity. Recently, isothermal nucleic acid amplification technology emerged as an alternative diagnosis. Here, we developed a reverse transcription loop-mediated (RT-LAMP)-lateral flow assay (LFA) kit. This kit includes Chelex-100/boiling extraction device one-step detection apparatus capable performing entire process, from RNA detection, diagnosing infection within 40 min without contamination. The limits RT-LAMP-LFA were 100 plaque-forming units (PFUs) mL−1 10−1 PFU samples extracted using commercial AdvansureTM E3 system, respectively. sensitivity specificity 97.8% 100%, Our exhibited high requiring laboratory instruments, suggesting that could be used POC test SARS-CoV-2.

Language: Английский

Citations

3