Overexpressed KCNK1 regulates potassium channels affecting molecular mechanisms and biological pathways in bladder cancer

European journal of medical research, Год журнала: 2024, Номер 29(1)

Опубликована: Апрель 30, 2024

Abstract Background This study aimed to explore the expression, molecular mechanism and its biological function of potassium two pore domain channel subfamily K member 1 (KCNK1) in bladder cancer (BC). Methods We integrated large numbers external samples (n = 1486) assess KCNK1 mRNA expression levels collected in-house 245) for immunohistochemistry (IHC) experiments validate at protein level. Single-cell RNA sequencing (scRNA-seq) analysis was performed further cellular communication. The transcriptional regulatory mechanisms were explored by ChIP-seq, ATAC-seq ChIA-PET data. Highly expressed co-expressed genes (HECEGs) used potential signalling pathways. Furthermore, immunoassay, clinical significance docking calculated. Results significantly overexpressed BC (SMD 0.58, 95% CI [0.05; 1.11]), validated level (p < 0.0001). Upregulated exhibited highly distinguishing ability between control (AUC 0.82 [0.78–0.85]). Further, scRNA-seq revealed that predominantly clustered epithelial cells tended increase with differentiation. involved communication mainly through MK pathway. Secondly, transcription start site (TSS) showed promoter-enhancer interactions three-dimensional space, while being transcriptionally regulated GRHL2 FOXA1. Most HECEGs enriched cell cycle–related metabolism–related pathways membrane activity. associated infiltration various immune cells. Immunotherapy chemotherapy (docetaxel, paclitaxel vinblastine) more effective patients high group. correlated age, pathology grade pathologic_M patients. Conclusions BC. A complex sophisticated spatial network existed TSS promoted upregulated expression. might be cycle, metabolism, tumour microenvironment regulation channels, ultimately contributed deterioration

Язык: Английский

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ALYREF m5C RNA methylation reader predicts bladder cancer prognosis by regulating the tumor immune microenvironment

Medicine, Год журнала: 2024, Номер 103(14), С. e37590 - e37590

Опубликована: Апрель 5, 2024

Background: 5-Methylcytidine (m5C) methylation is a recently emerging epigenetic modification that closely related to tumor proliferation, occurrence, and metastasis. This study aimed investigate the clinicopathological characteristics prognostic value of m5C regulators in bladder cancer (BLCA), their correlation with immune microenvironment. Methods: Thirteen RNA were analyzed using RNA-sequencing corresponding clinical information obtained from TCGA database. The Cluster Profiler package was used analyze gene ontology function potential targets enriched Kyoto Encyclopedia Genes Genomes pathway. Kaplan–Meier survival analysis compare differences log-rank test univariate Cox proportional hazards regression. between signature various cells analyzed. Univariate multivariate regression analyses identified independence ALYREF signature. Results: Nine out 13 differentially expressed BLCA normal samples co-expressed. These 9 associated characteristics, particularly high or low risk, pT pTNM stage, migration. Consensus clustering divides into 4 clusters. (KEGG) pathway enrichment annotation 273 upregulated 594 downregulated genes BLCA. Notably, only significantly correlated OS ( P < .05). exhibited significant infiltration levels macrophage cells. Therefore, we constructed nomogram for as an independent factor. Additionally, observed both mRNA protein upregulated, immunofluorescence showed mainly distributed nuclear speckles. overexpression poor OS. Conclusion: Our findings demonstrated predict risks patients regulate As such, may serve novel indicator patients.

Язык: Английский

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An Unusual Presentation of Bladder Carcinoma in a Visceral Hernia: A Case Report and Literature Review

Cancer Reports, Год журнала: 2025, Номер 8(2)

Опубликована: Фев. 1, 2025

Bladder carcinoma (BC) is the most prevalent malignancy of urinary system. These cancers are primarily seen in adults > 60 years old and mostly present with microscopic or frank hematuria obstruction However, these rare can be found hernias. This report discusses a rare, localized bladder urothelial (UC) manifestation. The patient had presented lower abdominal pain several times. no accurate diagnosis was made due to unspecified features. After being referred radiologic evaluation ultrasonography, hernia detected entering wall, it contained an unusual mass. Further evaluations revealed malignant feature tumor. wall replaced, TURP procedure performed. resulting sample showed UC without involvement muscle layer. One common malignancies urogenital reproductive systems male patients BCs. They commonly men older than history smoking. manifestations cancer macroscopic hematuria, obstruction, pain. A but previously reported location within inguinal this not always straightforward, delays result spread transition patient's clinical condition poorer prognosis. presentation accompanied by typical symptoms such as obstruction. Patients persistent should evaluated rule out malignancy. tumors might hidden hernias, more accuracy demanded for their diagnosis.

Язык: Английский

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A machine learning-based prognostic signature utilizing MSC proteomics for predicting bladder cancer prognosis and treatment response

Translational Oncology, Год журнала: 2025, Номер 54, С. 102349 - 102349

Опубликована: Март 11, 2025

Язык: Английский

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Diagnostic performance of advanced large language models in cystoscopy: evidence from a retrospective study and clinical cases

BMC Urology, Год журнала: 2025, Номер 25(1)

Опубликована: Март 29, 2025

To evaluate the diagnostic capabilities of advanced large language models (LLMs) in interpreting cystoscopy images for identification common urological conditions. A retrospective analysis was conducted on 603 obtained from 101 procedures. Two LLMs, both at forefront artificial intelligence technology, were employed to interpret these images. The interpretations generated by LLMs systematically compared against standard clinical assessments. study's primary outcome measure overall accuracy LLMs. Secondary outcomes focused evaluating condition-specific accuracies across various combined 89.2%, with ChatGPT-4 V and Claude 3.5 Sonnet achieving 82.8% 79.8%, respectively. Condition-specific varied considerably, specific disorders: bladder tumors (ChatGPT-4 V: 92.2%, Sonnet: 80.9%), BPH (35.3%, 32.4%), cystitis (94.5%, 98.9%), diverticula (92.3%, 53.8%), trabeculae (55.8%, 59.6%). As normal anatomical structures: ureteral orifice 48.8%, 61.0%), neck (97.9%, 93.8%), prostatic urethra (64.3%,57.1%). Advanced demonstrated varying levels image interpretation, excelling detection while showing lower other conditions, notably benign hyperplasia. These findings suggest promising potential as supportive tools diagnosis, particularly urologists training or early career stages. This study underscores need continued research development optimize AI-driven tools, ultimate goal improving efficiency practice. Not applicable.

Язык: Английский

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The Association between Tea Consumption and Bladder Cancer Risk Based on the Bladder Cancer Epidemiology and Nutritional Determinants (BLEND) International Consortium

Nutrition and Cancer, Год журнала: 2025, Номер unknown, С. 1 - 12

Опубликована: Апрель 8, 2025

Evidence regarding the association between tea consumption and bladder cancer (BC) risk is inconsistent. This study aimed to increase our knowledge of by using international data from Bladder Cancer Epidemiology Nutritional Determinants Consortium. Individual on 2,347 cases 6,871 controls 15 case-control studies with information black, green, herbal, or general was pooled. The estimated multilevel multivariable logistic regression analysis adjusted for multiple (non-)dietary factors. Association BC observed (odds ratio, OR = 0.72, 95% confidence interval, CI 0.65-0.80) compared non-tea drinkers. Stratified analyses based gender smoking status yielded similar results. showed no significant black green across models, while herbal linked a reduced (OR 0.59, 0.36-0.96). As daily increased within suitable range (<5.67 cups/day), decreased. Camellia sinensis risk, inversely incidence. Despite some findings in selected strata, further are required clarify underlying mechanisms.

Язык: Английский

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Usability Evaluation of Urinary HAI-1, STMN-1 and TN-C in the Diagnosis of Bladder Cancer

Journal of Clinical Medicine, Год журнала: 2025, Номер 14(11), С. 3664 - 3664

Опубликована: Май 23, 2025

Background: Proteins with different functions, such as Hepatocyte growth factor activator inhibitor type 1 (HAI-1), Stathmin (STMN-1), and Tenascin C (TN-C), whose activity has been observed in various types of cancers, inspired our study bladder cancer (BC) patients. The aim the was to evaluate selected parameters their combinations diagnosis BC. took into account degree invasiveness malignancy Based on analysis Receiver Operating Characteristic Curve (ROC), diagnostic value single potential indicators BC assessed. Patients Methods: research material consisted urine samples from patients BC, a control group without urological diseases. concentrations examined were measured using an immunoenzymatic method. Results: Statistically significant higher HAI-1 (p ≤ 0.001), STMN-1 (≤0.001) TN-C (0.002) found compared group. Strong relationships shown between these parameters. ROC analyses showed that best parameter for detecting is STMN-1, combination HAI-1+STMN-1. highest obtained HAI-1+STMN-1 high (sensitivity 82%, specificity 91%). Conclusions: Preliminary studies have utility markers especially However, learn more about contribution progression cancer, it would be appropriate continue studies.

Язык: Английский

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The use of ctDNA for muscle-invasive bladder cancer before and after radical cystectomy

Expert Review of Anticancer Therapy, Год журнала: 2025, Номер unknown

Опубликована: Май 26, 2025

Muscle-invasive bladder cancer (MIBC) is an aggressive malignancy with high recurrence rates despite standard treatment radical cystectomy neoadjuvant chemotherapy. Conventional imaging and histopathology have limited ability to detect minimal residual disease (MRD) or predict response. Circulating tumor DNA (ctDNA), has emerged as a promising noninvasive biomarker for dynamic monitoring risk stratification. This review explores the current emerging applications of ctDNA in MIBC, including its use adjuvant settings, MRD detection, surveillance, guidance. Key clinical trials, such IMvigor010/011, ABACUS, NABUCCO, TOMBOLA, MODERN, VOLGA are discussed, highlighting prognostic predictive value across stages. Technical biological limitations examined, along challenges related standardization, cost, integration. poised transform perioperative management MIBC by enabling precision-guided decisions. assays offer sensitivity detection early relapse prediction. While not yet part routine practice, ongoing studies will determine whether ctDNA-guided interventions improve patient outcomes. With continued advancements assay sensitivity, bioinformatics, integration radiomic genomic data, expected become cornerstone urothelial carcinoma.

Язык: Английский

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Non-invasive urinary biomarkers in the diagnosis and monitoring of bladder cancer

Опубликована: Май 22, 2025

Язык: Английский

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Liquid biopsy for promoter methylation in prostate cancer: a promising non-invasive diagnostic weapon

World Journal of Urology, Год журнала: 2025, Номер 43(1)

Опубликована: Июнь 3, 2025

Язык: Английский

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