Pathogens, Год журнала: 2024, Номер 13(10), С. 892 - 892
Опубликована: Окт. 11, 2024
Mosquitoes serve as vectors for many arthropod-borne viruses (arboviruses) that are responsible millions of human infections and thousands deaths each year. Among these arboviruses, O'nyong-nyong virus (ONNV) is an African alphavirus mainly transmitted by
Язык: Английский
Functional & Integrative Genomics, Год журнала: 2024, Номер 24(1)
Опубликована: Янв. 20, 2024
Язык: Английский
Процитировано
6
Journal of Virological Methods, Год журнала: 2025, Номер unknown, С. 115156 - 115156
Опубликована: Апрель 1, 2025
Язык: Английский
Процитировано
0
Diagnostics, Год журнала: 2024, Номер 14(2), С. 221 - 221
Опубликована: Янв. 19, 2024
COVID-19 made explicit the need for rethinking way in which we conduct testing epidemic emergencies. During pandemic, dependence on centralized lab facilities and resource-intensive methodologies (e.g., RT-qPCR methods) greatly limited deployment of widespread efforts many developed underdeveloped countries. Here, illustrate development a simple portable diagnostic kit that enables self-diagnosis at home from saliva samples. We describe do-it-yourself (DIY) incubator Eppendorf tubes can be used to SARS-CoV-2 detection with competitive sensitivity selectivity home. In proof-of-concept experiment, assembled Eppendorf-tube incubators our shop, prepared single-tube mix reagents LAMP primers lab, deployed these kits using urban delivery systems (i.e., Rappifavor or Uber) more than 15 different locations Monterrey, México. This straightforward strategy enabled rapid cost-effective at-home molecular diagnostics real samples high (100%) (87%).
Язык: Английский
Процитировано
2
Food Control, Год журнала: 2024, Номер 163, С. 110546 - 110546
Опубликована: Апрель 26, 2024
Язык: Английский
Процитировано
2
Frontiers in Lab on a Chip Technologies, Год журнала: 2024, Номер 3
Опубликована: Авг. 27, 2024
Decentralized Point-of-Care (PoC) diagnostics hold momentous potential for rapid and accessible viral infection disease detection. Presented is a unique design application of an easy-to-use (plug-and-play) platform The leverages simplified multiplex Reverse-Transcription Loop-mediated Isothermal Amplification (RT-LAMP) Lateral Flow Biosensor (LFB) assay with lyophilized master mix, eliminating the need RNA isolation or special reporting equipment. A user-friendly Saliva Measuring Tube (SMT) ensures accurate saliva volume self-collection, Syringe-based PoC (SPoC) automates sample treatment, reagent mixing, temperature control using readily available components consumables. platform’s performance was evaluated by multiplexed detection SARS-CoV-2 N2 target gene human ACTB from samples. SPoC achieved limit spiked 500 copies/mL consistent internal readout. presented system offers promising initial step further development toward decentralized solution testing.
Язык: Английский
Процитировано
2
Scientific Reports, Год журнала: 2024, Номер 14(1)
Опубликована: Сен. 28, 2024
Язык: Английский
Процитировано
1
Biomedicines, Год журнала: 2023, Номер 11(9), С. 2344 - 2344
Опубликована: Авг. 23, 2023
The goal of this study was to evaluate the performance a commercial reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay (Detect COVID-19 Test) in detection severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). A total 202 human and viral culture specimens were tested retrospectively. Detect Test comparable that real-time polymerase chain reaction assays (sensitivity: 93.42%; specificity: 100%), better than rapid antigen test 48.00%; 100%) for with threshold cycle (Ct) values less 30. Beta, Delta, Omicron variants concern successfully detected. With their simplicity use good sensitivity, point-of-care RT-LAMP may be viable option SARS-CoV-2 testing at home, or regions without sophisticated laboratory facilities.
Язык: Английский
Процитировано
1
Cureus, Год журнала: 2024, Номер unknown
Опубликована: Фев. 19, 2024
Coronavirus disease 2019 (COVID-19) continues to be a global health threat and is public issue in Thailand other countries. The extensive cross-border between Myanmar considered at potentially high risk for COVID-19 distribution this region. In instance, simple cost-effective tests rapid early detection of would useful effective patient management control the disease.
Язык: Английский
Процитировано
0
Опубликована: Янв. 1, 2024
Background: Reverse-transcriptase loop-mediated isothermal amplification (RT-LAMP) is a rapid, robust, simple, and cost-effective one-step assay for the identification of SARS-CoV-2 RNA in nasopharyngeal (NP) swabs. Herein, we demonstrate expansion RT-LAMP Africa its operational performance directly from saliva as pragmatic approach outbreak surveillance response resource-limited settings Africa.Methods: A multi-country diagnostic trial was conducted between September 2021 June 2022 on 2774 NP swabs seven countries (Angola, Burkina-Faso, Ivory Coast, Ethiopia, Senegal, Sudan, Zimbabwe). In parallel, 577 samples were tested with (without extraction) four (Cameroon, Kenya, Nigeria). both contexts, real-time quantitative polymerase chain reaction (RT-qPCR) using extracted used gold-standard benchmarking to evaluate RT-LAMP.Findings: The overall sensitivity specificity across 89% (95%CI: 87-90%) 95% 93-96%), respectively, positive predictive value (PPV) 94-96%) negative (NPV) 88% 86-89%). Similarly, applied without extraction showed 80% 75-84%) sensitivity, 99% 96-100%) specificity, (95% CI: 97-100) PPV 77% 77-82) NPV.Interpretation: Colorimetric reliable detection crude samples. Its good underscores scalability efficient settings.Funding: This work supported, whole or part, by Bill & Melinda Gates Foundation [Grant No. INV-034,799].Declaration Interest: authors declare that this study supported INV-034,799]. Furthermore, New England Biolabs provided reagents free charge. have no additional interests declare.Ethical Approval: Ethical approvals obtained each country, detailed Supplementary Tables 2 3.
Язык: Английский
Процитировано
0
Journal of Indian Society of Periodontology, Год журнала: 2024, Номер 28(1), С. 122 - 128
Опубликована: Янв. 1, 2024
Abstract: Background: Periodontitis is a multifactorial, polymicrobial oral inflammatory illness brought on by pathogens. Porphyromonas gingivalis Gram-negative, obligatory anaerobic, black-pigmented coccobacillus and regarded as primary etiological factor in the progression of periodontitis. Rapid, highly senstitive specific detection methods are emerging. The present study aimed to evaluate loop-mediated isothermal amplification (LAMP) technique for efficiently detecting P . from subgingival plaque samples chronic periodontitis patients. Materials Methods: This included 50 patients suffering DNA (Deoxyribonucleic acid) was extracted “modified proteinase K” method. A set six primers, targeting pepO gene , used conducting LAMP. visualized naked-eye agarose electrophoresis. Conventional polymerase chain reaction (PCR) real-time qantitative PCR (qPCR) were carried out 16SrRNA (16S ribosomal ribonucleic Results: results showed that LAMP detected 40 (80%). Whereas, qPCR conventional P. 38 (76%) 33 (66%) respectively. sensitivity specificity method 94.87% 90.90%, With qPCR, found be 92.30% 81.81%, respectively, whereas, with PCR, it 76.92% 72.72%, Conclusion: an efficient quick, accurate, reliable identification samples. needs validated analytically, further studies can conducted taking saliva and/or gingival crevicular fluid
Язык: Английский
Процитировано
0