Evaluation of loop-mediated isothermal amplification method for efficient detection of the periodontopathic bacteria Porphyromonas gingivalis DOI Creative Commons

Meenaz N. Sangolli,

Manohar S. Kugaji, Suman Kumar Ray

et al.

Journal of Indian Society of Periodontology, Journal Year: 2024, Volume and Issue: 28(1), P. 122 - 128

Published: Jan. 1, 2024

Abstract: Background: Periodontitis is a multifactorial, polymicrobial oral inflammatory illness brought on by pathogens. Porphyromonas gingivalis Gram-negative, obligatory anaerobic, black-pigmented coccobacillus and regarded as primary etiological factor in the progression of periodontitis. Rapid, highly senstitive specific detection methods are emerging. The present study aimed to evaluate loop-mediated isothermal amplification (LAMP) technique for efficiently detecting P . from subgingival plaque samples chronic periodontitis patients. Materials Methods: This included 50 patients suffering DNA (Deoxyribonucleic acid) was extracted “modified proteinase K” method. A set six primers, targeting pepO gene , used conducting LAMP. visualized naked-eye agarose electrophoresis. Conventional polymerase chain reaction (PCR) real-time qantitative PCR (qPCR) were carried out 16SrRNA (16S ribosomal ribonucleic Results: results showed that LAMP detected 40 (80%). Whereas, qPCR conventional P. 38 (76%) 33 (66%) respectively. sensitivity specificity method 94.87% 90.90%, With qPCR, found be 92.30% 81.81%, respectively, whereas, with PCR, it 76.92% 72.72%, Conclusion: an efficient quick, accurate, reliable identification samples. needs validated analytically, further studies can conducted taking saliva and/or gingival crevicular fluid

Language: Английский

Ultrasensitive and fast detection of SARS-CoV-2 using RT-LAMP without pH-dependent dye DOI

Moneerah Alsaeed,

Galyah Alhamid, Hüseyin Tombuloğlu

et al.

Functional & Integrative Genomics, Journal Year: 2024, Volume and Issue: 24(1)

Published: Jan. 20, 2024

Language: Английский

Citations

6
Establishment of triple-RPA-LFS detection method for three common shrimp viruses DOI

Qing Mu,

Cun-Bao Ding, Ying Xie

et al.

Journal of Virological Methods, Journal Year: 2025, Volume and Issue: unknown, P. 115156 - 115156

Published: April 1, 2025

Language: Английский

Citations

0
Self-Diagnosis of SARS-CoV-2 from Saliva Samples at Home: Isothermal Amplification Enabled by Do-It-Yourself Portable Incubators and Laminated Poly-ethyl Sulfonate Membranes DOI Creative Commons
Sergio Bravo-González, Everardo Gonzalez‐González, Valeria Perales-Salinas

et al.

Diagnostics, Journal Year: 2024, Volume and Issue: 14(2), P. 221 - 221

Published: Jan. 19, 2024

COVID-19 made explicit the need for rethinking way in which we conduct testing epidemic emergencies. During pandemic, dependence on centralized lab facilities and resource-intensive methodologies (e.g., RT-qPCR methods) greatly limited deployment of widespread efforts many developed underdeveloped countries. Here, illustrate development a simple portable diagnostic kit that enables self-diagnosis at home from saliva samples. We describe do-it-yourself (DIY) incubator Eppendorf tubes can be used to SARS-CoV-2 detection with competitive sensitivity selectivity home. In proof-of-concept experiment, assembled Eppendorf-tube incubators our shop, prepared single-tube mix reagents LAMP primers lab, deployed these kits using urban delivery systems (i.e., Rappifavor or Uber) more than 15 different locations Monterrey, México. This straightforward strategy enabled rapid cost-effective at-home molecular diagnostics real samples high (100%) (87%).

Language: Английский

Citations

2
LAMP based electrochemical sensor for extraction-free detection of Listeria monocytogenes in food samples DOI
Ane Rivas-Macho, Unai Eletxigerra, Ruth Diez‐Ahedo

et al.

Food Control, Journal Year: 2024, Volume and Issue: 163, P. 110546 - 110546

Published: April 26, 2024

Language: Английский

Citations

2
An automated syringe-based PoC RT-LAMP LFB platform for infectious disease detection from saliva DOI Creative Commons
Dulguunnaran Naranbat,

John Murphy,

Anubhav Tripathi

et al.

Frontiers in Lab on a Chip Technologies, Journal Year: 2024, Volume and Issue: 3

Published: Aug. 27, 2024

Decentralized Point-of-Care (PoC) diagnostics hold momentous potential for rapid and accessible viral infection disease detection. Presented is a unique design application of an easy-to-use (plug-and-play) platform The leverages simplified multiplex Reverse-Transcription Loop-mediated Isothermal Amplification (RT-LAMP) Lateral Flow Biosensor (LFB) assay with lyophilized master mix, eliminating the need RNA isolation or special reporting equipment. A user-friendly Saliva Measuring Tube (SMT) ensures accurate saliva volume self-collection, Syringe-based PoC (SPoC) automates sample treatment, reagent mixing, temperature control using readily available components consumables. platform’s performance was evaluated by multiplexed detection SARS-CoV-2 N2 target gene human ACTB from samples. SPoC achieved limit spiked 500 copies/mL consistent internal readout. presented system offers promising initial step further development toward decentralized solution testing.

Language: Английский

Citations

2
Development and validation of RT-LAMP for detecting yellow fever virus in non-human primates samples from Brazil DOI Creative Commons
Sabrina Fernandes Cardoso,

Andre Akira Gonzaga Yoshikawa,

Iara Carolini Pinheiro

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Sept. 28, 2024

Language: Английский

Citations

1
Clinical Performance of the Reverse Transcription-Loop-Mediated Isothermal Amplification Assay for the Diagnosis of COVID-19 in a Thai Community Hospital at the Thailand-Myanmar Border DOI Open Access

Thanee Wongchai,

Nuttagarn Chuenchom,

Wiphat Klayut

et al.

Cureus, Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 19, 2024

Coronavirus disease 2019 (COVID-19) continues to be a global health threat and is public issue in Thailand other countries. The extensive cross-border between Myanmar considered at potentially high risk for COVID-19 distribution this region. In instance, simple cost-effective tests rapid early detection of would useful effective patient management control the disease.

Language: Английский

Citations

0
Comparative Multi-Country Evaluation of Colorimetric Rt-Lamp for Sars-Cov-2 Detection from Nasopharyngeal Swabs or from Crude Saliva in Africa DOI

Urša Šušnjar,

Molalegne Bitew,

Samuele Ayele

et al.

Published: Jan. 1, 2024

Background: Reverse-transcriptase loop-mediated isothermal amplification (RT-LAMP) is a rapid, robust, simple, and cost-effective one-step assay for the identification of SARS-CoV-2 RNA in nasopharyngeal (NP) swabs. Herein, we demonstrate expansion RT-LAMP Africa its operational performance directly from saliva as pragmatic approach outbreak surveillance response resource-limited settings Africa.Methods: A multi-country diagnostic trial was conducted between September 2021 June 2022 on 2774 NP swabs seven countries (Angola, Burkina-Faso, Ivory Coast, Ethiopia, Senegal, Sudan, Zimbabwe). In parallel, 577 samples were tested with (without extraction) four (Cameroon, Kenya, Nigeria). both contexts, real-time quantitative polymerase chain reaction (RT-qPCR) using extracted used gold-standard benchmarking to evaluate RT-LAMP.Findings: The overall sensitivity specificity across 89% (95%CI: 87-90%) 95% 93-96%), respectively, positive predictive value (PPV) 94-96%) negative (NPV) 88% 86-89%). Similarly, applied without extraction showed 80% 75-84%) sensitivity, 99% 96-100%) specificity, (95% CI: 97-100) PPV 77% 77-82) NPV.Interpretation: Colorimetric reliable detection crude samples. Its good underscores scalability efficient settings.Funding: This work supported, whole or part, by Bill & Melinda Gates Foundation [Grant No. INV-034,799].Declaration Interest: authors declare that this study supported INV-034,799]. Furthermore, New England Biolabs provided reagents free charge. have no additional interests declare.Ethical Approval: Ethical approvals obtained each country, detailed Supplementary Tables 2 3.

Language: Английский

Citations

0
High-Sensitivity RT-LAMP for Molecular Detection of O’nyong-nyong (Alphavirus onyong) DOI Creative Commons

David Faísca-Silva,

Gonçalo Seixas, Mónica Nunes

et al.

Pathogens, Journal Year: 2024, Volume and Issue: 13(10), P. 892 - 892

Published: Oct. 11, 2024

Mosquitoes serve as vectors for many arthropod-borne viruses (arboviruses) that are responsible millions of human infections and thousands deaths each year. Among these arboviruses, O'nyong-nyong virus (ONNV) is an African alphavirus mainly transmitted by

Language: Английский

Citations

0
Evaluation of a Commercial Point-of-Care RT-LAMP Assay for Rapid Detection of SARS-CoV-2 DOI Creative Commons

Janet Hei Yin Law,

Wai Sing Chan,

Tsun Leung Chan

et al.

Biomedicines, Journal Year: 2023, Volume and Issue: 11(9), P. 2344 - 2344

Published: Aug. 23, 2023

The goal of this study was to evaluate the performance a commercial reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay (Detect COVID-19 Test) in detection severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). A total 202 human and viral culture specimens were tested retrospectively. Detect Test comparable that real-time polymerase chain reaction assays (sensitivity: 93.42%; specificity: 100%), better than rapid antigen test 48.00%; 100%) for with threshold cycle (Ct) values less 30. Beta, Delta, Omicron variants concern successfully detected. With their simplicity use good sensitivity, point-of-care RT-LAMP may be viable option SARS-CoV-2 testing at home, or regions without sophisticated laboratory facilities.

Language: Английский

Citations

1