Lactobacillus Plantarum Mediated Tryptophan Metabolism to Alleviate Tropomyosin-induced Food Allergy Through the Aryl Hydrocarbon Receptor DOI Creative Commons
Jing Ma,

Pengyan Tong,

Yining Jia

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Ноя. 8, 2024

Abstract Background: Recently, food allergy (FA) has become increasingly prevalent, affecting daily life and possibly endangering lives. Probiotics ameliorate FA by modulating immune-microbial interactions. However, the effective metabolites mechanism of alleviating in Lactobacillus Plantarum (L. Plantarum) remain to be elucidated. Results: In present study, L. strains JM113, PA01, 21805 demonstrated ability improve regulatory T (Treg)/Th1/Th2 balance, reshaping gut microbial composition, regulating metabolites. Tropomyosin sensitisation was associated with a decreased levels 5-Hydroxyindole-3-acetic acid (5-HIAA) cecum contents, as evidenced serum mice patients FA. Cellular experiments revealed that tryptophan (Trp), tryptamine, tryptophol, kynurenine, 5-HIAA, indole-3-acetamide inhibited degranulation RHL-2H cells; however, these benefits were eliminated AHR antagonist CH223191. Furthermore, vivo results suggested 5-HIAA Trp diets downregulated IgE, β-Hex, HIS, IL-4, IL-13, while upregulated concentrations IFN-γ, TGF-β activate AhR-mediated immune response. Intestinal morphological analysis increased number cup cells recover intestinal damage. Conclusions: summary, beneficial effect on appear rely AhR-related pathways, providing compelling evidence for positive role

Язык: Английский

Anti-Allergic and Anti-Inflammatory Signaling Mechanisms of Natural Compounds/Extracts in In Vitro System of RBL-2H3 Cell: A Systematic Review DOI Creative Commons
Tekan S. Rana,

Rishipal R. Bansode,

Leonard Williams

и другие.

Cells, Год журнала: 2024, Номер 13(16), С. 1389 - 1389

Опубликована: Авг. 21, 2024

Various extracts are tested for anti-allergic or anti-inflammatory properties on in vitro models. RBL-2H3 cells widely used allergic immunological studies. FCεRI and its downstream signaling cascades, such as MAPK, NF-κB, JAK/STAT pathways, important inflammatory mechanisms mast basophil cells. This systematic review aims to study common pathways of the compounds We selected relevant research articles published after 2015 from PubMed, Scopus, Science Direct Web databases. The risk bias studies was assessed based modified CONSORT checklist cell lines, treatments, assay, primary findings, were extracted synthesize results. Thirty-eight included, Lyn, Sky, PLCγ, commonly studied. Moreover, pathway a potential mechanism However, findings needed be along with human confirm relevance health. In conclusion, single plant extract may act an reagent via multiple besides MAPK pathway.

Язык: Английский

Процитировано

3
Exploring the anti-anaphylaxis potential of natural products: A Review DOI Creative Commons
Ahmed Salem Eid,

Eman M. Zaki,

Manal M. Sabry

и другие.

Inflammopharmacology, Год журнала: 2025, Номер unknown

Опубликована: Март 19, 2025

Abstract Allergies are a common health issue affecting many people around the world, especially in developed countries. They occur when immune system overreacts to substances that usually harmless. Some allergic conditions include asthma, sinus infections, skin rashes, food allergies, hay fever, severe reactions, eczema, swelling, and reactions medications or insect stings. The causes of these allergies complex often linked genetics, which can lead heightened responses known as atopy. Throughout history, plant extracts have been used for various purposes, including medicine food. In addition, their bioactive compounds show wide range beneficial effects, such reducing fighting oxidative stress, mast cell stabilizers, lowering inflammation, highlighting potential treating conditions. Flavonoids phenolic commonly anaphylaxis potent anti-inflammatory action. This review aims promote use natural products treatments anaphylaxis. discovery new drugs derived from sources holds significant promise management

Язык: Английский

Процитировано

0
Sea buckthorn: A potential prebiotic and promising functional ingredient for fermented dairy products DOI Creative Commons
Jiayi Tang, Henan Zhang, Rina Wu

и другие.

Journal of Future Foods, Год журнала: 2025, Номер unknown

Опубликована: Апрель 1, 2025

Язык: Английский

Процитировано

0
Sea buckthorn-derived extracellular vesicles foster bone regeneration through aau-miR168-mediated pathways DOI Creative Commons
Mengxin Zhao, Xiaolin Chen, Wenyan Wang

и другие.

Stem Cell Research & Therapy, Год журнала: 2025, Номер 16(1)

Опубликована: Июнь 3, 2025

Plant-derived extracellular vesicles (P-EVs) possess remarkable therapeutic potential, yet the regenerative capabilities of sea buckthorn-derived (SAEVs) remain underexplored. This study aims to elucidate osteogenic and bone-healing properties SAEVs. SAEVs were isolated from buckthorn juice via differential centrifugation characterized using electron microscopy dynamic light scattering. Bone marrow mesenchymal stromal cells (BMSCs) treated with SAEVs, cellular uptake was evaluated through fluorescence flow cytometry. In vivo, DiD-labeled orally administered mice determine biodistribution IVIS imaging. A murine femoral defect model employed assess bone efficacy delivered or without GelMA hydrogels, analyzed by micro-CT histological staining. Small RNA sequencing identified SAEV-derived miRNAs, luciferase reporter assays validated miRNA-mediated regulation genes. efficiently internalized into BMSCs macropinocytosis, promoting expression key markers such as Runx2 osteocalcin. SAEV-GelMA hydrogels significantly accelerated regeneration in a inducing adverse hematological effects, affirming safety SAEV administration. Mechanistic investigations revealed an enrichment particularly aau-miR168, which modulates osteogenesis aau-miR168/LBH/RUNX2 signaling cascade. highlights transformative biocompatible strategy for fracture healing osteoporosis management, offering novel avenue medicine.

Язык: Английский

Процитировано

0
Sea Buckthorn Flavonoid Extracted with High Hydrostatic Pressure Alleviated Shrimp Allergy in Mice through the Microbiota and Metabolism DOI
Xiaoping Feng,

Zhuomin Yan,

Xiaojing Ren

и другие.

Journal of Agricultural and Food Chemistry, Год журнала: 2024, Номер 72(45), С. 25094 - 25102

Опубликована: Ноя. 4, 2024

Sea buckthorn (Hippophaë rhamnoides L.) known as the deciduous shrub has been reported to have effects of antioxidant, anti-inflammatory, and immunomodulatory activities. Tropomyosin (TM) induced a regulatory immune response associated with food allergy. In this study, mouse model allergy sensitized tropomyosin was established assess antiallergic properties sea flavonoid extract (SBF). SBF alleviated mice's allergic symptoms exhibited significant reduction in levels IgE histamine. Meanwhile, repaired Th2 cell overpolarization generated by TM, via downregulating IL-4 production upregulating IFN-γ restore balance Th1/Th2 cells. Furthermore, 16S RNA analysis showed that primarily restored gut microbiota increasing abundance Chitinophilidae decreasing Burkholderiaceae, Pneumatobacteriaceae, Sphingomonadaceae. Gut metabolomes determined liquid chromatography–mass spectrometry (LC–MS) suggested TM upregulated PE (14:0/22:1(13Z)) decreased formimino-l-glutamic acid urocanic levels. According KEGG pathway analysis, treatment shown modulate glycerophospholipid histidine metabolism improve reactions. holds great promise novel potential agent for allergies.

Язык: Английский

Процитировано

2
Lactobacillus Plantarum Mediated Tryptophan Metabolism to Alleviate Tropomyosin-induced Food Allergy Through the Aryl Hydrocarbon Receptor DOI Creative Commons
Jing Ma,

Pengyan Tong,

Yining Jia

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Ноя. 8, 2024

Abstract Background: Recently, food allergy (FA) has become increasingly prevalent, affecting daily life and possibly endangering lives. Probiotics ameliorate FA by modulating immune-microbial interactions. However, the effective metabolites mechanism of alleviating in Lactobacillus Plantarum (L. Plantarum) remain to be elucidated. Results: In present study, L. strains JM113, PA01, 21805 demonstrated ability improve regulatory T (Treg)/Th1/Th2 balance, reshaping gut microbial composition, regulating metabolites. Tropomyosin sensitisation was associated with a decreased levels 5-Hydroxyindole-3-acetic acid (5-HIAA) cecum contents, as evidenced serum mice patients FA. Cellular experiments revealed that tryptophan (Trp), tryptamine, tryptophol, kynurenine, 5-HIAA, indole-3-acetamide inhibited degranulation RHL-2H cells; however, these benefits were eliminated AHR antagonist CH223191. Furthermore, vivo results suggested 5-HIAA Trp diets downregulated IgE, β-Hex, HIS, IL-4, IL-13, while upregulated concentrations IFN-γ, TGF-β activate AhR-mediated immune response. Intestinal morphological analysis increased number cup cells recover intestinal damage. Conclusions: summary, beneficial effect on appear rely AhR-related pathways, providing compelling evidence for positive role

Язык: Английский

Процитировано

0