The Science of The Total Environment,
Год журнала:
2024,
Номер
957, С. 177834 - 177834
Опубликована: Ноя. 30, 2024
The
COVID-19
pandemic
has
underscored
the
need
for
effective
viral
tracking
in
aqueous
environments,
particularly
non-enteric
viruses.
Despite
advances
wastewater
monitoring,
surveillance
of
viruses
freshwater
remains
limited
due
to
traditional
sampling
challenges.
This
study
refines
GAC-based
passive
protocols
by
determining
optimal
extraction
and
elution
methods
enhancing
recovery
nucleic
acids
freshwater.
Three
commercially
available
total
acid
(TNA)
kits
four
buffers
were
assessed
their
ability
recover
SARS-CoV-2
bacteriophage
MS2
from
samplers.
Promega
Wizard®
Enviro
Total
Nucleic
Acid
Kit,
paired
with
a
Tween®20-based
buffer,
provided
highest
virus
efficiency
sampling.
Field-scale
applications
demonstrated
effectiveness
samplers
capturing
SARS-CoV-2,
INFA,
RSV,
MeV
using
optimized
protocols.
combination
Tween®20
based
buffer
kit
led
increased
detection
frequencies
grab
samples,
which
remained
lower
than
underscores
importance
selecting
appropriate
TNA
maximize
samples.
By
optimizing
these
protocols,
we
enhance
sensitivity
reliability
ecosystems.
Water Research,
Год журнала:
2024,
Номер
261, С. 122024 - 122024
Опубликована: Июнь 30, 2024
Water
quality,
critical
for
human
survival
and
well-being,
necessitates
rigorous
control
to
mitigate
contamination
risks,
particularly
from
pathogens
amid
expanding
urbanization.
Consequently,
the
necessity
maintain
microbiological
safety
of
water
supplies
demands
effective
surveillance
strategies,
reliant
on
collection
representative
samples
precise
measurement
contaminants.
This
review
critically
examines
advancements
passive
sampling
techniques
monitoring
in
various
systems,
including
wastewater,
freshwater,
seawater.
We
explore
evolution
conventional
materials
innovative
adsorbents
pathogen
capture
shift
culture-based
molecular
detection
methods,
underscoring
adaptation
this
field
global
health
challenges.
The
comparison
highlights
sampling's
efficacy
over
like
grab
its
potential
overcome
existing
challenges
through
use
such
as
granular
activated
carbon,
thermoplastics,
polymer
membranes.
By
evaluating
literature,
work
identifies
standardization
gaps
proposes
future
research
directions
augment
efficiency,
specificity,
utility
environmental
public
surveillance.
Passive
sampling
has
shown
promise
as
an
alternative
approach
for
monitoring
of
pathogens
in
aquatic
matrices.
We
conducted
two
controlled
experiments
to
compare
the
efficacy
membrane
passive
composite
both
wastewater
and
surface
water
detection
Escherichia
coli
Cryptosporidium.
also
investigated
relative
uptake
E.
Cryptosporidium
onto
samplers
over
time.
Both
methods
returned
positive
detections
at
all
deployment
times
(4,
8,
24,
48,
72,
96
h)
showed
similar
rates
samples
(31%
passive;
41%
composite)
(76%
86%
composite).
found
significant
linear
up
h
(R2
=
0.932;
p
0.002).
In
wastewater,
maximum
sampler
was
reached
after
24
h.
For
Cryptosporidium,
observed
0.805;
0.015)
0.877;
0.006).
Our
results
support
that
may
be
used
waters
Abstract
Background
Human
viruses
released
into
the
environment
can
be
detected
and
characterized
in
wastewater.
The
study
of
wastewater
virome
offers
a
consolidated
perspective
on
circulation
within
population.
Because
occurrence
severity
viral
infections
vary
across
person’s
lifetime,
studying
samples
contributed
by
various
demographic
segments
provide
valuable
insights
prevalence
these
segments.
In
our
study,
targeted
enrichment
sequencing
was
employed
to
characterize
human
at
building-level
scale.
This
accomplished
through
passive
sampling
schools,
university
settings,
nursing
homes
two
cities
Catalonia.
Additionally,
sewage
from
large
urban
treatment
plant
analysed
serve
as
reference
for
examining
collective
excreted
virome.
Results
obtained
influent
showcased
combined
presence
individuals
varying
ages,
with
astroviruses
bocaviruses
being
most
prevalent,
followed
adenoviruses,
polyomaviruses,
papillomaviruses.
Significant
variations
profiles
were
observed
among
different
types
buildings
studied.
Mamastrovirus
1
predominant
school
samples,
salivirus
polyomaviruses
JC
BK
settings
while
showed
more
balanced
distribution
families
presenting
papillomavirus
picornaviruses
and,
interestingly,
some
linked
immunosuppression.
Conclusions
shows
utility
wastewater-based
epidemiology
an
effective
tool
monitoring
circulating
specific
age
groups.
It
provides
public
health
epidemiological
studies.
The Science of The Total Environment,
Год журнала:
2023,
Номер
887, С. 164143 - 164143
Опубликована: Май 13, 2023
Wastewater-based
monitoring
of
SARS-CoV-2
has
become
a
promising
and
useful
tool
in
tracking
the
potential
spread
or
dynamics
virus.
Its
recording
can
be
used
to
predict
how
number
infections
population
will
develop.
Recent
studies
have
shown
that
use
passive
samplers
is
also
suitable
for
detection
genome
copies
(GC)
wastewater.
They
at
any
site,
provide
timely
data
may
collect
GC
missed
by
traditional
sampling
methods.
Therefore,
aim
this
study
was
evaluate
suitability
wastewater
long-term
two
different
scales.
Polyethylene-based
plastic
were
deployed
city-scale
level
Leipzig
13
locations,
with
samples
being
taken
from
March
2021
August
2022.
At
smaller
city
district
level,
three
types
(cotton-cloth,
unravelled
polypropylene
rope
polyethylene-based
strips)
sampled
on
weekly
basis
The
results
are
discussed
relation
individual
point.
Our
show
indicate
an
accurate
positive
(positive-rate:
86
%).
On
small-scale
feasible
effective
detect
easily
cost-effectively,
mirroring
similar
trend
level.
Thus,
demonstrated
reproducible
signals
time-integrated
measurement
matrix
greater
sensitivity
compared
We
thus
recommend
as
alternative
method
wastewater-based
epidemiology.
Passive
particular
considered
better
estimation
incidence
levels.
Concentrations
of
pathogen
genomes
measured
in
wastewater
have
recently
become
available
as
a
new
data
source
to
use
when
modeling
the
spread
infectious
diseases.
One
promising
for
this
is
inference
effective
reproduction
number,
average
number
individuals
newly
infected
person
will
infect.
We
propose
model
where
infections
arrive
according
time-varying
immigration
rate
which
can
be
interpreted
compound
parameter
equal
product
proportion
susceptibles
population
and
transmission
rate.
This
allows
us
estimate
from
concentrations
while
avoiding
difficult
verify
assumptions
about
dynamics
susceptible
population.
As
byproduct
our
primary
goal,
we
also
produce
estimating
case
using
same
framework.
test
framework
an
agent-based
simulation
study
with
realistic
generating
mechanism
accounts
shedding.
Finally,
apply
SARS-CoV-2
Los
Angeles,
California,
RNA
collected
large
treatment
facility.
Water Research,
Год журнала:
2024,
Номер
266, С. 122284 - 122284
Опубликована: Авг. 22, 2024
Traditional
methods
for
monitoring
pathogens
in
environmental
waters
have
numerous
drawbacks.
Sampling
approaches
that
are
low-cost
and
time
efficient
can
capture
temporal
variation
microbial
contamination
needed.
Passive
sampling
of
aquatic
environments
has
shown
promise
as
an
alternative
water
technique
waterborne
contaminants.
The
present
systematic
review
aimed
to
compile
synthesize
existing
literature
on
the
use
passive
samplers
microbes
different
sources
identify
research
gaps.
summarizes
current
knowledge
materials
used
detection,
deployment
durations,
analytical
methods,
quantification
well
benefits
limitations
sampling.
This
found
electronegative
nitrocellulose
membrane
filters
effective
both
detection
viruses
wastewater,
while
gauze
been
detecting
bacterial
targets
wastewater.
There
is
a
large
gap
quantitative
manner,
especially
back-calculation
water-column
concentrations
or
correlation
outcomes
interest
(e.g.
prevalence
rates).
Further,
there
very
limited
attention
paid
bacteria
any
source
lack
studies
utilizing
protozoa.
