From capture to detection: A critical review of passive sampling techniques for pathogen surveillance in water and wastewater

Water Research, Journal Year: 2024, Volume and Issue: 261, P. 122024 - 122024

Published: June 30, 2024

Water quality, critical for human survival and well-being, necessitates rigorous control to mitigate contamination risks, particularly from pathogens amid expanding urbanization. Consequently, the necessity maintain microbiological safety of water supplies demands effective surveillance strategies, reliant on collection representative samples precise measurement contaminants. This review critically examines advancements passive sampling techniques monitoring in various systems, including wastewater, freshwater, seawater. We explore evolution conventional materials innovative adsorbents pathogen capture shift culture-based molecular detection methods, underscoring adaptation this field global health challenges. The comparison highlights sampling's efficacy over like grab its potential overcome existing challenges through use such as granular activated carbon, thermoplastics, polymer membranes. By evaluating literature, work identifies standardization gaps proposes future research directions augment efficiency, specificity, utility environmental public surveillance.

Language: Английский

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Wastewater-based epidemiology applied at the building-level reveals distinct virome profiles based on the age of the contributing individuals

Human Genomics, Journal Year: 2024, Volume and Issue: 18(1)

Published: Feb. 1, 2024

Abstract Background Human viruses released into the environment can be detected and characterized in wastewater. The study of wastewater virome offers a consolidated perspective on circulation within population. Because occurrence severity viral infections vary across person’s lifetime, studying samples contributed by various demographic segments provide valuable insights prevalence these segments. In our study, targeted enrichment sequencing was employed to characterize human at building-level scale. This accomplished through passive sampling schools, university settings, nursing homes two cities Catalonia. Additionally, sewage from large urban treatment plant analysed serve as reference for examining collective excreted virome. Results obtained influent showcased combined presence individuals varying ages, with astroviruses bocaviruses being most prevalent, followed adenoviruses, polyomaviruses, papillomaviruses. Significant variations profiles were observed among different types buildings studied. Mamastrovirus 1 predominant school samples, salivirus polyomaviruses JC BK settings while showed more balanced distribution families presenting papillomavirus picornaviruses and, interestingly, some linked immunosuppression. Conclusions shows utility wastewater-based epidemiology an effective tool monitoring circulating specific age groups. It provides public health epidemiological studies.

Language: Английский

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Parallel deployment of passive and composite samplers for surveillance and variant profiling of SARS-CoV-2 in sewage

The Science of The Total Environment, Journal Year: 2022, Volume and Issue: 866, P. 161101 - 161101

Published: Dec. 27, 2022

Language: Английский

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A long-term passive sampling approach for wastewater-based monitoring of SARS-CoV-2 in Leipzig, Germany

The Science of The Total Environment, Journal Year: 2023, Volume and Issue: 887, P. 164143 - 164143

Published: May 13, 2023

Wastewater-based monitoring of SARS-CoV-2 has become a promising and useful tool in tracking the potential spread or dynamics virus. Its recording can be used to predict how number infections population will develop. Recent studies have shown that use passive samplers is also suitable for detection genome copies (GC) wastewater. They at any site, provide timely data may collect GC missed by traditional sampling methods. Therefore, aim this study was evaluate suitability wastewater long-term two different scales. Polyethylene-based plastic were deployed city-scale level Leipzig 13 locations, with samples being taken from March 2021 August 2022. At smaller city district level, three types (cotton-cloth, unravelled polypropylene rope polyethylene-based strips) sampled on weekly basis The results are discussed relation individual point. Our show indicate an accurate positive (positive-rate: 86 %). On small-scale feasible effective detect easily cost-effectively, mirroring similar trend level. Thus, demonstrated reproducible signals time-integrated measurement matrix greater sensitivity compared We thus recommend as alternative method wastewater-based epidemiology. Passive particular considered better estimation incidence levels.

Language: Английский

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Passive swab versus grab sampling for detection of SARS-CoV-2 markers in wastewater

The Science of The Total Environment, Journal Year: 2023, Volume and Issue: 889, P. 164180 - 164180

Published: May 16, 2023

Language: Английский

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Semiparametric inference of effective reproduction number dynamics from wastewater pathogen surveillance data

Biometrics, Journal Year: 2024, Volume and Issue: 80(3)

Published: July 1, 2024

Concentrations of pathogen genomes measured in wastewater have recently become available as a new data source to use when modeling the spread infectious diseases. One promising for this is inference effective reproduction number, average number individuals newly infected person will infect. We propose model where infections arrive according time-varying immigration rate which can be interpreted compound parameter equal product proportion susceptibles population and transmission rate. This allows us estimate from concentrations while avoiding difficult verify assumptions about dynamics susceptible population. As byproduct our primary goal, we also produce estimating case using same framework. test framework an agent-based simulation study with realistic generating mechanism accounts shedding. Finally, apply SARS-CoV-2 Los Angeles, California, RNA collected large treatment facility.

Language: Английский

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Assessing Passive Sampling for the Monitoring of E. coli and Cryptosporidium spp. in Environmental Waters

ACS ES&T Water, Journal Year: 2025, Volume and Issue: unknown

Published: March 25, 2025

Passive sampling has shown promise as an alternative approach for monitoring of pathogens in aquatic matrices. We conducted two controlled experiments to compare the efficacy membrane passive composite both wastewater and surface water detection Escherichia coli Cryptosporidium. also investigated relative uptake E. Cryptosporidium onto samplers over time. Both methods returned positive detections at all deployment times (4, 8, 24, 48, 72, 96 h) showed similar rates samples (31% passive; 41% composite) (76% 86% composite). found significant linear up h (R2 = 0.932; p 0.002). In wastewater, maximum sampler was reached after 24 h. For Cryptosporidium, observed 0.805; 0.015) 0.877; 0.006). Our results support that may be used waters

Language: Английский

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Monitoring COVID-19 spread in selected Prague's schools based on the presence of SARS-CoV-2 RNA in wastewater

The Science of The Total Environment, Journal Year: 2023, Volume and Issue: 871, P. 161935 - 161935

Published: Jan. 30, 2023

Language: Английский

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Evaluation of wastewater surveillance for SARS-CoV-2 in Massachusetts correctional facilities, 2020–2022

Frontiers in Water, Journal Year: 2023, Volume and Issue: 5

Published: Jan. 26, 2023

Introduction Correctional facilities have environmental, resource, and organizational factors that facilitate SARS-CoV-2 transmission challenge clinical testing of staff residents. In Massachusetts, multiple state prisons implemented wastewater surveillance for strategic individuals isolation COVID-19 cases early in the course infection, as recommended by Centers Disease Control Prevention (CDC). Our objective was to quantify correlation with facility-level compared standard case towns closest geographic proximity participating correctional facilities. Materials methods Available data included number reported residents from each eight (labeled A-H anonymity), viral concentrations at facility, routine geographically nearest facility. We selected December 2020-February 2022. Spearman's rank calculated facility assess agreement between town resident cases, cases. considered a ≤0.3 weak ≥0.6 strong. Results Facilities housed mean 502 (range 54–1,184) staffing 341 53–547). 7/8 facilities, town/resident coefficients (ρ) were statistically significant 0.22–0.65); all wastewater/facility correlations 0.57–0.82). Consistently, ρ values higher facility-specific wastewater/resident than cases: A (0.65, 0.80), B (0.59, 0.81), C (0.55, 0.70), D (0.61, 0.82), E (0.46, 0.62), F (0.51, H (0.22, 0.57). Conclusion conclude can provide an additional signal objectively supplement existing detection infection control efforts

Language: Английский

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Passive sampling of microbes in various water sources: A systematic review

Water Research, Journal Year: 2024, Volume and Issue: 266, P. 122284 - 122284

Published: Aug. 22, 2024

Traditional methods for monitoring pathogens in environmental waters have numerous drawbacks. Sampling approaches that are low-cost and time efficient can capture temporal variation microbial contamination needed. Passive sampling of aquatic environments has shown promise as an alternative water technique waterborne contaminants. The present systematic review aimed to compile synthesize existing literature on the use passive samplers microbes different sources identify research gaps. summarizes current knowledge materials used detection, deployment durations, analytical methods, quantification well benefits limitations sampling. This found electronegative nitrocellulose membrane filters effective both detection viruses wastewater, while gauze been detecting bacterial targets wastewater. There is a large gap quantitative manner, especially back-calculation water-column concentrations or correlation outcomes interest (e.g. prevalence rates). Further, there very limited attention paid bacteria any source lack studies utilizing protozoa.

Language: Английский

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