Frontiers in Genetics,
Год журнала:
2022,
Номер
13
Опубликована: Авг. 4, 2022
Legume
crops
provide
significant
nutrition
to
humans
as
a
source
of
protein,
omega-3
fatty
acids
well
specific
macro
and
micronutrients.
Additionally,
legumes
improve
the
cropping
environment
by
replenishing
soil
nitrogen
content.
Chickpeas
are
second
most
staple
legume
food
crop
worldwide
behind
dry
bean
which
contains
17%-24%
41%-51%
carbohydrate,
other
important
essential
minerals,
vitamins,
dietary
fiber,
folate,
β-carotene,
anti-oxidants,
micronutrients
(phosphorus,
calcium,
magnesium,
iron,
zinc)
linoleic
oleic
unsaturated
acids.
Despite
these
advantages,
far
cereals
in
terms
genetic
improvement
mainly
due
less
effort,
bottlenecks
narrow
base,
several
biotic
abiotic
factors
scenario
changing
climatic
conditions.
Measures
now
called
for
beyond
conventional
breeding
practices
strategically
broadening
base
utilizing
chickpea
wild
relatives
cultivars
through
advanced
approaches
with
focus
on
high
yield
productivity,
stresses
including
climate
resilience,
enhanced
nutritional
values.
Desirable
donors
having
such
multiple
traits
have
been
identified
using
core
mini
collections
from
cultivated
gene
pool
Chickpea.
Several
methods
developed
address
cross-species
fertilization
obstacles
aid
inter-specific
hybridization
introgression
target
sequences
Cicer
species.
recent
advances
"Omics"
sciences
along
high-throughput
precise
phenotyping
tools
made
it
easier
identify
genes
that
regulate
interest.
Next-generation
sequencing
technologies,
whole-genome
sequencing,
transcriptomics,
differential
expression
profiling
plethora
novel
techniques
like
single
nucleotide
polymorphism
exploiting
high-density
genotyping
assays,
simple
sequence
repeat
markers,
diversity
array
technology
platform,
re-sequencing
technique
led
identification
development
QTLs
trait
mapping
global
germplasm.
These
altogether
helped
chickpeas.
Frontiers in Genetics,
Год журнала:
2022,
Номер
13
Опубликована: Июнь 28, 2022
Legumes
are
rich
in
protein
and
phytochemicals
have
provided
a
healthy
diet
for
human
beings
thousands
of
years.
In
recognition
the
important
role
they
play
nutrition
agricultural
production,
researchers
made
great
efforts
to
gain
new
genetic
traits
legumes
such
as
yield,
stress
tolerance,
nutritional
quality.
recent
years,
significant
increase
genomic
resources
legume
plants
has
prepared
groundwork
applying
cutting-edge
breeding
technologies,
transgenic
genome
editing,
selection
crop
improvement.
addition
different
editing
technologies
including
CRISPR/Cas9-based
system,
this
review
article
discusses
advances
plant-specific
gene-editing
methods,
well
problems
potential
benefits
associated
with
improvement
crops
agronomic
properties.
The
been
effectively
used
model
like
alfalfa
lotus,
soybean,
cowpea,
chickpea.
We
also
discussed
methods
improvements
recalcitrant
legumes.
Despite
immense
opportunities
can
offer
legumes,
governmental
regulatory
restrictions
present
major
concern.
context,
comparison
framework
strategies
European
Union
United
States
America
was
discussed.
Gene-editing
opened
up
possibilities
breeding.
International Journal of Molecular Sciences,
Год журнала:
2022,
Номер
23(17), С. 9809 - 9809
Опубликована: Авг. 29, 2022
Precise
editing
of
the
plant
genome
has
long
been
desired
for
functional
genomic
research
and
crop
breeding.
Prime
is
a
newly
developed
precise
technology
based
on
CRISPR-Cas9,
which
uses
an
engineered
reverse
transcriptase
(RT),
catalytically
impaired
Cas9
endonuclease
(nCas9),
prime
guide
RNA
(pegRNA).
In
addition,
wider
range
types
than
base
can
produce
nearly
all
edits.
Although
was
first
established
in
human
cells,
it
recently
applied
to
plants.
As
relatively
new
technique,
optimization
will
be
needed
increase
efficiency
different
crops.
this
study,
we
successfully
edited
mutant
GFP
rice,
peanut,
chickpea,
cowpea
protoplasts.
up
16
times
higher
achieved
with
dual
pegRNA
single
containing
vectors.
Edited-mutant
protoplasts
have
also
obtained
after
transformation
vectors,
albeit
much
lower
ranging
from
0.2%
0.5%.
These
initial
results
promise
expedite
application
legume
breeding
programs
accelerate
improvement.
Frontiers in Genetics,
Год журнала:
2022,
Номер
13
Опубликована: Авг. 4, 2022
Legume
crops
provide
significant
nutrition
to
humans
as
a
source
of
protein,
omega-3
fatty
acids
well
specific
macro
and
micronutrients.
Additionally,
legumes
improve
the
cropping
environment
by
replenishing
soil
nitrogen
content.
Chickpeas
are
second
most
staple
legume
food
crop
worldwide
behind
dry
bean
which
contains
17%-24%
41%-51%
carbohydrate,
other
important
essential
minerals,
vitamins,
dietary
fiber,
folate,
β-carotene,
anti-oxidants,
micronutrients
(phosphorus,
calcium,
magnesium,
iron,
zinc)
linoleic
oleic
unsaturated
acids.
Despite
these
advantages,
far
cereals
in
terms
genetic
improvement
mainly
due
less
effort,
bottlenecks
narrow
base,
several
biotic
abiotic
factors
scenario
changing
climatic
conditions.
Measures
now
called
for
beyond
conventional
breeding
practices
strategically
broadening
base
utilizing
chickpea
wild
relatives
cultivars
through
advanced
approaches
with
focus
on
high
yield
productivity,
stresses
including
climate
resilience,
enhanced
nutritional
values.
Desirable
donors
having
such
multiple
traits
have
been
identified
using
core
mini
collections
from
cultivated
gene
pool
Chickpea.
Several
methods
developed
address
cross-species
fertilization
obstacles
aid
inter-specific
hybridization
introgression
target
sequences
Cicer
species.
recent
advances
"Omics"
sciences
along
high-throughput
precise
phenotyping
tools
made
it
easier
identify
genes
that
regulate
interest.
Next-generation
sequencing
technologies,
whole-genome
sequencing,
transcriptomics,
differential
expression
profiling
plethora
novel
techniques
like
single
nucleotide
polymorphism
exploiting
high-density
genotyping
assays,
simple
sequence
repeat
markers,
diversity
array
technology
platform,
re-sequencing
technique
led
identification
development
QTLs
trait
mapping
global
germplasm.
These
altogether
helped
chickpeas.
