Ligustroside derived from Ligustrum japonicum inhibits melanogenesis via blocking the MAPK and PKA/CREB signaling pathways

Asian Pacific Journal of Tropical Biomedicine, Год журнала: 2025, Номер unknown

Опубликована: Янв. 17, 2025

Objective: To investigate the anti-melanogenic potential of ligustroside isolated from Ligustrum japonicum. Methods: The cytotoxicity was tested via MTT assay. Furthermore, effects on expression critical melanogenic markers such as tyrosinase, tyrosinase related proteins (TRPs), and microphthalmia-associated transcription factor (MITF) were analyzed at both mRNA protein levels RT-qPCR Western blot, respectively, in α-melanocyte stimulating hormone- induced B16F10 cells. In addition, phosphorylation p38, ERK JNK investigated. Immunofluorescence analysis MITF also conducted. Results: Ligustroside significantly reduced intracellular activity melanin content by 37.11% 29.12%, compared to untreated Moreover, it downregulated MITF, TRP-1, TRP-2 regulating mitogen-activated kinase (MAPK) A (PKA)/cAMP response element-binding (CREB) signaling pathways. suppressed nuclear β-catenin, p-CREB, decreased immunofluorescence intensity MITF. Conclusions: derived japonicum shows a significant anti-melanogenesis effect suppression MAPK PKA/CREB

Язык: Английский

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Genome-based analysis of the genetic pattern of black sheep in Qira sheep

BMC Genomics, Год журнала: 2025, Номер 26(1)

Опубликована: Фев. 6, 2025

Black wool can effectively prevent sheep from DNA damage as well fungal infection, and improve reproductive performance. In order to explore the candidate genes related black formation in Qira sheep. We selected 123 adult healthy ewes with different coat colors groups (black (B), brown (Y) grey (G)) extracted their venous blood obtain Illumina Ovine SNP 50K chip data. Subsequently, our PCA, NJ-tree, Admixture population structure analyses of 3 color populations showed that middle exhibited same genetic traits. Fst, xp-EHH, iHS, π were detected for selection signals, 5% SNPs loci positively annotated based on SheepOar_v4.0. The region exon 1 TYRP1 gene was further screened, amplified sequenced through associated goodness-of-fit using chi-square test. results 71 traits, among which TYRP1, PARD3 CDH2 strongly production. Three mutations significantly variation inQira (2:81,183,168, 2:81,183,281 2:81,183,284). this paper, could not differentiate by color, obtained wool. Detection mutation sites affecting hair change provides a basis line breed conservation.

Язык: Английский

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The study of miRNA-211 affects melanogenesis progress in Cashmere goats via suppressing AP1S2

Archives animal breeding/Archiv für Tierzucht, Год журнала: 2025, Номер 68(1), С. 125 - 134

Опубликована: Фев. 11, 2025

Abstract. Chromaticity is a key indicator of measuring economic worth Cashmere goats, and gene regulation plays major role in goat color. By targeting the 3′UTR region genes, such as miRNA-211, miRNA significant regulatory melanogenesis. But mechanism how miRNA-211 affects melanogenesis goats unclear. As result, insights into color by its brief are offered this study. First, target AP1S2 was screened TargetScan GO/KEGG, then targeted relationship confirmed dual-luciferase reporter experiment. In B16-F10 cells, which overexpress skin, opposite expression trends between were analyzed reverse transcription–quantitative PCR (RT-qPCR) western blot. And their cells immunofluorescence. The information above showed downregulated AP1S2. Afterwards, impact on animal regression studies. Utilizing immunohistochemistry, mouse hair follicle observed. Following subcutaneous injection antagomiRNA-211 (chemical modification inhibitor), levels protein enhanced, localization also increased. Furthermore, reduction melanin content skin detected. These results that significantly affected progress via downregulating advantageously skin.

Язык: Английский

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In Vitro and In Silico Studies of Maculosin as a Melanogenesis and Tyrosinase Inhibitor

Molecules, Год журнала: 2025, Номер 30(4), С. 860 - 860

Опубликована: Фев. 13, 2025

The investigation of melanogenesis and tyrosinase inhibitors is essential for developing safe effective natural compounds to treat pigmentation disorders. This study aimed evaluate the effects maculosin, a cyclic dipeptide composed tyrosine proline, on melanin production activity using B16F10 melanoma cell model, while elucidating its mechanism action through molecular docking dynamics (MD) simulations. Experimental results demonstrated that maculosin inhibited intracellular content in concentration-dependent manner cells. Molecular analyses revealed exhibited high binding affinities with mushroom (mTYR), tyrosinase-related protein 1 (TYRP1), Bacillus megaterium (BmTYR) energies -7.7, -6.8, -7.5 kcal/mol, respectively. Furthermore, MD simulations confirmed structural stability dynamic flexibility maculosin-protein complexes, as indicated by RMSD, RMSF, Rg, SASA, hydrogen bond interactions, PCA, DCCM analyses. Binding free energy calculations MM/PBSA method showed -28.76 kcal/mol mTYR -22.23 TYRP1, outperforming standard co-crystal such tropolone (-12.47 kcal/mol) kojic acid (-12.73 kcal/mol). Critical residues, including VAL-283 HIS-263 HIS-381, GLY-389, THR-391 were identified key contributors binding, corroborating findings displaying strong correlations Collectively, these suggest highly promising candidate treatment disorders, offering significant inhibitory activity.

Язык: Английский

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Melanin deposition and key molecular features in Xenopus tropicalis oocytes

BMC Biology, Год журнала: 2025, Номер 23(1)

Опубликована: Фев. 27, 2025

Melanin pigmentation in oocytes is a critical feature for both the esthetic and developmental aspects of oocytes, influencing their polarity overall development. Despite substantial knowledge melanogenesis melanocytes retinal pigment epithelium cells, molecular mechanisms underlying oocyte remain largely unknown. Here, we compare wild-type, tyr−/− mitf−/− Xenopus tropicalis found that exhibit normal melanin deposition at animal pole, whereas show no this site. Transmission electron microscopy confirmed proceeds normally, similar to wild-type oocytes. Transcriptomic analysis revealed still express melanogenesis-related genes, enabling them complete melanogenesis. Additionally, expression MiT subfamily factor tfe3 relatively high, while tfeb, mitf, tfec levels are extremely low. The pattern tyr other genes. Thus, independent Mitf may be regulated by factors such as Tfe3, which control genes like tyr, dct, tyrp1. Furthermore, transcriptomic data changes related mitochondrial cloud formation represent most significant during Overall, these findings suggest further elucidation Tyr-dependent Mitf-independent pole will enhance our understanding Oogenesis.

Язык: Английский

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Achieving Sustained Hair Repigmentation in Poliosis Circumscripta: Retreatment Outcomes Using Exosomes and Fractional Picosecond Laser

Journal of Cosmetic Dermatology, Год журнала: 2025, Номер 24(3)

Опубликована: Март 1, 2025

Язык: Английский

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Piceatannol—Can It Be Used to Treat Hyperpigmentation of the Skin?

Pigment Cell & Melanoma Research, Год журнала: 2025, Номер 38(2)

Опубликована: Март 1, 2025

ABSTRACT Over the years, cosmetic industry has shifted its focus from synthtic to natural compounds. This change is driven not only by safety profile of ingredients but also increased consumer awareness about products they use. As a result, many skincare have been launched in recent years. Hyperpigmentation disorders, such as melasma, age spots (solar lentigines), post‐inflammatory hyperpigmentation, freckles, and acanthosis nigricans, are significant concerns. These conditions pose pathological issues affect individuals' self‐esteem. Consequently, treating hyperpigmentation reducing melanogenesis become key area interest cosmetology. Among various compounds, piceatannol (PCT) shows great potential hyperpigmentation. The primary mechanism previously explored inhibition tyrosinase enzyme, which one most researched strategies for combating melanogenesis. Additionally, PCT shown downregulate MITF expression, gene responsible transcription melanogenic proteins enzymes. However, beyond these two mechanisms, little known how may inhibit In this review, we aim bridge that gap. We will explore speculate on possible upstream signaling pathways MITF, Nrf, FOXO3a, CREB, MAPK signaling, etc., where could potentially act review pave way future research related highlight be targeted developing cosmetics treatments disorders.

Язык: Английский

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Integrated analysis of tyrosine-induced MiRNA and mRNA expression profiles in melanocytes reveals the regulatory role of miR-1560-3p in melanin deposition in Xichuan black-bone chickens

BMC Genomics, Год журнала: 2025, Номер 26(1)

Опубликована: Апрель 7, 2025

Tyrosine is a prerequisite for melanin biosynthesis and plays crucial role in the growth development of melanocytes, but underlying mechanism still unclear. In our previous research, we added 10- 9-10- 6 mol/L tyrosine to melanocytes black-bone chickens found that significantly increased tyrosinase content melanocytes. this study, from Xichuan were used as research objects, was transcriptome sequencing. By analyzing miRNA mRNA expression profiles, miRNA-mRNA network constructed, targeting relationship demonstrated by double luciferase reporting experiments, influence tyrosine-mediated on deposition verified constructing overexpression interference vectors. We promoted proliferation migration profile analysis identified 57 differentially expressed mRNAs (DEGs) 19 miRNAs (DEMs). Fifty miRNA‒mRNA target gene pairs via coexpression DEGs DEMs predicted various genes. Notably, VIP reported be involved melanoma cells. The binding miR-1560-3p further validated dual-luciferase reporter assay. addition, test confirmed inhibited reduced content. conclusion, may affects affecting miR-1560-3p-VIP axis. results study provide experimental evidence elucidating chickens, serve reference future investigations.

Язык: Английский

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Genetics of hair graying with age

Ageing Research Reviews, Год журнала: 2023, Номер 89, С. 101977 - 101977

Опубликована: Июнь 3, 2023

Hair graying is an early and obvious phenotypic physiological trait with age in humans. Several recent advances molecular biology genetics have increased our understanding of the mechanisms hair graying, which elucidate genes related to synthesis, transport, distribution melanin follicles, as well regulating these processes above. Therefore, we review examine trends genetic aspects from enrichment theory, Genome-Wide association studies, whole exome sequencing, gene expression animal models for age, aiming overview changes at level establish foundation future research. Meanwhile, by summarizing genetics, it's great value explore possible mechanism, treatment, or even prevention age.

Язык: Английский

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Exosomes Derived from Hypertrophic Scar Fibroblasts Suppress Melanogenesis in Normal Human Epidermal Melanocytes

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(13), С. 7236 - 7236

Опубликована: Июнь 30, 2024

Post-burn hypertrophic scars often exhibit abnormal pigmentation. Exosomes play important roles in maintaining normal physiological homeostasis and the pathological development of diseases. This study investigated effects exosomes derived from scar fibroblasts (HTSFs) on melanocytes, which are pigment-producing cells. Normal (NFs) HTSFs were isolated cultured skin (HTS) tissue. Both NF- HTSF-exosomes a cell culture medium purified using column-based technique. The human epidermal melanocytes treated with both at concentration 100 μg/mL different times. proliferation, melanin content medium, apoptotic factors, transcription synthesis enzymes, signaling, signal transduction pathways, activators factors (STAT) 1, 3, 5, 6 investigated. Compared Dulbecco's phosphate-buffered saline (DPBS)-treated controls NF-exosomes, decreased melanocyte proliferation secretion. molecular patterns apoptosis, synthesis, Smad non-Smad STATs altered by treatment HTSF-exosomes. No significant differences observed between DPBS-treated control NF-exosome-treated HTSF-derived may role hypopigmentation patients HTS.

Язык: Английский

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