Cell Biochemistry and Biophysics, Год журнала: 2023, Номер 81(4), С. 673 - 682
Опубликована: Сен. 26, 2023
Язык: Английский
Advances in respiratory medicine, Год журнала: 2023, Номер 91(1), С. 26 - 48
Опубликована: Янв. 31, 2023
The respiratory system is a well-organized multicellular organ, and disruption of cellular homeostasis or abnormal tissue repair caused by genetic deficiency exposure to risk factors lead life-threatening pulmonary disease including idiopathic fibrosis (IPF). Although there no clear etiology as the name reflected, its pathological progress closely related uncoordinated molecular signals. Here, we review advances in our understanding role lung cells IPF pathology epithelial cells, mesenchymal stem fibroblasts, immune endothelial cells. These summarize various cell components signaling pathways pathogenesis fibrosis, which helpful further study mechanism disease, provide new opportunities for prevention treatment, expected improve survival rate quality life patients.
Язык: Английский
Процитировано
22
Опубликована: Янв. 1, 2025
Язык: Английский
Процитировано
0
Journal of Ethnopharmacology, Год журнала: 2023, Номер 318, С. 116836 - 116836
Опубликована: Июль 4, 2023
Язык: Английский
Процитировано
10
British Journal of Pharmacology, Год журнала: 2023, Номер 180(23), С. 3008 - 3023
Опубликована: Июль 10, 2023
Abstract Background and Purpose Nitazoxanide is a therapeutic anthelmintic drug. Our previous studies found that nitazoxanide its metabolite tizoxanide activated adenosine 5′‐monophosphate‐activated protein kinase (AMPK) inhibited signal transducer activator of transcription 3 (STAT3) signals. As AMPK activation and/or STAT3 inhibition are targets for treating pulmonary fibrosis, we hypothesized would be effective in experimental fibrosis. Experimental Approach The mitochondrial oxygen consumption rate cells was measured by using the high‐resolution respirometry system Oxygraph‐2K. membrane potential evaluated tetramethyl rhodamine methyl ester (TMRM) staining. target levels were western blotting. mice fibrosis model established through intratracheal instillation bleomycin. examination lung tissues changes carried out haematoxylin eosin (H&E), Masson Key Results signalling human fibroblast (MRC‐5 cells). transforming growth factor‐β1 (TGF‐β1)‐induced proliferation migration MRC‐5 cells, collagen‐I α‐smooth muscle cell actin (α‐SMA) expression, secretion from cells. epithelial–mesenchymal transition (EMT) TGF‐β1‐induced Smad2/3 mouse epithelial (MLE‐12 Oral administration reduced bleomycin‐induced and, mice, Delayed treatment attenuated progression. Conclusions Implications improves suggesting application clinic.
Язык: Английский
Процитировано
10
Chinese Medicine, Год журнала: 2023, Номер 18(1)
Опубликована: Авг. 3, 2023
Abstract Pulmonary fibrosis is a chronic progressive interstitial lung disease caused by variety of etiologies. The can eventually lead to irreversible damage the tissue structure, severely affecting respiratory function and posing serious threat human health. Currently, glucocorticoids immunosuppressants are main drugs used in clinical treatment pulmonary fibrosis, but their efficacy limited they cause adverse effects. Traditional Chinese medicines have important research value potential for application anti-pulmonary fibrosis. In recent years, more scientific researches been conducted on use traditional medicine improve or reduce some breakthroughs made. This review paper systematically summarized progress pharmacological mechanism active compounds improving reducing We systematic search several databases, including PubMed, Web Science, Google Scholar, using keywords such as idiopathic pneumonia, natural products, herbal medicine, therapeutic methods. Ultimately, 252 articles were included evaluated this analysis. anti-fibrotic mechanisms these studies be roughly categorized into 5 aspects, inhibition epithelial-mesenchymal transition, anti-inflammatory antioxidant effects, improvement extracellular matrix deposition, mediation apoptosis autophagy, endoplasmic reticulum stress. purpose article provide pharmaceutical researchers with information reference further research.
Язык: Английский
Процитировано
8
Frontiers in Plant Science, Год журнала: 2023, Номер 14
Опубликована: Сен. 28, 2023
Atractylodes lancea is widely distributed in East Asia, ranging from Amur to south-central China. The rhizome of A. commonly used traditional Chinese medicine, however, the quality products varies across different regions with geochemical characteristics.This study aimed identify chemotypes areas and screen for chemical markers by quantifying volatile organic compounds (VOCs) using a targeted metabolomics approach based on GC-MS/MS.The Hubei, Anhui, Shaanxi, region west Henan province was classified as Hubei Chemotype (HBA). HBA characterized high content β-eudesmol hinesol lower levels atractylodin atractylon. In contrast, Maoshan (MA) Jiangsu, Shandong, Shanxi, Hebei, Inner Mongolia, other northern regions, exhibited A total 15 categories VOCs metabolites were detected identified, revealing significant differences profiles terpenoid, heterocyclic compound, ester, ketone among areas. Multivariate statistics indicated that 6 455 could serve candidate differentiating obtained southern, northern, areas.This comprehensive analysis provides fingerprint selected lancea. Our results highlight potential metabolite profiling combined chemometrics authenticating geographical origin
Язык: Английский
Процитировано
8
Naunyn-Schmiedeberg s Archives of Pharmacology, Год журнала: 2024, Номер unknown
Опубликована: Март 27, 2024
Abstract Targeting lung cancer stem cells (LC-SCs) for metastasis may be an effective strategy against cancer. This study is the first on epithelial-mesenchymal transition (EMT) properties of boric acid (BA) in LC-SCs. LC-SCs were isolated using magnetic cell sorting (MACS) method. Tumor-sphere formation and flow cytometry confirmed CSC phenotype. The cytotoxic effect BA was measured by MTT analysis, EMT examined migration analysis. expression levels ZEB1, SNAIL1, ITGA5, CDH1, ITGB1, VIM, COL1A1, LAMA5 genes analyzed RT-qPCR. E-cadherin, Collagen-1, MMP-3, Vimentin expressions immunohistochemically. Boric slightly reduced cells. Increased transcription factor SNAIL ( p < 0.001), but not observed mRNA ITGB1 0.01), ITGA5 COL1A1 0.001) increased; CDH1 VIM decreased Moreover, while E-cadherin Collagen-1 0.01) immunoreactivities significantly increased, MMP-3 BA-treated To conclude, current provided insights into efficacy effects regarding proliferation, EMT, death future studies. Graphical abstract
Язык: Английский
Процитировано
2
Molecules, Год журнала: 2022, Номер 27(9), С. 2946 - 2946
Опубликована: Май 5, 2022
Atractylodin (ATR) has anticancer effects on some tumor cells by inducing apoptosis, but its mechanism in lung cancer remains unclear. This study investigates the inhibitory effect of ATR A549 cells. Cell viability was detected Counting Kit-8 assay, and results showed that could significantly inhibit proliferation Apoptosis Annexin V-FITC/PI staining, apoptosis rate mitochondrial membrane potential were flow cytometry. Results negatively correlated with change potential. Western blot analysis regulated induced mitogen-activated protein kinase, signal transducer activator transcription 3, nuclear factor kappa B signaling pathways. Analyses reactive oxygen species (ROS), cell cycle, migration intracellular ROS accumulation as an initiation to induce cycle arrest AKT pathway inhibition Wnt pathway. can proliferation, arrest, (p < 0.05 considered statistically significant, * p 0.05, ** 0.01 *** 0.001).
Язык: Английский
Процитировано
9
European Journal of Pharmacology, Год журнала: 2023, Номер 959, С. 176088 - 176088
Опубликована: Сен. 28, 2023
Язык: Английский
Процитировано
4
Biological and Pharmaceutical Bulletin, Год журнала: 2024, Номер 47(6), С. 1179 - 1188
Опубликована: Июнь 13, 2024
Secondary lymphedema occurs after cancer surgery involving lymph node dissection owing to the lymphatic system dysfunction. However, pathophysiology of and molecular pathways involved remain unknown. This study aimed develop a rat hindlimb model investigate mechanisms that drive effects traditional Japanese medicine goreisan on lymphedema. The was induced by combination surgeries popliteal dissection, skin cautery incision, fascial ablation coagulation in right using male Wistar rats. foot volume significantly increased, recovery delayed surgeries. Dermal thickness dilated vessels were observed postoperative day 2. number infiltrating leukocytes (CD45+ cells), including CD4+ T-cells, increased group compared with sham group. relative mRNA expression protein levels interleukin-6 (IL-6), CC chemokine ligand 2 (CCL2), transforming growth factor β1 (TGF-β1), Fms-related receptor tyrosine kinase 4 (FLT4) higher than Foot decreased goreisan, furosemide, prednisolone treatments. Goreisan diminished increase same trend for CCL2 FLT4 expression. In conclusion, this exhibited volume, skin-infiltrating cells, pathological changes accompanied inflammatory fibrotic responses, suggesting presented significant clinical features may exert therapeutic effect inhibiting T-cell infiltration.
Язык: Английский
Процитировано
1