Biological impact and therapeutic perspective of targeting PI3K/Akt signaling in hepatocellular carcinoma: Promises and Challenges

Pharmacological Research, Год журнала: 2022, Номер 187, С. 106553 - 106553

Опубликована: Ноя. 16, 2022

Cancer progression results from activation of various signaling networks. Among these, PI3K/Akt contributes to proliferation, invasion, and inhibition apoptosis. Hepatocellular carcinoma (HCC) is a primary liver cancer with high incidence rate, especially in regions prevalence viral hepatitis infection. Autoimmune disorders, diabetes mellitus, obesity, alcohol consumption, inflammation can also lead initiation development HCC. The treatment HCC depends on the identification oncogenic factors that tumor cells develop resistance therapy. present review article focuses role progression. Activation promotes glucose uptake, favors glycolysis increases cell proliferation. It inhibits both apoptosis autophagy while promoting survival. stimulates epithelial-to-mesenchymal transition (EMT) matrix-metalloproteinase (MMP) expression during metastasis. In addition increasing colony formation capacity facilitating spread cells, angiogenesis. Therefore, silencing prevents aggressive behavior. confer drug resistance, particularly sorafenib, decreases radio-sensitivity cells. Anti-cancer agents, like phytochemicals small molecules suppress by limiting Being upregulated tissues clinical samples, be used as biomarker predict patients' response

Язык: Английский

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LncRNA-miRNA axis in tumor progression and therapy response: An emphasis on molecular interactions and therapeutic interventions

Biomedicine & Pharmacotherapy, Год журнала: 2022, Номер 154, С. 113609 - 113609

Опубликована: Авг. 27, 2022

Epigenetic factors are critical regulators of biological and pathological mechanisms they could interact with different molecular pathways. Targeting epigenetic has been an idea approach in disease therapy, especially cancer. Accumulating evidence highlighted function long non-coding RNAs (lncRNAs) as cancer initiation development focused on their association downstream targets. microRNAs (miRNAs) the most well-known targets lncRNAs present review focuses lncRNA-miRNA axis malignancy therapy resistance tumors. LncRNA-miRNA regulates cell death such apoptosis autophagy cancers. This affects tumor metastasis via regulating EMT MMPs. Besides, determines sensitivity cells to chemotherapy, radiotherapy immunotherapy. Based studies, can be affected by drugs genetic tools this may affect expression level miRNAs targets, leading suppression/progression. LncRNAs have both tumor-promoting tumor-suppressor functions unique complicated implication therapy. also other signaling networks PI3K/Akt, STAT3, Wnt/β-catenin EZH2 among others. Notably, lncRNA/miRNA considered a signature for diagnosis prognosis

Язык: Английский

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Extracellular Vesicles Derived from Glioma Stem Cells Affect Glycometabolic Reprogramming of Glioma Cells Through the miR-10b-5p/PTEN/PI3K/Akt Pathway

Stem Cell Reviews and Reports, Год журнала: 2024, Номер 20(3), С. 779 - 796

Опубликована: Янв. 31, 2024

Язык: Английский

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Investigation of the Impact of Magnetic Resonance Imaging-assisted Surgery on Immune Cell Cytokine Levels and Efficacy in Patients with Gliomas

Current Problems in Surgery, Год журнала: 2024, Номер 63, С. 101640 - 101640

Опубликована: Окт. 18, 2024

Язык: Английский

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The Lncrna Bbox1-As1 Regulates the Mir-382-5p/Cbx3 Signalling Pathway to Affect the Proliferation and Apoptosis of Glioblastoma Cells

Опубликована: Янв. 1, 2025

Язык: Английский

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Oncogenic roles and related mechanisms of the long non-coding RNA MINCR in human cancers

Frontiers in Cell and Developmental Biology, Год журнала: 2023, Номер 11

Опубликована: Май 5, 2023

Long non-coding RNAs (lncRNAs) play vital roles in regulating epigenetic mechanisms and gene expression levels, their dysregulation is closely associated with a variety of diseases such as cancer. Several studies have demonstrated that lncRNAs are dysregulated during tumor progression. Recently, the MYC-induced long RNA MINCR, newly identified lncRNA, has been to act an oncogene different cancers, including gallbladder cancer, hepatocellular colorectal non-small cell lung oral squamous carcinoma, nasopharyngeal glioma. Moreover, MINCR reported biomarker prognosis patients cancers. In this review, we summarize analyze oncogenic human cancers terms its clinical significance, biological functions, cellular activities, regulatory mechanism. Our analysis literature suggests potential novel therapeutic target

Язык: Английский

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lncRNA TUSC7 sponges miR-10a-5p and inhibits BDNF/ERK pathway to suppress glioma cell proliferation and migration

Aging, Год журнала: 2023, Номер unknown

Опубликована: Апрель 14, 2023

Objective: Gliomas as primary cerebral malignancies frequently occurring in adults have relatively high morbidity and mortality. The underlying role of long non-coding ribonucleic acids (lncRNAs) has attracted much attention, among which tumor suppressor candidate 7 (TUSC7) is a novel gene whose regulatory mechanism human gliomas remains inconclusive. Methods results: In this study, bioinformatics analysis indicated that TUSC7 could specifically bind to microRNA (miR)-10a-5p, according quantitative polymerase chain reaction (q-PCR), miR-10a-5p was up-regulated glioma cells negatively correlated with expression. Dual-luciferase reporter assay showed the ability miR-10a-5p, overexpression notably inhibited expression, restrained cell proliferation migration, regulated cycle cyclin expression via brain-derived neurotrophic factor/extracellular signal-regulated kinase (BDNF/ERK) pathway. inhibitory effect on also verified by designing knockdown panels for wound healing, Transwell Western blotting assays. Conclusions: suppresses migration modulating inhibiting BDNF/ERK pathway, thus acting gliomas.

Язык: Английский

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Transcriptome Analysis and Single-Cell Sequencing Analysis Constructed the Ubiquitination-Related Signature in Glioma and Identified USP4 as a Novel Biomarker

Frontiers in Immunology, Год журнала: 2022, Номер 13

Опубликована: Июнь 14, 2022

Background Glioma, the most frequent malignant tumor of neurological system, has a poor prognosis and treatment problems. Glioma’s microenvironment is also little known. Methods We downloaded glioma data from TCGA database. The patients in database were split into two groups, one for training other validation. ubiquitination genes then evaluated using COX Lasso regression to create ubiquitination-related signature. assessed signature’s predictive usefulness role immune after it was generated. Finally, vitro experiment utilized check expression function key gene, USP4. Results This signature can be used categorize patients. Glioma separated high-risk low-risk groups both validation cohorts, with group having significantly worse (P<0.05). Following further investigation microenvironment, discovered that this risk grouping could serve as guide immunotherapy. activity, invasion migration capacity, colony formation ability U87-MG LN229 cell lines drastically reduced important gene USP4 knocked down tests. Overexpression A172 line, on hand, greatly improved clonogenesis, migration. Conclusions Our research established foundation understanding gliomas identified possible biomarker.

Язык: Английский

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Polysaccharides Extracted from Angelica sinensis (Oliv.) Diels Relieve the Malignant Characteristics of Glioma Cells through Regulating the MiR-373-3p-Mediated TGF-β/Smad4 Signaling Pathway

Evidence-based Complementary and Alternative Medicine, Год журнала: 2022, Номер 2022, С. 1 - 8

Опубликована: Июль 9, 2022

Angelica sinensis polysaccharide (ASP) is a traditional herbal medicine accompanied by antitumor potential. This study aims to explore the therapeutic potential of ASP on glioma, as well underlying mechanisms involving microRNA-373-3p (miR-373-3p) and TGF-β/Smad4 signaling pathway.U251 cells (a human glioma cell line) were treated with different concentrations ASP. miR-373-3p was silenced in U251 transfection inhibitor. Cell viability apoptosis measured CCK-8 assay flow cytometry, respectively. migration invasion detected wound healing transwell assays, The expression RT-qPCR. protein expressions TGF-β Smad4 evaluated both western blotting immunofluorescence.ASP inhibited viability, migration, invasion, enhanced dose-dependent manner. increased decreased cells. Silencing weakened effects inhibiting promoting apoptosis. In addition, deleting pathway cells.ASP suppresses malignant progression via regulating miR-373-3p-mediated pathway.

Язык: Английский

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Down-regulation of CX43 expression by miR-1 inhibits the proliferation and invasion of glioma cells

Translational Cancer Research, Год журнала: 2022, Номер 11(11), С. 4126 - 4136

Опубликована: Ноя. 1, 2022

Connexin (CX) 43 makes glioblastoma resistant to temozolomide, the first-line chemotherapy drug. However, targeting CX43 is very difficult because mechanisms underlying CX43-mediated resistance remain unclear. highly expressed in glioblastoma, which closely associated with poor prognosis and resistance. The present study was analyze mechanism of microRNA (miR)-1 regulating proliferation invasion glioma cells.The effects knockdown miR-1 on growth cell lines were observed by establishing blank, inhibitor, mimic groups. Cell detected using a Counting Kit-8 (CCK-8) assay, apoptosis flow cytometry, protein expression western blot. We used Student's t-test assess continuous data between two groups Kruskal-Wallis test adopted for multiple group comparisons.Compared mimics normal control (NC) group, rate miR-1-3p decreased, while that inhibitor increased compared NC group. In addition, model U251 cells exerted an inhibitory effect ability cells, whereas showed invasion-promoting effect. dual-luciferase assay had targeted relationship gene.Down-regulation inhibited infiltration further promoted expression.

Язык: Английский

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